LOCUS       sequence-346566-       11874 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pNOC episomal plasmid harboring the humanized lbCas12a gene sequence
            tagged with Nlux under the control of Ribi promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11874)
  AUTHORS   Naduthodi MIS, Sudfeld C, Avitzigiannis EK, Trevisan N, van Lith E, 
            Alcaide Sancho J, D'Adamo S, Barbosa M, van der Oost J
  TITLE     Comprehensive Genome Engineering Toolbox for Microalgae 
            Nannochloropsis oceanica Based on CRISPR-Cas Systems.
  JOURNAL   ACS Synth Biol. 2021 Dec 17;10(12):3369-3378. doi: 
            10.1021/acssynbio.1c00329. Epub 2021 Nov 18.
  PUBMED    34793143
REFERENCE   2  (bases 1 to 11874)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.1c00329"; journalName: "ACS Synth Biol"; date: 
            "2021-12-17- 17"; volume: "10"; issue: "12"; pages: "3369-3378"
COMMENT     Sequence Label: pNOC_hlbCas12a-Nlux
FEATURES             Location/Qualifiers
     source          1..11874
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     76..95
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(314..418)
                     /gene="bla"
                     /label=AmpR promoter
     misc_feature    455..958
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
     ncRNA           complement(1987..2054)
                     /product="hepatitis delta virus self-cleaving ribozyme 
                     (Ferre-D'Amare and Scott, 2010)"
                     /label=HDV ribozyme
                     /ncRNA_class="ribozyme"
     ncRNA           complement(2112..2148)
                     /product="self-cleaving hammerhead ribozyme (Ferre-D'Amare 
                     and Scott, 2010)"
                     /label=hammerhead ribozyme
                     /ncRNA_class="hammerhead_ribozyme"
     CDS             3123..3134
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             3355..3375
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3376..7056
                     /codon_start=1
                     /product="CRISPR-associated protein from Lachnospiraceae 
                     bacterium ND2006 (Zetsche et al., 2015)"
                     /label=LbCpf1
                     /translation="SKLEKFTNCYSLSKTLRFKAIPVGKTQENIDNKRLLVEDEKRAED
                     YKGVKKLLDRYYLSFINDVLHSIKLKNLNNYISLFRKKTRTEKENKELENLEINLRKEI
                     AKAFKGNEGYKSLFKKDIIETILPEFLDDKDEIALVNSFNGFTTAFTGFFDNRENMFSE
                     EAKSTSIAFRCINENLTRYISNMDIFEKVDAIFDKHEVQEIKEKILNSDYDVEDFFEGE
                     FFNFVLTQEGIDVYNAIIGGFVTESGEKIKGLNEYINLYNQKTKQKLPKFKPLYKQVLS
                     DRESLSFYGEGYTSDEEVLEVFRNTLNKNSEIFSSIKKLEKLFKNFDEYSSAGIFVKNG
                     PAISTISKDIFGEWNVIRDKWNAEYDDIHLKKKAVVTEKYEDDRRKSFKKIGSFSLEQL
                     QEYADADLSVVEKLKEIIIQKVDEIYKVYGSSEKLFDADFVLEKSLKKNDAVVAIMKDL
                     LDSVKSFENYIKAFFGEGKETNRDESFYGDFVLAYDILLKVDHIYDAIRNYVTQKPYSK
                     DKFKLYFQNPQFMGGWDKDKETDYRATILRYGSKYYLAIMDKKYAKCLQKIDKDDVNGN
                     YEKINYKLLPGPNKMLPKVFFSKKWMAYYNPSEDIQKIYKNGTFKKGDMFNLNDCHKLI
                     DFFKDSISRYPKWSNAYDFNFSETEKYKDIAGFYREVEEQGYKVSFESASKKEVDKLVE
