LOCUS       sequence-346573-       12116 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pNOC episomal plasmid harboring the humanized fnCas12a gene sequence
            tagged with Nlux and crRNA targeting the Nitrate reductase gene of 
            N. oceanica IMET1 with spacer 1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12116)
  AUTHORS   Naduthodi MIS, Sudfeld C, Avitzigiannis EK, Trevisan N, van Lith E, 
            Alcaide Sancho J, D'Adamo S, Barbosa M, van der Oost J
  TITLE     Comprehensive Genome Engineering Toolbox for Microalgae 
            Nannochloropsis oceanica Based on CRISPR-Cas Systems.
  JOURNAL   ACS Synth Biol. 2021 Dec 17;10(12):3369-3378. doi: 
            10.1021/acssynbio.1c00329. Epub 2021 Nov 18.
  PUBMED    34793143
REFERENCE   2  (bases 1 to 12116)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.1c00329"; journalName: "ACS Synth Biol"; date: 
            "2021-12-17- 17"; volume: "10"; issue: "12"; pages: "3369-3378"
COMMENT     Sequence Label: pNOC_hfnCas12a-Nlux_crRNA NR1
FEATURES             Location/Qualifiers
     source          1..12116
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(215..319)
                     /gene="bla"
                     /label=AmpR promoter
     misc_feature    356..859
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
     ncRNA           complement(1888..1955)
                     /product="hepatitis delta virus self-cleaving ribozyme 
                     (Ferre-D'Amare and Scott, 2010)"
                     /label=HDV ribozyme
                     /ncRNA_class="ribozyme"
     ncRNA           complement(2017..2053)
                     /product="self-cleaving hammerhead ribozyme (Ferre-D'Amare 
                     and Scott, 2010)"
                     /label=hammerhead ribozyme
                     /ncRNA_class="hammerhead_ribozyme"
     CDS             3050..3061
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             3282..3302
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             7740..7766
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     regulatory      7768..7777
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             7782..7802
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     regulatory      8483..8492
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(9377..9751)
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     rep_origin      complement(10711..11299)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10791..10810)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(11470..12116,1..145,146..214))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     12093..12112
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac
       61 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt
      121 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg
      181 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa
      241 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata
      301 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgggtcc ttttcatcac
      361 gtgctataaa aataattata atttaaattt tttaatataa atatataaat taaaaataga
      421 aagtaaaaaa agaaattaaa gaaaaaatag tttttgtttt ccgaagatgt aaaagactct
      481 agggggatcg ccaacaaata ctacctttta tcttgtactt cctgctctca ggtattaatg
      541 ccgaattgtt tcatcttgtc tgtgtagaag accacacacg aaaatcctgt gattttacat
      601 tttacttatc gttaatcgaa tgtatatcta tttaatctgc ttttcttgtc taataaatat
      661 atatgtaaag tacgcttttt gttgaaattt tttaaacctt tgtttatttt tttttcttca
