LOCUS       sequence-346524-        4348 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  For generating RNA probes for in situ hybridisation. Parton lab 
            clone IRZ.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4348)
  AUTHORS   Lo HP, Lim YW, Xiong Z, Martel N, Ferguson C, Ariotti N, Giacomotto 
            J, Rae J, Floetenmeyer M, Moradi SV, Gao Y, Tillu VA, Xia D, Wang H,
            Rahnama S, Nixon SJ, Bastiani M, Day RD, Smith KA, Palpant NJ, 
            Johnston WA, Alexandrov K, Collins BM, Hall TE, Parton RG
  TITLE     Cavin4 interacts with Bin1 to promote T-tubule formation and 
            stability in developing skeletal muscle.
  JOURNAL   J Cell Biol. 2021 Dec 6;220(12). pii: 212693. doi: 
            10.1083/jcb.201905065. Epub 2021 Oct 11.
  PUBMED    34633413
REFERENCE   2  (bases 1 to 4348)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol."; date: "2021-12-6"; volume: "220(12). pii: 212693. doi"; 
            pages: " 10.1083/jcb.201905065. Epub 2021 Oct 11"
COMMENT     Sequence Label: Cavin4b in pBluescript(SK+)
FEATURES             Location/Qualifiers
     source          1..4348
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    105..126
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(141..146,147..164,165..171)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    179..195
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     184..206
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     203..219
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     203..219
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     238..258
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        240..258
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(1732..1748)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1733..1749)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     complement(1773..1792)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1774..1792)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1802..1819)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(1802..1818)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1811..1833)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      1960..2415
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2047..2066)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2257..2278
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        2441..2545
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2546..2614,2615..3406)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2764..2783)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3577..4165
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4066..4085
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4319..4336
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac
       61 gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt gagttagctc
      121 actcattagg caccccaggc tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt
      181 gtgagcggat aacaatttca cacaggaaac agctatgacc atgattacgc caagcgcgca
      241 attaaccctc actaaaggga acaaaagctg gagctccacc gcggtggcgg ccgccctttt
      301 tttttttttt tttttttttt tttttttttt tttgagttca tgtgtttatt taaaaataat
      361 gatacaactg aatagtgatg gttaatcacc aaggttatac tgcgaaagct gatctttttt
      421 agtttacaag aaaatcacag aaaaggcaca gaaaaatgtt gttagaaatg actcgcctgc
      481 acaagtaatc aactacaaca tctaaactga aaaatgtcat tctaaactca tccatcgctg
      541 gacatgctgg atgtcagctg aagtccaaaa aaaaaaaaag aaatctgaat tgtgtaattc
      601 atctgtattt gtgtaaatca ttttgactgt ttggtgaaat ttcttttaaa taatattgca
      661 cacgcctaat aaataatagg ttcactcagc tctttgtctt aaaagcattt tactactaaa
      721 gaatgaataa agggatcggt gacttacaaa agaaaattgt tctcttataa tgggggactt
      781 cttgtgctgc gttcactcca gatgcagatg aagcgtcaag caagagcgat ttacatgtta
      841 ggtcatgcaa agacgagaat agacatcctg cggcgcgatt cacgcaaatg aagcggcgcg
      901 tgttgatcaa cgttgttgga caatctgaaa ttcagcagac attcgcgctg cattgaccag
      961 tcagaaattt tctcttgttg gggcgtgatt ttgatgtagc tcctgttgtt ggtgtaccga
     1021 gggaaaatcc tattaccaac accggacaac agttcatcaa actgggcttg gctttgtcgg
     1081 gagcaccgct gaaagcttcc atcatccagg tgcagtttct ggaggagttg ataaactcac
     1141 agaactgggt gcacctctga aaggatctgg tggactcaga cacagctcct aatgaatcga
     1201 cgctgcttat caaccttcct aaagcacata atcacagctg ggctctcaat aaaattcatg
     1261 ttagccattt agcaacgaag ctagagtcac cgggcagaca gaaaccctgc acatgatgca
     1321 aatccaaaag tgcgaatgaa gcgagtaagc ttacaatgtt cgtgcgtgta tttacgtatg
     1381 aatagtgtga tttatcctgg tttgatgtga acgcagcaaa ataaggatca caagaccagc
     1441 cgaacgctat tcaacatatc tcagtaatac ctctattata atacatatct gtgaaagaag
     1501 tatcttacca aacaccttta ttttatacaa cacttcatcc acacccatag gtgtcagtgt
     1561 gaccaaaaaa actaactaaa agttaaatta aaaatcatta aactagtcat ctcattatgg
     1621 tggtttgaca ttcatcaccc ttcatagcac ataacagatg taaacaaaaa tatcccccac
     1681 acctcccccc aaaagacaca tattgtttgt agaattcgat atcaagctta tcgataccgt
     1741 cgacctcgag ggggggcccg gtacccaatt cgccctatag tgagtcgtat tacgcgcgct
     1801 cactggccgt cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc caacttaatc
     1861 gccttgcagc acatccccct ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc
     1921 gcccttccca acagttgcgc agcctgaatg gcgaatggga cgcgccctgt agcggcgcat
     1981 taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag
     2041 cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc
     2101 aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc
     2161 ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt
     2221 ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa
     2281 caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg ccgatttcgg
     2341 cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat
     2401 taacgcttac aatttaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt
     2461 atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct
     2521 tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc
     2581 cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa
     2641 agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg
     2701 taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt
     2761 tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg
     2821 catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac
     2881 ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc
     2941 ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa
     3001 catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc
     3061 aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt
     3121 aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga
     3181 taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa
     3241 atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa
     3301 gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa
     3361 tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt
     3421 ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt
     3481 gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg
     3541 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt
     3601 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca
     3661 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac
     3721 tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac
     3781 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct
     3841 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg
     3901 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca
     3961 gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt
     4021 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta
     4081 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc
     4141 gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc
     4201 cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa
     4261 ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag
     4321 cgagtcagtg agcgaggaag cggaagag
//
