LOCUS       sequence-346567-       12116 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pNOC episomal plasmid harboring the humanized fnCas12a gene sequence
            tagged with Nlux and crRNA targeting the Nitrate reductase gene of 
            N. oceanica IMET1 with spacer 2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12116)
  AUTHORS   Naduthodi MIS, Sudfeld C, Avitzigiannis EK, Trevisan N, van Lith E, 
            Alcaide Sancho J, D'Adamo S, Barbosa M, van der Oost J
  TITLE     Comprehensive Genome Engineering Toolbox for Microalgae 
            Nannochloropsis oceanica Based on CRISPR-Cas Systems.
  JOURNAL   ACS Synth Biol. 2021 Dec 17;10(12):3369-3378. doi: 
            10.1021/acssynbio.1c00329. Epub 2021 Nov 18.
  PUBMED    34793143
REFERENCE   2  (bases 1 to 12116)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.1c00329"; journalName: "ACS Synth Biol"; date: 
            "2021-12-17- 17"; volume: "10"; issue: "12"; pages: "3369-3378"
COMMENT     Sequence Label: pNOC_hfnCas12a-Nlux_crRNA NR2
FEATURES             Location/Qualifiers
     source          1..12116
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    87..590
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
     ncRNA           complement(1619..1686)
                     /product="hepatitis delta virus self-cleaving ribozyme 
                     (Ferre-D'Amare and Scott, 2010)"
                     /label=HDV ribozyme
                     /ncRNA_class="ribozyme"
     ncRNA           complement(1748..1784)
                     /product="self-cleaving hammerhead ribozyme (Ferre-D'Amare 
                     and Scott, 2010)"
                     /label=hammerhead ribozyme
                     /ncRNA_class="hammerhead_ribozyme"
     CDS             2781..2792
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             3013..3033
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             7471..7497
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     regulatory      7499..7508
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             7513..7533
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     regulatory      8214..8223
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(9108..9482)
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     rep_origin      complement(10442..11030)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10522..10541)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(11201..11992,11993..12061))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11824..11843
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(join(12062..12116,1..50))
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc
       61 cgaaaagtgc cacctgggtc cttttcatca cgtgctataa aaataattat aatttaaatt
      121 ttttaatata aatatataaa ttaaaaatag aaagtaaaaa aagaaattaa agaaaaaata
      181 gtttttgttt tccgaagatg taaaagactc tagggggatc gccaacaaat actacctttt
      241 atcttgtact tcctgctctc aggtattaat gccgaattgt ttcatcttgt ctgtgtagaa
      301 gaccacacac gaaaatcctg tgattttaca ttttacttat cgttaatcga atgtatatct
      361 atttaatctg cttttcttgt ctaataaata tatatgtaaa gtacgctttt tgttgaaatt
      421 ttttaaacct ttgtttattt ttttttcttc attccgtaac tcttctacct tctttattta
      481 ctttctaaaa tccaaataca aaacataaaa ataaataaac acagagtaaa ttcccaaatt
      541 attccatcat taaaagatac gaggcgcgtg taagttacag gcaagcgatc cgtctaagaa
      601 accggtgcaa agctgacgcc cttttcgtcg tcgttgctgg tgttaaaatt gcgcgtttct
      661 tttttttccg tctttgtcgt cttttgactt ttttatcttc ttttctgtct tcttttattc
