LOCUS       sequence-346501-        9787 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Muscle specific expression of the calcium reporter GcAMP5 in 
            zebrafish. Parton lab clone APT.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9787)
  AUTHORS   Lo HP, Lim YW, Xiong Z, Martel N, Ferguson C, Ariotti N, Giacomotto 
            J, Rae J, Floetenmeyer M, Moradi SV, Gao Y, Tillu VA, Xia D, Wang H,
            Rahnama S, Nixon SJ, Bastiani M, Day RD, Smith KA, Palpant NJ, 
            Johnston WA, Alexandrov K, Collins BM, Hall TE, Parton RG
  TITLE     Cavin4 interacts with Bin1 to promote T-tubule formation and 
            stability in developing skeletal muscle.
  JOURNAL   J Cell Biol. 2021 Dec 6;220(12). pii: 212693. doi: 
            10.1083/jcb.201905065. Epub 2021 Oct 11.
  PUBMED    34633413
REFERENCE   2  (bases 1 to 9787)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol."; date: "2021-12-6"; volume: "220(12). pii: 212693. doi"; 
            pages: " 10.1083/jcb.201905065. Epub 2021 Oct 11"
COMMENT     Sequence Label: actc1b-GcAMP5
FEATURES             Location/Qualifiers
     source          1..9787
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(144..165)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     misc_feature    join(514..525,526..688)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
     primer_bind     1061..1077
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    1097..1117
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     protein_bind    5074..5098
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      5102..5111
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(5108..5116,5117..5134,5135..5137,5138..5170,5171..5173,
                     5174..5197,5198..5224,5225..5281,5282..5287,5288..5557,
                     5558..5575,5576..6010,6011..6016,6017..6460)
                     /codon_start=1
                     /product="improved fluorescent protein-based calcium sensor
                     (Chen et al., 2013)"
                     /label=GCaMP6f
                     /translation="MGSHHHHHHGMASMTGGQQMGRDLYDDDDKDLATMVDSSRRKWNK
                     TGHAVRAIGRLSSLENVYIKADKQKNGIKANFKIRHNIEDGGVQLAYHYQQNTPIGDGP
                     VLLPDNHYLSVQSKLSKDPNEKRDHMVLLEFVTAAGITLGMDELYKGGTGGSMVSKGEE
                     LFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLT
                     YGVQCFSRYPDHMKQHDFFKSAMPEGYIQERTIFFKDDGNYKTRAEVKFEGDTLVNRIE
                     LKGIDFKEDGNILGHKLEYNLPDQLTEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRS
                     LGQNPTEAELQDMINEVDADGDGTIDFPEFLTMMARKMKYTDSEEEIREAFRVFDKDGN
                     GYISAAELRHVMTNLGEKLTDEEVDEMIREADIDGDGQVNYEEFVQMMTAK"
     CDS             join(5108..5116,5117..5134,5135..5137,5138..5170,5171..5173,
                     5174..5197,5198..5224,5225..5281,5282..5287,5288..5557,
                     5558..5575,5576..6010,6011..6016,6017..6460)
                     /codon_start=1
                     /product="improved fluorescent protein-based calcium sensor
                     (Chen et al., 2013)"
                     /label=GCaMP6s
                     /translation="MGSHHHHHHGMASMTGGQQMGRDLYDDDDKDLATMVDSSRRKWNK
                     TGHAVRAIGRLSSLENVYIKADKQKNGIKANFKIRHNIEDGGVQLAYHYQQNTPIGDGP
                     VLLPDNHYLSVQSKLSKDPNEKRDHMVLLEFVTAAGITLGMDELYKGGTGGSMVSKGEE
                     LFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLT
                     YGVQCFSRYPDHMKQHDFFKSAMPEGYIQERTIFFKDDGNYKTRAEVKFEGDTLVNRIE
                     LKGIDFKEDGNILGHKLEYNLPDQLTEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRS
                     LGQNPTEAELQDMINEVDADGDGTIDFPEFLTMMARKMKYTDSEEEIREAFRVFDKDGN
                     GYISAAELRHVMTNLGEKLTDEEVDEMIREADIDGDGQVNYEEFVQMMTAK"
     regulatory      5204..5213
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     5494..5515
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(5621..5642)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5882..5901)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     protein_bind    complement(6461..6485)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     polyA_signal    6487..6621
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    complement(6684..6704)
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     primer_bind     complement(6712..6728)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     polyA_signal    6744..6878
