LOCUS       sequence-346526-        9141 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector expressing N-terminally tagged mKeima-LGALS3 
            (Lyso-Keima), for quantitative assessment of lysophagic flux.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9141)
  AUTHORS   Eapen VV, Swarup S, Hoyer MJ, Paulo JA, Harper JW
  TITLE     Quantitative proteomics reveals the selectivity of ubiquitin-binding
            autophagy receptors in the turnover of damaged lysosomes by 
            lysophagy.
  JOURNAL   eLife. 2021 Sep 29;10. pii: 72328. doi: 10.7554/eLife.72328.
  PUBMED    34585663
REFERENCE   2  (bases 1 to 9141)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "eLife."; 
            date: "2021-09-29"; volume: "10. pii: 72328. doi"; pages: " 
            10.7554/eLife.72328"
COMMENT     Sequence Label: pHAGE-mKeima-LGALS3
FEATURES             Location/Qualifiers
     source          1..9141
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     76..93
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     protein_bind    267..291
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1042..1066)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(join(1082..1741,1742..1744,1745..1747))
                     /codon_start=1
                     /product="humanized monomeric Keima-Red fluorescent 
                     protein"
                     /label=hmKeima-Red
                     /note="mammalian codon-optimized"
                     /translation="MVSVIAKQMTYKVYMSGTVNGHYFEVEGDGKGKPYEGEQTVKLTV
                     TKGGPLPFAWDILSPQLQYGSIPFTKYPEDIPDYFKQSFPEGYTWERSMNFEDGAVCTV
                     SNDSSIQGNCFIYNVKISGENFPPNGPVMQKKTQGWEPSTERLFARDGMLIGNDYMALK
                     LEGGGHYLCEFKSTYKAKKPVRMPGRHEIDRKLDVTSHNRDYTSVEQCEIAIARHSLLG
                     "
     regulatory      1744..1753
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(1829..1853)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        complement(1850..2053)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(1879..1899)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        2054..2357
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     misc_feature    2409..2526
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(2823..2867)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    3052..3285
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    3782..3907
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             3954..4587
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(4779..4798)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(4848..4864)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4848..4864)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4861..4883)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4872..4888
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4896..4902,4903..4920,4921..4926))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4941..4962
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5202..5219)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5373..5961)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5453..5472)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6132..6923,6924..6992))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6755..6774
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6993..7097)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     7165..7183
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     LTR             7302..7535
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     misc_feature    7610..8198
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             7716..7727
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     8125..8145
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8215..8814)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(8298..8318)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     8795..8814
