LOCUS       sequence-346475-        6539 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  KSHV pre-miRNA K12-2wt.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 6539)
  AUTHORS   Alzhanova D, Corcoran K, Bailey AG, Long K, Taft-Benz S, Graham RL, 
            Broussard GS, Heise M, Neumann G, Halfmann P, Kawaoka Y, Baric RS, 
            Damania B, Dittmer DP
  TITLE     Novel modulators of p53-signaling encoded by unknown genes of 
            emerging viruses.
  JOURNAL   PLoS Pathog. 2021 Jan 7;17(1):e1009033. doi: 
            10.1371/journal.ppat.1009033. eCollection 2021 Jan.
  PUBMED    33411764
REFERENCE   2  (bases 1 to 6539)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS 
            Pathog."; date: "2021-01-7"; volume: "17(1):e1009033. doi"; pages: "
            10.1371/journal.ppat.1009033. eCollection 2021 Jan"
COMMENT     Sequence Label: pOME0207
FEATURES             Location/Qualifiers
     source          1..6539
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     enhancer        66..368
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        369..572
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     regulatory      611..620
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             617..1294
                     /codon_start=1
                     /product="rapidly maturing tetrameric variant of DsRed 
                     fluorescent protein (Bevis and Glick, 2002)"
                     /label=DsRed-Express
                     /note="mammalian codon-optimized"
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGSFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLYPRDGVLKGEIHK
                     ALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDSKLDITSHNEDYTIVEQYERAEGRHH
                     LFL"
     primer_bind     complement(747..765)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     complement(797..817)
                     /label=DsRed1-N
                     /note="DsRed1, reverse primer"
     primer_bind     1015..1034
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     1209..1232
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     LTR             1414..1839
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     Moloney murine leukemia virus"
     primer_bind     complement(1942..1964)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(2100..2120)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        2105..2433
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      2285..2419
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     2455..2474
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(2555..2572)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2726..3314)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2806..2825)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3485..4276,4277..4345))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4108..4127
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4346..4450)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     4518..4536
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     enhancer        4636..5014
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        5015..5218
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             5219..5394
                     /label=5' LTR (truncated)
                     /note="truncated long terminal repeat from Moloney murine 
                     sarcoma virus"
     misc_feature    5457..5656
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             5856..6272
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     6202..6224
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     6240..6256
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     promoter        6296..6536
                     /label=U6 promoter
                     /note="Truncated RNA polymerase III promoter for human U6 
                     snRNA used in pSIREN vectors such as 
                     pSIREN-RetroQ-ZsGreen1. Expected transcription start site 
                     is 8bp downstream of this feature."
     primer_bind     6296..6316
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     6467..6486
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
ORIGIN
        1 aattctagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag
       61 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc
      121 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga
      181 cgtcaatggt ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata
      241 tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc
      301 agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta
      361 ttaccatggt gatgcggttt tggcagtaca tcaatgggcg tggatagcgg tttgactcac
      421 ggggatttcc aagtctccac cccattgacg tcaatgggag tttgttttgg caccaaaatc
      481 aacgggactt tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc
      541 gtgtacggtg ggaggtctat ataagcagag ctggtttagt gaaccgtcag atccgctagc
      601 gctaccggtc gccaccatgg cctcctccga ggacgtcatc aaggagttca tgcgcttcaa
      661 ggtgcgcatg gagggctccg tgaacggcca cgagttcgag atcgagggcg agggcgaggg
      721 ccgcccctac gagggcaccc agaccgccaa gctgaaggtg accaagggcg gccccctgcc
      781 cttcgcctgg gacatcctgt ccccccagtt ccagtacggc tccaaggtgt acgtgaagca
      841 ccccgccgac atccccgact acaagaagct gtccttcccc gagggcttca agtgggagcg
      901 cgtgatgaac ttcgaggacg gcggcgtggt gaccgtgacc caggactcct ccctgcagga
      961 cggctccttc atctacaagg tgaagttcat cggcgtgaac ttcccctccg acggccccgt
     1021 aatgcagaag aagactatgg gctgggaggc ctccaccgag cgcctgtacc cccgcgacgg
     1081 cgtgctgaag ggcgagatcc acaaggccct gaagctgaag gacggcggcc actacctggt
     1141 ggagttcaag tccatctaca tggccaagaa gcccgtgcag ctgcccggct actactacgt
     1201 ggactccaag ctggacatca cctcccacaa cgaggactac accatcgtgg agcagtacga
     1261 gcgcgccgag ggccgccacc acctgttcct gtagctcgag atatcagtgg tccaggctct
     1321 agttttgact caacaatatc accagctgaa gcctatagag tacgagccat agataaaata
     1381 aaagatttta tttagtctcc agaaaaaggg gggaatgaaa gaccccacct gtaggtttgg
     1441 caagctagct taagtaacgc cattttgcaa ggcatggaaa aatacataac tgagaataga
