LOCUS       sequence-346547-       12502 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pHIS3H plasmid expressing 13Myc-Vps15 from 41nmt1 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12502)
  AUTHORS   Yu ZQ, Liu XM, Zhao D, Xu DD, Du LL
  TITLE     Visual detection of binary, ternary and quaternary protein 
            interactions in fission yeast using a Pil1 co-tethering assay.
  JOURNAL   J Cell Sci. 2021 Oct 1;134(19):jcs258774. doi: 10.1242/jcs.258774. 
            Epub 2021 Oct 12.
  PUBMED    34499173
REFERENCE   2  (bases 1 to 12502)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Sci."; date: "2021-10-1"; volume: "134(19):jcs258774. doi"; pages: "
            10.1242/jcs.258774. Epub 2021 Oct 12"
COMMENT     Sequence Label: pDB4961
FEATURES             Location/Qualifiers
     source          1..12502
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1233..1730
                     /codon_start=1
                     /product="13 tandem Myc epitope tags"
                     /label=13xMyc
                     /translation="EQKLISEEDLNGEQKLISEEDLNGLDGEQKLISEEDLNGEQKLIS
                     EEDLNGEQKLISEEDLNGLDGEQKLISEEDLNGEQKLISEEDLNGEQKLISEEDLNGLD
                     GEQKLISEEDLNGEQKLISEEDLNGEQKLISEEDLNGLDGEQKLISEEDLNGEQKLISE
                     EDL"
     terminator      6923..7110
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(6978..7002)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     gene            7768..9343
                     /label=hphMX6
                     /note="yeast selectable marker conferring hygromycin 
                     resistance"
     promoter        7768..8111
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
     CDS             8112..9140
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MGKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGR
                     GYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDL
                     PETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHW
                     QTVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAM
                     FGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVD
                     GNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRA
                     KE"
     CDS             8112..9140
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MGKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGR
                     GYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDL
                     PETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHW
                     QTVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAM
                     FGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVD
                     GNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRA
                     KE"
     terminator      9146..9343
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     primer_bind     complement(9397..9414)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(9397..9413)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9406..9428)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      9626..10081
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9713..9732)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9923..9944
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(10098..10117)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     10217..10239
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(10277..10295)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        10363..10467
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(10468..10536,10537..11328)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10686..10705)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      11499..12087
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11988..12007
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     12241..12258
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    12375..12396