      721 ttccgtaact cttctacctt ctttatttac tttctaaaat ccaaatacaa aacataaaaa
      781 taaataaaca cagagtaaat tcccaaatta ttccatcatt aaaagatacg aggcgcgtgt
      841 aagttacagg caagcgatcc gtctaagaaa ccggtgcaaa gctgacgccc ttttcgtcgt
      901 cgttgctggt gttaaaattg cgcgtttctt ttttttccgt ctttgtcgtc ttttgacttt
      961 tttatcttct tttctgtctt cttttattct tctcttcttc ttcatcactc cctcttcatt
     1021 tttcttcttc tttttcctcg tctattgtcc tcgggcatta ccagttgtgc tgctcacgtg
     1081 cgcgcaagcg gtgaaagggt gagaggatac acggacaaca atcattgaaa attacgctta
     1141 aagccggtgc ctggaaaagt ccacgcgcag atggcctttg cttgccttgc tgctgctgca
     1201 gctgctgctg ctgtcgcctg tgatggtaat ggttgttgtt gtggtcctgc ttgcttgcta
     1261 gtttgcattg cgtttgcggt tgccttatga ccgactccgt gtgattgatc cttaaacata
     1321 ttttatcatt gatgtggatt gtgtgacgac ggcaaaaaag aattacttta cacatgacta
     1381 tcacacctgc tcccctccct tctcctttca atcggtcaac ttcccaaccg ctcctcgaaa
     1441 atatttcagt tgaatcctct actacgtacc ccttacttaa atttactttc tctctcccca
     1501 tcaatgcgcc gttgttctga agttgtatta ttgatgggag atgattggca ctgcaccaga
     1561 cctggccaaa agccaccaca ggtggccaca tactcttgca tgggtatact ctaccgaggc
     1621 aaagattaaa gtgcacgtcc cctgcccctc tctctcccta cttccttcag tctcagtggt
     1681 tccgtgcccg ttcagcacta catcgccctg cccgctgttc cttctcaaac agccacccat
     1741 cccaacccac atacacagaa acaacctttc ctacacattt tcccaagtct tctcttcact
     1801 attctacact aatacaccac cgtgatatgc cggttatcta gatgtccttt ctcctttctc
     1861 atcctttctt tcccatggta cccaggagtc ccattcgcca tgccgaagca tgttgcccag
     1921 ccggcgccag cgaggaggct gggaccatgc cggcccagga cgcgattgtc gtggaagtgg
     1981 atctacaaca gtagaaatta tttaaagttc ttagacgacg agcttactcg tttcgtcctc
     2041 acggactcat caggtctaag aagagccaag gcctgctctt catcgattct gtggatggag
     2101 ggagggaagg agggggggag tgagtgagtc gagagaacga cgactggggc aaagagagga
     2161 tgacgccact gcaacaaaag acagggaaga atagaggctg ttggaaaaca ggaacacagt
     2221 gaagaataga ggctgcaggg taacaggcac atttatcgca gagaggcggg cggcaggggc
     2281 gatacgtaaa gagaccccta aacttctccg cgtcatccgt tcacgctacc tccttccctc
     2341 cctttactcc cattgatcac caaggcagcg cccgcagcca ccagcagcca ccgtcctcag
     2401 tgtcacagct agacgcgtcc ccatcaggca gcagaccacc agaatatgcg ctttcgcttc
     2461 ccatcatatc cctccctcct ccccttctat tcctcatcat gcatcatttc tttctccttg
     2521 ctgcatgccc ctcttcttac cttggctaag atctaccgga ggaagaagtc ttcaacgata
     2581 gataaatgcc tgtatcgtga tggttgtttg aatggtggaa gtgagccagg tattgagaac
     2641 gaggggcgca agggtgcgtg gaagaaggca gggagaggcg aggcgaagga gaagggcatg
     2701 tggtgggaac gaagcctcca caccacagcc acccatgctt ttctctgttc gagttcccgg
     2761 gttctactct cacacactac acgtgcacag aaaacattca cgcacaacag agatacagag
     2821 ggatccgtcg agatggtcta cacgtcctaa tgtcccctgc tttcgaagcc cagagccatc
     2881 gctttctgtt tttatccatt cgcaacacat cctgttctcg cgacctctcc tcttcctggc
     2941 caccatcgct tctccgtgcc atgccctgta catccttccc tccctcgttg tctaccacac
     3001 taatcatgcg ctctgcagca gacactcggc tactatttgt gtcgtacgga ttgaggggag
     3061 ggaggggaag ggaggcaaga agtaaatcgg tggtcaacaa cagacgcggt ctccccaccc
     3121 tccctcattc ctttcccctc ctataatgag cgaagaaagc catggacacc gagtcaccca
     3181 ctctcctaat ccaccaccac cagggcacta acggactcac gctcaccaaa ctcggtaaca
     3241 atatccactg cacacagata cacacgcaac cctcgagcat gcccaagaaa aagcggaagg
     3301 tgagcatcta ccaggagttc gtcaacaagt attcactgag taagacactg cggttcgagc
     3361 tgatcccaca gggcaagaca ctggagaaca tcaaggcccg aggcctgatt ctggacgatg
     3421 agaagcgggc aaaagactat aagaaagcca agcagatcat tgataaatac caccagttct
     3481 ttatcgagga aattctgagc tccgtgtgca tcagtgagga tctgctgcag aattactcag
