LOCUS       sequence-346577-       11374 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pNOC episomal plasmid harboring the dead version of humanized 
            fnCas12a gene sequence tagged with Nlux and spacer sequence is 
            replaced by the type IIS restriction site for endonuclease BaeI that
            can be.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11374)
  AUTHORS   Naduthodi MIS, Sudfeld C, Avitzigiannis EK, Trevisan N, van Lith E, 
            Alcaide Sancho J, D'Adamo S, Barbosa M, van der Oost J
  TITLE     Comprehensive Genome Engineering Toolbox for Microalgae 
            Nannochloropsis oceanica Based on CRISPR-Cas Systems.
  JOURNAL   ACS Synth Biol. 2021 Dec 17;10(12):3369-3378. doi: 
            10.1021/acssynbio.1c00329. Epub 2021 Nov 18.
  PUBMED    34793143
REFERENCE   2  (bases 1 to 11374)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.1c00329"; journalName: "ACS Synth Biol"; date: 
            "2021-12-17- 17"; volume: "10"; issue: "12"; pages: "3369-3378"
COMMENT     Sequence Label: pNOC_dfnCas12a_crRNA NC
FEATURES             Location/Qualifiers
     source          1..11374
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     ncRNA           complement(1138..1205)
                     /product="hepatitis delta virus self-cleaving ribozyme 
                     (Ferre-D'Amare and Scott, 2010)"
                     /label=HDV ribozyme
                     /ncRNA_class="ribozyme"
     ncRNA           complement(1253..1289)
                     /product="self-cleaving hammerhead ribozyme (Ferre-D'Amare 
                     and Scott, 2010)"
                     /label=hammerhead ribozyme
                     /ncRNA_class="hammerhead_ribozyme"
     CDS             2271..2282
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             2503..2523
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             6961..6987
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     regulatory      6989..6998
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             7003..7023
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     regulatory      7705..7714
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     rep_origin      complement(9219..9807)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9299..9318)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9978..10769,10770..10838))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10601..10620
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10839..10943)
                     /gene="bla"
                     /label=AmpR promoter
     misc_feature    join(10980..11374,1..109)
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
ORIGIN
        1 tttctaaaat ccaaatacaa aacataaaaa taaataaaca cagagtaaat tcccaaatta
       61 ttccatcatt aaaagatacg aggcgcgtgt aagttacagg caagcgatcc gtctaagaaa
      121 ccggtgcaaa gctgacgccc ttttcgtcgt cgttgctggt gttaaaattg cgcgtttctt
      181 ttttttccgt ctttgtcgtc ttttgacttt tttatcttct tttctgtctt cttttattct
      241 tctcttcttc ttcatcactc cctcttcatt tttcttcttc tttttcctcg tctattgtcc
      301 tcgggcatta ccagttgtgc tgctcacgtg cgcgcaagcg gtgaaagggt gagaggatac
      361 acggacaaca atcattgaaa attacgctta aagccggtgc ctggaaaagt ccacgcgcag