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(12411..12416,12417..12434,12435..12441)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12449..12465
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     12454..12476
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     12473..12489
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     12473..12489
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 agcttgcatg cctgcaggtc gatcgactct agaggatcag aaaattatcg ccataaaaga
       61 cagaataagt catcagcggt tgtttcattt cctatatttt ttttttattt ttttattttt
      121 taataaggga aaatttaacg tctaaggata cagaagattg ttagcacatt aaagtaataa
      181 aggcttaagt agtaagtgcc ttagcatgtt attgtatttc aaaggacata atctaaaata
      241 ataacaatat catttctcac aagttattca attttctttt ttttttctaa taatatcaag
      301 aatgtattat ttgtttgaca taagtcaact aatttattta atatgctgga ttaatcttgc
      361 agacatgtaa attaacaagt tttagtcaaa taacgttgaa gtttcaatga actcaaataa
      421 tttctctttt tttttatata accataatct gatttatatt ttccgcaggg atcaactgaa
      481 gttatgacat ttggattgga tcacttataa ccttggtcgc caaataatac aaaaatcagc
      541 gttataaaac aaagaaggtt tttgttaaga aattaatcct ctttcttgat aagaaagttg
      601 aaccgaaatt gcagatactg atatatgaaa ataataccca caattttggg aatagcgcaa
      661 gcctcaattt aaacaatagg tgaggacaca tgataatgac ctcaatgatt gttagaagaa
      721 aagagcctca ttacaaaatc gaaaaatgaa tggttgggta caagtttcca aaacatggta
      781 aagtggactt tgcgtatgag acgtaaatag aaaaaaacac ttgttatatg ttttctagaa
      841 ttattgttgt ctctttatgg ttggatgatg caaaatagta atttcggtta gttgctgtaa
      901 aacaccacga gacaaataga tatggatatt tattaaatca ggaaaaacgt aactctcggc
      961 tactggatgg ttcagtcacc caacgattac tggggagaga aaacagggca aaagcaaagc
     1021 ttaaaggaat ccgattgtca ttcggcaatg tgcagcgaaa ctaaaaaccg gataatggac
     1081 ctgttaatcg aaacattgaa gataaaggaa gaggaatcct ggcatatcat caattgaata
     1141 agttgaatta attatttcaa tctcattctc actttctgac ttatagtcgc tttgttaaag
     1201 ctagcatgcg gatccccggg ttaattaacg gtgaacaaaa gctaatctcc gaggaagact
     1261 tgaacggtga acaaaaatta atctcagaag aagacttgaa cggactcgac ggtgaacaaa
     1321 agttgatttc tgaagaagat ttgaacggtg aacaaaagct aatctccgag gaagacttga
     1381 acggtgaaca aaaattaatc tcagaagaag acttgaacgg actcgacggt gaacaaaagt
     1441 tgatttctga agaagatttg aacggtgaac aaaagctaat ctccgaggaa gacttgaacg
     1501 gtgaacaaaa attaatctca gaagaagact tgaacggact cgacggtgaa caaaagttga
     1561 tttctgaaga agatttgaac ggtgaacaaa agctaatctc cgaggaagac ttgaacggtg
     1621 aacaaaaatt aatctcagaa gaagacttga acggactcga cggtgaacaa aagttgattt
     1681 ctgaagaaga tttgaacggt gaacaaaagc taatctccga ggaagacttg aacggtgaac
     1741 aaaaattaat caatcactac tgtattcgcg ccggcgcgcc aatggggatt cagctttcca
     1801 ctatccagac cccccaattc catgaattgt tcgaggaaga gcttccagaa tatcacaatg
     1861 aaagaagtct aggggattca cattttttgc gaacgtttcg catgcaagat cgcaagggat
     1921 atgatgtttt aatcaaagta tttgtaaata aacttccgga aatttcgttg tcaagcattg
     1981 taaatttgtt gaaggaggag caagagaata tctcatatag agtgccaaat gccgtaccct
     2041 atataaaaac gttggttacg cttcgcgctg cctacctggt gcgtccatac gtgactcaca
     2101 atttgtatga tagaatttct actaggccct ttcttgagtt gactgaaaaa aagtggataa
     2161 tgtttcaatt attaaaggga atttccgatt gtcatagact tggtgtttgt catggtgata
     2221 ttaaatcgga aaacatttta attacttctt ggaattgggc ttatctatcg gactttagct
     2281 cttttaaacc tacttatttg ccagaggata atcctgctga ctacggctat ttttttgaca
     2341 cttcatctcg aagagtctgt aatatcgctc ccgaaagatt tgttcctgca tcgcaattac
     2401 aacctgctcc attatcgcct gcaatggata tttttagtct tggatgtgtg tttgcagaac
     2461 tgcttttgga ggagtcgcct ctttttacgc tttctcagct ttttagctac aaggcccatg
     2521 ggtcttacga tttacagtct gttcttgagc aaatcgagga taagagtacc caaaacatga
     2581 ttctaagtat gctagacaga gatcccagtc aacgattgtc ggctgatgcc tatttgcaga
     2641 aatatcgagg aaccgttttt cctgcatgct tttatgacac tttgtatgat tactgtattg
     2701 gtttagtcga tccaagcgga attctttctg ccaagcagcc taatgacgct tgcagtttgt
     2761 atggttctcg aattgacgac atttacagag atattatgcc cccaaataat aatgacattt
