LOCUS       sequence-346558-        4926 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses mScarlet under control of the Capsaspora EF1 promoter and 
            GenR under control of the Capsaspora actin promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4926)
  AUTHORS   Phillips JE, Santos M, Konchwala M, Xing C, Pan D
  TITLE     Genome editing in the unicellular holozoan Capsaspora owczarzaki 
            suggests a premetazoan role for the Hippo pathway in multicellular 
            morphogenesis.
  JOURNAL   Elife. 2022 Jun 6;11. pii: 77598. doi: 10.7554/eLife.77598.
  PUBMED    35659869
REFERENCE   2  (bases 1 to 4926)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2022-06-6"; volume: "11. pii: 77598. doi"; pages: " 
            10.7554/eLife.77598"
COMMENT     Sequence Label: pJP72
FEATURES             Location/Qualifiers
     source          1..4926
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1788..1892
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1893..1961,1962..2753)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2111..2130)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2924..3512
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3413..3432
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gtgttgaaga gggaaaaaaa cgaaaaaaga aaaaagtgaa aaaaaggcaa ttttttgttg
       61 ttttccagaa cgttctaaat ttcaaaacag aaacggcttg tccgatgcga tcgatcgtga
      121 cagcgcagca gtggcagacg attgcaagac gagcatctca cgcaagaggc aaaagagcgc
      181 gaagaagaga gcggagaaga ggaagcgaga gagaaaagcc cgaaggcgaa aaagaaaaag
      241 aaaaaaaaaa gaaccacatg tttctgtttc gcgtttttcg ttttccactt tcacgtccat
      301 ctccaggccg aggaaagggg catcggatgg cccgcgcacc gcaatccagt gtgcagagac
      361 actgtctagc ggattgcgag gaggggagga ggggacaagc ggggatgggc cgagatcggc
      421 ttcgagaagg aagatggaag caagtcacgt tcgatttttt ttcttttcac aaacctgtaa
      481 aagagtgctc tctcccaaat tcggttgtcg ttctatttca aaatgcagca tcaaaagcta
      541 aatcgacaac gattggggga aaaaaactga gagtttggag gttgctgatt gtcggtcgtt
      601 gctaactgtg catttgccgg cccctttttt ctttgtacaa gatcacgcag caacagacac
      661 aatccgcatc aggcagcgtt ggcgactagg gcgctcggag agaacgcgct tagaagaact
      721 cgtcgaggag gcggtagaag gcgatgcgct gcgaatcggg agcggcgata ccgtagagga
      781 cgaggaagcg gtcagcccat tcgccgccga gctcttcagc aatatcacgg gtagcgaggg
      841 cgatgtcctg gtagcggtcg gcgacaccca gacggccgca gtcgatgaag cccgaaaagc
      901 ggccgttttc gaccatgatg ttggggaggc aggcatcgcc gtgggtgacg acgagatcct
      961 cgccgtcggg catgcgggcc ttgaggcggg cgaacagttc ggcgggggcg aggccctggt
     1021 gctcttcgtc cagatcatcc tgatcgacga gaccggcttc catgcgagta cgggcgcgct
     1081 cgatgcggtg cttggcttgg tggtcgaagg ggcaggtagc gggatcgagg gtgtgcaggc
     1141 ggcgcatggc atcagccatg atggagacct tctcggcggg agcgaggtgc gacgacagga
     1201 gatcctggcc ggggacttcg ccgaggagca gccagtcgcg gccggcttca gtgacaacgt
     1261 cgaggacagc ggcgcaggga acgccggtgg tggccagcca cgagaggcgg gcggcctcgt
     1321 cctgcagttc gttcagggca ccggacaggt cggtcttgac aaagagaacg gggcggccct
     1381 gggccgacag gcggaagacg gcggcatccg agcagccgat ggtctgttgg gcccagtcgt
     1441 agccgaagag gcgctcgacc caagcggcgg gcgaaccggc gtggaggcca tcttgttcaa
     1501 tcatgcgaaa cgagccagct tgaacatctt caccatccat tttggtgggt tggtgggatt
     1561 taactaggtt aaaagactga tctgaaactc gtccaaaacg ggccgcctta aataccccca
     1621 gcgggtcgca gcacgatttc aggtcagtct gccatggcag tttgacctga aaaacctgtt
     1681 tgttgactgc ggtttccaaa gatggcaagc cgcagtggaa aaaaacaaac aatcagcatt
     1741 tttgtgggat cattgggacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta
     1801 tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat
