LOCUS       sequence-346539-        9678 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector expressing N-terminally tagged 
            eGFP-NDP52/CALCOCO2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9678)
  AUTHORS   Heo JM, Ordureau A, Paulo JA, Rinehart J, Harper JW
  TITLE     The PINK1-PARKIN Mitochondrial Ubiquitylation Pathway Drives a 
            Program of OPTN/NDP52 Recruitment and TBK1 Activation to Promote 
            Mitophagy.
  JOURNAL   Mol Cell. 2015 Oct 1;60(1):7-20. doi: 10.1016/j.molcel.2015.08.016. 
            Epub 2015 Sep 10.
  PUBMED    26365381
REFERENCE   2  (bases 1 to 9678)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2015.08.016"; journalName: "Mol Cell"; date: 
            "2015-10-1- 1"; volume: "60"; issue: "1"; pages: "7-20"
COMMENT     Sequence Label: pHAGE-eGFP-NDP52
FEATURES             Location/Qualifiers
     source          1..9678
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2058..2175
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        2227..2530
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2531..2734
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2685..2705
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2731..2755
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      2814..2823
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(2820..2822,2823..2825,2826..3536)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(2820..2822,2823..2825,2826..3536)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2865..2886)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3126..3145)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3473..3494
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    3552..3576
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(4915..4939)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        4958..5457
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(4993..5016)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     5390..5409
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             5481..5879
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     polyA_signal    5921..6042
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5958..5977)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     misc_feature    6068..6656
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6121..6141)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6539..6550)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6731..6964
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(7083..7101)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7169..7273
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7274..7342,7343..8134)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7492..7511)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8305..8893
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8794..8813
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9047..9064
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9304..9325
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9340..9345,9346..9363,9364..9370)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9378..9394
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9383..9405
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9402..9418
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9402..9418
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg
     2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa
     2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca
     2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactagtgga
     2221 gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg
     2281 cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg
     2341 acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca
     2401 tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc
     2461 ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc
     2521 tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc
     2581 acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa
     2641 tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag
     2701 gcgtgtacgg tgggaggtct atataagcag agctcgttta gtgaaccgtc agatcgcctg
     2761 gagacgccat ccacgctgtt ttgacctcca tagaagacac cggcggccga tctgccacca
     2821 tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc catcctggtc gagctggacg
     2881 gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg
     2941 gcaagctgac cctgaagttc atctgcacca ccggcaagct gcccgtgccc tggcccaccc
     3001 tcgtgaccac cctgacctac ggcgtgcagt gcttcagccg ctaccccgac cacatgaagc
     3061 agcacgactt cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc accatcttct
     3121 tcaaggacga cggcaactac aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg
     3181 tgaaccgcat cgagctgaag ggcatcgact tcaaggagga cggcaacatc ctggggcaca
     3241 agctggagta caactacaac agccacaacg tctatatcat ggccgacaag cagaagaacg
     3301 gcatcaaggt gaacttcaag atccgccaca acatcgagga cggcagcgtg cagctcgccg
     3361 accactacca gcagaacacc cccatcggcg acggccccgt gctgctgccc gacaaccact
     3421 acctgagcac ccagtccgcc ctgagcaaag accccaacga gaagcgcgat cacatggtcc
     3481 tgctggagtt cgtgaccgcc gccgggatca ctctcggcat ggacgagctg tacaaggctc
     3541 gaggttatca aacaagtttg tacaaaaaag ttggcatgga ggagaccatc aaagatcccc
     3601 ccacatcagc tgtcttgctg gatcactgtc atttctctca ggtcatcttt aacagtgtgg
     3661 agaagttcta catccctgga ggggacgtca catgtcatta taccttcacc cagcatttca
     3721 tccctcgtcg aaaggattgg attggcatct ttagagtggg gtggaagaca acccgtgagt
     3781 attacacctt catgtgggtt actttgccca ttgacctaaa caacaaatca gctaaacagc
     3841 aggaagtcca attcaaagct tactacctgc ccaaggatga tgagtattac cagttctgct
     3901 atgtggatga ggatggtgtg gtccggggag caagtattcc tttccaattc cgtccagaaa
     3961 atgaggaaga catcctggtt gttaccactc agggagaggt ggaagagatt gagcagcaca
     4021 acaaggagct ttgcaaagaa aaccaggagc tgaaggacag ctgtatcagc ctccagaagc
     4081 agaactcaga catgcaggct gagctccaaa agaagcagga ggagctagaa accctacaga
     4141 gcatcaataa gaagttggaa ctgaaagtga aagaacagaa ggactattgg gagacagagc
     4201 tgcttcaact gaaagaacaa aaccagaaga tgtcctcaga aaatgagaag atgggaatca
     4261 gagtggatca gcttcaggcc cagctgtcaa ctcaagagaa agaaatggag aagcttgttc
     4321 agggagatca agataagaca gagcagttag agcagctgaa aaaggaaaat gaccacctct
