LOCUS       sequence-346563-        5210 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses Lifeact-mScarlet under control of the Capsaspora EF1 
            promoter and HygR under control of the Capsaspora actin promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5210)
  AUTHORS   Phillips JE, Santos M, Konchwala M, Xing C, Pan D
  TITLE     Genome editing in the unicellular holozoan Capsaspora owczarzaki 
            suggests a premetazoan role for the Hippo pathway in multicellular 
            morphogenesis.
  JOURNAL   Elife. 2022 Jun 6;11. pii: 77598. doi: 10.7554/eLife.77598.
  PUBMED    35659869
REFERENCE   2  (bases 1 to 5210)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2022-06-6"; volume: "11. pii: 77598. doi"; pages: " 
            10.7554/eLife.77598"
COMMENT     Sequence Label: pJP118
FEATURES             Location/Qualifiers
     source          1..5210
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1924..2028
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2029..2097,2098..2889)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2247..2266)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3060..3648
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3549..3568
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(4787..4837)
                     /codon_start=1
                     /product="peptide that binds filamentous actin (Riedl et 
                     al., 2008)"
                     /label=Lifeact
                     /note="first 17 amino acids of budding yeast Abp140"
                     /translation="MGVADLIKKFESISKEE"
ORIGIN
        1 gttctaaatt tcaaaacaga aacggcttgt ccgatgcgat cgatcgtgac agcgcagcag
       61 tggcagacga ttgcaagacg agcatctcac gcaagaggca aaagagcgcg aagaagagag
      121 cggagaagag gaagcgagag agaaaagccc gaaggcgaaa aagaaaaaga aaaaaaaaag
      181 aaccacatgt ttctgtttcg cgtttttcgt tttccacttt cacgtccatc tccaggccga
      241 ggaaaggggc atcggatggc ccgcgcaccg caatccagtg tgcagagaca ctgtctagcg
      301 gattgcgagg aggggaggag gggacaagcg gggatgggcc gagatcggct tcgagaagga
      361 agatggaagc aagtcacgtt cgattttttt tcttttcaca aacctgtaaa agagtgctct
      421 ctcccaaatt cggttgtcgt tctatttcaa aatgcagcat caaaagctaa atcgacaacg
      481 attgggggaa aaaaactgag agtttggagg ttgctgattg tcggtcgttg ctaactgtgc
      541 atttgccggc cccttttttc tttgtacaag atcacgcagc aacagacaca atccgcatca
      601 ggcagcgttg gcgactaggg cgctcggaga gaacgcgctt agttagcctc gcccatttcg
      661 cgatctggac gagtcgaggg gcggcggtta cccgaatcgg cgaggacttc gacacaacca
      721 tcggtccaga cggcagccga gcggcgggcg atttgagtac ggccgacagt gccggcgccg
      781 gagcggacga tggcgtcaca acgaccctga gcccaagcag catcatcgaa gttgccgtcg
      841 acgagcgact ggtagagctg gtcgagacca atgcggagca tgtaggcacg aaggcggggc
      901 gagccggcaa gttcggggtg gcgacgctcg aagtagcggg tctgctgttc catgcaagcg
      961 agccaggggc gccagaaaaa aatgttggcg acctcgtact gggaatcgcc gaacatggcc
     1021 tccgaccagt caatgacggc ggtgatgcga ccgttgtcgg tcagaacgtt attagagccg
     1081 aaatcggcgt ggacaaggtg gcggacttcg ggacagtcct cggcccagag catcagctca
     1141 tcgagagcct gggcaacgga agccgagacg gtgtcgtcca tgacagtctg ccagtggtaa
     1201 acgtggggat cagcaatagc acagatgaaa tcacgccagg tagtgtactg accaatgccc
     1261 tgggggccga aggggccgaa gcccgaggtc tgcgagagat cggcggcagc aatggcatcc
     1321 atggcctcag cgacgggttg cagaacagcg ggcagttcgg tttcgggcag gtcctggagg
     1381 gtgacaccct gggcacggcg ggagatgcag taggtcagcg actccgaaaa ttcgccaatg
     1441 tcgaggactt cgggaattgg gagggcggcc gaggcaaagt ggcggtagac gtaacgatcc
     1501 ttgtagaaac catcggcgca cgagttgacg cgcaggacgt agccacggcc gccgacatcg
     1561 aacgagaaag cacgcgattc ttcgccctcc gagagctgca tcaggtcgga gaccgagtcg
     1621 aacttttcga tcagaaactt ctcgaccgag gtggcggtga gttcgggctt cttcattttg
     1681 gtgggttggt gggatttaac taggttaaaa gactgatctg aaactcgtcc aaaacgggcc
     1741 gccttaaata cccccagcgg gtcgcagcac gatttcaggt cagtctgcca tggcagtttg
     1801 acctgaaaaa cctgtttgtt gactgcggtt tccaaagatg gcaagccgca gtggaaaaaa