      721 ttctcttctt cttcatcact ccctcttcat ttttcttctt ctttttcctc gtctattgtc
      781 ctcgggcatt accagttgtg ctgctcacgt gcgcgcaagc ggtgaaaggg tgagaggata
      841 cacggacaac aatcattgaa aattacgctt aaagccggtg cctggaaaag tccacgcgca
      901 gatggccttt gcttgccttg ctgctgctgc agctgctgct gctgtcgcct gtgatggtaa
      961 tggttgttgt tgtggtcctg cttgcttgct agtttgcatt gcgtttgcgg ttgccttatg
     1021 accgactccg tgtgattgat ccttaaacat attttatcat tgatgtggat tgtgtgacga
     1081 cggcaaaaaa gaattacttt acacatgact atcacacctg ctcccctccc ttctcctttc
     1141 aatcggtcaa cttcccaacc gctcctcgaa aatatttcag ttgaatcctc tactacgtac
     1201 cccttactta aatttacttt ctctctcccc atcaatgcgc cgttgttctg aagttgtatt
     1261 attgatggga gatgattggc actgcaccag acctggccaa aagccaccac aggtggccac
     1321 atactcttgc atgggtatac tctaccgagg caaagattaa agtgcacgtc ccctgcccct
     1381 ctctctccct acttccttca gtctcagtgg ttccgtgccc gttcagcact acatcgccct
     1441 gcccgctgtt ccttctcaaa cagccaccca tcccaaccca catacacaga aacaaccttt
     1501 cctacacatt ttcccaagtc ttctcttcac tattctacac taatacacca ccgtgatatg
     1561 ccggttatct agatgtcctt tctcctttct catcctttct ttcccatggt acccaggagt
     1621 cccattcgcc atgccgaagc atgttgccca gccggcgcca gcgaggaggc tgggaccatg
     1681 ccggcccttg aggaggggga tactcgtgcc aatctacaac agtagaaatt atttaaagtt
     1741 cttagacgac gagcttactc gtttcgtcct cacggactca tcaggtctaa gaagagccaa
     1801 ggcctgctct tcatcgattc tgtggatgga gggagggaag gaggggggga gtgagtgagt
     1861 cgagagaacg acgactgggg caaagagagg atgacgccac tgcaacaaaa gacagggaag
     1921 aatagaggct gttggaaaac aggaacacag tgaagaatag aggctgcagg gtaacaggca
     1981 catttatcgc agagaggcgg gcggcagggg cgatacgtaa agagacccct aaacttctcc
     2041 gcgtcatccg ttcacgctac ctccttccct ccctttactc ccattgatca ccaaggcagc
     2101 gcccgcagcc accagcagcc accgtcctca gtgtcacagc tagacgcgtc cccatcaggc
     2161 agcagaccac cagaatatgc gctttcgctt cccatcatat ccctccctcc tccccttcta
     2221 ttcctcatca tgcatcattt ctttctcctt gctgcatgcc cctcttctta ccttggctaa
     2281 gatctaccgg aggaagaagt cttcaacgat agataaatgc ctgtatcgtg atggttgttt
     2341 gaatggtgga agtgagccag gtattgagaa cgaggggcgc aagggtgcgt ggaagaaggc
     2401 agggagaggc gaggcgaagg agaagggcat gtggtgggaa cgaagcctcc acaccacagc
     2461 cacccatgct tttctctgtt cgagttcccg ggttctactc tcacacacta cacgtgcaca
     2521 gaaaacattc acgcacaaca gagatacaga gggatccgtc gagatggtct acacgtccta
     2581 atgtcccctg ctttcgaagc ccagagccat cgctttctgt ttttatccat tcgcaacaca
     2641 tcctgttctc gcgacctctc ctcttcctgg ccaccatcgc ttctccgtgc catgccctgt
     2701 acatccttcc ctccctcgtt gtctaccaca ctaatcatgc gctctgcagc agacactcgg
     2761 ctactatttg tgtcgtacgg attgagggga gggaggggaa gggaggcaag aagtaaatcg
     2821 gtggtcaaca acagacgcgg tctccccacc ctccctcatt cctttcccct cctataatga
     2881 gcgaagaaag ccatggacac cgagtcaccc actctcctaa tccaccacca ccagggcact
     2941 aacggactca cgctcaccaa actcggtaac aatatccact gcacacagat acacacgcaa
     3001 ccctcgagca tgcccaagaa aaagcggaag gtgagcatct accaggagtt cgtcaacaag
     3061 tattcactga gtaagacact gcggttcgag ctgatcccac agggcaagac actggagaac
     3121 atcaaggccc gaggcctgat tctggacgat gagaagcggg caaaagacta taagaaagcc
     3181 aagcagatca ttgataaata ccaccagttc tttatcgagg aaattctgag ctccgtgtgc
     3241 atcagtgagg atctgctgca gaattactca gacgtgtact tcaagctgaa gaagagcgac
     3301 gatgacaacc tgcagaagga cttcaagtcc gccaaggaca ccatcaagaa acagattagc
     3361 gagtacatca aggactccga aaagtttaaa aatctgttca accagaatct gatcgatgct
     3421 aagaaaggcc aggagtccga cctgatcctg tggctgaaac agtctaagga caatgggatt