     2821 caaaccttat gcacacccct catgaatata atggaataga ttcttattct gtaccccttt
     2881 ttaccacact tgatgaattt tataataaaa accctgctgc caagaattgg tatctaagac
     2941 tgtataatac tatgcaagaa aaaaaaggat ttgaggataa agagcaaggt tccacacctg
     3001 caccttctcc ttcagtcatt gaggatattt catctattga aacggatgaa aatcatcttt
     3061 atggtgccgc tgttcttctt cccatagttc tttccactat tagacacgtg aatacaagag
     3121 aatctaagat aaacgcttta agtttggtgc aaattttatc aaggaacatc tgcgatgagt
     3181 ccaaacttga tacagtttta ccttttgtta tgactttgct tcgtgaccaa tacgccgatg
     3241 taaggattag tgcgcttatt acgattactc gcctagttag taatgtaacc tctattgctc
     3301 cgattaatgc atttcttttt caggagtatt tatttcctga ccttcagcat tttcttttcg
     3361 atatgaattc tcgtactcga gcaacatatg catcctgcct tccaatttta gccaaacaag
     3421 cttccaaatt tttaaatttg gctcaatcat taaggaatgc tggtatttta tcgtttcctg
     3481 aatccgaata tgaaaatata aatcatggaa aggctgaatt gttatttgaa accggtcgac
     3541 acgatttagt agttactgta gaaaggcacg tttctactct tttagcggac agctcttcta
     3601 ttgttcgtag atctttgctg aatgctttgg ctccgctttg tgtttttttc ggtaaagcaa
     3661 agtctaacga tttgattttg agccatctga taacttactt gaatgacacg gattggatgc
     3721 ttcgttgtgc attttttgaa agtattaccg gtctatccat tttcatcggt ccaaggagtg
     3781 tagatgagta tatacttccc ttgatgctgc aggctttagt agatcctgag ccagctgtcc
     3841 ttgagagtgt cttgggatct ttttctggac tgatcgaatt acacttattt gaaaagcttg
     3901 ttgttgttga tatcttacag cttgtgcttc cacttgttgc tgtacctaat gcttatattc
     3961 gaagagctgc cttgtctgtt atttactctg catatcaaag ttttgatgat attgataggg
     4021 actgtattgt aactccttta ttaagacctt acttgatgtc caatttatgt gatataaact
     4081 ctttagaaaa gctagatcaa tttattttgc ctatggtatc tgatagtgtt tggtctactt
     4141 tgacccgctg gtatgaagaa tccgaaaact caagcttttg gaagtgtgat aaagatgcgt
     4201 cttactcgtc tcttgatgta agcaatgatt cgcttttagg aagaaccaaa aatttgcaaa
     4261 agagagaaat aatgcaccac gcagaagtgt atactcacaa aaaaatctct ctaactggcc
     4321 atgtgattat aacttccaca gaaatgctag agcttagtga agatgaccag aaatgggctg
     4381 acatcattaa agaaatggga gttgatgtaa agcacttatg ggtactggct aatttgagag
     4441 attatgttaa gaaaacgaaa attaatttaa atggaatcaa ctataaacga caaagtggat
     4501 tacgggaact gaatacttac cctaatgctc ccttgcacat attaccagaa accatttttt
     4561 ggaacccaga aagaccccct tcgtcttcta tcgccaatga aactttcttg gacagaaatt
     4621 cttatgcaga atccattcaa acaagccgct cctataacga tgacttaatt gctcgagatc
     4681 ccattgcata tgtagggacg gatatgacta atgcttttgg tacgaccaca aaaccaaaag
     4741 acgtttcaca atcagatatc aaagttgata ttaataggga gtctaattct gataatggag
     4801 agaccattac tggtactcat gatgtataca gacaaacgga caatcctgaa attaaactac
     4861 cttcagacac tgctagctcc aaggttgaca cacacaatcc cacagtcact caaccaacgg
     4921 acgataccgg tgggttgaat tcttataata ctgagaaccc cttactcaca aataatactt
     4981 tggagccttc ctctgtagaa gcaatagtct cttctaaaga ttctgacaaa catgctaaag
     5041 aatcgaaggg gaaaagcctc gcaccgttga tctcttctag ggtttcaacg aatgatacaa
     5101 ctaatgttgc cggtattcga agccaaagat catttactac tcatatcgat ttgaaaaagc
     5161 ttaccacgag gcaaaatggc gcaaaaaaat catcgtatac tggaactaat ccatgcgtct
     5221 taaattattt aaataaaatt tatgcagaag cagcggcttc gacacttaac gttggtgctg
     5281 ctgtttcacc aagctgggct tctaatatac ctttgcgtcg tagaacaaag gtgtctgcga
     5341 aggctgcgaa taaaatagtt cagactcatg aatggcatcc agaaggatca cgagttgctc
     5401 agatatattt aggaagcctt cttgatggag gtacgaagaa agttttggtt agtccagatt
     5461 catctttttt cgttaccttg ggatctgacg gtgttgtaag agcatggcaa ttagttgaaa
     5521 gtgttcgtca tatttcaact atgagatgtg aatgccgatt aagttatggg catacgcgac
     5581 gtaatggcga gaggaataga ttttctgtgg tcaatggatg ctttctaggt aatacatacg
     5641 cttttgcttc tgttactcaa gatggctctg tggaagttca cagacttgac gttaacaatc
     5701 agcggcatac tctaatttcg gctggtcgta ttcccaattt ggatttcagt gattctgtaa
     5761 cctccatgga agcttctaca tttcacgacg ggagtatccg tctagtcgtt gttacaaaat
     5821 ggtcacgcat cgtatactta gacgtgggaa tgatgagggt tttgtcatca gaccaattac
     5881 cattacagtg tgggtcagct actagtgtag tagtttctga gggttgtaat tgggctttaa