     3541 acgtgtactt caagctgaag aagagcgacg atgacaacct gcagaaggac ttcaagtccg
     3601 ccaaggacac catcaagaaa cagattagcg agtacatcaa ggactccgaa aagtttaaaa
     3661 atctgttcaa ccagaatctg atcgatgcta agaaaggcca ggagtccgac ctgatcctgt
     3721 ggctgaaaca gtctaaggac aatgggattg aactgttcaa ggctaactcc gatatcactg
     3781 atattgacga ggcactggaa atcatcaaga gcttcaaggg atggaccaca tactttaaag
     3841 gcttccacga gaaccgcaag aacgtgtact ccagcaacga cattcctacc tccatcatct
     3901 accgaatcgt cgatgacaat ctgccaaagt tcctggagaa caaggccaaa tatgaatctc
     3961 tgaaggacaa agctcccgag gcaattaatt acgaacagat caagaaagat ctggctgagg
     4021 aactgacatt cgatatcgac tataagacta gcgaggtgaa ccagagggtc ttttccctgg
     4081 acgaggtgtt tgaaatcgcc aatttcaaca attacctgaa ccagtccggc attactaaat
     4141 tcaataccat cattggcggg aagtttgtga acggggagaa taccaagcgc aagggaatta
     4201 acgaatacat caatctgtat agccagcaga tcaacgacaa aactctgaag aaatacaaga
     4261 tgtctgtgct gttcaaacag atcctgagtg ataccgagtc caagtctttt gtcattgata
     4321 aactggaaga tgactcagac gtggtcacta ccatgcagag cttttatgag cagatcgccg
     4381 ctttcaagac agtggaggaa aaatctatta aggaaactct gagtctgctg ttcgatgacc
     4441 tgaaagccca gaagctggac ctgagtaaga tctacttcaa aaacgataag agtctgacag
     4501 acctgtcaca gcaggtgttt gatgactatt ccgtgattgg gaccgccgtc ctggagtaca
     4561 ttacacagca gatcgctcca aagaacctgg ataatccctc taagaaagag caggaactga
     4621 tcgctaagaa aaccgagaag gcaaaatatc tgagtctgga aacaattaag ctggcactgg
     4681 aggagttcaa caagcacagg gatattgaca aacagtgccg ctttgaggaa atcctggcca
     4741 acttcgcagc catccccatg atttttgatg agatcgccca gaacaaagac aatctggctc
     4801 agatcagtat taagtaccag aaccagggca agaaagacct gctgcaggct tcagcagaag
     4861 atgacgtgaa agccatcaag gatctgctgg accagaccaa caatctgctg cacaagctga
     4921 aaatcttcca tattagtcag tcagaggata aggctaatat cctggataaa gacgaacact
     4981 tctacctggt gttcgaggaa tgttacttcg agctggcaaa cattgtcccc ctgtataaca
     5041 agattaggaa ctacatcaca cagaagcctt actctgacga gaagtttaaa ctgaacttcg
     5101 aaaatagtac cctggccaac gggtgggata agaacaagga gcctgacaac acagctatcc
     5161 tgttcatcaa ggatgacaag tactatctgg gagtgatgaa taagaaaaac aataagatct
     5221 tcgatgacaa agccattaag gagaacaaag gggaaggata caagaaaatc gtgtataagc
     5281 tgctgcccgg cgcaaataag atgctgccta aggtgttctt cagcgccaag agtatcaaat
     5341 tctacaaccc atccgaggac atcctgcgga ttagaaatca ctcaacacat actaagaacg
     5401 ggagccccca gaagggatat gagaaatttg agttcaacat cgaggattgc aggaagttta
     5461 ttgacttcta caagcagagc atctccaaac accctgaatg gaaggatttt ggcttccggt
     5521 tttccgacac acagagatat aactctatcg acgagttcta ccgcgaggtg gaaaatcagg
     5581 ggtataagct gacttttgag aacatttctg aaagttacat cgacagcgtg gtcaatcagg
     5641 gaaagctgta cctgttccag atctataaca aagatttttc agcatacagc aagggcagac
     5701 caaacctgca tacactgtac tggaaggccc tgttcgatga gaggaatctg caggacgtgg
     5761 tctataaact gaacggagag gccgaactgt tttaccggaa gcagtctatt cctaagaaaa
     5821 tcactcaccc agctaaggag gccatcgcta acaagaacaa ggacaatcct aagaaagaga
     5881 gcgtgttcga atacgatctg attaaggaca agcggttcac cgaagataag ttctttttcc
     5941 attgtccaat caccattaac ttcaagtcaa gcggcgctaa caagttcaac gacgagatca
     6001 atctgctgct gaaggaaaaa gcaaacgatg tgcacatcct gagcattgac cgaggagagc
     6061 ggcatctggc ctactatacc ctggtggatg gcaaagggaa tatcattaag caggatacat
     6121 tcaacatcat tggcaatgac cggatgaaaa ccaactacca cgataaactg gctgcaatcg
     6181 agaaggatag agactcagct aggaaggact ggaagaaaat caacaacatt aaggagatga
     6241 aggaaggcta tctgagccag gtggtccatg agattgcaaa gctggtcatc gaatacaatg
