LOCUS       sequence-346559-        5133 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses mScarlet under control of the Capsaspora EF1 promoter and 
            HygR under control of the Capsaspora actin promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5133)
  AUTHORS   Phillips JE, Santos M, Konchwala M, Xing C, Pan D
  TITLE     Genome editing in the unicellular holozoan Capsaspora owczarzaki 
            suggests a premetazoan role for the Hippo pathway in multicellular 
            morphogenesis.
  JOURNAL   Elife. 2022 Jun 6;11. pii: 77598. doi: 10.7554/eLife.77598.
  PUBMED    35659869
REFERENCE   2  (bases 1 to 5133)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2022-06-6"; volume: "11. pii: 77598. doi"; pages: " 
            10.7554/eLife.77598"
COMMENT     Sequence Label: pJP103
FEATURES             Location/Qualifiers
     source          1..5133
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1937..2041
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2042..2110,2111..2902)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2260..2279)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3073..3661
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3562..3581
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tgttttccag aacgttctaa atttcaaaac agaaacggct tgtccgatgc gatcgatcgt
       61 gacagcgcag cagtggcaga cgattgcaag acgagcatct cacgcaagag gcaaaagagc
      121 gcgaagaaga gagcggagaa gaggaagcga gagagaaaag cccgaaggcg aaaaagaaaa
      181 agaaaaaaaa aagaaccaca tgtttctgtt tcgcgttttt cgttttccac tttcacgtcc
      241 atctccaggc cgaggaaagg ggcatcggat ggcccgcgca ccgcaatcca gtgtgcagag
      301 acactgtcta gcggattgcg aggaggggag gaggggacaa gcggggatgg gccgagatcg
      361 gcttcgagaa ggaagatgga agcaagtcac gttcgatttt ttttcttttc acaaacctgt
      421 aaaagagtgc tctctcccaa attcggttgt cgttctattt caaaatgcag catcaaaagc
      481 taaatcgaca acgattgggg gaaaaaaact gagagtttgg aggttgctga ttgtcggtcg
      541 ttgctaactg tgcatttgcc ggcccctttt ttctttgtac aagatcacgc agcaacagac
      601 acaatccgca tcaggcagcg ttggcgacta gggcgctcgg agagaacgcg cttagttagc
      661 ctcgcccatt tcgcgatctg gacgagtcga ggggcggcgg ttacccgaat cggcgaggac
      721 ttcgacacaa ccatcggtcc agacggcagc cgagcggcgg gcgatttgag tacggccgac
      781 agtgccggcg ccggagcgga cgatggcgtc acaacgaccc tgagcccaag cagcatcatc
      841 gaagttgccg tcgacgagcg actggtagag ctggtcgaga ccaatgcgga gcatgtaggc
      901 acgaaggcgg ggcgagccgg caagttcggg gtggcgacgc tcgaagtagc gggtctgctg
      961 ttccatgcaa gcgagccagg ggcgccagaa aaaaatgttg gcgacctcgt actgggaatc
     1021 gccgaacatg gcctccgacc agtcaatgac ggcggtgatg cgaccgttgt cggtcagaac
     1081 gttattagag ccgaaatcgg cgtggacaag gtggcggact tcgggacagt cctcggccca
     1141 gagcatcagc tcatcgagag cctgggcaac ggaagccgag acggtgtcgt ccatgacagt
     1201 ctgccagtgg taaacgtggg gatcagcaat agcacagatg aaatcacgcc aggtagtgta
     1261 ctgaccaatg ccctgggggc cgaaggggcc gaagcccgag gtctgcgaga gatcggcggc
     1321 agcaatggca tccatggcct cagcgacggg ttgcagaaca gcgggcagtt cggtttcggg
     1381 caggtcctgg agggtgacac cctgggcacg gcgggagatg cagtaggtca gcgactccga
     1441 aaattcgcca atgtcgagga cttcgggaat tgggagggcg gccgaggcaa agtggcggta
     1501 gacgtaacga tccttgtaga aaccatcggc gcacgagttg acgcgcagga cgtagccacg
     1561 gccgccgaca tcgaacgaga aagcacgcga ttcttcgccc tccgagagct gcatcaggtc
     1621 ggagaccgag tcgaactttt cgatcagaaa cttctcgacc gaggtggcgg tgagttcggg
     1681 cttcttcatt ttggtgggtt ggtgggattt aactaggtta aaagactgat ctgaaactcg
     1741 tccaaaacgg gccgccttaa atacccccag cgggtcgcag cacgatttca ggtcagtctg
     1801 ccatggcagt ttgacctgaa aaacctgttt gttgactgcg gtttccaaag atggcaagcc
     1861 gcagtggaaa aaaacaaaca atcagcattt ttgtgggatc attgggacgt caggtggcac
     1921 ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat
     1981 gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag
     2041 tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc
     2101 tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc
     2161 acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc
     2221 cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc
     2281 ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt
     2341 ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt
     2401 atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat
     2461 cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct
     2521 tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat
     2581 gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc
     2641 ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg
     2701 ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc
     2761 tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta
     2821 cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc
     2881 ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga
     2941 tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat
     3001 gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat
     3061 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa
     3121 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa
     3181 ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt
     3241 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
     3301 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata
     3361 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt
     3421 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac
     3481 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga
     3541 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
     3601 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa
     3661 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat
     3721 gttctttccg atgccggcgg tgagaacagc actagcaatg agcaatcaca cgcatctagg
     3781 tgccaggcgc agcatgattg aaaatggcaa cactttcgcc gcacaaagtc aaaccatttc
     3841 attccacttg ttctccattt ccccattcct tgctcatccc tccactgtaa aaacaactcg
     3901 tcgtgtttct tgccataatg gaatatattg cgctcataac caacgcaaaa aaaatgacaa
     3961 ccaaaaaaaa cttcaatgca agcaacaaaa atcatgaaat ccctttttgg tcttagaaat
     4021 gattgaaata aacaaatgtg tcttggcaaa cacaaaatgc ggccgcctta agacttcttg
     4081 tacagctcgt ccatgccgcc ggtggagtgg cggccctcgg agcgttcgta ctgttcgacg
     4141 acggtgtagt cctcgttgtg ggaggtgatg tcgagcttac ggtcgacgtt gtaggcaccg
     4201 ggcatctgga cgggcttctt ggccttgtag gtggtcttga agtcggccag gtagcggccg
     4261 ccgtccttca ggcgcagagc catcttaatg tcgcccttca ggacaccgtc ctcggggtag
     4321 agacgctcgg tggaggcttc ccagcccatg gtcttcttct gcatgacggg gccgtcgggg
     4381 gggaagttgg tgccgcggag cttgaccttg tagatcaggg tgccgtcctc cagggaggtg
     4441 tcctgggtga cggtgacggc accgccgtcc tcgaagttca tgacgcgctc ccacttgaag
     4501 ccctcgggga aggactgctt gtagtagtcg gggatgtcgg cggggtgctt ggtgaaagca
     4561 cgggagccgt acatgaactg gggggacagg atgtcccagg agaagggcag gggaccaccc
     4621 ttggtgacct tcagcttggc ggtctgggtg ccctcgtagg ggcggccctc gccctcaccc
     4681 tcgatctcga actcgtggcc gttcatggag ccctccatgt ggaccttgaa gcgcatgaac
     4741 tccttgatga cggcctcgcc cttcgagacc atggtaccac tagttgtgaa ggttgttctg
     4801 tgtgtgttaa ataataaaaa aatggaagag tgagcatttc caaatcaaaa agcgacaatt
     4861 atttaaatca ggattgcacg tggggagaag gaagaaaacg atgaaacagc attttaaaaa
     4921 aaaacactcg tggggttcgg cgtcaacacc ccacgctgac gttcgcgttt cgacgctcgt
     4981 ttttggcaat gcgcaagggg atggggatga atggatccta atccgagtgt gcacgacgag
     5041 agcgaagaac aaacgcagaa cgacagttgg aggaagtgtt gaagagggaa aaaaacgaaa
     5101 aaagaaaaaa gtgaaaaaaa ggcaattttt tgt
//