     4381 ttctcagttt aactgaacag aggaaggacc agaagaagct cgagcagaca gtggagcaaa
     4441 tgaagcagaa tgaaactact gcaatgaaga aacaacagga attaatggat gaaaactttg
     4501 acctgtcaaa aagactgagt gagaacgaaa ttatatgtaa tgctctgcag agacagaaag
     4561 agagattgga aggagaaaat gatcttttga agagggagaa cagcagattg ctcagttaca
     4621 tgggtctgga ttttaattct ttgccgtatc aagtacctac ttcagatgaa ggaggcgcaa
     4681 gacaaaatcc aggacttgcc tatggaaacc catattctgg tatccaagaa agttcttccc
     4741 ccagcccgct ctccatcaag aaatgcccta tctgcaaagc agatgatatt tgtgatcaca
     4801 ccttggagca acagcagatg cagccccttt gtttcaattg tccaatttgt gacaagatct
     4861 tcccagctac agagaagcag atctttgaag accacgtgtt ctgccactct ctctgcccaa
     4921 ctttcttgta caaagtggtt cgataacgaa ttctaccggg taggggaggc gcttttccca
     4981 aggcagtctg gagcatgcgc tttagcagcc ccgctgggca cttggcgcta cacaagtggc
     5041 ctctggcctc gcacacattc cacatccacc ggtaggcgcc aaccggctcc gttctttggt
     5101 ggccccttcg cgccaccttc tactcctccc ctagtcagga agttcccccc cgccccgcag
     5161 ctcgcgtcgt gcaggacgtg acaaatggaa gtagcacgtc tcactagtct cgtgcagatg
     5221 gacagcaccg ctgagcaatg gaagcgggta ggcctttggg gcagcggcca atagcagctt
     5281 tgctccttcg ctttctgggc tcagaggctg ggaaggggtg ggtccggggg cgggctcagg
     5341 ggcgggctca ggggcggggc gggcgcccga aggtcctccg gaggcccggc attctgcacg
     5401 cttcaaaagc gcacgtctgc cgcgctgttc tcctcttcct catctccggg cctttcgacc
     5461 tgcagcccat taattaaacc atggccaagc ctttgtctca agaagaatcc accctcattg
     5521 aaagagcaac ggctacaatc aacagcatcc ccatctctga agactacagc gtcgccagcg
     5581 cagctctctc tagcgacggc cgcatcttca ctggtgtcaa tgtatatcat tttactgggg
     5641 gaccttgtgc agaactcgtg gtgctgggca ctgctgctgc tgcggcagct ggcaacctga
     5701 cttgtatcgt cgcgatcgga aatgagaaca ggggcatctt gagcccctgc ggacggtgcc
     5761 gacaggtgct tctcgatctg catcctggga tcaaagccat agtgaaggac agtgatggac
     5821 agccgacggc agttgggatt cgtgaattgc tgccctctgg ttatgtgtgg gagggctaag
     5881 cacttcgtgg ccgaggagca ggactgacac tcgacctcga aacttgttta ttgcagctta
     5941 taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact
     6001 gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct atgcatcgat
     6061 agatcctaat caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta
     6121 tgttgctcct tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc
     6181 ttcccgtatg gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga
     6241 ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac
     6301 ccccactggt tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc
     6361 cctccctatt gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc
     6421 tcggctgttg ggcactgaca attccgtggt gttgtcgggg aaatcatcgt cctttccttg
     6481 gctgctcgcc tgtgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc
     6541 ggccctcaat ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc
     6601 gcgtcttcgc cttcgccctc agacgagtcg gatctccctt tgggccgcct ccccgcctga
     6661 gatcctttaa gaccaatgac ttacaaggca gctgtagatc ttagccactt tttaaaagaa
     6721 aaggggggac tggaagggct aattcactcc caacgaagac aagatctgct ttttgcttgt
     6781 actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac
     6841 ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg
     6901 ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct
     6961 agcagtagta gttcatgtca tcttattatt cagtatttat aacttgcaaa gaaatgaata
     7021 tcagagagtg agaggcccgg gttaattaag gaaagggcta gatcattctt gaagacgaaa
     7081 gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac
     7141 gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat
     7201 acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg
     7261 aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc
     7321 attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga
     7381 tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga
     7441 gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg
     7501 cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc
     7561 tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac
     7621 agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact
     7681 tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca
     7741 tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg
     7801 tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact
     7861 acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg
     7921 accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg
     7981 tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat
     8041 cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc
     8101 tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat
     8161 actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt
     8221 tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc
     8281 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt
     8341 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac
     8401 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt
     8461 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct
     8521 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga
     8581 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac
     8641 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg
     8701 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt
     8761 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc
     8821 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg
     8881 gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc
     8941 ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc
     9001 ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag
     9061 cgaggaagcg gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca
     9121 ttaatgcagc aagctcatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc
     9181 tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc
     9241 aaaaagctcc ccgtggcacg acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc
     9301 aattaatgtg agttagctca ctcattaggc accccaggct ttacacttta tgcttccggc
     9361 tcgtatgttg tgtggaattg tgagcggata acaatttcac acaggaaaca gctatgacat
     9421 gattacgaat ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa
     9481 actcatcaat gtatcttatc atgtctggat caactggata actcaagcta accaaaatca
     9541 tcccaaactt cccaccccat accctattac cactgccaat tacctgtggt ttcatttact
     9601 ctaaacctgt gattcctctg aattattttc attttaaaga aattgtattt gttaaatatg
     9661 tactacaaac ttagtagt
//