     6301 ccattgtggt gttcgaggat ctgaacttcg gctttaagag ggggcgcttt aaggtggaaa
     6361 aacaggtcta tcagaagctg gagaaaatgc tgatcgaaaa gctgaattac ctggtgttta
     6421 aagataacga gttcgacaag accggaggcg tcctgagagc ctaccagctg acagctccct
     6481 ttgaaacttt caagaaaatg ggaaaacaga caggcatcat ctactatgtg ccagccggat
     6541 tcacttccaa gatctgcccc gtgaccggct ttgtcaacca gctgtaccct aaatatgagt
     6601 cagtgagcaa gtcccaggaa tttttcagca agttcgataa gatctgttat aatctggaca
     6661 aggggtactt cgagttttcc ttcgattaca agaacttcgg cgacaaggcc gctaagggga
     6721 aatggaccat tgcctccttc ggatctcgcc tgatcaactt tcgaaattcc gataaaaacc
     6781 acaattggga cactagggag gtgtacccaa ccaaggagct ggaaaagctg ctgaaagact
     6841 actctatcga gtatggacat ggcgaatgca tcaaggcagc catctgtggc gagagtgata
     6901 agaaattttt cgccaagctg acctcagtgc tgaatacaat cctgcagatg cggaactcaa
     6961 agaccgggac agaactggac tatctgatta gccccgtggc tgatgtcaac ggaaacttct
     7021 tcgacagcag acaggcaccc aaaaatatgc ctcaggatgc agacgccaac ggggcctacc
     7081 acatcgggct gaagggactg atgctgctgg gccggatcaa gaacaatcag gaggggaaga
     7141 agctgaacct ggtcattaag aacgaggaat acttcgagtt tgtccagaat agaaataacg
     7201 ggtccggcag cgggtccgtt aacatggtgt ttactctcga ggacttcgtg ggcgactggc
     7261 ggcagacagc tggctataac ctcgaccagg tgctggagca gggcggtgtg tcctcgcttt
     7321 tccagaacct gggcgtctcc gtgaccccaa tccagcgcat cgtcctgagc ggcgagaacg
     7381 gcctgaagat cgacatccac gtcatcattc cctacgaggg cctgagcggg gaccaaatgg
     7441 gccagatcga gaagatcttc aaggttgtgt accccgtgga cgatcaccac ttcaaggtga
     7501 tactgcacta cgggacgctg gtcatcgacg gtgtgacgcc gaacatgatt gactacttcg
     7561 gccgtcctta cgagggcatt gccgtgttcg acggcaagaa gatcacggtg accggcaccc
     7621 tgtggaacgg caataagatc attgacgagc ggctgatcaa cccggatggc tcgctgttgt
     7681 tccgcgtgac catcaacggg gtaaccggct ggcgcctgtg cgagcgcatc ctggccggat
     7741 acccctacga cgtgcccgac tacgcgtcca tggtggctag cccgaaaaag aagaggaagg
     7801 tctgacaatt ggaaagatcc aagagagacg agtagagatt ttttttttgg gattgatgtt
     7861 tgtcgttctt tgagttgtcg tcgagttacg ccttttgtaa gaatgttccg caggagagga
     7921 ggaggatggg catgagtgag ggtgagaggg cttgcccgct ttttttttta aaaacgctga
     7981 agacgtggtt gtcaaacaaa ccccccatag aaacgatttt gttacggtgc ggtccagacg
     8041 tcacttgaat ggctccgcgg aaaggccagg gagggaaggg gggagggagg aaacatgaaa
     8101 catgttgaac ggctcaacag ggtttggggg acaagagagg tagcgccctg atggactgct
     8161 ccctcccctc ctttccctca atgtctcatt catccatgct tcccccttct ctctctcccc
     8221 tccgttccat cccccgcggg cgtggtagtg gcgtgatggg atccactaaa atgtacgtgt
     8281 aagaaaagcc ggtgagctta cgcttttgtg aaagtgggag tacgagtgtt gtgtgtgtgt
     8341 gtagtggttt cagaccccag acagaggcga agcagaaaaa gcagacgatg aagacgacga
     8401 agaaatgagc agtctatttt tatcgtggaa acagaagagg tgatatcgtc tcctgcaggc
     8461 atgcaagctg atccactaga ggccatggcg gccgcgaggg tggacttgag agtgtggaag
     8521 tgagtgaggg tagaagatag agtaaggatg gcagacaaga tgacgataaa gaagacgtgg
     8581 aagagaggga aatacacgta caaaggaaga aaacaagaaa aggaaaaaga ggaaaaaata
     8641 aacgcaggcg gaattactca ctgttgaaat ggtctctcgt cctgacacgg aagttgtacg
     8701 aacaacacgc atgcacacaa cgtactcttc ttctgtgttc taaataaaga ctatttgcta
     8761 agggagggta gtggcgatgg tggcgtacag tgacttaatg gggaggagca aatcaatgat
     8821 gccttttttg gcatttttct attgtctatc tgcggtacta caattcgttg ttatacagtc
     8881 acggatggaa ggaagaagca gcagtagagt aggggataaa ccaatgagat aagggagctg
     8941 atgtaacgag accaggacgc ctgaaattaa agatagaaaa accacaagta gagccaggag
     9001 cgatgaccat cttccatttc gtttcttcac aacagtgctt ttgctttctt cgcttctact
     9061 ctctccttac tggctaccat gacattgttc acttctttca tttctccttc cctctctcac