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     misc_feature    join(7317..7504,7505..7516)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
     primer_bind     complement(7550..7566)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7550..7566)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(7603..7623)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(7603..7621)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(7642..7658)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7655..7677)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7666..7682
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7690..7696,7697..7714,7715..7720))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7735..7756
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7873..7890)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8044..8632)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8124..8143)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8803..9594,9595..9663))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9426..9445
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9664..9768)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ccacctaaat tgtaagcgtt aatattttgt taaaattcgc gttaaatttt tgttaaatca
       61 gctcattttt taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaataga
      121 ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg
      181 actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacgc tggctaagaa
      241 ctcatcagcc tccccggtcc atctacccac gtaccaatgc accaattggc cacaatgacg
      301 gctactacat ggtgccattc cttcctcttt ataggaatgg agactacctc ctgtccaaca
      361 atgctcttgg atacgagtac gcctacctgt tggacccagg tcattgcaca acaccagaaa
      421 tgccctctga tctgcaaaag acgtgaatat ctgttcagac acccatatcc actctgttcc
      481 acacaggtca gaggtttgtc caggagttct tgacagaggt gtaaaaagta ctcaaaaatt
      541 ttactcaagt gaaagtacaa gtacttaggg aaaattttac tcaattaaaa gtaaaagtat
      601 ctggctagaa tcttacttga gtaaaagtaa aaaagtactc cattaaaatt gtacttgagt
      661 attaaggaag taaaagtaaa agcaagaaag aaaactagag attcttgttt aagcttttaa
      721 tctcaaaaaa cattaaatga aatgcataca aggttttatc ctgctttaga actgtttgta
      781 tttaattatc aaactataag acagacaatc taatgccagt acacgctact caaagttgta
      841 aaacctcaga tttaacttca gtagaagctg attctcaaaa ttgttagtgt caagcctagc
      901 tcttttgggg ctgaaaagca atcctgcagt gctgaaaagc ctctcacagg cagccgatgc
      961 gggaagaggt gtattagtct tgatagagag gctgcaaata gcaggaaacg tgagcagaga
     1021 ctccctggtg tctgaaacac aggccagatg ggccctcgag caggaaacag ctatgaccat
     1081 gattacgcca agctatcaac tttgtataga aaagttggca cgcgcagatt ccgtgtgggc
     1141 ctggtaatat cttaccattt taagctattt taagattagg gaaaaaaatt aaataaaact
     1201 actttaaaat tactttcctt aaaaagcaac atatttattt agtttttgtt taactggctc
     1261 aagattgtaa tattttactt ttacatgttt ttttaagtac ctaaatgtgt tcaaaagtaa
     1321 agtatgttat tgtttttttt ttttaatgtt agctaagctt taaattgtaa tatattactt
     1381 ttaaaagtga ctttttaaaa atgtaaataa aaaagtaact ataaatgtgt aatgcatgtt
     1441 atttctgtgg gtttttaatt ttcatctcaa ctgggcttac aattttaaca taataaaaat
     1501 caaatcaaat caaatcaggc ccttatttgt cacatacact cttgtatact gaaataaact
     1561 aaatgttgag aaaaatttaa atacaccaaa ataacgcata ccttttttgt aattacctaa
     1621 gtaaaacatt tagttccttt tttaagtgac tcaagattgt aatctattaa ttgtaaaaga
     1681 taaaactagt gatcaatctg acgtgcttct atgcagcctg tgagattaat tgtgatgttt
     1741 aattattaaa caatttaaat ggatcaaaca tttcacatgt gcttattgtg tccaacaaaa
     1801 ccagcagaag cacttaaaaa tattgcatga caacatgagt ttttaatgta aattatatat
     1861 acttcctaaa cgaagttaat catatttcta aaataataat gtcattgaaa tatttaaata
     1921 aatgataagc tttagttgat tcaacataaa attgaaagca ataaggactt ttttttttta
     1981 cagcggtgaa gcaaaagcat gtaataatca tataatataa taaataaata aataaataat
     2041 ggctcttggt gcaaatatga acactttaaa taaatataga ataaatatga tcaataagtc
     2101 ataacgatgt gttttatgtt gattataatt taaacatgtt tctaacgcaa gttttaaaaa
     2161 ctgttttaaa gtattatttt aagttataag atcaatttga ctcaacattt tgtagtgaaa
     2221 atatgttgtg taaaattaat ataatataca ctataaaaat gcagggttcc acacaataca
     2281 ttaatgttgt cccaacacaa atcgattaag ttaacaaata tatgtggatt gaacataaaa
     2341 aattaagttg tcccaatgaa atctcaagaa ttgtgttgtt tcagctcatt ttaaataagt
     2401 agttttaaca agtaaaaaat aacatatttt tgagtgtgta tatatatata ttacaaaata
     2461 taataatata aaatgtaaaa gatataaacc atgctacata tatttaagag ctattttgca
