LOCUS       sequence-346569-       12116 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pNOC episomal plasmid harboring the humanized fnCas12a gene sequence
            tagged with Nlux and crRNA targeting the Nitrate reductase gene of 
            N. oceanica IMET1 with spacer 3.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12116)
  AUTHORS   Naduthodi MIS, Sudfeld C, Avitzigiannis EK, Trevisan N, van Lith E, 
            Alcaide Sancho J, D'Adamo S, Barbosa M, van der Oost J
  TITLE     Comprehensive Genome Engineering Toolbox for Microalgae 
            Nannochloropsis oceanica Based on CRISPR-Cas Systems.
  JOURNAL   ACS Synth Biol. 2021 Dec 17;10(12):3369-3378. doi: 
            10.1021/acssynbio.1c00329. Epub 2021 Nov 18.
  PUBMED    34793143
REFERENCE   2  (bases 1 to 12116)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.1c00329"; journalName: "ACS Synth Biol"; date: 
            "2021-12-17- 17"; volume: "10"; issue: "12"; pages: "3369-3378"
COMMENT     Sequence Label: pNOC_hfnCas12a-Nlux_crRNA NR3
FEATURES             Location/Qualifiers
     source          1..12116
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     ncRNA           complement(1494..1561)
                     /product="hepatitis delta virus self-cleaving ribozyme 
                     (Ferre-D'Amare and Scott, 2010)"
                     /label=HDV ribozyme
                     /ncRNA_class="ribozyme"
     ncRNA           complement(1623..1659)
                     /product="self-cleaving hammerhead ribozyme (Ferre-D'Amare 
                     and Scott, 2010)"
                     /label=hammerhead ribozyme
                     /ncRNA_class="hammerhead_ribozyme"
     CDS             2656..2667
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             2888..2908
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             7346..7372
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     regulatory      7374..7383
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             7388..7408
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     regulatory      8089..8098
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(8983..9357)
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     rep_origin      complement(10317..10905)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10397..10416)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(11076..11867,11868..11936))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11699..11718
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11937..12041)
                     /gene="bla"
                     /label=AmpR promoter
     misc_feature    join(12078..12116,1..465)
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
ORIGIN
        1 atataaatat ataaattaaa aatagaaagt aaaaaaagaa attaaagaaa aaatagtttt
       61 tgttttccga agatgtaaaa gactctaggg ggatcgccaa caaatactac cttttatctt
      121 gtacttcctg ctctcaggta ttaatgccga attgtttcat cttgtctgtg tagaagacca
      181 cacacgaaaa tcctgtgatt ttacatttta cttatcgtta atcgaatgta tatctattta
      241 atctgctttt cttgtctaat aaatatatat gtaaagtacg ctttttgttg aaatttttta
      301 aacctttgtt tatttttttt tcttcattcc gtaactcttc taccttcttt atttactttc
      361 taaaatccaa atacaaaaca taaaaataaa taaacacaga gtaaattccc aaattattcc
      421 atcattaaaa gatacgaggc gcgtgtaagt tacaggcaag cgatccgtct aagaaaccgg
      481 tgcaaagctg acgccctttt cgtcgtcgtt gctggtgtta aaattgcgcg tttctttttt
      541 ttccgtcttt gtcgtctttt gactttttta tcttcttttc tgtcttcttt tattcttctc
      601 ttcttcttca tcactccctc ttcatttttc ttcttctttt tcctcgtcta ttgtcctcgg