     3481 gaactgttca aggctaactc cgatatcact gatattgacg aggcactgga aatcatcaag
     3541 agcttcaagg gatggaccac atactttaaa ggcttccacg agaaccgcaa gaacgtgtac
     3601 tccagcaacg acattcctac ctccatcatc taccgaatcg tcgatgacaa tctgccaaag
     3661 ttcctggaga acaaggccaa atatgaatct ctgaaggaca aagctcccga ggcaattaat
     3721 tacgaacaga tcaagaaaga tctggctgag gaactgacat tcgatatcga ctataagact
     3781 agcgaggtga accagagggt cttttccctg gacgaggtgt ttgaaatcgc caatttcaac
     3841 aattacctga accagtccgg cattactaaa ttcaatacca tcattggcgg gaagtttgtg
     3901 aacggggaga ataccaagcg caagggaatt aacgaataca tcaatctgta tagccagcag
     3961 atcaacgaca aaactctgaa gaaatacaag atgtctgtgc tgttcaaaca gatcctgagt
     4021 gataccgagt ccaagtcttt tgtcattgat aaactggaag atgactcaga cgtggtcact
     4081 accatgcaga gcttttatga gcagatcgcc gctttcaaga cagtggagga aaaatctatt
     4141 aaggaaactc tgagtctgct gttcgatgac ctgaaagccc agaagctgga cctgagtaag
     4201 atctacttca aaaacgataa gagtctgaca gacctgtcac agcaggtgtt tgatgactat
     4261 tccgtgattg ggaccgccgt cctggagtac attacacagc agatcgctcc aaagaacctg
     4321 gataatccct ctaagaaaga gcaggaactg atcgctaaga aaaccgagaa ggcaaaatat
     4381 ctgagtctgg aaacaattaa gctggcactg gaggagttca acaagcacag ggatattgac
     4441 aaacagtgcc gctttgagga aatcctggcc aacttcgcag ccatccccat gatttttgat
     4501 gagatcgccc agaacaaaga caatctggct cagatcagta ttaagtacca gaaccagggc
     4561 aagaaagacc tgctgcaggc ttcagcagaa gatgacgtga aagccatcaa ggatctgctg
     4621 gaccagacca acaatctgct gcacaagctg aaaatcttcc atattagtca gtcagaggat
     4681 aaggctaata tcctggataa agacgaacac ttctacctgg tgttcgagga atgttacttc
     4741 gagctggcaa acattgtccc cctgtataac aagattagga actacatcac acagaagcct
     4801 tactctgacg agaagtttaa actgaacttc gaaaatagta ccctggccaa cgggtgggat
     4861 aagaacaagg agcctgacaa cacagctatc ctgttcatca aggatgacaa gtactatctg
     4921 ggagtgatga ataagaaaaa caataagatc ttcgatgaca aagccattaa ggagaacaaa
     4981 ggggaaggat acaagaaaat cgtgtataag ctgctgcccg gcgcaaataa gatgctgcct
     5041 aaggtgttct tcagcgccaa gagtatcaaa ttctacaacc catccgagga catcctgcgg
     5101 attagaaatc actcaacaca tactaagaac gggagccccc agaagggata tgagaaattt
     5161 gagttcaaca tcgaggattg caggaagttt attgacttct acaagcagag catctccaaa
     5221 caccctgaat ggaaggattt tggcttccgg ttttccgaca cacagagata taactctatc
     5281 gacgagttct accgcgaggt ggaaaatcag gggtataagc tgacttttga gaacatttct
     5341 gaaagttaca tcgacagcgt ggtcaatcag ggaaagctgt acctgttcca gatctataac
     5401 aaagattttt cagcatacag caagggcaga ccaaacctgc atacactgta ctggaaggcc
     5461 ctgttcgatg agaggaatct gcaggacgtg gtctataaac tgaacggaga ggccgaactg
     5521 ttttaccgga agcagtctat tcctaagaaa atcactcacc cagctaagga ggccatcgct
     5581 aacaagaaca aggacaatcc taagaaagag agcgtgttcg aatacgatct gattaaggac
     5641 aagcggttca ccgaagataa gttctttttc cattgtccaa tcaccattaa cttcaagtca
     5701 agcggcgcta acaagttcaa cgacgagatc aatctgctgc tgaaggaaaa agcaaacgat
     5761 gtgcacatcc tgagcattga ccgaggagag cggcatctgg cctactatac cctggtggat
     5821 ggcaaaggga atatcattaa gcaggataca ttcaacatca ttggcaatga ccggatgaaa
     5881 accaactacc acgataaact ggctgcaatc gagaaggata gagactcagc taggaaggac
     5941 tggaagaaaa tcaacaacat taaggagatg aaggaaggct atctgagcca ggtggtccat
     6001 gagattgcaa agctggtcat cgaatacaat gccattgtgg tgttcgagga tctgaacttc
     6061 ggctttaaga gggggcgctt taaggtggaa aaacaggtct atcagaagct ggagaaaatg
     6121 ctgatcgaaa agctgaatta cctggtgttt aaagataacg agttcgacaa gaccggaggc
     6181 gtcctgagag cctaccagct gacagctccc tttgaaactt tcaagaaaat gggaaaacag
     6241 acaggcatca tctactatgt gccagccgga ttcacttcca agatctgccc cgtgaccggc