     9121 acacatccca ccccttcgat ttccttctgc ccttcttccc tcggggtcta gtcttatttt
     9181 gattgaaccg atgcgtgaat tggatcgagt gacttgtatg acgggcacac acacacatga
     9241 cgacgacagg tgaggacttg aaggacctaa ggcatgacac ctaaaaggaa ggaaggccga
     9301 aagacgacgg cagaagtggg gatctctgaa accagatccc ttctcctctt cactccctcc
     9361 tcctgatccc tggccatcag tcctgctcct cggccacgaa gtgcacgcag ttgccggccg
     9421 ggtcgcgcag ggcgaactcc cgcccccacg gctgctcgcc gatctcggtc atggccggcc
     9481 cggaggcgtc ccggaagttc gtggacacga cctccgacca ctcggcgtac agctcgtcca
     9541 ggccgcgcac ccacacccag gccagggtgt tgtccggcac cacctggtcc tggaccgcgc
     9601 tgatgaacag ggtcacgtcg tcccggacca caccggcgaa gtcgtcctcc acgaagtccc
     9661 gggagaaccc gagccggtcg gtccagaact cgaccgctcc ggcgacgtcg cgcgcggtga
     9721 gcaccggaac ggcactggtc aacttggcca tctgctcgga ggggaggatg aagggaagga
     9781 gggagggggg aggggcattt atgacaatta gaacttcaga ggtctactcc ttgtgtgtaa
     9841 cgaggtgaac gacgggtgaa agtgttcgcc tgacaggact ggtcgaagat cggggggagg
     9901 gggctacctc tatctctgag ggcatctttc tgataaggtg ccttcaaccg caaccccgaa
     9961 aacccctgcc ccccaccaca ccatggacac atgcacgcac gacaaataga cagagcgatg
    10021 ccaacaggat tggttgtata gtatggttct ggtaagatag agggattaaa tgccctgctc
    10081 caggccgtga ggggcgaact ggcgcgcttg tgcttctcct tctgtactta atctacatcc
    10141 ctgaccttga actggatctt gacaaatata gatccgaaaa cccactgccg cacgaactta
    10201 ccataattta aaaagaggtt ttagaggcga agagtggaga cttctgtgtg aggtttgtga
    10261 aaaggtaaga aggaggggac aggagatcat gaatctatat cttaattaag cacttctgct
    10321 gttttgaatg gggttagagc acaagtggga tgcctggact ttcagggcat gcaagcacac
    10381 actacgacca caacagaagt caaaagggtg tagatagagt ttggcctctt gtctgatggg
    10441 cattcacccg gcagtatcct agttaaaaca aagacacatg aagagatata aagatcatcg
    10501 cgtgtgccct ctagacgggc gggctataga ggaaaggaca aaagaccgcc aaaccgacgc
    10561 aggatgcgga caggttcagt gcaccaagga ggaggaaacg cccaaccacg aggtccaagg
    10621 ctggcagaga aataaacgac cccgtttaaa catgtgagca aaaggccagc aaaaggccag
    10681 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
    10741 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
    10801 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
    10861 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
    10921 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
    10981 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
    11041 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
    11101 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt
    11161 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
    11221 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
    11281 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
    11341 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
    11401 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg
    11461 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg
    11521 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc
    11581 atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc
    11641 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc
    11701 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag
    11761 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat
    11821 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg
    11881 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt
    11941 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag
    12001 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg
    12061 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaa
//