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     misc_feature    join(8826..9141,1..237)
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    join(8828..9141,1..254)
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(8988..9007)
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
ORIGIN
        1 gggtcgctac agacgttgtt tgtcttcaag aagcttccag aggaactgct tccttcacga
       61 cattcaacag accttgcatt cctttggcga gaggggaaag acccctagga atgctcgtca
      121 agaagacagg gccaggtttc cgggccctca cattgccaaa agacggcaat atggtggaaa
      181 ataacatata gacaaacgca caccggcctt attccaagcg gcttcggcca gtaacgttag
      241 gggggggggg cggaattcgt tatcgaacca ctttgtacaa gaaagttggg tatatcatgg
      301 tatatgaagc actggtgagg tctatgtcac cagaaattcc cagtttgctg atttcattga
      361 gttttttaac ccgatgattg tactgcaaca agtgagcatc attcactgca accttgaagt
      421 ggtcaggttc aaccagtact tgtattttga atggtttccc actttcaaat gggaaaaccg
      481 actgtctttc ttcccttccc cagttattat ccagctttgt attgcaaaca atgactctcc
      541 tgttgttctc attgaagcgt gggttaaagt ggaaggcaac atcattccct ctttggaaat
      601 ctaaagcaat tctgtttgca ttgggcttca ccgtgcccag aattgttatc agcatgcgag
      661 gcaccactcc cccaggcaaa ggcaggttat aaggcacaat cagtggccca gcaggggcgc
      721 cataggggcc agtggcaggg taggctccgg gggcacttgg ctgtccagaa gatgggtagg
      781 ccccagggcc gctgggtggc cctgggtaga ctccaggtgc aggtgctccg ggataagctc
      841 caggtgctcc atggtaggcg cctggaggtg cctgtccagg ataagcccct gggggtgcct
      901 gcccggggta ggccccagga taggaagccc ctgggtagcc ccctgcccca gcaggctggt
      961 tcccccatgc gccaggccat ccttgagggt ttgggtttcc agacccagat aacgcatcat
     1021 ggagcgaaaa attgtctgcc atgccaactt ttttgtacaa acttgtttga taacctcgag
     1081 cgcccagcag ggagtggcgg gcgatggcga tctcgcactg ctccacgctg gtgtagtcgc
     1141 ggttgtggct ggtcacgtcc agcttgcggt cgatctcgtg gcggccgggc atcctcacgg
     1201 gcttcttggc cttgtaggtg ctcttgaact cgcacaggta gtggccgccg ccctccagct
     1261 tcagggccat gtagtcgttg ccgatcagca ttccgtcgcg ggcgaacagg cgctcggtgc
     1321 tgggctccca gccctgggtc ttcttctgca tcacggggcc gttggggggg aagttctcgc
     1381 cgctgatctt cacgttgtag atgaagcagt tgccctggat gctgctgtcg ttgctcacgg
     1441 tgcacacggc gccgtcctcg aagttcatgc tgcgctccca ggtgtagccc tcggggaagc
     1501 tctgcttgaa gtagtcgggg atgtcctcgg ggtacttggt gaaggggatg ctgccgtact
     1561 ggagctgggg gctcaggatg tcccaggcga agggcagggg gccacccttg gtcacggtca
     1621 gcttcacggt ctgctcgccc tcgtagggct tgcccttgcc gtcgccctcc acctcgaagt
     1681 agtggccgtt cacggtgccg ctcatgtaca ccttgtaggt catctgcttg gcgatcacgc
     1741 tcaccatggt ggcagatcaa cctcgagaga tctgcggccg ccggtgtctt ctatggaggt
     1801 caaaacagcg tggatggcgt ctccaggcga tctgacggtt cactaaacga gctctgctta
     1861 tatagacctc ccaccgtaca cgcctaccgc ccatttgcgt caatggggcg gagttgttac
     1921 gacattttgg aaagtcccgt tgattttggt gccaaaacaa actcccattg acgtcaatgg
     1981 ggtggagact tggaaatccc cgtgagtcaa accgctatcc acgcccattg atgtactgcc
     2041 aaaaccgcat caccatggta atagcgatga ctaatacgta gatgtactgc caagtaggaa
     2101 agtcccataa ggtcatgtac tgggcataat gccaggcggg ccatttaccg tcattgacgt
     2161 caataggggg cgtacttggc atatgataca cttgatgtac tgccaagtgg gcagtttacc
     2221 gtaaatactc cacccattga cgtcaatgga aagtccctat tggcgttact atgggaacat
     2281 acgtcattat tgacgtcaat gggcgggggt cgttgggcgg tcagccaggc gggccattta
     2341 ccgtaagtta tgtaacgcgg aactccacta gtccaaactg gatctctgct gtccctgtaa
     2401 taaacccgaa aattttgaat ttttgtaatt tgtttttgta attctttagt ttgtatgtct
     2461 gttgctatta tgtctactat tctttcccct gcactgtacc ccccaatccc cccttttctt
     2521 ttaaaattgt ggatgaatac tgccatttgt gaattaattc ggcgataccg tcgagatccg
     2581 ttcactaatc gaatggatct gtctctgtct ctctctccac cttcttcttc tattccttcg
     2641 ggcctgtcgg gtcccctcgg ggttgggagg tgggtctgaa acgataatgg tgaatatccc
     2701 tgcctaactc tattcactat agaaagtaca gcaaaaacta ttcttaaacc taccaagcct