      421 atggcctttg cttgccttgc tgctgctgca gctgctgctg ctgtcgcctg tgatggtaat
      481 ggttgttgtt gtggtcctgc ttgcttgcta gtttgcattg cgtttgcggt tgccttatga
      541 ccgactccgt gtgattgatc cttaaacata ttttatcatt gatgtggatt gtgtgacgac
      601 ggcaaaaaag aattacttta cacatgacta tcacacctgc tcccctccct tctcctttca
      661 atcggtcaac ttcccaaccg ctcctcgaaa atatttcagt tgaatcctct actacgtacc
      721 ccttacttaa atttactttc tctctcccca tcaatgcgcc gttgttctga agttgtatta
      781 ttgatgggag atgattggca ctgcaccaga cctggccaaa agccaccaca ggtggccaca
      841 tactcttgca tgggtatact ctaccgaggc aaagattaaa gtgcacgtcc cctgcccctc
      901 tctctcccta cttccttcag tctcagtggt tccgtgcccg ttcagcacta catcgccctg
      961 cccgctgttc cttctcaaac agccacccat cccaacccac atacacagaa acaacctttc
     1021 ctacacattt tcccaagtct tctcttcact attctacact aatacaccac cgtgatatgc
     1081 cggttatcta gatgtccttt ctcctttctc atcctttctt tcccatggta cccaggagtc
     1141 ccattcgcca tgccgaagca tgttgcccag ccggcgccag cgaggaggct gggaccatgc
     1201 cggccccagc tggatactag cgtcatggga tccatctaca acagtagaaa ttgacgagct
     1261 tactcgtttc gtcctcacgg actcatcaga atttcgctct tcatcgattc tgtggatgga
     1321 gggagggaag gaggggggga gtgagtgagt cgagagaacg acgactgggg caaagagagg
     1381 atgacgccac tgcaacaaaa gacagggaag aatagaggct gttggaaaac aggaacacag
     1441 tgaagaatag aggctgcagg gtaacaggca catttatcgc agagaggcgg gcggcagggg
     1501 cgatacgtaa agagacccct aaacttctcc gcgtcatccg ttcacgctac ctccttccct
     1561 ccctttactc ccattgatca ccaaggcagc gcccgcagcc accatcagcc accgtcctca
     1621 gtgtcacagc tagacgcgtc cccatcaggc agcagaccac cagaatatgc gctttcgctt
     1681 cccatcatat ccctccctcc tccccttcta ttcctcatca tgcatcattt ctttctcctt
     1741 gctgcatgcc cctcttctta ccttggctaa gatctaccgg aggaagaagt cttcaacgat
     1801 agataaatgc ctgtatcgtg atggttgttt gaatggtgga agtgagccag gtattgagaa
     1861 cgaggggcgc aagggtgcgt ggaagaaggc agggagaggc gaggcgaagg agaagggcat
     1921 gtggtgggaa cgaagcctcc acaccacagc cacccatgct tttctctgtt cgagttcccg
     1981 ggttctactc tcacacacta cacgtgcaca gaaaacattc acgcacaaca gagatacaga
     2041 gggatccgtc gagatggtct acacgtccta atgtcccctg ctttcgaagc ccagagccat
     2101 cgctttctgt ttttatccat tcgcaacaca tcctgttctc gcgacctctc ctcttcctgg
     2161 ccaccatcgc ttctccgtgc catgccctgt acatccttcc ctccctcgtt gtctaccaca
     2221 ctaatcatgc gctctgcagc agacactcgg ctactatttg tgtcgtacgg attgagggga
     2281 gggaggggaa gggaggcaag aagtaaatcg gtggtcaaca acagacgcgg tctccccacc
     2341 ctccctcatt cctttcccct cctataatga gcgaagaaag ccatggacac cgagtcaccc
     2401 actctcctaa tccaccacca ccagggcact aacggactca cgctcaccaa actcggtaac
     2461 aatatccact gcacacagat acacacgcaa ccctcgagca tgcccaagaa aaagcggaag
     2521 gtgagcatct accaggagtt cgtcaacaag tattcactga gtaagacact gcggttcgag
     2581 ctgatcccac agggcaagac actggagaac atcaaggccc gaggcctgat tctggacgat
     2641 gagaagcggg caaaagacta taagaaagcc aagcagatca ttgataaata ccaccagttc
     2701 tttatcgagg aaattctgag ctccgtgtgc atcagtgagg atctgctgca gaattactca
     2761 gacgtgtact tcaagctgaa gaagagcgac gatgacaacc tgcagaagga cttcaagtcc
     2821 gccaaggaca ccatcaagaa acagattagc gagtacatca aggactccga aaagtttaaa
     2881 aatctgttca accagaatct gatcgatgct aagaaaggcc aggagtccga cctgatcctg
     2941 tggctgaaac agtctaagga caatgggatt gaactgttca aggctaactc cgatatcact