      661 gcattaccag ttgtgctgct cacgtgcgcg caagcggtga aagggtgaga ggatacacgg
      721 acaacaatca ttgaaaatta cgcttaaagc cggtgcctgg aaaagtccac gcgcagatgg
      781 cctttgcttg ccttgctgct gctgcagctg ctgctgctgt cgcctgtgat ggtaatggtt
      841 gttgttgtgg tcctgcttgc ttgctagttt gcattgcgtt tgcggttgcc ttatgaccga
      901 ctccgtgtga ttgatcctta aacatatttt atcattgatg tggattgtgt gacgacggca
      961 aaaaagaatt actttacaca tgactatcac acctgctccc ctcccttctc ctttcaatcg
     1021 gtcaacttcc caaccgctcc tcgaaaatat ttcagttgaa tcctctacta cgtacccctt
     1081 acttaaattt actttctctc tccccatcaa tgcgccgttg ttctgaagtt gtattattga
     1141 tgggagatga ttggcactgc accagacctg gccaaaagcc accacaggtg gccacatact
     1201 cttgcatggg tatactctac cgaggcaaag attaaagtgc acgtcccctg cccctctctc
     1261 tccctacttc cttcagtctc agtggttccg tgcccgttca gcactacatc gccctgcccg
     1321 ctgttccttc tcaaacagcc acccatccca acccacatac acagaaacaa cctttcctac
     1381 acattttccc aagtcttctc ttcactattc tacactaata caccaccgtg atatgccggt
     1441 tatctagatg tcctttctcc tttctcatcc tttctttccc atggtaccca ggagtcccat
     1501 tcgccatgcc gaagcatgtt gcccagccgg cgccagcgag gaggctggga ccatgccggc
     1561 catggaggca ggggccgacg agactgatct acaacagtag aaattattta aagttcttag
     1621 acgacgagct tactcgtttc gtcctcacgg actcatcagg tctaagaaga gccaaggcct
     1681 gctcttcatc gattctgtgg atggagggag ggaaggaggg ggggagtgag tgagtcgaga
     1741 gaacgacgac tggggcaaag agaggatgac gccactgcaa caaaagacag ggaagaatag
     1801 aggctgttgg aaaacaggaa cacagtgaag aatagaggct gcagggtaac aggcacattt
     1861 atcgcagaga ggcgggcggc aggggcgata cgtaaagaga cccctaaact tctccgcgtc
     1921 atccgttcac gctacctcct tccctccctt tactcccatt gatcaccaag gcagcgcccg
     1981 cagccaccag cagccaccgt cctcagtgtc acagctagac gcgtccccat caggcagcag
     2041 accaccagaa tatgcgcttt cgcttcccat catatccctc cctcctcccc ttctattcct
     2101 catcatgcat catttctttc tccttgctgc atgcccctct tcttaccttg gctaagatct
     2161 accggaggaa gaagtcttca acgatagata aatgcctgta tcgtgatggt tgtttgaatg
     2221 gtggaagtga gccaggtatt gagaacgagg ggcgcaaggg tgcgtggaag aaggcaggga
     2281 gaggcgaggc gaaggagaag ggcatgtggt gggaacgaag cctccacacc acagccaccc
     2341 atgcttttct ctgttcgagt tcccgggttc tactctcaca cactacacgt gcacagaaaa
     2401 cattcacgca caacagagat acagagggat ccgtcgagat ggtctacacg tcctaatgtc
     2461 ccctgctttc gaagcccaga gccatcgctt tctgttttta tccattcgca acacatcctg
     2521 ttctcgcgac ctctcctctt cctggccacc atcgcttctc cgtgccatgc cctgtacatc
     2581 cttccctccc tcgttgtcta ccacactaat catgcgctct gcagcagaca ctcggctact
     2641 atttgtgtcg tacggattga ggggagggag gggaagggag gcaagaagta aatcggtggt
     2701 caacaacaga cgcggtctcc ccaccctccc tcattccttt cccctcctat aatgagcgaa
     2761 gaaagccatg gacaccgagt cacccactct cctaatccac caccaccagg gcactaacgg
     2821 actcacgctc accaaactcg gtaacaatat ccactgcaca cagatacaca cgcaaccctc
     2881 gagcatgccc aagaaaaagc ggaaggtgag catctaccag gagttcgtca acaagtattc
     2941 actgagtaag acactgcggt tcgagctgat cccacagggc aagacactgg agaacatcaa
     3001 ggcccgaggc ctgattctgg acgatgagaa gcgggcaaaa gactataaga aagccaagca
     3061 gatcattgat aaataccacc agttctttat cgaggaaatt ctgagctccg tgtgcatcag
     3121 tgaggatctg ctgcagaatt actcagacgt gtacttcaag ctgaagaaga gcgacgatga
     3181 caacctgcag aaggacttca agtccgccaa ggacaccatc aagaaacaga ttagcgagta
     3241 catcaaggac tccgaaaagt ttaaaaatct gttcaaccag aatctgatcg atgctaagaa
     3301 aggccaggag tccgacctga tcctgtggct gaaacagtct aaggacaatg ggattgaact
     3361 gttcaaggct aactccgata tcactgatat tgacgaggca ctggaaatca tcaagagctt
