LOCUS       sequence-346570-       12088 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pNOC episomal plasmid harboring the humanized fnCas12a gene sequence
            tagged with Nlux under the control of Ribi promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12088)
  AUTHORS   Naduthodi MIS, Sudfeld C, Avitzigiannis EK, Trevisan N, van Lith E, 
            Alcaide Sancho J, D'Adamo S, Barbosa M, van der Oost J
  TITLE     Comprehensive Genome Engineering Toolbox for Microalgae 
            Nannochloropsis oceanica Based on CRISPR-Cas Systems.
  JOURNAL   ACS Synth Biol. 2021 Dec 17;10(12):3369-3378. doi: 
            10.1021/acssynbio.1c00329. Epub 2021 Nov 18.
  PUBMED    34793143
REFERENCE   2  (bases 1 to 12088)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.1c00329"; journalName: "ACS Synth Biol"; date: 
            "2021-12-17- 17"; volume: "10"; issue: "12"; pages: "3369-3378"
COMMENT     Sequence Label: pNOC_hfnCas12a-Nlux
FEATURES             Location/Qualifiers
     source          1..12088
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..504
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
     ncRNA           complement(1533..1600)
                     /product="hepatitis delta virus self-cleaving ribozyme 
                     (Ferre-D'Amare and Scott, 2010)"
                     /label=HDV ribozyme
                     /ncRNA_class="ribozyme"
     ncRNA           complement(1658..1694)
                     /product="self-cleaving hammerhead ribozyme (Ferre-D'Amare 
                     and Scott, 2010)"
                     /label=hammerhead ribozyme
                     /ncRNA_class="hammerhead_ribozyme"
     CDS             2669..2680
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             2901..2921
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             7359..7385
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     regulatory      7387..7396
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             7401..7421
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     regulatory      8100..8109
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(8994..9368)
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     rep_origin      complement(10328..10916)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10408..10427)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(11087..11878,11879..11947))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11710..11729
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11948..12052)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 atcacgtgct ataaaaataa ttataattta aattttttaa tataaatata taaattaaaa
       61 atagaaagta aaaaaagaaa ttaaagaaaa aatagttttt gttttccgaa gatgtaaaag
      121 actctagggg gatcgccaac aaatactacc ttttatcttg tacttcctgc tctcaggtat
      181 taatgccgaa ttgtttcatc ttgtctgtgt agaagaccac acacgaaaat cctgtgattt
      241 tacattttac ttatcgttaa tcgaatgtat atctatttaa tctgcttttc ttgtctaata
      301 aatatatatg taaagtacgc tttttgttga aattttttaa acctttgttt attttttttt
      361 cttcattccg taactcttct accttcttta tttactttct aaaatccaaa tacaaaacat
      421 aaaaataaat aaacacagag taaattccca aattattcca tcattaaaag atacgaggcg
      481 cgtgtaagtt acaggcaagc gatccgtcta agaaaccggt gcaaagctga cgcccttttc
      541 gtcgtcgttg ctggtgttaa aattgcgcgt ttcttttttt tccgtctttg tcgtcttttg
      601 acttttttat cttcttttct gtcttctttt attcttctct tcttcttcat cactccctct
      661 tcatttttct tcttcttttt cctcgtctat tgtcctcggg cattaccagt tgtgctgctc
