LOCUS       sequence-346571-       11576 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pNOC episomal plasmid harboring the humanized fnCas12a gene sequence
            without Nlux tag and crRNA targeting the Nitrate reductase gene of 
            N. oceanica IMET1 with spacer 1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11576)
  AUTHORS   Naduthodi MIS, Sudfeld C, Avitzigiannis EK, Trevisan N, van Lith E, 
            Alcaide Sancho J, D'Adamo S, Barbosa M, van der Oost J
  TITLE     Comprehensive Genome Engineering Toolbox for Microalgae 
            Nannochloropsis oceanica Based on CRISPR-Cas Systems.
  JOURNAL   ACS Synth Biol. 2021 Dec 17;10(12):3369-3378. doi: 
            10.1021/acssynbio.1c00329. Epub 2021 Nov 18.
  PUBMED    34793143
REFERENCE   2  (bases 1 to 11576)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.1c00329"; journalName: "ACS Synth Biol"; date: 
            "2021-12-17- 17"; volume: "10"; issue: "12"; pages: "3369-3378"
COMMENT     Sequence Label: pNOC_hfnCas12a_crRNA NR1
FEATURES             Location/Qualifiers
     source          1..11576
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(1..589)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(81..100)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(760..1551,1552..1620))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1383..1402
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1621..1725)
                     /gene="bla"
                     /label=AmpR promoter
     misc_feature    1762..2265
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
     ncRNA           complement(3294..3361)
                     /product="hepatitis delta virus self-cleaving ribozyme 
                     (Ferre-D'Amare and Scott, 2010)"
                     /label=HDV ribozyme
                     /ncRNA_class="ribozyme"
     ncRNA           complement(3423..3459)
                     /product="self-cleaving hammerhead ribozyme (Ferre-D'Amare 
                     and Scott, 2010)"
                     /label=hammerhead ribozyme
                     /ncRNA_class="hammerhead_ribozyme"
     CDS             4456..4467
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             4688..4708
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             8606..8632
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     regulatory      8634..8643
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             8648..8668
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     regulatory      9349..9358
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(10243..10617)
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
ORIGIN
        1 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
       61 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
      121 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
      181 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
      241 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
      301 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
      361 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
      421 taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac
      481 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
      541 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
      601 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