                     EGKLYMFQIYNKDFSDKSHGTPNLHTMYFKLLFDENNHGQIRLSGGAELFMRRASLKKE
                     ELVVHPANSPIANKNPDNPKKTTTLSYDVYKDKRFSEDQYELHIPIAINKCPKNIFKIN
                     TEVRVLLKHDDNPYVIGIDRGERNLLYIVVVDGKGNIVEQYSLNEIINNFNGIRIKTDY
                     HSLLDKKEKERFEARQNWTSIENIKELKAGYISQVVHKICELVEKYDAVIALEDLNSGF
                     KNSRVKVEKQVYQKFEKMLIDKLNYMVDKKSNPCATGGALKGYQITNKFESFKSMSTQN
                     GFIFYIPAWLTSKIDPSTGFVNLLKTKYTSIADSKKFISSFDRIMYVPEEDLFEFALDY
                     KNFSRTDADYIKKWKLYSYGNRIRIFRNPKKNNVFDWEEVCLTSAYKELFNKYGINYQQ
                     GDIRALLCEQSDKAFYSSFMALMSLMLQMRNSITGRTDVDFLISPVKNSDGIFYDSRNY
                     EAQENAILPKNADANGAYNIARKVLWAIGQFKKAEDEKLDKVKIAISNKEWLEYAQTSV
                     KH"
     CDS             7597..7623
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     regulatory      7625..7634
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             7639..7659
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     regulatory      8340..8349
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(9234..9608)
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     rep_origin      complement(10568..11156)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10648..10667)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(11327..11874,1..244,245..313))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc
       61 cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg
      121 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga
      181 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg
      241 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat
      301 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc
      361 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca
      421 catttccccg aaaagtgcca cctgggtcct tttcatcacg tgctataaaa ataattataa
      481 tttaaatttt ttaatataaa tatataaatt aaaaatagaa agtaaaaaaa gaaattaaag
      541 aaaaaatagt ttttgttttc cgaagatgta aaagactcta gggggatcgc caacaaatac
      601 taccttttat cttgtacttc ctgctctcag gtattaatgc cgaattgttt catcttgtct
      661 gtgtagaaga ccacacacga aaatcctgtg attttacatt ttacttatcg ttaatcgaat
      721 gtatatctat ttaatctgct tttcttgtct aataaatata tatgtaaagt acgctttttg
      781 ttgaaatttt ttaaaccttt gtttattttt ttttcttcat tccgtaactc ttctaccttc
      841 tttatttact ttctaaaatc caaatacaaa acataaaaat aaataaacac agagtaaatt
      901 cccaaattat tccatcatta aaagatacga ggcgcgtgta agttacaggc aagcgatccg
      961 tctaagaaac cggtgcaaag ctgacgccct tttcgtcgtc gttgctggtg ttaaaattgc
     1021 gcgtttcttt tttttccgtc tttgtcgtct tttgactttt ttatcttctt ttctgtcttc
     1081 ttttattctt ctcttcttct tcatcactcc ctcttcattt ttcttcttct ttttcctcgt
     1141 ctattgtcct cgggcattac cagttgtgct gctcacgtgc gcgcaagcgg tgaaagggtg
     1201 agaggataca cggacaacaa tcattgaaaa ttacgcttaa agccggtgcc tggaaaagtc
     1261 cacgcgcaga tggcctttgc ttgccttgct gctgctgcag ctgctgctgc tgtcgcctgt
     1321 gatggtaatg gttgttgttg tggtcctgct tgcttgctag tttgcattgc gtttgcggtt
     1381 gccttatgac cgactccgtg tgattgatcc ttaaacatat tttatcattg atgtggattg
     1441 tgtgacgacg gcaaaaaaga attactttac acatgactat cacacctgct cccctccctt
     1501 ctcctttcaa tcggtcaact tcccaaccgc tcctcgaaaa tatttcagtt gaatcctcta
