LOCUS       sequence-346513-        2245 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  yeast MoClo level-0 part plasmid type 7 containing 3' homology for 
            integration site XI-3.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2245)
  AUTHORS   Otto M, Skrekas C, Gossing M, Gustafsson J, Siewers V, David F
  TITLE     Expansion of the Yeast Modular Cloning Toolkit for CRISPR-Based 
            Applications, Genomic Integrations and Combinatorial Libraries.
  JOURNAL   ACS Synth Biol. 2021 Dec 17;10(12):3461-3474. doi: 
            10.1021/acssynbio.1c00408. Epub 2021 Dec 3.
  PUBMED    34860007
REFERENCE   2  (bases 1 to 2245)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.1c00408"; journalName: "ACS Synth Biol"; date: 
            "2021-12-17- 17"; volume: "10"; issue: "12"; pages: "3461-3474"
COMMENT     Sequence Label: pMC7-XI3dw
FEATURES             Location/Qualifiers
     source          1..2245
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     197..216
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      464..1052
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     953..972
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1763..1797)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(join(1868..2245,1..282))
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
ORIGIN
        1 aacggtgtaa caagggtgaa cactatccca tatcaccagc tcaccgtctt tcattgccat
       61 acgaaattcc ggatgagcat tcatcaggcg ggcaagaatg tgaataaagg ccggataaaa
      121 cttgtgctta tttttcttta cggtctttaa aaaggccgta atatccagct gaacggtctg
      181 gttataggta cattgagcaa ctgactgaaa tgcctcaaaa tgttctttac gatgccattg
      241 ggatatatca acggtggtat atccagtgat ttttttctcc attttagctt ccttagctcc
      301 tgaaaatctc gataactcaa aaaatacgcc cggtagtgat cttatttcat tatggtgaaa
      361 gttggaacct cttacgtgcc cgatcaatca tgaccaaaat cccttaacgt gagttttcgt
      421 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc
      481 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc
      541 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac
      601 caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac
      661 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt
      721 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct
      781 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat
      841 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt
      901 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg
      961 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt
     1021 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt
     1081 tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg
     1141 tggataaccg tagtcggtct cagagtcctg caggactagt gctgaggcat taatttacgt
     1201 ggattgagcc agcaatacag atcattatta aattgttttg tacatgatgt tagtatataa
     1261 tcgtaaagct tttctaatat gtatacctta tacatggaac tccacagaac ttgcaaacat
     1321 accaaaaatc ccttattctt gttcactcat tttacatcaa aaaataatat ttcagttatt
     1381 aaggaaaata aaaaaataga ttagagaagc attttgaaga aatagtatat tcttttattg
     1441 aacctaagag cgtgatattt ttactcgaaa taaaatacga aaaatctatg cactcatctt
     1501 tccgactact attggctcct gctcaaaaaa agagggaaaa aaagctccaa aattctatct
     1561 tttcctatcg ctcctgtcct atccttatta cgttcattac tattttaata ctatccattc
     1621 ttttattttc agtctaaaaa aaacatttct cataacggga aaagcaaaaa aatgtcaagc
     1681 ttatacatca aaacaccact gcatgcatta tctgctggtc cggattctca attaagacct
     1741 cagcgccgat gagaccagac caataaaaaa cgcccggcgg caaccgagcg ttctgaacaa
     1801 atccagatgg agttctgagg tcattactgg atctatcaac aggagtccaa gcgagctcga
     1861 tatcaaatta cgccccgccc tgccactcat cgcagtactg ttgtaattca ttaagcattc
     1921 tgccgacatg gaagccatca caaacggcat gatgaacctg aatcgccagc ggcatcagca
     1981 ccttgtcgcc ttgcgtataa tatttgccca tggtgaaaac gggggcgaag aagttgtcca
     2041 tattggccac gtttaaatca aaactggtga aactcaccca gggattggct gaaacgaaaa
     2101 acatattctc aataaaccct ttagggaaat aggccaggtt ttcaccgtaa cacgccacat
     2161 cttgcgaata tatgtgtaga aactgccgga aatcgtcgtg gtattcactc cagagcgatg
     2221 aaaacgtttc agtttgctca tggaa
//