      661 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
      721 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga
      781 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt
      841 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg
      901 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga
      961 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga
     1021 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg
     1081 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc
     1141 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc
     1201 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca
     1261 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac
     1321 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg
     1381 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc
     1441 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg
     1501 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac
     1561 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat
     1621 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
     1681 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
     1741 agtgccacct gggtcctttt catcacgtgc tataaaaata attataattt aaatttttta
     1801 atataaatat ataaattaaa aatagaaagt aaaaaaagaa attaaagaaa aaatagtttt
     1861 tgttttccga agatgtaaaa gactctaggg ggatcgccaa caaatactac cttttatctt
     1921 gtacttcctg ctctcaggta ttaatgccga attgtttcat cttgtctgtg tagaagacca
     1981 cacacgaaaa tcctgtgatt ttacatttta cttatcgtta atcgaatgta tatctattta
     2041 atctgctttt cttgtctaat aaatatatat gtaaagtacg ctttttgttg aaatttttta
     2101 aacctttgtt tatttttttt tcttcattcc gtaactcttc taccttcttt atttactttc
     2161 taaaatccaa atacaaaaca taaaaataaa taaacacaga gtaaattccc aaattattcc
     2221 atcattaaaa gatacgaggc gcgtgtaagt tacaggcaag cgatccgtct aagaaaccgg
     2281 tgcaaagctg acgccctttt cgtcgtcgtt gctggtgtta aaattgcgcg tttctttttt
     2341 ttccgtcttt gtcgtctttt gactttttta tcttcttttc tgtcttcttt tattcttctc
     2401 ttcttcttca tcactccctc ttcatttttc ttcttctttt tcctcgtcta ttgtcctcgg
     2461 gcattaccag ttgtgctgct cacgtgcgcg caagcggtga aagggtgaga ggatacacgg
     2521 acaacaatca ttgaaaatta cgcttaaagc cggtgcctgg aaaagtccac gcgcagatgg
     2581 cctttgcttg ccttgctgct gctgcagctg ctgctgctgt cgcctgtgat ggtaatggtt
     2641 gttgttgtgg tcctgcttgc ttgctagttt gcattgcgtt tgcggttgcc ttatgaccga
     2701 ctccgtgtga ttgatcctta aacatatttt atcattgatg tggattgtgt gacgacggca
     2761 aaaaagaatt actttacaca tgactatcac acctgctccc ctcccttctc ctttcaatcg
     2821 gtcaacttcc caaccgctcc tcgaaaatat ttcagttgaa tcctctacta cgtacccctt
     2881 acttaaattt actttctctc tccccatcaa tgcgccgttg ttctgaagtt gtattattga
     2941 tgggagatga ttggcactgc accagacctg gccaaaagcc accacaggtg gccacatact
     3001 cttgcatggg tatactctac cgaggcaaag attaaagtgc acgtcccctg cccctctctc
     3061 tccctacttc cttcagtctc agtggttccg tgcccgttca gcactacatc gccctgcccg
     3121 ctgttccttc tcaaacagcc acccatccca acccacatac acagaaacaa cctttcctac
     3181 acattttccc aagtcttctc ttcactattc tacactaata caccaccgtg atatgccggt
     3241 tatctagatg tcctttctcc tttctcatcc tttctttccc atggtaccca ggagtcccat
     3301 tcgccatgcc gaagcatgtt gcccagccgg cgccagcgag gaggctggga ccatgccggc
     3361 ccaggacgcg attgtcgtgg aagtggatct acaacagtag aaattattta aagttcttag
     3421 acgacgagct tactcgtttc gtcctcacgg actcatcagg tctaagaaga gccaaggcct