     2521 cattactttt ttgcaagcca ttttagtgtt ttttttacaa atatatatta gctaaatata
     2581 caatatctta aatatacaat taatcagaca tgcaattaat gcatttaacc tacataaaaa
     2641 tacatgatca acatatgttt tatgctgatt acaactaaat caaacgtaat gaaattaatt
     2701 tataaaagcg atttaaatat gtcaatattt taagttaaaa tcacttgaaa cctactataa
     2761 atgaacttaa attaaaaaaa tctcctttaa ttccaatgca acagtgttgt tttttcagct
     2821 gaggaccccc ggtgcagatg gttggccccc ggcccgctat tctaaggaag cctttcttaa
     2881 ttttagggcg gcgctgcatg cgctcagatc agattcacgg gtggagaggg ttattttctc
     2941 ttttttattt tttcttgcct gctcatattt gggcctgtgt gaaccccagg ctccgcctcg
     3001 gcccccagga gccgctctta tttaaagggg atcctcgcct tcagtcttcg tcccagtgga
     3061 agcatcagtc ttgtgataac aacttggtga gtcctacaga ccagcgctcg actctaggct
     3121 ttaaatgcat tgatttctct ggattagcta ccgggagagt tttaactttg cttattagtc
     3181 ttgtgctcga actgctaggt ttaaagagtg ttttctctaa tgcatgcatg aagcgacagc
     3241 tgaaaaccag aaatagagcg tttgtcctcc tgttaaactc tagcctttct ttggcatttg
     3301 tcagtccgag tcttcagctg ccggcaggag attgggtttc agtaacactt gctgagcaca
     3361 aactacacgt gctttcttca gatttaattc attattaatc attctagatt taaatgatgt
     3421 tcttactcat tgctgattat tttagccttg cggagcagat taaatgagca cgtgtttcat
     3481 taagattgat ttaaatataa ttaatatttc accactgtag ttttaaaatt gccttctaag
     3541 tcatggttga tttttaatag gtatcggctc taattagatt actattttgg tttttaaatg
     3601 ttatttctat ttcatttgta ggctcgtttt taatgtgatt ttattagatt ctagttttaa
     3661 aatcatctct tagccttaat gagcagactg aatgaacatg tttcattaag attaacttca
     3721 ttataatgaa ttatttaatc attctagtgg gttataaatc agctctattt aaattaattg
     3781 tcgtgtttat ttctatttaa tttttgatta ttttttcagg gtcgttttta tgtgattttt
     3841 tcccgtttct aattttaaac taattgtttg atttttatat gattcgactc tttttaaatg
     3901 aattgttttg ctttttttat tgagcatata tatattatac aatttcaatc aacaattgtt
     3961 gtgcttttta aatgttgttt ctatttcatt tctagggtcg attttattcg attctagttt
     4021 taaaatcgtg atttttaact tctaacctta ctgagcagac tgaatgaaca tgtttcatca
     4081 agattaactt cattatagtt aattattgaa tcgtgctagt ggtttataaa tcagctctat
     4141 ttaaattaat catcatcatt atttctattt aatttttatt atttctttag gattattttt
     4201 atgtgatttt tttcacgttc tagttttaaa ctcaatgctc attactgctc acccttgctg
     4261 agcagtcttt cgttaagatt aaattaatta ataaccattc tagttttaaa acagtgtctt
     4321 aaggcatagc tgatatttta caagtttact atttaatata tctcctttct ttttttaggg
     4381 tcgatttcat ctgatttgat taatttttat tatcttcact aagctttttg ttctaggttt
     4441 tattctcagt attattactg ttatatttga actgaatttc tcctcatagt ttattcattt
     4501 tatttattta ttttttgttt attcgctatt ttgtatttta gtttttaatt agaaatttta
     4561 ttagaatcgt tttgaactag tttttatgca ctctaacatt aatattaaat cataactatt
     4621 atttttatta taatttattt gtgtttatct ttttgtgtag ctttcatgtg tttttatttt
     4681 agttttatta tgtttgcatt gaattttttg gactagttta tataacgata tacagaaaag
     4741 ccatgacaac aaatgttttt ataataccta ttaagtataa gctaatcaga tttccactat
     4801 tctgttcaaa gttaacatta ttagccattc tgatggatca tttgacattt gtcatatgtc
     4861 tttatgaaca ctgctctttc agatgccact tttaaagctg ttttttacag taaatgaacg
     4921 tttattatta tgacattatt tcctaaggcc tctagagtac agcaggatgt ggcgtgtgca
     4981 ttaccgtgta attatgctga ctgtaaatgt gcatttatag actgtcagca tgtctgactg
     5041 tgcgctgttg tgattggtcc tgcacagacc aaggcaagtt tgtacaaaaa agcaggctgc
     5101 cgccaccatg ggttctcatc atcatcatca tcatggtatg gctagcatga ctggtggaca
     5161 gcaaatgggt cgggatctgt acgacgatga cgataaggat ctcgccacca tggtcgactc
     5221 atcacgtcgt aagtggaata agacaggtca cgcagtcaga gctataggtc ggctgagctc
     5281 actcgagaac gtctatatca aggccgacaa gcagaagaac ggcatcaagg cgaacttcaa
     5341 gatccgccac aacatcgagg acggcggcgt gcagctcgcc taccactacc agcagaacac
     5401 ccccatcggc gacggccccg tgctgctgcc cgacaaccac tacctgagcg tgcagtccaa
     5461 actttcgaaa gaccccaacg agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc
     5521 cgccgggatc actctcggca tggacgagct gtacaagggc ggtaccggag ggagcatggt
     5581 gagcaagggc gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc tggacggcga
     5641 cgtaaacggc cacaagttca gcgtgtccgg cgagggtgag ggcgatgcca cctacggcaa
     5701 gctgaccctg aagttcatct gcaccaccgg caagctgccc gtgccctggc ccaccctcgt
     5761 gaccaccctg acctacggcg tgcagtgctt cagccgctac cccgaccaca tgaagcagca
     5821 cgacttcttc aagtccgcca tgcccgaagg ctacatccag gagcgcacca tcttcttcaa
     5881 ggacgacggc aactacaaga cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa
     5941 ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc aacatcctgg ggcacaagct