     1861 acaaacaatc agcatttttg tgggatcatt gggacgtcag gtggcacttt tcggggaaat
     1921 gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg
     1981 agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa
     2041 catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac
     2101 ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac
     2161 atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt
     2221 ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc
     2281 gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca
     2341 ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc
     2401 ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag
     2461 gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa
     2521 ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg
     2581 gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa
     2641 ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg
     2701 gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt
     2761 gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt
     2821 caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag
     2881 cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat
     2941 ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct
     3001 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     3061 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     3121 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     3181 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
     3241 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     3301 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     3361 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     3421 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     3481 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     3541 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     3601 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     3661 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttccgatg
     3721 ccggcggtga gaacagcact agcaatgagc aatcacacgc atctaggtgc caggcgcagc
     3781 atgattgaaa atggcaacac tttcgccgca caaagtcaaa ccatttcatt ccacttgttc
     3841 tccatttccc cattccttgc tcatccctcc actgtaaaaa caactcgtcg tgtttcttgc
     3901 cataatggaa tatattgcgc tcataaccaa cgcaaaaaaa atgacaacca aaaaaaactt
     3961 caatgcaagc aacaaaaatc atgaaatccc tttttggtct tagaaatgat tgaaataaac
     4021 aaatgtgtct tggcaaacac aaaatgcggc cgccttaaga cttcttgtac agctcgtcca
     4081 tgccgccggt ggagtggcgg ccctcggagc gttcgtactg ttcgacgacg gtgtagtcct
     4141 cgttgtggga ggtgatgtcg agcttacggt cgacgttgta ggcaccgggc atctggacgg
     4201 gcttcttggc cttgtaggtg gtcttgaagt cggccaggta gcggccgccg tccttcaggc
     4261 gcagagccat cttaatgtcg cccttcagga caccgtcctc ggggtagaga cgctcggtgg
     4321 aggcttccca gcccatggtc ttcttctgca tgacggggcc gtcggggggg aagttggtgc
     4381 cgcggagctt gaccttgtag atcagggtgc cgtcctccag ggaggtgtcc tgggtgacgg
     4441 tgacggcacc gccgtcctcg aagttcatga cgcgctccca cttgaagccc tcggggaagg
     4501 actgcttgta gtagtcgggg atgtcggcgg ggtgcttggt gaaagcacgg gagccgtaca
     4561 tgaactgggg ggacaggatg tcccaggaga agggcagggg accacccttg gtgaccttca
     4621 gcttggcggt ctgggtgccc tcgtaggggc ggccctcgcc ctcaccctcg atctcgaact
     4681 cgtggccgtt catggagccc tccatgtgga ccttgaagcg catgaactcc ttgatgacgg
     4741 cctcgccctt cgagaccatg gtaccggtgg cgaccggggg atcgccctcc tccttggaga
     4801 tggactcgaa cttcttgatg aggtcggcca cgcccatgta ccactagttg tgaaggttgt
     4861 tctgtgtgtg ttaaataata aaaaaatgga agagtgagca tttccaaatc aaaaagcgac
     4921 aattatttaa atcaggattg cacgtgggga gaaggaagaa aacgatgaaa cagcatttta
     4981 aaaaaaaaca ctcgtggggt tcggcgtcaa caccccacgc tgacgttcgc gtttcgacgc
     5041 tcgtttttgg caatgcgcaa ggggatgggg atgaatggat cctaatccga gtgtgcacga
     5101 cgagagcgaa gaacaaacgc agaacgacag ttggaggaag tgttgaagag ggaaaaaaac
     5161 gaaaaaagaa aaaagtgaaa aaaaggcaat tttttgttgt tttccagaac
//