     3421 caagggatgg accacatact ttaaaggctt ccacgagaac cgcaagaacg tgtactccag
     3481 caacgacatt cctacctcca tcatctaccg aatcgtcgat gacaatctgc caaagttcct
     3541 ggagaacaag gccaaatatg aatctctgaa ggacaaagct cccgaggcaa ttaattacga
     3601 acagatcaag aaagatctgg ctgaggaact gacattcgat atcgactata agactagcga
     3661 ggtgaaccag agggtctttt ccctggacga ggtgtttgaa atcgccaatt tcaacaatta
     3721 cctgaaccag tccggcatta ctaaattcaa taccatcatt ggcgggaagt ttgtgaacgg
     3781 ggagaatacc aagcgcaagg gaattaacga atacatcaat ctgtatagcc agcagatcaa
     3841 cgacaaaact ctgaagaaat acaagatgtc tgtgctgttc aaacagatcc tgagtgatac
     3901 cgagtccaag tcttttgtca ttgataaact ggaagatgac tcagacgtgg tcactaccat
     3961 gcagagcttt tatgagcaga tcgccgcttt caagacagtg gaggaaaaat ctattaagga
     4021 aactctgagt ctgctgttcg atgacctgaa agcccagaag ctggacctga gtaagatcta
     4081 cttcaaaaac gataagagtc tgacagacct gtcacagcag gtgtttgatg actattccgt
     4141 gattgggacc gccgtcctgg agtacattac acagcagatc gctccaaaga acctggataa
     4201 tccctctaag aaagagcagg aactgatcgc taagaaaacc gagaaggcaa aatatctgag
     4261 tctggaaaca attaagctgg cactggagga gttcaacaag cacagggata ttgacaaaca
     4321 gtgccgcttt gaggaaatcc tggccaactt cgcagccatc cccatgattt ttgatgagat
     4381 cgcccagaac aaagacaatc tggctcagat cagtattaag taccagaacc agggcaagaa
     4441 agacctgctg caggcttcag cagaagatga cgtgaaagcc atcaaggatc tgctggacca
     4501 gaccaacaat ctgctgcaca agctgaaaat cttccatatt agtcagtcag aggataaggc
     4561 taatatcctg gataaagacg aacacttcta cctggtgttc gaggaatgtt acttcgagct
     4621 ggcaaacatt gtccccctgt ataacaagat taggaactac atcacacaga agccttactc
     4681 tgacgagaag tttaaactga acttcgaaaa tagtaccctg gccaacgggt gggataagaa
     4741 caaggagcct gacaacacag ctatcctgtt catcaaggat gacaagtact atctgggagt
     4801 gatgaataag aaaaacaata agatcttcga tgacaaagcc attaaggaga acaaagggga
     4861 aggatacaag aaaatcgtgt ataagctgct gcccggcgca aataagatgc tgcctaaggt
     4921 gttcttcagc gccaagagta tcaaattcta caacccatcc gaggacatcc tgcggattag
     4981 aaatcactca acacatacta agaacgggag cccccagaag ggatatgaga aatttgagtt
     5041 caacatcgag gattgcagga agtttattga cttctacaag cagagcatct ccaaacaccc
     5101 tgaatggaag gattttggct tccggttttc cgacacacag agatataact ctatcgacga
     5161 gttctaccgc gaggtggaaa atcaggggta taagctgact tttgagaaca tttctgaaag
     5221 ttacatcgac agcgtggtca atcagggaaa gctgtacctg ttccagatct ataacaaaga
     5281 tttttcagca tacagcaagg gcagaccaaa cctgcataca ctgtactgga aggccctgtt
     5341 cgatgagagg aatctgcagg acgtggtcta taaactgaac ggagaggccg aactgtttta
     5401 ccggaagcag tctattccta agaaaatcac tcacccagct aaggaggcca tcgctaacaa
     5461 gaacaaggac aatcctaaga aagagagcgt gttcgaatac gatctgatta aggacaagcg
     5521 gttcaccgaa gataagttct ttttccattg tccaatcacc attaacttca agtcaagcgg
     5581 cgctaacaag ttcaacgacg agatcaatct gctgctgaag gaaaaagcaa acgatgtgca
     5641 catcctgagc attgaccgag gagagcggca tctggcctac tataccctgg tggatggcaa
     5701 agggaatatc attaagcagg atacattcaa catcattggc aatgaccgga tgaaaaccaa
     5761 ctaccacgat aaactggctg caatcgagaa ggatagagac tcagctagga aggactggaa
     5821 gaaaatcaac aacattaagg agatgaagga aggctatctg agccaggtgg tccatgagat
     5881 tgcaaagctg gtcatcgaat acaatgccat tgtggtgttc gaggatctga acttcggctt
     5941 taagaggggg cgctttaagg tggaaaaaca ggtctatcag aagctggaga aaatgctgat
     6001 cgaaaagctg aattacctgg tgtttaaaga taacgagttc gacaagaccg gaggcgtcct
     6061 gagagcctac cagctgacag ctccctttga aactttcaag aaaatgggaa aacagacagg
     6121 catcatctac tatgtgccag ccggattcac ttccaagatc tgccccgtga ccggctttgt
     6181 caaccagctg taccctaaat atgagtcagt gagcaagtcc caggaatttt tcagcaagtt