     6001 ggagtacaac ctgccggacc aactgactga agagcagatc gcagaattta aagaggcttt
     6061 ctccctattt gacaaggacg gggatgggac aataacaacc aaggagctgg ggacggtgat
     6121 gcggtctctg gggcagaacc ccacagaagc agagctgcag gacatgatca atgaagtaga
     6181 tgccgacggt gacggcacaa tcgacttccc tgagttcctg acaatgatgg caagaaaaat
     6241 gaaatacaca gacagtgaag aagaaattag agaagcgttc cgtgtgtttg ataaggatgg
     6301 caatggctac atcagtgcag cagagcttcg ccacgtgatg acaaaccttg gagagaagtt
     6361 aacagatgaa gaggttgatg aaatgatcag ggaagcagac atcgatgggg atggtcaggt
     6421 aaactacgaa gagtttgtac aaatgatgac agcgaagtaa acccagcttt cttgtacaaa
     6481 gtgggggatc cagacatgat aagatacatt gatgagtttg gacaaaccac aactagaatg
     6541 cagtgaaaaa aatgctttat ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatt
     6601 ataagctgca ataaacaagt taacaacaac aattgcattc attttatgtt tcaggttcag
     6661 ggggaggtgt gggaggtttt ttccaacttt attatacata gttgataatt cactggccgt
     6721 cgttttacgg taccatcgat gatgatccag acatgataag atacattgat gagtttggac
     6781 aaaccacaac tagaatgcag tgaaaaaaat gctttatttg tgaaatttgt gatgctattg
     6841 ctttatttgt aaccattata agctgcaata aacaagttaa caacaacaat tgcattcatt
     6901 ttatgtttca ggttcagggg gaggtgtggg aggtttttta aagcaagtaa aacctctaca
     6961 aatgtggtat ggctgattat gatcctctag atcagatctg cgaagatacg gccacgggtg
     7021 ctcttgatcc tgtggctgat tttggactgt gctgctcgca gctgctgatg aatcacatac
     7081 ttcctccatt ttcttccact gattgactgt tataatttcc ctaatttcca ggtcaaggtg
     7141 ctgtgcattg tggtaataga tgtgacatga cgtcacttcc aaaggaccaa tgaacatgtc
     7201 tgaccaattt catataatgt gaaaacgatt ttcataggca gaataaataa catttaaatt
     7261 aaactgggca tcagcgcaat tcaattggtt tggtaatagc aagggaaaat agaatgaagt
     7321 gatctccaaa aaataagtac tttttgactg taaataaaat tgtaaggagt aaaaagtact
     7381 tttttttcta aaaaaatgta attaagtaaa agtaaaagta ttgattttta attgtactca
     7441 agtaaagtaa aaatccccaa aaataatact taagtacagt aatcaagtaa aattactcaa
     7501 gtactttaca cctctggttc ttgaccccct accttcagca agcccagcag atccactagt
     7561 tctagagcgg ccgccaccgc ggtggagctc cagcttttgt tccctttagt gagggttaat
     7621 tgcgcgcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac
     7681 aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt
     7741 gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc
     7801 gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg
     7861 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt
     7921 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
     7981 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
     8041 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
     8101 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
     8161 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
     8221 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
     8281 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
     8341 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
     8401 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
     8461 gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt
     8521 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
     8581 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
     8641 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aaggcatttt
     8701 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt
     8761 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag
     8821 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt
     8881 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc
     8941 gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc
     9001 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg
     9061 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac
     9121 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg
     9181 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc
     9241 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact
     9301 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc
     9361 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat
     9421 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc
     9481 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac
     9541 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa
     9601 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact
     9661 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg
     9721 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg
     9781 aaaagtg
//