     3481 gctcttcatc gattctgtgg atggagggag ggaaggaggg ggggagtgag tgagtcgaga
     3541 gaacgacgac tggggcaaag agaggatgac gccactgcaa caaaagacag ggaagaatag
     3601 aggctgttgg aaaacaggaa cacagtgaag aatagaggct gcagggtaac aggcacattt
     3661 atcgcagaga ggcgggcggc aggggcgata cgtaaagaga cccctaaact tctccgcgtc
     3721 atccgttcac gctacctcct tccctccctt tactcccatt gatcaccaag gcagcgcccg
     3781 cagccaccag cagccaccgt cctcagtgtc acagctagac gcgtccccat caggcagcag
     3841 accaccagaa tatgcgcttt cgcttcccat catatccctc cctcctcccc ttctattcct
     3901 catcatgcat catttctttc tccttgctgc atgcccctct tcttaccttg gctaagatct
     3961 accggaggaa gaagtcttca acgatagata aatgcctgta tcgtgatggt tgtttgaatg
     4021 gtggaagtga gccaggtatt gagaacgagg ggcgcaaggg tgcgtggaag aaggcaggga
     4081 gaggcgaggc gaaggagaag ggcatgtggt gggaacgaag cctccacacc acagccaccc
     4141 atgcttttct ctgttcgagt tcccgggttc tactctcaca cactacacgt gcacagaaaa
     4201 cattcacgca caacagagat acagagggat ccgtcgagat ggtctacacg tcctaatgtc
     4261 ccctgctttc gaagcccaga gccatcgctt tctgttttta tccattcgca acacatcctg
     4321 ttctcgcgac ctctcctctt cctggccacc atcgcttctc cgtgccatgc cctgtacatc
     4381 cttccctccc tcgttgtcta ccacactaat catgcgctct gcagcagaca ctcggctact
     4441 atttgtgtcg tacggattga ggggagggag gggaagggag gcaagaagta aatcggtggt
     4501 caacaacaga cgcggtctcc ccaccctccc tcattccttt cccctcctat aatgagcgaa
     4561 gaaagccatg gacaccgagt cacccactct cctaatccac caccaccagg gcactaacgg
     4621 actcacgctc accaaactcg gtaacaatat ccactgcaca cagatacaca cgcaaccctc
     4681 gagcatgccc aagaaaaagc ggaaggtgag catctaccag gagttcgtca acaagtattc
     4741 actgagtaag acactgcggt tcgagctgat cccacagggc aagacactgg agaacatcaa
     4801 ggcccgaggc ctgattctgg acgatgagaa gcgggcaaaa gactataaga aagccaagca
     4861 gatcattgat aaataccacc agttctttat cgaggaaatt ctgagctccg tgtgcatcag
     4921 tgaggatctg ctgcagaatt actcagacgt gtacttcaag ctgaagaaga gcgacgatga
     4981 caacctgcag aaggacttca agtccgccaa ggacaccatc aagaaacaga ttagcgagta
     5041 catcaaggac tccgaaaagt ttaaaaatct gttcaaccag aatctgatcg atgctaagaa
     5101 aggccaggag tccgacctga tcctgtggct gaaacagtct aaggacaatg ggattgaact
     5161 gttcaaggct aactccgata tcactgatat tgacgaggca ctggaaatca tcaagagctt
     5221 caagggatgg accacatact ttaaaggctt ccacgagaac cgcaagaacg tgtactccag
     5281 caacgacatt cctacctcca tcatctaccg aatcgtcgat gacaatctgc caaagttcct
     5341 ggagaacaag gccaaatatg aatctctgaa ggacaaagct cccgaggcaa ttaattacga
     5401 acagatcaag aaagatctgg ctgaggaact gacattcgat atcgactata agactagcga
     5461 ggtgaaccag agggtctttt ccctggacga ggtgtttgaa atcgccaatt tcaacaatta
     5521 cctgaaccag tccggcatta ctaaattcaa taccatcatt ggcgggaagt ttgtgaacgg
     5581 ggagaatacc aagcgcaagg gaattaacga atacatcaat ctgtatagcc agcagatcaa
     5641 cgacaaaact ctgaagaaat acaagatgtc tgtgctgttc aaacagatcc tgagtgatac
     5701 cgagtccaag tcttttgtca ttgataaact ggaagatgac tcagacgtgg tcactaccat
     5761 gcagagcttt tatgagcaga tcgccgcttt caagacagtg gaggaaaaat ctattaagga
     5821 aactctgagt ctgctgttcg atgacctgaa agcccagaag ctggacctga gtaagatcta
     5881 cttcaaaaac gataagagtc tgacagacct gtcacagcag gtgtttgatg actattccgt
     5941 gattgggacc gccgtcctgg agtacattac acagcagatc gctccaaaga acctggataa
     6001 tccctctaag aaagagcagg aactgatcgc taagaaaacc gagaaggcaa aatatctgag
     6061 tctggaaaca attaagctgg cactggagga gttcaacaag cacagggata ttgacaaaca
     6121 gtgccgcttt gaggaaatcc tggccaactt cgcagccatc cccatgattt ttgatgagat