     1561 ctacgtaccc cttacttaaa tttactttct ctctccccat caatgcgccg ttgttctgaa
     1621 gttgtattat tgatgggaga tgattggcac tgcaccagac ctggccaaaa gccaccacag
     1681 gtggccacat actcttgcat gggtatactc taccgaggca aagattaaag tgcacgtccc
     1741 ctgcccctct ctctccctac ttccttcagt ctcagtggtt ccgtgcccgt tcagcactac
     1801 atcgccctgc ccgctgttcc ttctcaaaca gccacccatc ccaacccaca tacacagaaa
     1861 caacctttcc tacacatttt cccaagtctt ctcttcacta ttctacacta atacaccacc
     1921 gtgatatgcc ggttatctag atgtcctttc tcctttctca tcctttcttt cccatggtac
     1981 ccaggagtcc cattcgccat gccgaagcat gttgcccagc cggcgccagc gaggaggctg
     2041 ggaccatgcc ggccggtacc cttgaggagg gggatactcg tgccaatcta caacagtaga
     2101 aattgctctt cgacgagctt actcgtttcg tcctcacgga ctcatcagaa tttcatcgat
     2161 tctgtggatg gagggaggga aggagggggg gagtgagtga gtcgagagaa cgacgactgg
     2221 ggcaaagaga ggatgacgcc actgcaacaa aagacaggga agaatagagg ctgttggaaa
     2281 acaggaacac agtgaagaat agaggctgca gggtaacagg cacatttatc gcagagaggc
     2341 gggcggcagg ggcgatacgt aaagagaccc ctaaacttct ccgcgtcatc cgttcacgct
     2401 acctccttcc ctccctttac tcccattgat caccaaggca gcgcccgcag ccaccagcag
     2461 ccaccgtcct cagtgtcaca gctagacgcg tccccatcag gcagcagacc accagaatat
     2521 gcgctttcgc ttcccatcat atccctccct cctccccttc tattcctcat catgcatcat
     2581 ttctttctcc ttgctgcatg cccctcttct taccttggct aagatctacc ggaggaagaa
     2641 gtcttcaacg atagataaat gcctgtatcg tgatggttgt ttgaatggtg gaagtgagcc
     2701 aggtattgag aacgaggggc gcaagggtgc gtggaagaag gcagggagag gcgaggcgaa
     2761 ggagaagggc atgtggtggg aacgaagcct ccacaccaca gccacccatg cttttctctg
     2821 ttcgagttcc cgggttctac tctcacacac tacacgtgca cagaaaacat tcacgcacaa
     2881 cagagataca gagggatccg tcgagatggt ctacacgtcc taatgtcccc tgctttcgaa
     2941 gcccagagcc atcgctttct gtttttatcc attcgcaaca catcctgttc tcgcgacctc
     3001 tcctcttcct ggccaccatc gcttctccgt gccatgccct gtacatcctt ccctccctcg
     3061 ttgtctacca cactaatcat gcgctctgca gcagacactc ggctactatt tgtgtcgtac
     3121 ggattgaggg gagggagggg aagggaggca agaagtaaat cggtggtcaa caacagacgc
     3181 ggtctcccca ccctccctca ttcctttccc ctcctataat gagcgaagaa agccatggac
     3241 accgagtcac ccactctcct aatccaccac caccagggca ctaacggact cacgctcacc
     3301 aaactcggta acaatatcca ctgcacacag atacacacgc aaccctcgag catgcccaag
     3361 aaaaagcgga aggtgagcaa gctggagaag tttacaaact gctactccct gtctaagacc
     3421 ctgaggttca aggccatccc tgtgggcaag acccaggaga acatcgacaa taagcggctg
     3481 ctggtggagg acgagaagag agccgaggat tataagggcg tgaagaagct gctggatcgc
     3541 tactatctgt cttttatcaa cgacgtgctg cacagcatca agctgaagaa tctgaacaat
     3601 tacatcagcc tgttccggaa gaaaaccaga accgagaagg agaataagga gctggagaac
     3661 ctggagatca atctgcggaa ggagatcgcc aaggccttca agggcaacga gggctacaag
     3721 tccctgttta agaaggatat catcgagaca atcctgccag agttcctgga cgataaggac
     3781 gagatcgccc tggtgaacag cttcaatggc tttaccacag ccttcaccgg cttctttgat
     3841 aacagagaga atatgttttc cgaggaggcc aagagcacat ccatcgcctt caggtgtatc
     3901 aacgagaatc tgacccgcta catctctaat atggacatct tcgagaaggt ggacgccatc
     3961 tttgataagc acgaggtgca ggagatcaag gagaagatcc tgaacagcga ctatgatgtg
     4021 gaggatttct ttgagggcga gttctttaac tttgtgctga cacaggaggg catcgacgtg