      721 acgtgcgcgc aagcggtgaa agggtgagag gatacacgga caacaatcat tgaaaattac
      781 gcttaaagcc ggtgcctgga aaagtccacg cgcagatggc ctttgcttgc cttgctgctg
      841 ctgcagctgc tgctgctgtc gcctgtgatg gtaatggttg ttgttgtggt cctgcttgct
      901 tgctagtttg cattgcgttt gcggttgcct tatgaccgac tccgtgtgat tgatccttaa
      961 acatatttta tcattgatgt ggattgtgtg acgacggcaa aaaagaatta ctttacacat
     1021 gactatcaca cctgctcccc tcccttctcc tttcaatcgg tcaacttccc aaccgctcct
     1081 cgaaaatatt tcagttgaat cctctactac gtacccctta cttaaattta ctttctctct
     1141 ccccatcaat gcgccgttgt tctgaagttg tattattgat gggagatgat tggcactgca
     1201 ccagacctgg ccaaaagcca ccacaggtgg ccacatactc ttgcatgggt atactctacc
     1261 gaggcaaaga ttaaagtgca cgtcccctgc ccctctctct ccctacttcc ttcagtctca
     1321 gtggttccgt gcccgttcag cactacatcg ccctgcccgc tgttccttct caaacagcca
     1381 cccatcccaa cccacataca cagaaacaac ctttcctaca cattttccca agtcttctct
     1441 tcactattct acactaatac accaccgtga tatgccggtt atctagatgt cctttctcct
     1501 ttctcatcct ttctttccca tggtacccag gagtcccatt cgccatgccg aagcatgttg
     1561 cccagccggc gccagcgagg aggctgggac catgccggcc ggtacccttg aggaggggga
     1621 tactcgtgcc aatctacaac agtagaaatt gctcttcgac gagcttactc gtttcgtcct
     1681 cacggactca tcagaatttc atcgattctg tggatggagg gagggaagga gggggggagt
     1741 gagtgagtcg agagaacgac gactggggca aagagaggat gacgccactg caacaaaaga
     1801 cagggaagaa tagaggctgt tggaaaacag gaacacagtg aagaatagag gctgcagggt
     1861 aacaggcaca tttatcgcag agaggcgggc ggcaggggcg atacgtaaag agacccctaa
     1921 acttctccgc gtcatccgtt cacgctacct ccttccctcc ctttactccc attgatcacc
     1981 aaggcagcgc ccgcagccac cagcagccac cgtcctcagt gtcacagcta gacgcgtccc
     2041 catcaggcag cagaccacca gaatatgcgc tttcgcttcc catcatatcc ctccctcctc
     2101 cccttctatt cctcatcatg catcatttct ttctccttgc tgcatgcccc tcttcttacc
     2161 ttggctaaga tctaccggag gaagaagtct tcaacgatag ataaatgcct gtatcgtgat
     2221 ggttgtttga atggtggaag tgagccaggt attgagaacg aggggcgcaa gggtgcgtgg
     2281 aagaaggcag ggagaggcga ggcgaaggag aagggcatgt ggtgggaacg aagcctccac
     2341 accacagcca cccatgcttt tctctgttcg agttcccggg ttctactctc acacactaca
     2401 cgtgcacaga aaacattcac gcacaacaga gatacagagg gatccgtcga gatggtctac
     2461 acgtcctaat gtcccctgct ttcgaagccc agagccatcg ctttctgttt ttatccattc
     2521 gcaacacatc ctgttctcgc gacctctcct cttcctggcc accatcgctt ctccgtgcca
     2581 tgccctgtac atccttccct ccctcgttgt ctaccacact aatcatgcgc tctgcagcag
     2641 acactcggct actatttgtg tcgtacggat tgaggggagg gaggggaagg gaggcaagaa
     2701 gtaaatcggt ggtcaacaac agacgcggtc tccccaccct ccctcattcc tttcccctcc
     2761 tataatgagc gaagaaagcc atggacaccg agtcacccac tctcctaatc caccaccacc
     2821 agggcactaa cggactcacg ctcaccaaac tcggtaacaa tatccactgc acacagatac
     2881 acacgcaacc ctcgagcatg cccaagaaaa agcggaaggt gagcatctac caggagttcg
     2941 tcaacaagta ttcactgagt aagacactgc ggttcgagct gatcccacag ggcaagacac
     3001 tggagaacat caaggcccga ggcctgattc tggacgatga gaagcgggca aaagactata
     3061 agaaagccaa gcagatcatt gataaatacc accagttctt tatcgaggaa attctgagct
     3121 ccgtgtgcat cagtgaggat ctgctgcaga attactcaga cgtgtacttc aagctgaaga
     3181 agagcgacga tgacaacctg cagaaggact tcaagtccgc caaggacacc atcaagaaac
     3241 agattagcga gtacatcaag gactccgaaa agtttaaaaa tctgttcaac cagaatctga
     3301 tcgatgctaa gaaaggccag gagtccgacc tgatcctgtg gctgaaacag tctaaggaca
     3361 atgggattga actgttcaag gctaactccg atatcactga tattgacgag gcactggaaa
     3421 tcatcaagag cttcaaggga tggaccacat actttaaagg cttccacgag aaccgcaaga
     3481 acgtgtactc cagcaacgac attcctacct ccatcatcta ccgaatcgtc gatgacaatc