     6181 cgcccagaac aaagacaatc tggctcagat cagtattaag taccagaacc agggcaagaa
     6241 agacctgctg caggcttcag cagaagatga cgtgaaagcc atcaaggatc tgctggacca
     6301 gaccaacaat ctgctgcaca agctgaaaat cttccatatt agtcagtcag aggataaggc
     6361 taatatcctg gataaagacg aacacttcta cctggtgttc gaggaatgtt acttcgagct
     6421 ggcaaacatt gtccccctgt ataacaagat taggaactac atcacacaga agccttactc
     6481 tgacgagaag tttaaactga acttcgaaaa tagtaccctg gccaacgggt gggataagaa
     6541 caaggagcct gacaacacag ctatcctgtt catcaaggat gacaagtact atctgggagt
     6601 gatgaataag aaaaacaata agatcttcga tgacaaagcc attaaggaga acaaagggga
     6661 aggatacaag aaaatcgtgt ataagctgct gcccggcgca aataagatgc tgcctaaggt
     6721 gttcttcagc gccaagagta tcaaattcta caacccatcc gaggacatcc tgcggattag
     6781 aaatcactca acacatacta agaacgggag cccccagaag ggatatgaga aatttgagtt
     6841 caacatcgag gattgcagga agtttattga cttctacaag cagagcatct ccaaacaccc
     6901 tgaatggaag gattttggct tccggttttc cgacacacag agatataact ctatcgacga
     6961 gttctaccgc gaggtggaaa atcaggggta taagctgact tttgagaaca tttctgaaag
     7021 ttacatcgac agcgtggtca atcagggaaa gctgtacctg ttccagatct ataacaaaga
     7081 tttttcagca tacagcaagg gcagaccaaa cctgcataca ctgtactgga aggccctgtt
     7141 cgatgagagg aatctgcagg acgtggtcta taaactgaac ggagaggccg aactgtttta
     7201 ccggaagcag tctattccta agaaaatcac tcacccagct aaggaggcca tcgctaacaa
     7261 gaacaaggac aatcctaaga aagagagcgt gttcgaatac gatctgatta aggacaagcg
     7321 gttcaccgaa gataagttct ttttccattg tccaatcacc attaacttca agtcaagcgg
     7381 cgctaacaag ttcaacgacg agatcaatct gctgctgaag gaaaaagcaa acgatgtgca
     7441 catcctgagc attgaccgag gagagcggca tctggcctac tataccctgg tggatggcaa
     7501 agggaatatc attaagcagg atacattcaa catcattggc aatgaccgga tgaaaaccaa
     7561 ctaccacgat aaactggctg caatcgagaa ggatagagac tcagctagga aggactggaa
     7621 gaaaatcaac aacattaagg agatgaagga aggctatctg agccaggtgg tccatgagat
     7681 tgcaaagctg gtcatcgaat acaatgccat tgtggtgttc gaggatctga acttcggctt
     7741 taagaggggg cgctttaagg tggaaaaaca ggtctatcag aagctggaga aaatgctgat
     7801 cgaaaagctg aattacctgg tgtttaaaga taacgagttc gacaagaccg gaggcgtcct
     7861 gagagcctac cagctgacag ctccctttga aactttcaag aaaatgggaa aacagacagg
     7921 catcatctac tatgtgccag ccggattcac ttccaagatc tgccccgtga ccggctttgt
     7981 caaccagctg taccctaaat atgagtcagt gagcaagtcc caggaatttt tcagcaagtt
     8041 cgataagatc tgttataatc tggacaaggg gtacttcgag ttttccttcg attacaagaa
     8101 cttcggcgac aaggccgcta aggggaaatg gaccattgcc tccttcggat ctcgcctgat
     8161 caactttcga aattccgata aaaaccacaa ttgggacact agggaggtgt acccaaccaa
     8221 ggagctggaa aagctgctga aagactactc tatcgagtat ggacatggcg aatgcatcaa
     8281 ggcagccatc tgtggcgaga gtgataagaa atttttcgcc aagctgacct cagtgctgaa
     8341 tacaatcctg cagatgcgga actcaaagac cgggacagaa ctggactatc tgattagccc
     8401 cgtggctgat gtcaacggaa acttcttcga cagcagacag gcacccaaaa atatgcctca
     8461 ggatgcagac gccaacgggg cctaccacat cgggctgaag ggactgatgc tgctgggccg
     8521 gatcaagaac aatcaggagg ggaagaagct gaacctggtc attaagaacg aggaatactt
     8581 cgagtttgtc cagaatagaa ataactaccc ctacgacgtg cccgactacg cgtccatggt
     8641 ggctagcccg aaaaagaaga ggaaggtctg acaattggaa agatccaaga gagacgagta
     8701 gagatttttt ttttgggatt gatgtttgtc gttctttgag ttgtcgtcga gttacgcctt
     8761 ttgtaagaat gttccgcagg agaggaggag gatgggcatg agtgagggtg agagggcttg
     8821 cccgcttttt tttttaaaaa cgctgaagac gtggttgtca aacaaacccc ccatagaaac