     2761 cctactatca ttatgaataa ttttatatac cacagccaat ttgttatgtt aaaccaattc
     2821 cacaaacttg cccatttatc taattccaat aattcttgtt cattcttttc ttgctggttt
     2881 tgcgattctt caattaagga gtgtattaag cttgtgtaat tgttaatttc tctgtcccac
     2941 tccatccagg tcgtgtgatt ccaaatctgt tccagagatt tattactcca actagcattc
     3001 caaggcacag cagtggtgca aatgagtttt ccagagcaac cccaaatccc caggagctgt
     3061 tgatccttta ggtatctttc cacagccagg attcttgcct ggagctgctt gatgccccag
     3121 actgtgagtt gcaacagatg ctgttgcgcc tcaatagccc tcagcaaatt gttctgctgc
     3181 tgcactatac cagacaataa ttgtctggcc tgtaccgtca gcgtcattga cgctgcgccc
     3241 atagtgcttc ctgctgctcc caagaaccca aggaacaaag ctcctattcc cactgctctt
     3301 ttttctctct gcaccactct tctctttgcc ttggtgggtg ctactcctaa tggttcaatt
     3361 tttactactt tatatttata taattcactt ctccaattgt ccctcatatc tcctcctcca
     3421 ggtctgaaga tcagcggccg gccgcttgct gtgcggtggt cttacttttg ttttgctctt
     3481 cctctatctt gtctaaagct tccttggtgt cttttatctc tatcctttga tgcacacaat
     3541 agagggttgc tactgtatta tataatgatc taagttcttc tgatcctgtc tgaagggatg
     3601 gttgtagctg tcccagtatt tgtctacagc cttctgatgt ttctaacagg ccaggattaa
     3661 ctgcgaatcg ttctagctcc ctgcttgccc atactatatg ttttaattta tattttttct
     3721 ttccccctgg ccttaaccga attttttccc atcgcgatct aattctcccc cgcttaatac
     3781 tgacgctctc gcacccatct ctctccttct agcctccgct agtcaaaatt tttggcgtac
     3841 tcaccagtcg ccgcccctcg cctcttgccg tgcgcgcttc agcaagccga gtcctgcgtc
     3901 gagagagctc ctctggtttc cctttcgctt tcaagtccct gttcgggcgc cactgctaga
     3961 gattttccac actgactaaa agggtctgag ggatctctag ttaccagagt cacacaacag
     4021 acgggcacac actacttgaa gcactcaagg caagctttat tgaggcttaa gcagtgggtt
     4081 ccctagttag ccagagagct cccaggctca gatctggtct aaccagagag acccagtaca
     4141 ggcaaaaagc agctgcttat atgcaggatc tgagggctcg ccactcccca gtcccgccca
     4201 ggccacgcct ccctggaaag tccccagcgg aaagtccctt gtagcaagct cgatatcagc
     4261 agttcttgaa gtactccgga tgcagctctc gggccacgtg atgaaatgct aggcggctgt
     4321 caaacctcca ctctaacact tctctctccg ggtcatccat cccatgcagg ctcacagggt
     4381 gtaacaagct ggtgttctct cctttattgg cctcttctac cttatctggc tcaactggta
     4441 ctagcttgta gcaccatcca aaggtcagtg gatatctgac ccctggccct ggtgtgtagt
     4501 tctgctaatc agggaagtag ccttgtgtgt ggtagatcca cagatcaagg atatcttgtc
     4561 ttctttggga gtgaattagc ccttccaact actaagtttg tagtacatat ttaacaaata
     4621 caatttcttt aaaatgaaaa taattcagag gaatcacagg tttagagtaa atgaaaccac
     4681 aggtaattgg cagtggtaat agggtatggg gtgggaagtt tgggatgatt ttggttagct
     4741 tgagttatcc agttgatcca gacatgataa gatacattga tgagtttgga caaaccacaa
     4801 ctagaatgca gtgaaaaaaa tgctttattt gtgaaattcg taatcatgtc atagctgttt
     4861 cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag
     4921 tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg
     4981 cccgctttcc agtcgggaaa cctgtcgtgc cacggggagc tttttgcaaa agcctaggcc
     5041 tccaaaaaag cctcctcact acttctggaa tagctcagag gccgaggcgg cctcggcctc
     5101 tgcataaata aaaaaaatta gtcagccatg agcttgctgc attaatgaat cggccaacgc
     5161 gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg
     5221 cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta
     5281 tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc
     5341 aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag
     5401 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac
     5461 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc
     5521 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt
     5581 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc
     5641 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga
     5701 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta
     5761 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta
     5821 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga
     5881 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg
     5941 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag
     6001 tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc
     6061 tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact
     6121 tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt
     6181 cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta
     6241 ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta
     6301 tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc
     6361 gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat
     6421 agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt
     6481 atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg
     6541 tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca
     6601 gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta
     6661 agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg
     6721 cgaccgagtt gctcttgccc ggcgtcaaca cgggataata ccgcgccaca tagcagaact
     6781 ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg
     6841 ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt
     6901 actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga
     6961 ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc
     7021 atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa
     7081 caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcta agaaaccatt
     7141 attatcatga cattaaccta taaaaatagg cgtatcacga ggccctttcg tcttcaagaa
     7201 tgatctagcc ctttccttaa ttaacccggg cctctcactc tctgatattc atttctttgc
     7261 aagttataaa tactgaataa taagatgaca tgaactacta ctgctagaga ttttccacac
     7321 tgactaaaag ggtctgaggg atctctagtt accagagtca cacaacagac gggcacacac
     7381 tacttgaagc actcaaggca agctttattg aggcttaagc agtgggttcc ctagttagcc
     7441 agagagctcc caggctcaga tctggtctaa ccagagagac ccagtacaag caaaaagcag
     7501 atcttgtctt cgttgggagt gaattagccc ttccagtccc cccttttctt ttaaaaagtg
     7561 gctaagatct acagctgcct tgtaagtcat tggtcttaaa ggatctcagg cggggaggcg
     7621 gcccaaaggg agatccgact cgtctgaggg cgaaggcgaa gacgcggaag aggccgcaga
     7681 gccggcagca ggccgcggga aggaaggtcc gctggattga gggccgaagg gacgtagcag
     7741 aaggacgtcc cgcgcagaat ccaggtggca acacaggcga gcagccaagg aaaggacgat
     7801 gatttccccg acaacaccac ggaattgtca gtgcccaaca gccgagcccc tgtccagcag
     7861 cgggcaaggc aggcggcgat gagttccgcc gtggcaatag ggagggggaa agcgaaagtc
     7921 ccggaaagga gctgacaggt ggtggcaatg ccccaaccag tgggggttgc gtcagcaaac
     7981 acagtgcaca ccacgccacg ttgcctgaca acgggccaca actcctcata aagagacagc
     8041 aaccaggatt tatacaagga ggagaaaatg aaagccatac gggaagcaat agcatgatac
     8101 aaaggcatta aagcagcgta tccacatagc gtaaaaggag caacatagtt aagaatacca
     8161 gtcaatcttt cacaaatttt gtaatccaga ggttgattag gatccatcga tgcatcaggc
     8221 accgggcttg cgggtcatgc accaggtgcg cggtccttcg ggcacctcga cgtcggcggt
     8281 gacggtgaag ccgagccgct cgtagaaggg gaggttgcgg ggcgcggagg tctccaggaa
     8341 ggcgggcacc ccggcgcgct cggccgcctc cactccgggg agcacgacgg cgctgcccag
     8401 acccttgccc tggtggtcgg gcgagacgcc gacggtggcc aggaaccacg cgggctcctt
     8461 gggccggtgc ggcgccagga ggccttccat ctgttgctgc gcggccagcc gggaaccgct
     8521 caactcggcc atgcgcgggc cgatctcggc gaacaccgcc cccgcttcga cgctctccgg
     8581 cgtggtccag accgccaccg cggcgccgtc gtccgcgacc cacaccttgc cgatgtcgag
     8641 cccgacgcgc gtgaggaaga gttcttgcag ctcggtgacc cgctcgatgt ggcggtccgg
     8701 atcgacggtg tggcgcgtgg cggggtagtc ggcgaacgcg gcggcgaggg tgcgtacggc
     8761 cctggggacg tcgtcgcggg tggcgaggcg caccgtgggc ttgtactcgg tcatggttgt
     8821 ggccatatta tcatcgtgtt tttcaaagga aaaccacgtc cccgtggttc ggggggccta
     8881 gacgtttttt taacctcgac taaacacatg taaagcatgt gcaccgaggc cccagatcag
     8941 atcccataca atggggtacc ttctgggcat ccttcagccc cttgttgaat acgcttgagg
     9001 agagccattt gactctttcc acaactatcc aactcacaac gtggcactgg ggttgtgccg
     9061 cctttgcagg tgtatcttat acacgtggct tttggccgca gaggcacctg tcgccaggtg
     9121 gggggttccg ctgcctgcaa a
//