     3541 tgccaaagtt cctggagaac aaggccaaat atgaatctct gaaggacaaa gctcccgagg
     3601 caattaatta cgaacagatc aagaaagatc tggctgagga actgacattc gatatcgact
     3661 ataagactag cgaggtgaac cagagggtct tttccctgga cgaggtgttt gaaatcgcca
     3721 atttcaacaa ttacctgaac cagtccggca ttactaaatt caataccatc attggcggga
     3781 agtttgtgaa cggggagaat accaagcgca agggaattaa cgaatacatc aatctgtata
     3841 gccagcagat caacgacaaa actctgaaga aatacaagat gtctgtgctg ttcaaacaga
     3901 tcctgagtga taccgagtcc aagtcttttg tcattgataa actggaagat gactcagacg
     3961 tggtcactac catgcagagc ttttatgagc agatcgccgc tttcaagaca gtggaggaaa
     4021 aatctattaa ggaaactctg agtctgctgt tcgatgacct gaaagcccag aagctggacc
     4081 tgagtaagat ctacttcaaa aacgataaga gtctgacaga cctgtcacag caggtgtttg
     4141 atgactattc cgtgattggg accgccgtcc tggagtacat tacacagcag atcgctccaa
     4201 agaacctgga taatccctct aagaaagagc aggaactgat cgctaagaaa accgagaagg
     4261 caaaatatct gagtctggaa acaattaagc tggcactgga ggagttcaac aagcacaggg
     4321 atattgacaa acagtgccgc tttgaggaaa tcctggccaa cttcgcagcc atccccatga
     4381 tttttgatga gatcgcccag aacaaagaca atctggctca gatcagtatt aagtaccaga
     4441 accagggcaa gaaagacctg ctgcaggctt cagcagaaga tgacgtgaaa gccatcaagg
     4501 atctgctgga ccagaccaac aatctgctgc acaagctgaa aatcttccat attagtcagt
     4561 cagaggataa ggctaatatc ctggataaag acgaacactt ctacctggtg ttcgaggaat
     4621 gttacttcga gctggcaaac attgtccccc tgtataacaa gattaggaac tacatcacac
     4681 agaagcctta ctctgacgag aagtttaaac tgaacttcga aaatagtacc ctggccaacg
     4741 ggtgggataa gaacaaggag cctgacaaca cagctatcct gttcatcaag gatgacaagt
     4801 actatctggg agtgatgaat aagaaaaaca ataagatctt cgatgacaaa gccattaagg
     4861 agaacaaagg ggaaggatac aagaaaatcg tgtataagct gctgcccggc gcaaataaga
     4921 tgctgcctaa ggtgttcttc agcgccaaga gtatcaaatt ctacaaccca tccgaggaca
     4981 tcctgcggat tagaaatcac tcaacacata ctaagaacgg gagcccccag aagggatatg
     5041 agaaatttga gttcaacatc gaggattgca ggaagtttat tgacttctac aagcagagca
     5101 tctccaaaca ccctgaatgg aaggattttg gcttccggtt ttccgacaca cagagatata
     5161 actctatcga cgagttctac cgcgaggtgg aaaatcaggg gtataagctg acttttgaga
     5221 acatttctga aagttacatc gacagcgtgg tcaatcaggg aaagctgtac ctgttccaga
     5281 tctataacaa agatttttca gcatacagca agggcagacc aaacctgcat acactgtact
     5341 ggaaggccct gttcgatgag aggaatctgc aggacgtggt ctataaactg aacggagagg
     5401 ccgaactgtt ttaccggaag cagtctattc ctaagaaaat cactcaccca gctaaggagg
     5461 ccatcgctaa caagaacaag gacaatccta agaaagagag cgtgttcgaa tacgatctga
     5521 ttaaggacaa gcggttcacc gaagataagt tctttttcca ttgtccaatc accattaact
     5581 tcaagtcaag cggcgctaac aagttcaacg acgagatcaa tctgctgctg aaggaaaaag
     5641 caaacgatgt gcacatcctg agcattgacc gaggagagcg gcatctggcc tactataccc
     5701 tggtggatgg caaagggaat atcattaagc aggatacatt caacatcatt ggcaatgacc
     5761 ggatgaaaac caactaccac gataaactgg ctgcaatcga gaaggataga gactcagcta
     5821 ggaaggactg gaagaaaatc aacaacatta aggagatgaa ggaaggctat ctgagccagg
     5881 tggtccatga gattgcaaag ctggtcatcg aatacaatgc cattgtggtg ttcgaggatc
     5941 tgaacttcgg ctttaagagg gggcgcttta aggtggaaaa acaggtctat cagaagctgg
     6001 agaaaatgct gatcgaaaag ctgaattacc tggtgtttaa agataacgag ttcgacaaga
     6061 ccggaggcgt cctgagagcc taccagctga cagctccctt tgaaactttc aagaaaatgg
     6121 gaaaacagac aggcatcatc tactatgtgc cagccggatt cacttccaag atctgccccg
     6181 tgaccggctt tgtcaaccag ctgtacccta aatatgagtc agtgagcaag tcccaggaat
     6241 ttttcagcaa gttcgataag atctgttata atctggacaa ggggtacttc gagttttcct