     3001 gatattgacg aggcactgga aatcatcaag agcttcaagg gatggaccac atactttaaa
     3061 ggcttccacg agaaccgcaa gaacgtgtac tccagcaacg acattcctac ctccatcatc
     3121 taccgaatcg tcgatgacaa tctgccaaag ttcctggaga acaaggccaa atatgaatct
     3181 ctgaaggaca aagctcccga ggcaattaat tacgaacaga tcaagaaaga tctggctgag
     3241 gaactgacat tcgatatcga ctataagact agcgaggtga accagagggt cttttccctg
     3301 gacgaggtgt ttgaaatcgc caatttcaac aattacctga accagtccgg cattactaaa
     3361 ttcaatacca tcattggcgg gaagtttgtg aacggggaga ataccaagcg caagggaatt
     3421 aacgaataca tcaatctgta tagccagcag atcaacgaca aaactctgaa gaaatacaag
     3481 atgtctgtgc tgttcaaaca gatcctgagt gataccgagt ccaagtcttt tgtcattgat
     3541 aaactggaag atgactcaga cgtggtcact accatgcaga gcttttatga gcagatcgcc
     3601 gctttcaaga cagtggagga aaaatctatt aaggaaactc tgagtctgct gttcgatgac
     3661 ctgaaagccc agaagctgga cctgagtaag atctacttca aaaacgataa gagtctgaca
     3721 gacctgtcac agcaggtgtt tgatgactat tccgtgattg ggaccgccgt cctggagtac
     3781 attacacagc agatcgctcc aaagaacctg gataatccct ctaagaaaga gcaggaactg
     3841 atcgctaaga aaaccgagaa ggcaaaatat ctgagtctgg aaacaattaa gctggcactg
     3901 gaggagttca acaagcacag ggatattgac aaacagtgcc gctttgagga aatcctggcc
     3961 aacttcgcag ccatccccat gatttttgat gagatcgccc agaacaaaga caatctggct
     4021 cagatcagta ttaagtacca gaaccagggc aagaaagacc tgctgcaggc ttcagcagaa
     4081 gatgacgtga aagccatcaa ggatctgctg gaccagacca acaatctgct gcacaagctg
     4141 aaaatcttcc atattagtca gtcagaggat aaggctaata tcctggataa agacgaacac
     4201 ttctacctgg tgttcgagga atgttacttc gagctggcaa acattgtccc cctgtataac
     4261 aagattagga actacatcac acagaagcct tactctgacg agaagtttaa actgaacttc
     4321 gaaaatagta ccctggccaa cgggtgggat aagaacaagg agcctgacaa cacagctatc
     4381 ctgttcatca aggatgacaa gtactatctg ggagtgatga ataagaaaaa caataagatc
     4441 ttcgatgaca aagccattaa ggagaacaaa ggggaaggat acaagaaaat cgtgtataag
     4501 ctgctgcccg gcgcaaataa gatgctgcct aaggtgttct tcagcgccaa gagtatcaaa
     4561 ttctacaacc catccgagga catcctgcgg attagaaatc actcaacaca tactaagaac
     4621 gggagccccc agaagggata tgagaaattt gagttcaaca tcgaggattg caggaagttt
     4681 attgacttct acaagcagag catctccaaa caccctgaat ggaaggattt tggcttccgg
     4741 ttttccgaca cacagagata taactctatc gacgagttct accgcgaggt ggaaaatcag
     4801 gggtataagc tgacttttga gaacatttct gaaagttaca tcgacagcgt ggtcaatcag
     4861 ggaaagctgt acctgttcca gatctataac aaagattttt cagcatacag caagggcaga
     4921 ccaaacctgc atacactgta ctggaaggcc ctgttcgatg agaggaatct gcaggacgtg
     4981 gtctataaac tgaacggaga ggccgaactg ttttaccgga agcagtctat tcctaagaaa
     5041 atcactcacc cagctaagga ggccatcgct aacaagaaca aggacaatcc taagaaagag
     5101 agcgtgttcg aatacgatct gattaaggac aagcggttca ccgaagataa gttctttttc
     5161 cattgtccaa tcaccattaa cttcaagtca agcggcgcta acaagttcaa cgacgagatc
     5221 aatctgctgc tgaaggaaaa agcaaacgat gtgcacatcc tgagcattgc ccgaggagag
     5281 cggcatctgg cctactatac cctggtggat ggcaaaggga atatcattaa gcaggataca
     5341 ttcaacatca ttggcaatga ccggatgaaa accaactacc acgataaact ggctgcaatc
     5401 gagaaggata gagactcagc taggaaggac tggaagaaaa tcaacaacat taaggagatg
     5461 aaggaaggct atctgagcca ggtggtccat gagattgcaa agctggtcat cgaatacaat
     5521 gccattgtgg tgttcgcgga tctgaacttc ggctttaaga gggggcgctt taaggtggaa
     5581 aaacaggtct atcagaagct ggagaaaatg ctgatcgaaa agctgaatta cctggtgttt
