LOCUS       sequence-346564-        2808 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  yeast MoClo level-0 part plasmid type 3 containing TPK2 gene.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2808)
  AUTHORS   Otto M, Skrekas C, Gossing M, Gustafsson J, Siewers V, David F
  TITLE     Expansion of the Yeast Modular Cloning Toolkit for CRISPR-Based 
            Applications, Genomic Integrations and Combinatorial Libraries.
  JOURNAL   ACS Synth Biol. 2021 Dec 17;10(12):3461-3474. doi: 
            10.1021/acssynbio.1c00408. Epub 2021 Dec 3.
  PUBMED    34860007
REFERENCE   2  (bases 1 to 2808)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.1c00408"; journalName: "ACS Synth Biol"; date: 
            "2021-12-17- 17"; volume: "10"; issue: "12"; pages: "3461-3474"
COMMENT     Sequence Label: pMC3-TPK2
FEATURES             Location/Qualifiers
     source          1..2808
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      157..745
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     646..665
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(2019..2053)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(2124..2783)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     2698..2717
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 ctcgataact caaaaaatac gcccggtagt gatcttattt cattatggtg aaagttggaa
       61 cctcttacgt gcccgatcaa tcatgaccaa aatcccttaa cgtgagtttt cgttccactg
      121 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt
      181 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca
      241 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac
      301 tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac
      361 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct
      421 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg
      481 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca
      541 gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt
      601 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta
      661 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc
      721 gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc
      781 cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa
      841 ccgtagtcgg tctcatatgg aattcgttgc agaaagggct cagccagttg gtcaaacaat
      901 ccagcagcaa aatgttaata cttacgggca aggcgtccta caaccgcatc atgatttaca
      961 gcagcgacaa caacaacaac agcagcgtca gcatcaacaa ctgctgacgt cacagttgcc
     1021 ccagaaatct ctcgtatcca aaggcaaata tacactacat gacttccaga ttatgagaac
     1081 gcttggtact ggatcttttg gtagggttca tttggtgcgc tctgttcaca atggtcggta
     1141 ttatgctata aaagttttaa agaaacaaca agttgtcaag atgaaacagg ttgaacatac
     1201 caatgacgaa cgacgtatgc taaagcttgt ggagcatccg tttctgatta gaatgtgggg
     1261 tacgtttcaa gatgctagga atatctttat ggtgatggat tatatcgaag gtggtgaact
     1321 tttctcgtta ctgagaaagt cacaaagatt tcctaatcct gtagcaaaat tttacgctgc
     1381 ggaagtcata ctggcattag agtacttgca tgctcataat atcatctaca gggatttaaa
     1441 gccagaaaat atcttgctgg atagaaatgg ccacattaaa ataaccgatt ttgggttcgc
     1501 caaagaggta caaactgtca catggacgct ttgtgggact cctgattaca ttgctcctga
     1561 agttattacc acaaagccat ataataagtc ggtggattgg tggtccctag gtgttctaat
     1621 ctacgaaatg ttagctggtt atacaccctt ttacgatact actccaatga agacgtatga
     1681 aaagatttta caaggtaaag tagtatatcc accatatttt catcccgacg tcgtggatct
     1741 actaagcaaa ctgatcactg cagatttgac aagaagaatc ggtaatttac aaagcggttc
     1801 cagggacatc aaagctcacc catggtttag tgaagttgta tgggaaagat tgttagcaaa
     1861 ggatattgaa actccatacg agcctcctat cacatcaggt atcggtgaca cgtctttatt
     1921 cgatcaatat cccgaggagc agctagatta tggtattcaa ggcgatgatc catatgctga
     1981 atactttcaa gatttctaag gatcctgaga ccagaccaat aaaaaacgcc cggcggcaac
     2041 cgagcgttct gaacaaatcc agatggagtt ctgaggtcat tactggatct atcaacagga
     2101 gtccaagcga gctcgatatc aaattacgcc ccgccctgcc actcatcgca gtactgttgt
     2161 aattcattaa gcattctgcc gacatggaag ccatcacaaa cggcatgatg aacctgaatc
     2221 gccagcggca tcagcacctt gtcgccttgc gtataatatt tgcccatggt gaaaacgggg
     2281 gcgaagaagt tgtccatatt ggccacgttt aaatcaaaac tggtgaaact cacccaggga
     2341 ttggctgaaa cgaaaaacat attctcaata aaccctttag ggaaataggc caggttttca
     2401 ccgtaacacg ccacatcttg cgaatatatg tgtagaaact gccggaaatc gtcgtggtat
     2461 tcactccaga gcgatgaaaa cgtttcagtt tgctcatgga aaacggtgta acaagggtga
     2521 acactatccc atatcaccag ctcaccgtct ttcattgcca tacgaaattc cggatgagca
     2581 ttcatcaggc gggcaagaat gtgaataaag gccggataaa acttgtgctt atttttcttt
     2641 acggtcttta aaaaggccgt aatatccagc tgaacggtct ggttataggt acattgagca
     2701 actgactgaa atgcctcaaa atgttcttta cgatgccatt gggatatatc aacggtggta
     2761 tatccagtga tttttttctc cattttagct tccttagctc ctgaaaat
//