     6301 tcgattacaa gaacttcggc gacaaggccg ctaaggggaa atggaccatt gcctccttcg
     6361 gatctcgcct gatcaacttt cgaaattccg ataaaaacca caattgggac actagggagg
     6421 tgtacccaac caaggagctg gaaaagctgc tgaaagacta ctctatcgag tatggacatg
     6481 gcgaatgcat caaggcagcc atctgtggcg agagtgataa gaaatttttc gccaagctga
     6541 cctcagtgct gaatacaatc ctgcagatgc ggaactcaaa gaccgggaca gaactggact
     6601 atctgattag ccccgtggct gatgtcaacg gaaacttctt cgacagcaga caggcaccca
     6661 aaaatatgcc tcaggatgca gacgccaacg gggcctacca catcgggctg aagggactga
     6721 tgctgctggg ccggatcaag aacaatcagg aggggaagaa gctgaacctg gtcattaaga
     6781 acgaggaata cttcgagttt gtccagaata gaaataacgg gtccggcagc gggtccgtta
     6841 acatggtgtt tactctcgag gacttcgtgg gcgactggcg gcagacagct ggctataacc
     6901 tcgaccaggt gctggagcag ggcggtgtgt cctcgctttt ccagaacctg ggcgtctccg
     6961 tgaccccaat ccagcgcatc gtcctgagcg gcgagaacgg cctgaagatc gacatccacg
     7021 tcatcattcc ctacgagggc ctgagcgggg accaaatggg ccagatcgag aagatcttca
     7081 aggttgtgta ccccgtggac gatcaccact tcaaggtgat actgcactac gggacgctgg
     7141 tcatcgacgg tgtgacgccg aacatgattg actacttcgg ccgtccttac gagggcattg
     7201 ccgtgttcga cggcaagaag atcacggtga ccggcaccct gtggaacggc aataagatca
     7261 ttgacgagcg gctgatcaac ccggatggct cgctgttgtt ccgcgtgacc atcaacgggg
     7321 taaccggctg gcgcctgtgc gagcgcatcc tggccggata cccctacgac gtgcccgact
     7381 acgcgtccat ggtggctagc ccgaaaaaga agaggaaggt ctgacaattg gaaagatcca
     7441 agagagacga gtagagattt tttttttggg attgatgttt gtcgttcttt gagttgtcgt
     7501 cgagttacgc cttttgtaag aatgttccgc aggagaggag gaggatgggc atgagtgagg
     7561 gtgagagggc ttgcccgctt ttttttttaa aaacgctgaa gacgtggttg tcaaacaaac
     7621 cccccataga aacgattttg ttacggtgcg gtccagacgt cacttgaatg gctccgcgga
     7681 aaggccaggg agggaagggg ggagggagga aacatgaaac atgttgaacg gctcaacagg
     7741 gtttggggga caagagaggt agcgccctga tggactgctc cctcccctcc tttccctcaa
     7801 tgtctcattc atccatgctt cccccttctc tctctcccct ccgttccatc ccccgcgggc
     7861 gtggtagtgg cgtgatggga tccactaaaa tgtacgtgta agaaaagccg gtgagcttac
     7921 gcttttgtga aagtgggagt acgagtgttg tgtgtgtgta gtggtttcag accccagaca
     7981 gaggcgaagc agaaaaagca gacgatgaag acgacgaaga aatgagcagt ctatttttat
     8041 cgtggaaaca gaagaggtga tatcgtctcc tgcaggcatg caagctgatc cactagaggc
     8101 catggcggcc gcgagggtgg acttgagagt gtggaagtga gtgagggtag aagatagagt
     8161 aaggatggca gacaagatga cgataaagaa gacgtggaag agagggaaat acacgtacaa
     8221 aggaagaaaa caagaaaagg aaaaagagga aaaaataaac gcaggcggaa ttactcactg
     8281 ttgaaatggt ctctcgtcct gacacggaag ttgtacgaac aacacgcatg cacacaacgt
     8341 actcttcttc tgtgttctaa ataaagacta tttgctaagg gagggtagtg gcgatggtgg
     8401 cgtacagtga cttaatgggg aggagcaaat caatgatgcc ttttttggca tttttctatt
     8461 gtctatctgc ggtactacaa ttcgttgtta tacagtcacg gatggaagga agaagcagca
     8521 gtagagtagg ggataaacca atgagataag ggagctgatg taacgagacc aggacgcctg
     8581 aaattaaaga tagaaaaacc acaagtagag ccaggagcga tgaccatctt ccatttcgtt
     8641 tcttcacaac agtgcttttg ctttcttcgc ttctactctc tccttactgg ctaccatgac
     8701 attgttcact tctttcattt ctccttccct ctctcacaca catcccaccc cttcgatttc
     8761 cttctgccct tcttccctcg gggtctagtc ttattttgat tgaaccgatg cgtgaattgg
     8821 atcgagtgac ttgtatgacg ggcacacaca cacatgacga cgacaggtga ggacttgaag
     8881 gacctaaggc atgacaccta aaaggaagga aggccgaaag acgacggcag aagtggggat
     8941 ctctgaaacc agatcccttc tcctcttcac tccctcctcc tgatccctgg ccatcagtcc
     9001 tgctcctcgg ccacgaagtg cacgcagttg ccggccgggt cgcgcagggc gaactcccgc
