LOCUS       sequence-346565-        4063 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  For generating RNA probes for in situ hybridisation. Parton lab 
            clone ISB.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4063)
  AUTHORS   Lo HP, Lim YW, Xiong Z, Martel N, Ferguson C, Ariotti N, Giacomotto 
            J, Rae J, Floetenmeyer M, Moradi SV, Gao Y, Tillu VA, Xia D, Wang H,
            Rahnama S, Nixon SJ, Bastiani M, Day RD, Smith KA, Palpant NJ, 
            Johnston WA, Alexandrov K, Collins BM, Hall TE, Parton RG
  TITLE     Cavin4 interacts with Bin1 to promote T-tubule formation and 
            stability in developing skeletal muscle.
  JOURNAL   J Cell Biol. 2021 Dec 6;220(12). pii: 212693. doi: 
            10.1083/jcb.201905065. Epub 2021 Oct 11.
  PUBMED    34633413
REFERENCE   2  (bases 1 to 4063)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol."; date: "2021-12-6"; volume: "220(12). pii: 212693. doi"; 
            pages: " 10.1083/jcb.201905065. Epub 2021 Oct 11"
COMMENT     Sequence Label: Cavin4a in pBluescript(SK+)
FEATURES             Location/Qualifiers
     source          1..4063
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    105..126
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(141..146,147..164,165..171)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    179..195
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     184..206
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     203..219
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     203..219
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     238..258
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        240..258
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     295..311
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     295..311
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(345..361)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(346..362)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     complement(1488..1507)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1489..1507)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1517..1534)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(1517..1533)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1526..1548)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      1675..2130
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1762..1781)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1972..1993
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        2156..2260
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2261..2329,2330..3121)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2479..2498)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3292..3880
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3781..3800
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4034..4051
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac
       61 gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt gagttagctc
      121 actcattagg caccccaggc tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt
      181 gtgagcggat aacaatttca cacaggaaac agctatgacc atgattacgc caagcgcgca
      241 attaaccctc actaaaggga acaaaagctg gagctccacc gcggtggcgg ccgctctaga
      301 actagtggat cccccgggct gcaggaattc gatatcaagc ttatcgatac cgtcgacctc
      361 gagcacatgt ggcctttttt tttttttttt tttttttttt tttcatattg agattttttt
      421 aatacatttc acatgtggct ttatggtaac agaaattaca caaacacatc tagtgcttgc
      481 actgtttatc aacttggtgc ccaacacaca tactcataca ctctcacatt ttatgaaaaa
      541 aaatcttgtg ttgcttccac ttactcatga ccaattttct ctgcatcttc catcatatct
      601 ccacagtttt tgtggtgaac ggctcttttc agtcctcatg gatacgagtt gctccctcct
      661 ctgatactgg accctccctt ttgttttcgg taaccacctc tgtgtaggtc acatctgggc
      721 tggacttcct gcctttgggt ggagtcacag caggttccgc ctccgcaccc tcctggcctt
      781 cagcgaccgt gcgctccttc ttcagcttga ttttgaagga ttccttggtg ggcgctttct
      841 tagcgatgtt ttccttcagc ctctcgctgc tctgctttat cttctctcgc tgctcgggtg
      901 gcacgatctt ctcacccaaa gtgttgaatt tggttccaag cgtctggccg gtcttactca
      961 ggctctcctt ggtcttgctg aggttttctc gggtcttctc gcgggtcttg ttcatgttct
     1021 ctttggagaa cgcagccttg atgttctcga tgccttttaa cccggactgt ttgaggcggg
     1081 cgcccctgga cgactccgca tcctccacca ccatgtactc ttcatcagat gacaggtcag
     1141 cagggatgtc atactcatct ggctccacct ccaactcagc aagccctgct cccttgggag
     1201 tcttggtgac agcaacagag gggacttctt tttcaccctg atagatcaca accctgaact
     1261 tgttgcgtgt gagcagctca tcttgcgtag tttccacttt cttcacgcgc acgttctgtt
     1321 tctccacacg tgaacgtact tctttaacgt gtgtgctcac acgccgggcc ttctgcagca
     1381 gtttgtcgac ggtcgtcgct gtggccccgt ggtcacgagc cagtttgaga agctcggact
     1441 ggacagcgct gaccgagccc tcgagggggg gcccggtacc caattcgccc tatagtgagt
     1501 cgtattacgc gcgctcactg gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg
     1561 ttacccaact taatcgcctt gcagcacatc cccctttcgc cagctggcgt aatagcgaag
     1621 aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa tgggacgcgc
     1681 cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac
     1741 ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg
     1801 ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt
     1861 tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc
     1921 cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct
     1981 tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga
     2041 ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga
     2101 attttaacaa aatattaacg cttacaattt aggtggcact tttcggggaa atgtgcgcgg
     2161 aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata
     2221 accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg
     2281 tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac
     2341 gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact
     2401 ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat
     2461 gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga
     2521 gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac
     2581 agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat
     2641 gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac
     2701 cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct
     2761 gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac
     2821 gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga
     2881 ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg
     2941 gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact
     3001 ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac
     3061 tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta
     3121 actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt
     3181 taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga
     3241 gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc
     3301 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt
     3361 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc
     3421 gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc
     3481 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg
     3541 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg
     3601 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga
     3661 actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc
     3721 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg
     3781 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg
     3841 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt
     3901 tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc
     3961 tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg
     4021 aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gag
//