     6301 tttgtcaacc agctgtaccc taaatatgag tcagtgagca agtcccagga atttttcagc
     6361 aagttcgata agatctgtta taatctggac aaggggtact tcgagttttc cttcgattac
     6421 aagaacttcg gcgacaaggc cgctaagggg aaatggacca ttgcctcctt cggatctcgc
     6481 ctgatcaact ttcgaaattc cgataaaaac cacaattggg acactaggga ggtgtaccca
     6541 accaaggagc tggaaaagct gctgaaagac tactctatcg agtatggaca tggcgaatgc
     6601 atcaaggcag ccatctgtgg cgagagtgat aagaaatttt tcgccaagct gacctcagtg
     6661 ctgaatacaa tcctgcagat gcggaactca aagaccggga cagaactgga ctatctgatt
     6721 agccccgtgg ctgatgtcaa cggaaacttc ttcgacagca gacaggcacc caaaaatatg
     6781 cctcaggatg cagacgccaa cggggcctac cacatcgggc tgaagggact gatgctgctg
     6841 ggccggatca agaacaatca ggaggggaag aagctgaacc tggtcattaa gaacgaggaa
     6901 tacttcgagt ttgtccagaa tagaaataac gggtccggca gcgggtccgt taacatggtg
     6961 tttactctcg aggacttcgt gggcgactgg cggcagacag ctggctataa cctcgaccag
     7021 gtgctggagc agggcggtgt gtcctcgctt ttccagaacc tgggcgtctc cgtgacccca
     7081 atccagcgca tcgtcctgag cggcgagaac ggcctgaaga tcgacatcca cgtcatcatt
     7141 ccctacgagg gcctgagcgg ggaccaaatg ggccagatcg agaagatctt caaggttgtg
     7201 taccccgtgg acgatcacca cttcaaggtg atactgcact acgggacgct ggtcatcgac
     7261 ggtgtgacgc cgaacatgat tgactacttc ggccgtcctt acgagggcat tgccgtgttc
     7321 gacggcaaga agatcacggt gaccggcacc ctgtggaacg gcaataagat cattgacgag
     7381 cggctgatca acccggatgg ctcgctgttg ttccgcgtga ccatcaacgg ggtaaccggc
     7441 tggcgcctgt gcgagcgcat cctggccgga tacccctacg acgtgcccga ctacgcgtcc
     7501 atggtggcta gcccgaaaaa gaagaggaag gtctgacaat tggaaagatc caagagagac
     7561 gagtagagat tttttttttg ggattgatgt ttgtcgttct ttgagttgtc gtcgagttac
     7621 gccttttgta agaatgttcc gcaggagagg aggaggatgg gcatgagtga gggtgagagg
     7681 gcttgcccgc tttttttttt aaaaacgctg aagacgtggt tgtcaaacaa accccccata
     7741 gaaacgattt tgttacggtg cggtccagac gtcacttgaa tggctccgcg gaaaggccag
     7801 ggagggaagg ggggagggag gaaacatgaa acatgttgaa cggctcaaca gggtttgggg
     7861 gacaagagag gtagcgccct gatggactgc tccctcccct cctttccctc aatgtctcat
     7921 tcatccatgc ttcccccttc tctctctccc ctccgttcca tcccccgcgg gcgtggtagt
     7981 ggcgtgatgg gatccactaa aatgtacgtg taagaaaagc cggtgagctt acgcttttgt
     8041 gaaagtggga gtacgagtgt tgtgtgtgtg tgtagtggtt tcagacccca gacagaggcg
     8101 aagcagaaaa agcagacgat gaagacgacg aagaaatgag cagtctattt ttatcgtgga
     8161 aacagaagag gtgatatcgt ctcctgcagg catgcaagct gatccactag aggccatggc
     8221 ggccgcgagg gtggacttga gagtgtggaa gtgagtgagg gtagaagata gagtaaggat
     8281 ggcagacaag atgacgataa agaagacgtg gaagagaggg aaatacacgt acaaaggaag
     8341 aaaacaagaa aaggaaaaag aggaaaaaat aaacgcaggc ggaattactc actgttgaaa
     8401 tggtctctcg tcctgacacg gaagttgtac gaacaacacg catgcacaca acgtactctt
     8461 cttctgtgtt ctaaataaag actatttgct aagggagggt agtggcgatg gtggcgtaca
     8521 gtgacttaat ggggaggagc aaatcaatga tgcctttttt ggcatttttc tattgtctat
     8581 ctgcggtact acaattcgtt gttatacagt cacggatgga aggaagaagc agcagtagag
     8641 taggggataa accaatgaga taagggagct gatgtaacga gaccaggacg cctgaaatta
     8701 aagatagaaa aaccacaagt agagccagga gcgatgacca tcttccattt cgtttcttca
     8761 caacagtgct tttgctttct tcgcttctac tctctcctta ctggctacca tgacattgtt
     8821 cacttctttc atttctcctt ccctctctca cacacatccc accccttcga tttccttctg
     8881 cccttcttcc ctcggggtct agtcttattt tgattgaacc gatgcgtgaa ttggatcgag
     8941 tgacttgtat gacgggcaca cacacacatg acgacgacag gtgaggactt gaaggaccta
     9001 aggcatgaca cctaaaagga aggaaggccg aaagacgacg gcagaagtgg ggatctctga