     9061 ccccacggct gctcgccgat ctcggtcatg gccggcccgg aggcgtcccg gaagttcgtg
     9121 gacacgacct ccgaccactc ggcgtacagc tcgtccaggc cgcgcaccca cacccaggcc
     9181 agggtgttgt ccggcaccac ctggtcctgg accgcgctga tgaacagggt cacgtcgtcc
     9241 cggaccacac cggcgaagtc gtcctccacg aagtcccggg agaacccgag ccggtcggtc
     9301 cagaactcga ccgctccggc gacgtcgcgc gcggtgagca ccggaacggc actggtcaac
     9361 ttggccatct gctcggaggg gaggatgaag ggaaggaggg aggggggagg ggcatttatg
     9421 acaattagaa cttcagaggt ctactccttg tgtgtaacga ggtgaacgac gggtgaaagt
     9481 gttcgcctga caggactggt cgaagatcgg ggggaggggg ctacctctat ctctgagggc
     9541 atctttctga taaggtgcct tcaaccgcaa ccccgaaaac ccctgccccc caccacacca
     9601 tggacacatg cacgcacgac aaatagacag agcgatgcca acaggattgg ttgtatagta
     9661 tggttctggt aagatagagg gattaaatgc cctgctccag gccgtgaggg gcgaactggc
     9721 gcgcttgtgc ttctccttct gtacttaatc tacatccctg accttgaact ggatcttgac
     9781 aaatatagat ccgaaaaccc actgccgcac gaacttacca taatttaaaa agaggtttta
     9841 gaggcgaaga gtggagactt ctgtgtgagg tttgtgaaaa ggtaagaagg aggggacagg
     9901 agatcatgaa tctatatctt aattaagcac ttctgctgtt ttgaatgggg ttagagcaca
     9961 agtgggatgc ctggactttc agggcatgca agcacacact acgaccacaa cagaagtcaa
    10021 aagggtgtag atagagtttg gcctcttgtc tgatgggcat tcacccggca gtatcctagt
    10081 taaaacaaag acacatgaag agatataaag atcatcgcgt gtgccctcta gacgggcggg
    10141 ctatagagga aaggacaaaa gaccgccaaa ccgacgcagg atgcggacag gttcagtgca
    10201 ccaaggagga ggaaacgccc aaccacgagg tccaaggctg gcagagaaat aaacgacccc
    10261 gtttaaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc
    10321 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc
    10381 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc
    10441 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt
    10501 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg
    10561 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat
    10621 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag
    10681 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt
    10741 ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc
    10801 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta
    10861 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag
    10921 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga
    10981 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa
    11041 gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa
    11101 tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc
    11161 ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga
    11221 taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa
    11281 gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt
    11341 gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg
    11401 ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc
    11461 aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg
    11521 gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag
    11581 cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt
    11641 actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt
    11701 caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac
    11761 gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac
    11821 ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag
    11881 caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa
    11941 tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga
    12001 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc
    12061 cccgaaaagt gccacctggg tccttttc
//