     5641 aaagataacg agttcgacaa gaccggaggc gtcctgagag cctaccagct gacagctccc
     5701 tttgaaactt tcaagaaaat gggaaaacag acaggcatca tctactatgt gccagccgga
     5761 ttcacttcca agatctgccc cgtgaccggc tttgtcaacc agctgtaccc taaatatgag
     5821 tcagtgagca agtcccagga atttttcagc aagttcgata agatctgtta taatctggac
     5881 aaggggtact tcgagttttc cttcgattac aagaacttcg gcgacaaggc cgctaagggg
     5941 aaatggacca ttgcctcctt cggatctcgc ctgatcaact ttcgaaattc cgataaaaac
     6001 cacaattggg acactaggga ggtgtaccca accaaggagc tggaaaagct gctgaaagac
     6061 tactctatcg agtatggaca tggcgaatgc atcaaggcag ccatctgtgg cgagagtgat
     6121 aagaaatttt tcgccaagct gacctcagtg ctgaatacaa tcctgcagat gcggaactca
     6181 aagaccggga cagaactgga ctatctgatt agccccgtgg ctgatgtcaa cggaaacttc
     6241 ttcgacagca gacaggcacc caaaaatatg cctcaggatg cagacgccaa cggggcctac
     6301 cacatcgggc tgaagggact gatgctgctg ggccggatca agaacaatca ggaggggaag
     6361 aagctgaacc tggtcattaa gaacgaggaa tacttcgagt ttgtccagaa tagaaataac
     6421 gggtccggca gcgggtccgt taacatggtg tttactctcg aggacttcgt gggcgactgg
     6481 cggcagacag ctggctataa cctcgaccag gtgctggagc agggcggtgt gtcctcgctt
     6541 ttccagaacc tgggcgtctc cgtgacccca atccagcgca tcgtcctgag cggcgagaac
     6601 ggcctgaaga tcgacatcca cgtcatcatt ccctacgagg gcctgagcgg ggaccaaatg
     6661 ggccagatcg agaagatctt caaggttgtg taccccgtgg acgatcacca cttcaaggtg
     6721 atactgcact acgggacgct ggtcatcgac ggtgtgacgc cgaacatgat tgactacttc
     6781 ggccgtcctt acgagggcat tgccgtgttc gacggcaaga agatcacggt gaccggcacc
     6841 ctgtggaacg gcaataagat cattgacgag cggctgatca acccggatgg ctcgctgttg
     6901 ttccgcgtga ccatcaacgg ggtaaccggc tggcgcctgt gcgagcgcat cctggccgga
     6961 tacccctacg acgtgcccga ctacgcgtcc atggtggcta gcccgaaaaa gaagaggaag
     7021 gtctgacaat tggaaagatc caagagagac gagtagagat tttttttttg ggattgatgt
     7081 ttgtcgttct ttgagttgtc gtcgagttac gccttttgta agaatgttcc gcaggagagg
     7141 aggaggatgg gcatgagtga gggtgagagg gcttgcccgc tttttttttt taaaaacgct
     7201 gaagacgtgg ttgtcaaaca aaccccccat agaaacgatt ttgttacggt gcggtccaga
     7261 cgtcacttga atggctccgc ggaaaggcca gggagggaag gggggaggga ggaaacatga
     7321 aacatgttga acggctcaac agggtttggg ggacaagaga ggtagcgccc tgatggactg
     7381 ctccctcccc tcctttccct caatgtctca ttcatccatg cttccccctt ctctctctcc
     7441 cctccgttcc atcccccgcg ggcgtggtag tggcgtgatg ggatccacta aaatgtacgt
     7501 gtaagaaaag ccggtgagct tacgcttttg tgaaagtggg agtacgagtg ttgtgtgtgt
     7561 gtgtagtggt ttcagacccc agacagaggc gaagcagaaa aagcagacga tgaagacgac
     7621 gaagaaatga gcagtctatt tttatcgtgg aaacagaaga ggtgatatcg tctcctgcag
     7681 gcatgcaagc tgatccacta gaggccatgg cggccgcgat ggagtggatg gaggaggagg
     7741 cgagcgtagc agcaagcgtg agttatacag ccaggcacat gtcgcaatcc ttcggtctcg
     7801 ggcttaaaat ccacgcacta atcacgctgg gccatgcaaa gagcaatgcc gaggcccacc
     7861 acacaaaacg ctgtgtcgcg cgttgcggcc tgaagcttca tacttcttag tcgccgccaa
     7921 aagggctcga gagacgagac ccgttggcat gaccgatgtt gttcgacgcg gtttgcttcg
     7981 tcacagtcga cgtgattcag gaatctggag cctgcagatc atttttttca gcctgatatc
     8041 gttcttttcc actgagaacc atcagaccac cttttcttcc attgtgtgaa ggagtaggag
     8101 ttgccgtgct gctttgtggg agacatctgc gatggtgacc agcctcccgt cgtctggtcg
     8161 acgtgacgag cctcttcact gttcttcgac ggagagacgc aagcgagacg gctctagacc