     9061 aaccagatcc cttctcctct tcactccctc ctcctgatcc ctggccatca gtcctgctcc
     9121 tcggccacga agtgcacgca gttgccggcc gggtcgcgca gggcgaactc ccgcccccac
     9181 ggctgctcgc cgatctcggt catggccggc ccggaggcgt cccggaagtt cgtggacacg
     9241 acctccgacc actcggcgta cagctcgtcc aggccgcgca cccacaccca ggccagggtg
     9301 ttgtccggca ccacctggtc ctggaccgcg ctgatgaaca gggtcacgtc gtcccggacc
     9361 acaccggcga agtcgtcctc cacgaagtcc cgggagaacc cgagccggtc ggtccagaac
     9421 tcgaccgctc cggcgacgtc gcgcgcggtg agcaccggaa cggcactggt caacttggcc
     9481 atctgctcgg aggggaggat gaagggaagg agggaggggg gaggggcatt tatgacaatt
     9541 agaacttcag aggtctactc cttgtgtgta acgaggtgaa cgacgggtga aagtgttcgc
     9601 ctgacaggac tggtcgaaga tcggggggag ggggctacct ctatctctga gggcatcttt
     9661 ctgataaggt gccttcaacc gcaaccccga aaacccctgc cccccaccac accatggaca
     9721 catgcacgca cgacaaatag acagagcgat gccaacagga ttggttgtat agtatggttc
     9781 tggtaagata gagggattaa atgccctgct ccaggccgtg aggggcgaac tggcgcgctt
     9841 gtgcttctcc ttctgtactt aatctacatc cctgaccttg aactggatct tgacaaatat
     9901 agatccgaaa acccactgcc gcacgaactt accataattt aaaaagaggt tttagaggcg
     9961 aagagtggag acttctgtgt gaggtttgtg aaaaggtaag aaggagggga caggagatca
    10021 tgaatctata tcttaattaa gcacttctgc tgttttgaat ggggttagag cacaagtggg
    10081 atgcctggac tttcagggca tgcaagcaca cactacgacc acaacagaag tcaaaagggt
    10141 gtagatagag tttggcctct tgtctgatgg gcattcaccc ggcagtatcc tagttaaaac
    10201 aaagacacat gaagagatat aaagatcatc gcgtgtgccc tctagacggg cgggctatag
    10261 aggaaaggac aaaagaccgc caaaccgacg caggatgcgg acaggttcag tgcaccaagg
    10321 aggaggaaac gcccaaccac gaggtccaag gctggcagag aaataaacga ccccgtttaa
    10381 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
    10441 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
    10501 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
    10561 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
    10621 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
    10681 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
    10741 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
    10801 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
    10861 ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta
    10921 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
    10981 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
    11041 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
    11101 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
    11161 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg
    11221 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg
    11281 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc
    11341 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg
    11401 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg
    11461 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag
    11521 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat
    11581 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc
    11641 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc
    11701 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa
    11761 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac
    11821 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt
    11881 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc
    11941 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa
    12001 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca
    12061 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagc
//
