LOCUS       sequence-346562-        4668 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses mScarlet under control of the Capsaspora EF1 promoter and 
            NatR under control of the Capsaspora actin promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4668)
  AUTHORS   Phillips JE, Santos M, Konchwala M, Xing C, Pan D
  TITLE     Genome editing in the unicellular holozoan Capsaspora owczarzaki 
            suggests a premetazoan role for the Hippo pathway in multicellular 
            morphogenesis.
  JOURNAL   Elife. 2022 Jun 6;11. pii: 77598. doi: 10.7554/eLife.77598.
  PUBMED    35659869
REFERENCE   2  (bases 1 to 4668)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2022-06-6"; volume: "11. pii: 77598. doi"; pages: " 
            10.7554/eLife.77598"
COMMENT     Sequence Label: pJP102
FEATURES             Location/Qualifiers
     source          1..4668
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        803..907
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(908..976,977..1768)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1126..1145)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1939..2527
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2428..2447
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 catgtaaagg gcctgctcgc cgtccgaggc ggtgccgtcg tacagggcgg tgtccaggcc
       61 gcagagggtg aagcccatac ggcggtaggc gtggatggcg ggggcgttga cgttggtgac
      121 ctccagccag aggtggccgg cgccgcgctc acgagcgaac tcggtggcga ggcccatgag
      181 agcgcggcca acgccgtggc cgcggtgctc gggggcaacc tcgatgtcct cgacggtcag
      241 gcggcggttc caaccggaat aagagacgac gacgaagccg gccaggtcgc cgtcgtcgcc
      301 gtaggcgaca aaggtgcggg agtcggggtc gccgtcctcg ccgtcgtcag attcgtcgtc
      361 cgattcgtcg tcgggaaaga ccttggtcag gggggggtca acggggacct cacgcagggt
      421 aaagccgtcg ccggtggcag tgacgcggaa aacggtgtcg gtggtgaaag agccatccag
      481 agcctcgatg gcttcggcgt cgccggggac cgaggtgcgg taacggtaag cggtgtcgtc
      541 gagagtggta cccattttgg tgggttggtg ggatttaact aggttaaaag actgatctga
      601 aactcgtcca aaacgggccg ccttaaatac ccccagcggg tcgcagcacg atttcaggtc
      661 agtctgccat ggcagtttga cctgaaaaac ctgtttgttg actgcggttt ccaaagatgg
      721 caagccgcag tggaaaaaaa caaacaatca gcatttttgt gggatcattg ggacgtcagg
      781 tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc
      841 aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag
      901 gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg
      961 ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt
     1021 gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt
     1081 tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt
     1141 attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa
     1201 tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag
     1261 agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac
     1321 aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac
     1381 tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac
     1441 cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac
     1501 tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact
     1561 tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg
     1621 tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt
     1681 tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat
     1741 aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta
     1801 gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa
     1861 tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga
     1921 aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac
     1981 aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt
     2041 tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc
     2101 gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat
     2161 cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag
     2221 acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc
     2281 cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag
     2341 cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac
     2401 aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg
     2461 gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct
     2521 atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc
     2581 tcacatgttc tttccgatgc cggcggtgag aacagcacta gcaatgagca atcacacgca
     2641 tctaggtgcc aggcgcagca tgattgaaaa tggcaacact ttcgccgcac aaagtcaaac
     2701 catttcattc cacttgttct ccatttcccc attccttgct catccctcca ctgtaaaaac
     2761 aactcgtcgt gtttcttgcc ataatggaat atattgcgct cataaccaac gcaaaaaaaa
     2821 tgacaaccaa aaaaaacttc aatgcaagca acaaaaatca tgaaatccct ttttggtctt
     2881 agaaatgatt gaaataaaca aatgtgtctt ggcaaacaca aaatgcggcc gccttaagac
     2941 ttcttgtaca gctcgtccat gccgccggtg gagtggcggc cctcggagcg ttcgtactgt
     3001 tcgacgacgg tgtagtcctc gttgtgggag gtgatgtcga gcttacggtc gacgttgtag
     3061 gcaccgggca tctggacggg cttcttggcc ttgtaggtgg tcttgaagtc ggccaggtag
     3121 cggccgccgt ccttcaggcg cagagccatc ttaatgtcgc ccttcaggac accgtcctcg
     3181 gggtagagac gctcggtgga ggcttcccag cccatggtct tcttctgcat gacggggccg
     3241 tcggggggga agttggtgcc gcggagcttg accttgtaga tcagggtgcc gtcctccagg
     3301 gaggtgtcct gggtgacggt gacggcaccg ccgtcctcga agttcatgac gcgctcccac
     3361 ttgaagccct cggggaagga ctgcttgtag tagtcgggga tgtcggcggg gtgcttggtg
     3421 aaagcacggg agccgtacat gaactggggg gacaggatgt cccaggagaa gggcagggga
     3481 ccacccttgg tgaccttcag cttggcggtc tgggtgccct cgtaggggcg gccctcgccc
     3541 tcaccctcga tctcgaactc gtggccgttc atggagccct ccatgtggac cttgaagcgc
     3601 atgaactcct tgatgacggc ctcgcccttc gagaccatgg taccactagt tgtgaaggtt
     3661 gttctgtgtg tgttaaataa taaaaaaatg gaagagtgag catttccaaa tcaaaaagcg
     3721 acaattattt aaatcaggat tgcacgtggg gagaaggaag aaaacgatga aacagcattt
     3781 taaaaaaaaa cactcgtggg gttcggcgtc aacaccccac gctgacgttc gcgtttcgac
     3841 gctcgttttt ggcaatgcgc aaggggatgg ggatgaatgg atcctaatcc gagtgtgcac
     3901 gacgagagcg aagaacaaac gcagaacgac agttggagga agtgttgaag agggaaaaaa
     3961 acgaaaaaag aaaaaagtga aaaaaaggca attttttgtt gttttccaga acgttctaaa
     4021 tttcaaaaca gaaacggctt gtccgatgcg atcgatcgtg acagcgcagc agtggcagac
     4081 gattgcaaga cgagcatctc acgcaagagg caaaagagcg cgaagaagag agcggagaag
     4141 aggaagcgag agagaaaagc ccgaaggcga aaaagaaaaa gaaaaaaaaa agaaccacat
     4201 gtttctgttt cgcgtttttc gttttccact ttcacgtcca tctccaggcc gaggaaaggg
     4261 gcatcggatg gcccgcgcac cgcaatccag tgtgcagaga cactgtctag cggattgcga
     4321 ggaggggagg aggggacaag cggggatggg ccgagatcgg cttcgagaag gaagatggaa
     4381 gcaagtcacg ttcgattttt tttcttttca caaacctgta aaagagtgct ctctcccaaa
     4441 ttcggttgtc gttctatttc aaaatgcagc atcaaaagct aaatcgacaa cgattggggg
     4501 aaaaaaactg agagtttgga ggttgctgat tgtcggtcgt tgctaactgt gcatttgccg
     4561 gccccttttt tctttgtaca agatcacgca gcaacagaca caatccgcat caggcagcgt
     4621 tggcgactag ggcgctcgga gagaacgcgc ttagggacag ggcatcga
//