     6241 cgataagatc tgttataatc tggacaaggg gtacttcgag ttttccttcg attacaagaa
     6301 cttcggcgac aaggccgcta aggggaaatg gaccattgcc tccttcggat ctcgcctgat
     6361 caactttcga aattccgata aaaaccacaa ttgggacact agggaggtgt acccaaccaa
     6421 ggagctggaa aagctgctga aagactactc tatcgagtat ggacatggcg aatgcatcaa
     6481 ggcagccatc tgtggcgaga gtgataagaa atttttcgcc aagctgacct cagtgctgaa
     6541 tacaatcctg cagatgcgga actcaaagac cgggacagaa ctggactatc tgattagccc
     6601 cgtggctgat gtcaacggaa acttcttcga cagcagacag gcacccaaaa atatgcctca
     6661 ggatgcagac gccaacgggg cctaccacat cgggctgaag ggactgatgc tgctgggccg
     6721 gatcaagaac aatcaggagg ggaagaagct gaacctggtc attaagaacg aggaatactt
     6781 cgagtttgtc cagaatagaa ataacgggtc cggcagcggg tccgttaaca tggtgtttac
     6841 tctcgaggac ttcgtgggcg actggcggca gacagctggc tataacctcg accaggtgct
     6901 ggagcagggc ggtgtgtcct cgcttttcca gaacctgggc gtctccgtga ccccaatcca
     6961 gcgcatcgtc ctgagcggcg agaacggcct gaagatcgac atccacgtca tcattcccta
     7021 cgagggcctg agcggggacc aaatgggcca gatcgagaag atcttcaagg ttgtgtaccc
     7081 cgtggacgat caccacttca aggtgatact gcactacggg acgctggtca tcgacggtgt
     7141 gacgccgaac atgattgact acttcggccg tccttacgag ggcattgccg tgttcgacgg
     7201 caagaagatc acggtgaccg gcaccctgtg gaacggcaat aagatcattg acgagcggct
     7261 gatcaacccg gatggctcgc tgttgttccg cgtgaccatc aacggggtaa ccggctggcg
     7321 cctgtgcgag cgcatcctgg ccggataccc ctacgacgtg cccgactacg cgtccatggt
     7381 ggctagcccg aaaaagaaga ggaaggtctg acaattggaa agatccaaga gagacgagta
     7441 gagatttttt ttttgggatt gatgtttgtc gttctttgag ttgtcgtcga gttacgcctt
     7501 ttgtaagaat gttccgcagg agaggaggag gatgggcatg agtgagggtg agagggcttg
     7561 cccgcttttt tttttaaaaa cgctgaagac gtggttgtca aacaaacccc ccatagaaac
     7621 gattttgtta cggtgcggtc cagacgtcac ttgaatggct ccgcggaaag gccagggagg
     7681 gaagggggga gggaggaaac atgaaacatg ttgaacggct caacagggtt tgggggacaa
     7741 gagaggtagc gccctgatgg actgctccct cccctccttt ccctcaatgt ctcattcatc
     7801 catgcttccc ccttctctct ctcccctccg ttccatcccc cgcgggcgtg gtagtggcgt
     7861 gatgggatcc actaaaatgt acgtgtaaga aaagccggtg agcttacgct tttgtgaaag
     7921 tgggagtacg agtgttgtgt gtgtgtgtag tggtttcaga ccccagacag aggcgaagca
     7981 gaaaaagcag acgatgaaga cgacgaagaa atgagcagtc tatttttatc gtggaaacag
     8041 aagaggtgat atcgtctcct gcaggcatgc aagctgatcc actagaggcc atggcggccg
     8101 cgagggtgga cttgagagtg tggaagtgag tgagggtaga agatagagta aggatggcag
     8161 acaagatgac gataaagaag acgtggaaga gagggaaata cacgtacaaa ggaagaaaac
     8221 aagaaaagga aaaagaggaa aaaataaacg caggcggaat tactcactgt tgaaatggtc
     8281 tctcgtcctg acacggaagt tgtacgaaca acacgcatgc acacaacgta ctcttcttct
     8341 gtgttctaaa taaagactat ttgctaaggg agggtagtgg cgatggtggc gtacagtgac
     8401 ttaatgggga ggagcaaatc aatgatgcct tttttggcat ttttctattg tctatctgcg
     8461 gtactacaat tcgttgttat acagtcacgg atggaaggaa gaagcagcag tagagtaggg
     8521 gataaaccaa tgagataagg gagctgatgt aacgagacca ggacgcctga aattaaagat
     8581 agaaaaacca caagtagagc caggagcgat gaccatcttc catttcgttt cttcacaaca
     8641 gtgcttttgc tttcttcgct tctactctct ccttactggc taccatgaca ttgttcactt
     8701 ctttcatttc tccttccctc tctcacacac atcccacccc ttcgatttcc ttctgccctt
     8761 cttccctcgg ggtctagtct tattttgatt gaaccgatgc gtgaattgga tcgagtgact
     8821 tgtatgacgg gcacacacac acatgacgac gacaggtgag gacttgaagg acctaaggca
     8881 tgacacctaa aaggaaggaa ggccgaaaga cgacggcaga agtggggatc tctgaaacca
     8941 gatcccttct cctcttcact ccctcctcct gatccctggc catcagtcct gctcctcggc
     9001 cacgaagtgc acgcagttgc cggccgggtc gcgcagggcg aactcccgcc cccacggctg