     4081 tataacgcca tcatcggcgg cttcgtgacc gagagcggcg agaagatcaa gggcctgaac
     4141 gagtacatca acctgtataa tcagaaaacc aagcagaagc tgcctaagtt taagccactg
     4201 tataagcagg tgctgagcga tcgggagtct ctgagcttct acggcgaggg ctatacatcc
     4261 gatgaggagg tgctggaggt gtttagaaac accctgaaca agaacagcga gatcttcagc
     4321 tccatcaaga agctggagaa gctgttcaag aattttgacg agtactctag cgccggcatc
     4381 tttgtgaaga acggccccgc catcagcaca atctccaagg atatcttcgg cgagtggaac
     4441 gtgatccggg acaagtggaa tgccgagtat gacgatatcc acctgaagaa gaaggccgtg
     4501 gtgaccgaga agtacgagga cgatcggaga aagtccttca agaagatcgg ctccttttct
     4561 ctggagcagc tgcaggagta cgccgacgcc gatctgtctg tggtggagaa gctgaaggag
     4621 atcatcatcc agaaggtgga tgagatctac aaggtgtatg gctcctctga gaagctgttc
     4681 gacgccgatt ttgtgctgga gaagagcctg aagaagaacg acgccgtggt ggccatcatg
     4741 aaggacctgc tggattctgt gaagagcttc gagaattaca tcaaggcctt ctttggcgag
     4801 ggcaaggaga caaacaggga cgagtccttc tatggcgatt ttgtgctggc ctacgacatc
     4861 ctgctgaagg tggaccacat ctacgatgcc atccgcaatt atgtgaccca gaagccctac
     4921 tctaaggata agttcaagct gtattttcag aaccctcagt tcatgggcgg ctgggacaag
     4981 gataaggaga cagactatcg ggccaccatc ctgagatacg gctccaagta ctatctggcc
     5041 atcatggata agaagtacgc caagtgcctg cagaagatcg acaaggacga tgtgaacggc
     5101 aattacgaga agatcaacta taagctgctg cccggcccta ataagatgct gccaaaggtg
     5161 ttcttttcta agaagtggat ggcctactat aaccccagcg aggacatcca gaagatctac
     5221 aagaatggca cattcaagaa gggcgatatg tttaacctga atgactgtca caagctgatc
     5281 gacttcttta aggatagcat ctcccggtat ccaaagtggt ccaatgccta cgatttcaac
     5341 ttttctgaga cagagaagta taaggacatc gccggctttt acagagaggt ggaggagcag
     5401 ggctataagg tgagcttcga gtctgccagc aagaaggagg tggataagct ggtggaggag
     5461 ggcaagctgt atatgttcca gatctataac aaggactttt ccgataagtc tcacggcaca
     5521 cccaatctgc acaccatgta cttcaagctg ctgtttgacg agaacaatca cggacagatc
     5581 aggctgagcg gaggagcaga gctgttcatg aggcgcgcct ccctgaagaa ggaggagctg
     5641 gtggtgcacc cagccaactc ccctatcgcc aacaagaatc cagataatcc caagaaaacc
     5701 acaaccctgt cctacgacgt gtataaggat aagaggtttt ctgaggacca gtacgagctg
     5761 cacatcccaa tcgccatcaa taagtgcccc aagaacatct tcaagatcaa tacagaggtg
     5821 cgcgtgctgc tgaagcacga cgataacccc tatgtgatcg gcatcgatag gggcgagcgc
     5881 aatctgctgt atatcgtggt ggtggacggc aagggcaaca tcgtggagca gtattccctg
     5941 aacgagatca tcaacaactt caacggcatc aggatcaaga cagattacca ctctctgctg
     6001 gacaagaagg agaaggagag gttcgaggcc cgccagaact ggacctccat cgagaatatc
     6061 aaggagctga aggccggcta tatctctcag gtggtgcaca agatctgcga gctggtggag
     6121 aagtacgatg ccgtgatcgc cctggaggac ctgaactctg gctttaagaa tagccgcgtg
     6181 aaggtggaga agcaggtgta tcagaagttc gagaagatgc tgatcgataa gctgaactac
     6241 atggtggaca agaagtctaa tccttgtgca acaggcggcg ccctgaaggg ctatcagatc
     6301 accaataagt tcgagagctt taagtccatg tctacccaga acggcttcat cttttacatc
     6361 cctgcctggc tgacatccaa gatcgatcca tctaccggct ttgtgaacct gctgaaaacc
     6421 aagtatacca gcatcgccga ttccaagaag ttcatcagct cctttgacag gatcatgtac
     6481 gtgcccgagg aggatctgtt cgagtttgcc ctggactata agaacttctc tcgcacagac
     6541 gccgattaca tcaagaagtg gaagctgtac tcctacggca accggatcag aatcttccgg