     1861 aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc
     1921 ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga
     1981 aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca
     2041 acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt
     2101 ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg
     2161 gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc
     2221 atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata
     2281 acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt
     2341 tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag
     2401 ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca
     2461 aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg
     2521 aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg
     2581 ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag
     2641 atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg
     2701 aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag
     2761 accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga
     2821 tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt
     2881 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc
     2941 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc
     3001 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac
     3061 caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac
     3121 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt
     3181 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct
     3241 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat
     3301 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt
     3361 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg
     3421 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt
     3481 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt
     3541 tcctggcctt ttgctggcct tttgctcaca tgttctttcc gatgccggcg gtgagaacag
     3601 cactagcaat gagcaatcac acgcatctag gtgccaggcg cagcatgatt gaaaatggca
     3661 acactttcgc cgcacaaagt caaaccattt cattccactt gttctccatt tccccattcc
     3721 ttgctcatcc ctccactgta aaaacaactc gtcgtgtttc ttgccataat ggaatatatt
     3781 gcgctcataa ccaacgcaaa aaaaatgaca accaaaaaaa acttcaatgc aagcaacaaa
     3841 aatcatgaaa tccctttttg gtcttagaaa tgattgaaat aaacaaatgt gtcttggcaa
     3901 acacaaaatg cggccgcctt aagacttctt gtacagctcg tccatgccgc cggtggagtg
     3961 gcggccctcg gagcgttcgt actgttcgac gacggtgtag tcctcgttgt gggaggtgat
     4021 gtcgagctta cggtcgacgt tgtaggcacc gggcatctgg acgggcttct tggccttgta
     4081 ggtggtcttg aagtcggcca ggtagcggcc gccgtccttc aggcgcagag ccatcttaat
     4141 gtcgcccttc aggacaccgt cctcggggta gagacgctcg gtggaggctt cccagcccat
     4201 ggtcttcttc tgcatgacgg ggccgtcggg ggggaagttg gtgccgcgga gcttgacctt
     4261 gtagatcagg gtgccgtcct ccagggaggt gtcctgggtg acggtgacgg caccgccgtc
     4321 ctcgaagttc atgacgcgct cccacttgaa gccctcgggg aaggactgct tgtagtagtc
     4381 ggggatgtcg gcggggtgct tggtgaaagc acgggagccg tacatgaact ggggggacag
     4441 gatgtcccag gagaagggca ggggaccacc cttggtgacc ttcagcttgg cggtctgggt
     4501 gccctcgtag gggcggccct cgccctcacc ctcgatctcg aactcgtggc cgttcatgga
     4561 gccctccatg tggaccttga agcgcatgaa ctccttgatg acggcctcgc ccttcgagac
     4621 catggtacca ctagttgtga aggttgttct gtgtgtgtta aataataaaa aaatggaaga
     4681 gtgagcattt ccaaatcaaa aagcgacaat tatttaaatc aggattgcac gtggggagaa
     4741 ggaagaaaac gatgaaacag cattttaaaa aaaaacactc gtggggttcg gcgtcaacac
     4801 cccacgctga cgttcgcgtt tcgacgctcg tttttggcaa tgcgcaaggg gatggggatg
     4861 aatggatcct aatccgagtg tgcacgacga gagcgaagaa caaacgcaga acgacagttg
     4921 gaggaa
//
