LOCUS       sequence-346560-        2225 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  yeast MoClo level-0 part plasmid type 8b containing 5' homology for 
            integration site X-4.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2225)
  AUTHORS   Otto M, Skrekas C, Gossing M, Gustafsson J, Siewers V, David F
  TITLE     Expansion of the Yeast Modular Cloning Toolkit for CRISPR-Based 
            Applications, Genomic Integrations and Combinatorial Libraries.
  JOURNAL   ACS Synth Biol. 2021 Dec 17;10(12):3461-3474. doi: 
            10.1021/acssynbio.1c00408. Epub 2021 Dec 3.
  PUBMED    34860007
REFERENCE   2  (bases 1 to 2225)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.1c00408"; journalName: "ACS Synth Biol"; date: 
            "2021-12-17- 17"; volume: "10"; issue: "12"; pages: "3461-3474"
COMMENT     Sequence Label: pMC8b-X4up
FEATURES             Location/Qualifiers
     source          1..2225
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(328..362)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(433..1092)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1007..1026
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      1274..1862
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1763..1782
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gaagatgtcc tttgtcaagg gaggcacaga gcatggccaa tttggcaaat tgcaggtttt
       61 tctgagtgaa aaatgaaaaa gcattgtagt agagtcggct cactgaaaaa ccggggagga
      121 cgaaaaggtt tccagccaca gttgtagtca cgtgcgcgcc atgctgacta atggcagccg
      181 tcgttgggca gaagagaatt agtatggtac aggatacgct aattgcgctc caactaccaa
      241 ggttgttgag ggaacactgg ggcaataggc tgtcgccatt caagagcaga ttaagtcctc
      301 agcgagctcg cccttgagac cagaccaata aaaaacgccc ggcggcaacc gagcgttctg
      361 aacaaatcca gatggagttc tgaggtcatt actggatcta tcaacaggag tccaagcgag
      421 ctcgatatca aattacgccc cgccctgcca ctcatcgcag tactgttgta attcattaag
      481 cattctgccg acatggaagc catcacaaac ggcatgatga acctgaatcg ccagcggcat
      541 cagcaccttg tcgccttgcg tataatattt gcccatggtg aaaacggggg cgaagaagtt
      601 gtccatattg gccacgttta aatcaaaact ggtgaaactc acccagggat tggctgaaac
      661 gaaaaacata ttctcaataa accctttagg gaaataggcc aggttttcac cgtaacacgc
      721 cacatcttgc gaatatatgt gtagaaactg ccggaaatcg tcgtggtatt cactccagag
      781 cgatgaaaac gtttcagttt gctcatggaa aacggtgtaa caagggtgaa cactatccca
      841 tatcaccagc tcaccgtctt tcattgccat acgaaattcc ggatgagcat tcatcaggcg
      901 ggcaagaatg tgaataaagg ccggataaaa cttgtgctta tttttcttta cggtctttaa
      961 aaaggccgta atatccagct gaacggtctg gttataggta cattgagcaa ctgactgaaa
     1021 tgcctcaaaa tgttctttac gatgccattg ggatatatca acggtggtat atccagtgat
     1081 ttttttctcc attttagctt ccttagctcc tgaaaatctc gataactcaa aaaatacgcc
     1141 cggtagtgat cttatttcat tatggtgaaa gttggaacct cttacgtgcc cgatcaatca
     1201 tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga
     1261 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
     1321 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
     1381 aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt
     1441 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
     1501 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
     1561 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
     1621 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
     1681 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
     1741 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
     1801 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
     1861 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
     1921 tgttctttcc tgcgttatcc cctgattctg tggataaccg tagtcggtct cacaatgcgc
     1981 tgagggttta atcccaaagc taagagtccc attttattct tctatatgta tattttcgat
     2041 actctaaacc accctacaat gtagccctat actaaatctg ctcaattttc agcttctaca
     2101 agtgactcga ggccacgtgg aaagatccaa ctactccagc acaacgattc aatataatcg
     2161 attgctccac tcataagagg caagaacaag cttcaacttt tggtaagccg ccgtttataa
     2221 acagg
//
