LOCUS       sequence-346499-        2366 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  yeast MoClo level-0 part plasmid type 8b containing 5' homology for 
            integration site XI-5.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2366)
  AUTHORS   Otto M, Skrekas C, Gossing M, Gustafsson J, Siewers V, David F
  TITLE     Expansion of the Yeast Modular Cloning Toolkit for CRISPR-Based 
            Applications, Genomic Integrations and Combinatorial Libraries.
  JOURNAL   ACS Synth Biol. 2021 Dec 17;10(12):3461-3474. doi: 
            10.1021/acssynbio.1c00408. Epub 2021 Dec 3.
  PUBMED    34860007
REFERENCE   2  (bases 1 to 2366)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.1c00408"; journalName: "ACS Synth Biol"; date: 
            "2021-12-17- 17"; volume: "10"; issue: "12"; pages: "3461-3474"
COMMENT     Sequence Label: pMC8b-XI5up
FEATURES             Location/Qualifiers
     source          1..2366
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     137..156
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1068..1102)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(1173..1832)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1747..1766
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      join(2014..2366,1..236)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc
       61 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca
      121 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
      181 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
      241 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct
      301 ttcctgcgtt atcccctgat tctgtggata accgtagtcg gtctcacaat gcgctgaggg
      361 tttaatgcgg agaagtcgtt gatagcattt ccgaaggctt ttccacattg actgcagtat
      421 ctaaaagagc tccagcagcg gtcttagtag ttcctgtgct tgccgctttg cagtatacag
      481 ggacattggg aactacggag gaaaaatcgg aatgctctga gctcttctca aaaccagacg
      541 aactcgtctt atttctcagt aaattaccca acatataaac tctccctctt caccttctct
      601 acaaggccac acacaagaca gttttaacag gcaaatggta ctggaggctc tcaccaatgg
      661 ggtttaagac gctccaaaca cacgctctcc tttttttctt tatatatcgg tagcaacgaa
      721 agctagtcgc agaacgttac acactaaagc agatcaatca atcaatcctg agaacctgct
      781 tcgttcaaac catccactag aagaaacacg cacgtgtacc caaaaataga gacaaacaaa
      841 cagacaaaaa taacaatgat tcctaccgac taaagtaaaa caacactcac gtaataaaaa
      901 atcctagata caactcactt gtattcctct cttctttgac catcactaaa gcttctctct
      961 tactatattc ctcaaataaa aaaaagttgc aatgattact taccaatgtg ccataaactc
     1021 cgtgcaccaa ttaagtcctc agcgagctcg cccttgagac cagaccaata aaaaacgccc
     1081 ggcggcaacc gagcgttctg aacaaatcca gatggagttc tgaggtcatt actggatcta
     1141 tcaacaggag tccaagcgag ctcgatatca aattacgccc cgccctgcca ctcatcgcag
     1201 tactgttgta attcattaag cattctgccg acatggaagc catcacaaac ggcatgatga
     1261 acctgaatcg ccagcggcat cagcaccttg tcgccttgcg tataatattt gcccatggtg
     1321 aaaacggggg cgaagaagtt gtccatattg gccacgttta aatcaaaact ggtgaaactc
     1381 acccagggat tggctgaaac gaaaaacata ttctcaataa accctttagg gaaataggcc
     1441 aggttttcac cgtaacacgc cacatcttgc gaatatatgt gtagaaactg ccggaaatcg
     1501 tcgtggtatt cactccagag cgatgaaaac gtttcagttt gctcatggaa aacggtgtaa
     1561 caagggtgaa cactatccca tatcaccagc tcaccgtctt tcattgccat acgaaattcc
     1621 ggatgagcat tcatcaggcg ggcaagaatg tgaataaagg ccggataaaa cttgtgctta
     1681 tttttcttta cggtctttaa aaaggccgta atatccagct gaacggtctg gttataggta
     1741 cattgagcaa ctgactgaaa tgcctcaaaa tgttctttac gatgccattg ggatatatca
     1801 acggtggtat atccagtgat ttttttctcc attttagctt ccttagctcc tgaaaatctc
     1861 gataactcaa aaaatacgcc cggtagtgat cttatttcat tatggtgaaa gttggaacct
     1921 cttacgtgcc cgatcaatca tgaccaaaat cccttaacgt gagttttcgt tccactgagc
     1981 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat
     2041 ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga
     2101 gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt
     2161 tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata
     2221 cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac
     2281 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg
     2341 ttcgtgcaca cagcccagct tggagc
//