     6601 aatcctaaga agaacaacgt gttcgactgg gaggaggtgt gcctgaccag cgcctataag
     6661 gagctgttca acaagtacgg catcaattat cagcagggcg atatcagagc cctgctgtgc
     6721 gagcagtccg acaaggcctt ctactctagc tttatggccc tgatgagcct gatgctgcag
     6781 atgcggaaca gcatcacagg ccgcaccgac gtggattttc tgatcagccc tgtgaagaac
     6841 tccgacggca tcttctacga tagccggaac tatgaggccc aggagaatgc catcctgcca
     6901 aagaacgccg acgccaatgg cgcctataac atcgccagaa aggtgctgtg ggccatcggc
     6961 cagttcaaga aggccgagga cgagaagctg gataaggtga agatcgccat ctctaacaag
     7021 gagtggctgg agtacgccca gaccagcgtg aagcacgggt ccggcagcgg gtccgttaac
     7081 atggtgttta ctctcgagga cttcgtgggc gactggcggc agacagctgg ctataacctc
     7141 gaccaggtgc tggagcaggg cggtgtgtcc tcgcttttcc agaacctggg cgtctccgtg
     7201 accccaatcc agcgcatcgt cctgagcggc gagaacggcc tgaagatcga catccacgtc
     7261 atcattccct acgagggcct gagcggggac caaatgggcc agatcgagaa gatcttcaag
     7321 gttgtgtacc ccgtggacga tcaccacttc aaggtgatac tgcactacgg gacgctggtc
     7381 atcgacggtg tgacgccgaa catgattgac tacttcggcc gtccttacga gggcattgcc
     7441 gtgttcgacg gcaagaagat cacggtgacc ggcaccctgt ggaacggcaa taagatcatt
     7501 gacgagcggc tgatcaaccc ggatggctcg ctgttgttcc gcgtgaccat caacggggta
     7561 accggctggc gcctgtgcga gcgcatcctg gccggatacc cctacgacgt gcccgactac
     7621 gcgtccatgg tggctagccc gaaaaagaag aggaaggtct gacaattgga aagatccaag
     7681 agagacgagt agagattttt tttttgggat tgatgtttgt cgttctttga gttgtcgtcg
     7741 agttacgcct tttgtaagaa tgttccgcag gagaggagga ggatgggcat gagtgagggt
     7801 gagagggctt gcccgctttt ttttttaaaa acgctgaaga cgtggttgtc aaacaaaccc
     7861 cccatagaaa cgattttgtt acggtgcggt ccagacgtca cttgaatggc tccgcggaaa
     7921 ggccagggag ggaagggggg agggaggaaa catgaaacat gttgaacggc tcaacagggt
     7981 ttgggggaca agagaggtag cgccctgatg gactgctccc tcccctcctt tccctcaatg
     8041 tctcattcat ccatgcttcc cccttctctc tctcccctcc gttccatccc ccgcgggcgt
     8101 ggtagtggcg tgatgggatc cactaaaatg tacgtgtaag aaaagccggt gagcttacgc
     8161 ttttgtgaaa gtgggagtac gagtgttgtg tgtgtgtgta gtggtttcag accccagaca
     8221 gaggcgaagc agaaaaagca gacgatgaag acgacgaaga aatgagcagt ctatttttat
     8281 cgtggaaaca gaagaggtga tatcgtctcc tgcaggcatg caagctgatc cactagaggc
     8341 catggcggcc gcgagggtgg acttgagagt gtggaagtga gtgagggtag aagatagagt
     8401 aaggatggca gacaagatga cgataaagaa gacgtggaag agagggaaat acacgtacaa
     8461 aggaagaaaa caagaaaagg aaaaagagga aaaaataaac gcaggcggaa ttactcactg
     8521 ttgaaatggt ctctcgtcct gacacggaag ttgtacgaac aacacgcatg cacacaacgt
     8581 actcttcttc tgtgttctaa ataaagacta tttgctaagg gagggtagtg gcgatggtgg
     8641 cgtacagtga cttaatgggg aggagcaaat caatgatgcc ttttttggca tttttctatt
     8701 gtctatctgc ggtactacaa ttcgttgtta tacagtcacg gatggaagga agaagcagca
     8761 gtagagtagg ggataaacca atgagataag ggagctgatg taacgagacc aggacgcctg
     8821 aaattaaaga tagaaaaacc acaagtagag ccaggagcga tgaccatctt ccatttcgtt
     8881 tcttcacaac agtgcttttg ctttcttcgc ttctactctc tccttactgg ctaccatgac
     8941 attgttcact tctttcattt ctccttccct ctctcacaca catcccaccc cttcgatttc
     9001 cttctgccct tcttccctcg gggtctagtc ttattttgat tgaaccgatg cgtgaattgg
     9061 atcgagtgac ttgtatgacg ggcacacaca cacatgacga cgacaggtga ggacttgaag
     9121 gacctaaggc atgacaccta aaaggaagga aggccgaaag acgacggcag aagtggggat