     1501 gaagttcaga tcaaggtcag gaacagatgg aacagggtcg accctagaga accatcagat
     1561 gtttccaggg tgccccaagg acctgaaatg accctgtgcc ttatttgaac taaccaatca
     1621 gttcgcttct cgcttctgtt cgcgcgcttc tgctccccga gctcaataaa agagcccaca
     1681 acccctcact cggggcgcca gtcctccgat tgactgagtc gcccgggtac ccgtgtatcc
     1741 aataaaccct cttgcagttg catccgactt gtggtctcgc tgttccttgg gagggtctcc
     1801 tctgagtgat tgactacccg tcagcggggg tctttcattt gggggctcgt ccgggatcgg
     1861 gagacccctg cccagggacc accgacccac caccgggagg taagctggct gcctcgcgcg
     1921 tttcggtgat gacggtgaaa acctctgaca catgcagctc ccggagacgg tcacagcttg
     1981 tctgtaagcg gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg
     2041 gtgtcggggc gcagccatga cccagtcacg tagcgatagc ggagtgtaga tccggctgtg
     2101 gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca
     2161 aagcatgcat ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg
     2221 cagaagtatg caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc
     2281 gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat
     2341 tttttttatt tatgcagagg ccgaggcgcc tcggcctctg agctattcca gaagtagtga
     2401 ggaggctttt ttggaggcct aggcttttgc aaaaagctta ctggcttaac tatgcggcat
     2461 cagagcagat tgtactgaga gtgcaccata tgcggtgtga aataccgcac agatgcgtaa
     2521 ggagaaaata ccgcatcagg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg
     2581 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag
     2641 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc
     2701 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
     2761 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
     2821 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
     2881 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
     2941 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
     3001 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
     3061 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
     3121 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta
     3181 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
     3241 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
     3301 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
     3361 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc
     3421 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg
     3481 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat
     3541 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg
     3601 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa
     3661 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca
     3721 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc
     3781 gcaacgttgt tgccattgct gcaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt
     3841 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa
     3901 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat
     3961 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct
     4021 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga
     4081 gttgctcttg cccggcgtca acacgggata ataccgcgcc acatagcaga actttaaaag
     4141 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga
     4201 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca
     4261 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg
     4321 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc
     4381 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag
     4441 gggttccgcg cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc attattatca
     4501 tgacattaac ctataaaaat aggcgtatca cgaggccctt tcgtcttcaa gaattagctt
     4561 ggccattgca tacgttgtat ccatatcata atatgtacat ttatattggc tcatgtccaa
     4621 cattaccgcc atgttgacat tgattattga ctagttatta atagtaatca attacggggt
     4681 cattagttca tagcccatat atggagttcc gcgttacata acttacggta aatggcccgc
     4741 ctggctgacc gcccaacgac ccccgcccat tgacgtcaat aatgacgtat gttcccatag
     4801 taacgccaat agggactttc cattgacgtc aatggtggag tatttacggt aaactgccca
     4861 cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg tcaatgacgg
     4921 taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc ctacttggca
     4981 gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc agtacatcaa
     5041 tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa
     5101 tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta acaactccgc
     5161 cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa gcagagctca
     5221 ataaaagagc ccacaacccc tcactcggcg cgccagtctt ccgatagact gcgtcgcccg
     5281 ggtacccgta ttcccaataa agcctcttgc tgtttgcatc cgaatcgtgg tctcgctgtt
     5341 ccttgggagg gtctcctctg agtgattgac tacccacgac gggggtcttt catttggggg
     5401 ctcgtccggg atttggagac ccctgcccag ggaccaccga cccaccaccg ggaggtaagc
     5461 tggccagcaa cttatctgtg tctgtccgat tgtctagtgt ctatgtttga tgttatgcgc
     5521 ctgcgtctgt actagttagc taactagctc tgtatctggc ggacccgtgg tggaactgac
     5581 gagttctgaa cacccggccg caaccctggg agacgtccca gggactttgg gggccgtttt
     5641 tgtggcccga cctgaggaag ggagtcgatg tggaatccga ccccgtcagg atatgtggtt
     5701 ctggtagaga cgagaaccta aaacagttcc cgcctccgtc tgaatttttg ctttcggttt
     5761 ggaaccgaag ccgcgcgtct tgtctgctgc agcgctgcag catcgttctg tgttgtctct
     5821 gtctgactgt gtttctgtat ttgtctgaaa attagggcca gactgttacc actcccttaa
     5881 gtttgacctt aggtcactgg aaagatgtcg agcggatcgc tcacaaccag tcggtagatg
     5941 tcaagaagag acgttgggtt accttctgct ctgcagaatg gccaaccttt aacgtcggat
     6001 ggccgcgaga cggcaccttt aaccgagacc tcatcaccca ggttaagatc aaggtctttt
     6061 cacctggccc gcatggacac ccagaccagg tcccctacat cgtgacctgg gaagccttgg
     6121 cttttgaccc ccctccctgg gtcaagccct ttgtacaccc taagcctccg cctcctcttc
     6181 ctccatccgc cccgtctctc ccccttgaac ctcctcgttc gaccccgcct cgatcctccc
     6241 tttatccagc cctcactcct tctctaggcg ccggaattga agatctgggc aggaagaggg
     6301 cctatttccc atgattcctt catatttgca tatacgatac aaggctgtta gagagataat
     6361 tagaattaat ttgactgtaa acacaaagat attagtacaa aatacgtgac gtagaaagta
     6421 ataatttctt gggtagtttg cagttttaaa attatgtttt aaaatggact atcatatgct
     6481 taccgtaact tgaaagtatt tcgatttctt ggctttatat atcttgtgga aaggacgag
//