     8881 gattttgtta cggtgcggtc cagacgtcac ttgaatggct ccgcggaaag gccagggagg
     8941 gaagggggga gggaggaaac atgaaacatg ttgaacggct caacagggtt tgggggacaa
     9001 gagaggtagc gccctgatgg actgctccct cccctccttt ccctcaatgt ctcattcatc
     9061 catgcttccc ccttctctct ctcccctccg ttccatcccc cgcgggcgtg gtagtggcgt
     9121 gatgggatcc actaaaatgt acgtgtaaga aaagccggtg agcttacgct tttgtgaaag
     9181 tgggagtacg agtgttgtgt gtgtgtgtag tggtttcaga ccccagacag aggcgaagca
     9241 gaaaaagcag acgatgaaga cgacgaagaa atgagcagtc tatttttatc gtggaaacag
     9301 aagaggtgat atcgtctcct gcaggcatgc aagctgatcc actagaggcc atggcggccg
     9361 cgagggtgga cttgagagtg tggaagtgag tgagggtaga agatagagta aggatggcag
     9421 acaagatgac gataaagaag acgtggaaga gagggaaata cacgtacaaa ggaagaaaac
     9481 aagaaaagga aaaagaggaa aaaataaacg caggcggaat tactcactgt tgaaatggtc
     9541 tctcgtcctg acacggaagt tgtacgaaca acacgcatgc acacaacgta ctcttcttct
     9601 gtgttctaaa taaagactat ttgctaaggg agggtagtgg cgatggtggc gtacagtgac
     9661 ttaatgggga ggagcaaatc aatgatgcct tttttggcat ttttctattg tctatctgcg
     9721 gtactacaat tcgttgttat acagtcacgg atggaaggaa gaagcagcag tagagtaggg
     9781 gataaaccaa tgagataagg gagctgatgt aacgagacca ggacgcctga aattaaagat
     9841 agaaaaacca caagtagagc caggagcgat gaccatcttc catttcgttt cttcacaaca
     9901 gtgcttttgc tttcttcgct tctactctct ccttactggc taccatgaca ttgttcactt
     9961 ctttcatttc tccttccctc tctcacacac atcccacccc ttcgatttcc ttctgccctt
    10021 cttccctcgg ggtctagtct tattttgatt gaaccgatgc gtgaattgga tcgagtgact
    10081 tgtatgacgg gcacacacac acatgacgac gacaggtgag gacttgaagg acctaaggca
    10141 tgacacctaa aaggaaggaa ggccgaaaga cgacggcaga agtggggatc tctgaaacca
    10201 gatcccttct cctcttcact ccctcctcct gatccctggc catcagtcct gctcctcggc
    10261 cacgaagtgc acgcagttgc cggccgggtc gcgcagggcg aactcccgcc cccacggctg
    10321 ctcgccgatc tcggtcatgg ccggcccgga ggcgtcccgg aagttcgtgg acacgacctc
    10381 cgaccactcg gcgtacagct cgtccaggcc gcgcacccac acccaggcca gggtgttgtc
    10441 cggcaccacc tggtcctgga ccgcgctgat gaacagggtc acgtcgtccc ggaccacacc
    10501 ggcgaagtcg tcctccacga agtcccggga gaacccgagc cggtcggtcc agaactcgac
    10561 cgctccggcg acgtcgcgcg cggtgagcac cggaacggca ctggtcaact tggccatctg
    10621 ctcggagggg aggatgaagg gaaggaggga ggggggaggg gcatttatga caattagaac
    10681 ttcagaggtc tactccttgt gtgtaacgag gtgaacgacg ggtgaaagtg ttcgcctgac
    10741 aggactggtc gaagatcggg gggagggggc tacctctatc tctgagggca tctttctgat
    10801 aaggtgcctt caaccgcaac cccgaaaacc cctgcccccc accacaccat ggacacatgc
    10861 acgcacgaca aatagacaga gcgatgccaa caggattggt tgtatagtat ggttctggta
    10921 agatagaggg attaaatgcc ctgctccagg ccgtgagggg cgaactggcg cgcttgtgct
    10981 tctccttctg tacttaatct acatccctga ccttgaactg gatcttgaca aatatagatc
    11041 cgaaaaccca ctgccgcacg aacttaccat aatttaaaaa gaggttttag aggcgaagag
    11101 tggagacttc tgtgtgaggt ttgtgaaaag gtaagaagga ggggacagga gatcatgaat
    11161 ctatatctta attaagcact tctgctgttt tgaatggggt tagagcacaa gtgggatgcc
    11221 tggactttca gggcatgcaa gcacacacta cgaccacaac agaagtcaaa agggtgtaga
    11281 tagagtttgg cctcttgtct gatgggcatt cacccggcag tatcctagtt aaaacaaaga
    11341 cacatgaaga gatataaaga tcatcgcgtg tgccctctag acgggcgggc tatagaggaa
    11401 aggacaaaag accgccaaac cgacgcagga tgcggacagg ttcagtgcac caaggaggag
    11461 gaaacgccca accacgaggt ccaaggctgg cagagaaata aacgaccccg tttaaacatg
    11521 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtt
//