     5941 ttgggactac caaaggatgg ctcttacttt gggacctccg ctttgggaca ttatcatgct
     6001 cttggcatat gccagccagg attgatcaga tgcatcttct tttagatgtt accaaaaaac
     6061 gtagcaacgt taatgagtat acttccggaa ataacaatag tcccgttacc aaggttccag
     6121 gtagctcaag tacaagttca agtagtactc agccaattaa ctctactatt cccatcaatc
     6181 ctttagaaaa tcacggaatg ttaaactcgt ttggaagcac tacagtcagt atcagtttta
     6241 gcgtccttac caacttggat aataaagaag ttaatcttga agatgcagtt ccagcgagtc
     6301 acagatccgc ttcaggcata gtaaattttg acgttgaaaa agggaaaacc gaagaggttt
     6361 tcttagaaaa ctggaattct tcactcacca gtccgattcc ggtatctgtt ggaattgatg
     6421 cttttaatga aaaaaaaaag tttgatatag attctggcaa tgacatggga ttaagagatt
     6481 tgaataccaa attcgactct ccttggcctt gtatatctag tccaatctat aggtacagag
     6541 gtccgtctgc aggttctgtc gaacgagagc cactttttct cattgccgct tctggaagcc
     6601 ctcatgcgtt tatttggaat ccccacaatg tctctgctag ttcgtcggtg accaacgact
     6661 ccgagagttc aaaactatct ttaatacata acaaacctcc tatctaccaa aaagtctctg
     6721 aacaacagaa tgtacgccca aagtcttcgg gagtgtcaag gcctcttcta ttccttcaac
     6781 agcaaaagaa tctacctagc gaaaatcgtc tgcaccccat cgttgacatg gccttcttgt
     6841 accaaccgta cgctatagtg cttattgttg acgcttttgg tagtcttgaa ctctggacct
     6901 agggcgcgcc acttctaaat aagcgaattt cttatgattt atgattttta ttattaaata
     6961 agttataaaa aaaataagtg tatacaaatt ttaaagtgac tcttaggttt taaaacgaaa
     7021 attcttattc ttgagtaact ctttcctgta ggtcaggttg ctttctcagg tatagtatga
     7081 ggtcgctctt attgaccaca cctctaccgg cagatccgct agggataaca gggtaatata
     7141 ctagtgttgg cagcgatgaa attattgact ccttaattcg catttcttgt attcctggaa
     7201 aagataagat tttgatgtgt ccaccttcgt acggaatgta tacggtctct gcaaaaatca
     7261 acgatgttga ggttgttaag gttctccttg aaccagattt caatttgaac gtcgatgcta
     7321 tttgcgaaac actttctaaa gatagtgcca tcaaagtctt ttttgcttgt tcccctggta
     7381 atccaacagc ggccgcgggc ccgcggccgc tcttaaactc gaggatatta agaagatttt
     7441 ggagcaccct acatggaatg gaatcgttgt cgtcgatgag gcctatattg atttctctgc
     7501 tcccgatatg tctgccttaa ctcttgtcaa tgaatatcca aaccttgctg tttgccaaac
     7561 cctttcgaaa tctttcggtc tagcaggaat tcggtaagtt aatctaagct tttaagcatt
     7621 aatattattt ctttactaac tatgatgttt agtatcggat tttgtttgac aagtaaacct
     7681 attgctacca ttatgaactc attaaaggct ccttataaca ttagatctgt ttagcttgcc
     7741 tcgtccccgc cgggtcaccc ggccagcgac atggaggccc agaataccct ccttgacagt
     7801 cttgacgtgc gcagctcagg ggcatgatgt gactgtcgcc cgtacattta gcccatacat
     7861 ccccatgtat aatcatttgc atccatacat tttgatggcc gcacggcgcg aagcaaaaat
     7921 tacggctcct cgctgcggac ctgcgagcag ggaaacgctc ccctcacaga cgcgttgaat
     7981 tgtccccacg ccgcgcccct gtagagaaat ataaaaggtt aggatttgcc actgaggttc
     8041 ttctttcata tacttccttt taaaatcttg ctaggataca gttctcacat cacatccgaa
     8101 cataaacaac catgggtaaa aagcctgaac tcaccgcgac gtctgtcgag aagtttctga
     8161 tcgaaaagtt cgacagcgtc tccgacctga tgcagctctc ggagggcgaa gaatctcgtg
     8221 ctttcagctt cgatgtagga gggcgtggat atgtcctgcg ggtaaatagc tgcgccgatg
     8281 gtttctacaa agatcgttat gtttatcggc actttgcatc ggccgcgctc ccgattccgg
     8341 aagtgcttga cattggggaa ttcagcgaga gcctgaccta ttgcatctcc cgccgtgcac
     8401 agggtgtcac gttgcaagac ctgcctgaaa ccgaactgcc cgctgttctg cagccggtcg
     8461 cggaggccat ggatgcgatc gctgcggccg atcttagcca gacgagcggg ttcggcccat
     8521 tcggaccgca aggaatcggt caatacacta catggcgtga tttcatatgc gcgattgctg
     8581 atccccatgt gtatcactgg caaactgtga tggacgacac cgtcagtgcg tccgtcgcgc
     8641 aggctctcga tgagctgatg ctttgggccg aggactgccc cgaagtccgg cacctcgtgc
     8701 acgcggattt cggctccaac aatgtcctga cggacaatgg ccgcataaca gcggtcattg
     8761 actggagcga ggcgatgttc ggggattccc aatacgaggt cgccaacatc ttcttctgga
     8821 ggccgtggtt ggcttgtatg gagcagcaga cgcgctactt cgagcggagg catccggagc
     8881 ttgcaggatc gccgcggctc cgggcgtata tgctccgcat tggtcttgac caactctatc
     8941 agagcttggt tgacggcaat ttcgatgatg cagcttgggc gcagggtcga tgcgacgcaa
     9001 tcgtccgatc cggagccggg actgtcgggc gtacacaaat cgcccgcaga agcgcggccg
     9061 tctggaccga tggctgtgta gaagtactcg ccgatagtgg aaaccgacgc cccagcactc