     9181 ctctgaaacc agatcccttc tcctcttcac tccctcctcc tgatccctgg ccatcagtcc
     9241 tgctcctcgg ccacgaagtg cacgcagttg ccggccgggt cgcgcagggc gaactcccgc
     9301 ccccacggct gctcgccgat ctcggtcatg gccggcccgg aggcgtcccg gaagttcgtg
     9361 gacacgacct ccgaccactc ggcgtacagc tcgtccaggc cgcgcaccca cacccaggcc
     9421 agggtgttgt ccggcaccac ctggtcctgg accgcgctga tgaacagggt cacgtcgtcc
     9481 cggaccacac cggcgaagtc gtcctccacg aagtcccggg agaacccgag ccggtcggtc
     9541 cagaactcga ccgctccggc gacgtcgcgc gcggtgagca ccggaacggc actggtcaac
     9601 ttggccatct gctcggaggg gaggatgaag ggaaggaggg aggggggagg ggcatttatg
     9661 acaattagaa cttcagaggt ctactccttg tgtgtaacga ggtgaacgac gggtgaaagt
     9721 gttcgcctga caggactggt cgaagatcgg ggggaggggg ctacctctat ctctgagggc
     9781 atctttctga taaggtgcct tcaaccgcaa ccccgaaaac ccctgccccc caccacacca
     9841 tggacacatg cacgcacgac aaatagacag agcgatgcca acaggattgg ttgtatagta
     9901 tggttctggt aagatagagg gattaaatgc cctgctccag gccgtgaggg gcgaactggc
     9961 gcgcttgtgc ttctccttct gtacttaatc tacatccctg accttgaact ggatcttgac
    10021 aaatatagat ccgaaaaccc actgccgcac gaacttacca taatttaaaa agaggtttta
    10081 gaggcgaaga gtggagactt ctgtgtgagg tttgtgaaaa ggtaagaagg aggggacagg
    10141 agatcatgaa tctatatctt aattaagcac ttctgctgtt ttgaatgggg ttagagcaca
    10201 agtgggatgc ctggactttc agggcatgca agcacacact acgaccacaa cagaagtcaa
    10261 aagggtgtag atagagtttg gcctcttgtc tgatgggcat tcacccggca gtatcctagt
    10321 taaaacaaag acacatgaag agatataaag atcatcgcgt gtgccctcta gacgggcggg
    10381 ctatagagga aaggacaaaa gaccgccaaa ccgacgcagg atgcggacag gttcagtgca
    10441 ccaaggagga ggaaacgccc aaccacgagg tccaaggctg gcagagaaat aaacgacccc
    10501 gtttaaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc
    10561 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc
    10621 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc
    10681 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt
    10741 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg
    10801 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat
    10861 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag
    10921 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt
    10981 ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc
    11041 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta
    11101 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag
    11161 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga
    11221 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa
    11281 gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa
    11341 tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc
    11401 ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga
    11461 taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa
    11521 gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt
    11581 gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg
    11641 ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc
    11701 aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg
    11761 gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag
    11821 cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actg
//