     9061 ctcgccgatc tcggtcatgg ccggcccgga ggcgtcccgg aagttcgtgg acacgacctc
     9121 cgaccactcg gcgtacagct cgtccaggcc gcgcacccac acccaggcca gggtgttgtc
     9181 cggcaccacc tggtcctgga ccgcgctgat gaacagggtc acgtcgtccc ggaccacacc
     9241 ggcgaagtcg tcctccacga agtcccggga gaacccgagc cggtcggtcc agaactcgac
     9301 cgctccggcg acgtcgcgcg cggtgagcac cggaacggca ctggtcaact tggccatctg
     9361 ctcggagggg aggatgaagg gaaggaggga ggggggaggg gcatttatga caattagaac
     9421 ttcagaggtc tactccttgt gtgtaacgag gtgaacgacg ggtgaaagtg ttcgcctgac
     9481 aggactggtc gaagatcggg gggagggggc tacctctatc tctgagggca tctttctgat
     9541 aaggtgcctt caaccgcaac cccgaaaacc cctgcccccc accacaccat ggacacatgc
     9601 acgcacgaca aatagacaga gcgatgccaa caggattggt tgtatagtat ggttctggta
     9661 agatagaggg attaaatgcc ctgctccagg ccgtgagggg cgaactggcg cgcttgtgct
     9721 tctccttctg tacttaatct acatccctga ccttgaactg gatcttgaca aatatagatc
     9781 cgaaaaccca ctgccgcacg aacttaccat aatttaaaaa gaggttttag aggcgaagag
     9841 tggagacttc tgtgtgaggt ttgtgaaaag gtaagaagga ggggacagga gatcatgaat
     9901 ctatatctta attaagcact tctgctgttt tgaatggggt tagagcacaa gtgggatgcc
     9961 tggactttca gggcatgcaa gcacacacta cgaccacaac agaagtcaaa agggtgtaga
    10021 tagagtttgg cctcttgtct gatgggcatt cacccggcag tatcctagtt aaaacaaaga
    10081 cacatgaaga gatataaaga tcatcgcgtg tgccctctag acgggcgggc tatagaggaa
    10141 aggacaaaag accgccaaac cgacgcagga tgcggacagg ttcagtgcac caaggaggag
    10201 gaaacgccca accacgaggt ccaaggctgg cagagaaata aacgaccccg tttaaacatg
    10261 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
    10321 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
    10381 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
    10441 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
    10501 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
    10561 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
    10621 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
    10681 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
    10741 tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc
    10801 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
    10861 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
    10921 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg
    10981 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca
    11041 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca
    11101 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag
    11161 ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac
    11221 ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc
    11281 agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct
    11341 agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc
    11401 gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg
    11461 cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc
    11521 gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat
    11581 tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag
    11641 tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat
    11701 aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg
    11761 cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca
    11821 cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga
    11881 aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc
    11941 ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata
    12001 tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg
    12061 ccacctgggt ccttttcatc acgtgctata aaaataatta taatttaaat ttttta
//