     9121 gtccgagggc aaaggaataa tcagtactga caataaaaag attcttgttt tcaagaactt
     9181 gtcatttgta tagttttttt atattgtagt tgttctattt taatcaaatg ttagcgtgat
     9241 ttatattttt tttcgcctcg acatcatctg cccagatgcg aagttaagtg cgcagaaagt
     9301 aatatcatgc gtcaatcgta tgtgaatgct ggtcgctata ctgctgtcga ttcgatacta
     9361 acgccgccat ccagtgtcga aaacgagctc gaattcactg gccgtcgttt tacaacgtcg
     9421 tgactgggaa aaccctggcg ttacccaact taatcgcctt gcagcacatc cccctttcgc
     9481 cagctggcgt aatagcgaag aggcccgcac cgatcgccct tcccaacagt tgcgcagcct
     9541 gaatggcgaa tggcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca
     9601 ccgcatacgt caaagcaacc atagtacgcg ccctgtagcg gcgcattaag cgcggcgggt
     9661 gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccttagcgcc cgctcctttc
     9721 gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg
     9781 gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat
     9841 ttgggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg
     9901 ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaactct
     9961 atctcgggct attcttttga tttataaggg attttgccga tttcggtcta ttggttaaaa
    10021 aatgagctga tttaacaaaa atttaacgcg aattttaaca aaatattaac gtttacaatt
    10081 ttatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gccccgacac
    10141 ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga
    10201 caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa
    10261 cgcgcgagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata
    10321 atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt
    10381 ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg
    10441 cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt
    10501 cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta
    10561 aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc
    10621 ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa
    10681 gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc
    10741 cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt
    10801 acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact
    10861 gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac
    10921 aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata
    10981 ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta
    11041 ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg
    11101 gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat
    11161 aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt
    11221 aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga
    11281 aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa
    11341 gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag
    11401 gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac
    11461 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc
    11521 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat
    11581 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat
    11641 actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct
    11701 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt
    11761 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg
    11821 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta
    11881 cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg
    11941 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg
    12001 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc
    12061 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg
    12121 gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat
    12181 aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc
    12241 agcgagtcag tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg
    12301 cgttggccga ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt
    12361 gagcgcaacg caattaatgt gagttagctc actcattagg caccccaggc tttacacttt
    12421 atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca cacaggaaac
    12481 agctatgacc atgattacgc ca
//
