LOCUS       sequence-346561-        2258 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  yeast MoClo level-0 part plasmid type 7 containing 3' homology for 
            integration site XII-2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2258)
  AUTHORS   Otto M, Skrekas C, Gossing M, Gustafsson J, Siewers V, David F
  TITLE     Expansion of the Yeast Modular Cloning Toolkit for CRISPR-Based 
            Applications, Genomic Integrations and Combinatorial Libraries.
  JOURNAL   ACS Synth Biol. 2021 Dec 17;10(12):3461-3474. doi: 
            10.1021/acssynbio.1c00408. Epub 2021 Dec 3.
  PUBMED    34860007
REFERENCE   2  (bases 1 to 2258)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.1c00408"; journalName: "ACS Synth Biol"; date: 
            "2021-12-17- 17"; volume: "10"; issue: "12"; pages: "3461-3474"
COMMENT     Sequence Label: pMC7-XII2dw
FEATURES             Location/Qualifiers
     source          1..2258
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     371..390
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1194..1228)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(1299..1958)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1873..1892
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      join(2140..2258,1..470)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact
       61 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg
      121 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata
      181 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga
      241 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag
      301 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg
      361 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac
      421 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
      481 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg
      541 cgttatcccc tgattctgtg gataaccgta gtcggtctca gagtcctgca ggactagtgc
      601 tgaggcatta atcgactctc tcgaaatttt tcttaacgcg tccttgtact gcgtctaacg
      661 cttttgccac ttggatttct attataggaa atagtctcac ttactgggcg acgaattttc
      721 gcgttttgat gaagcacagg aagaatttct tttttttttg gcttcttctg gttccgtttt
      781 ttacgcgcac aaatctaaaa aaagaaataa ttataaccta gtctcgaaaa ttttcatcga
      841 tccattcgtt cctttttttc gattttttca gatcaaaatt cttgtttctt tctttgtctt
      901 agtttatatt aaaagatatt ttgattttac tcctgaacta tttattcttt ctaagaaggc
      961 cagaacacta cagctgtttt aaccgactac gaagttctcc attctcgaac actagccttc
     1021 atttaccaaa caggaactag cgtatatcat tagtccttat tcgaaaagag attggtagat
     1081 atttattgta gtttgtgaga aggagaaaat actgtcattg gactgatagt tagaggacat
     1141 taacctctct tacgttcgct cattaagacc tcagcgccga tgagaccaga ccaataaaaa
     1201 acgcccggcg gcaaccgagc gttctgaaca aatccagatg gagttctgag gtcattactg
     1261 gatctatcaa caggagtcca agcgagctcg atatcaaatt acgccccgcc ctgccactca
     1321 tcgcagtact gttgtaattc attaagcatt ctgccgacat ggaagccatc acaaacggca
     1381 tgatgaacct gaatcgccag cggcatcagc accttgtcgc cttgcgtata atatttgccc
     1441 atggtgaaaa cgggggcgaa gaagttgtcc atattggcca cgtttaaatc aaaactggtg
     1501 aaactcaccc agggattggc tgaaacgaaa aacatattct caataaaccc tttagggaaa
     1561 taggccaggt tttcaccgta acacgccaca tcttgcgaat atatgtgtag aaactgccgg
     1621 aaatcgtcgt ggtattcact ccagagcgat gaaaacgttt cagtttgctc atggaaaacg
     1681 gtgtaacaag ggtgaacact atcccatatc accagctcac cgtctttcat tgccatacga
     1741 aattccggat gagcattcat caggcgggca agaatgtgaa taaaggccgg ataaaacttg
     1801 tgcttatttt tctttacggt ctttaaaaag gccgtaatat ccagctgaac ggtctggtta
     1861 taggtacatt gagcaactga ctgaaatgcc tcaaaatgtt ctttacgatg ccattgggat
     1921 atatcaacgg tggtatatcc agtgattttt ttctccattt tagcttcctt agctcctgaa
     1981 aatctcgata actcaaaaaa tacgcccggt agtgatctta tttcattatg gtgaaagttg
     2041 gaacctctta cgtgcccgat caatcatgac caaaatccct taacgtgagt tttcgttcca
     2101 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
     2161 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
     2221 tcaagagcta ccaactcttt ttccgaaggt aactggct
//