     8221 ttttggacac gcattctgtg tgtgaactag tggacagtga taccacgtct gaaagctcac
     8281 cactgcccat ggtgcagcta cttgtcacaa agttttgact ccgtcggtat caccattcgc
     8341 gctcgtgtgc ctggttgttc cgccacgccg gcctgccccg gggcggggca atattctaaa
     8401 atctcacgca aaacaccgca cttacccctc acacatattc gtgatagacc accaccaatc
     8461 tcagcccgca tcaacacagg agggcccatg cccctctcgc aggaggagag cacgctgatc
     8521 gagagggcta cggcgacgat taactcgatt cctatctccg aggattactc ggtggcttcc
     8581 gctgctctgt cgtccgacgg acggatcttc acgggggtca acgtgtacca ttttacgggg
     8641 gggccttgcg cggagctggt ggtcctgggg accgccgctg ctgccgcggc tgggaacctg
     8701 acgtgcattg tcgccatcgg gaacgagaac cgcgggattc tgagcccctg cgggcggtgc
     8761 cggcaggtcc tgctcgacct ccaccctggg atcaaggcga ttgtgaagga ttccgacggg
     8821 cagcctacgg cggtcggtat cagagaactt ctcccttcgg gctacgtgtg ggagagcgct
     8881 tgaactagta gtagatgccg accggagtcc gcaaaaatca ccagtctctc tctacaaatc
     8941 tatctctctc tatttttctc cagaataatg tgtgagtagt tcccagataa gggaattagg
     9001 gttcttatag ggtttcgctc atgtgttgag catataagaa acccttagta tgtatttgta
     9061 tttgtaaaat acttctatca ataaaatttc taattcctaa aaccaaaatc cagtgacctg
     9121 gtttaaacca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa
     9181 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct
     9241 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
     9301 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
     9361 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca
     9421 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
     9481 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
     9541 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
     9601 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga
     9661 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc
     9721 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
     9781 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac
     9841 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc
     9901 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag
     9961 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt
    10021 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag
    10081 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca
    10141 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact
    10201 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca
    10261 gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg
    10321 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc
    10381 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg
    10441 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca
    10501 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt
    10561 atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc
    10621 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc
    10681 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca
    10741 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa
    10801 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat
    10861 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa
    10921 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctgg gtccttttca
    10981 tcacgtgcta taaaaataat tataatttaa attttttaat ataaatatat aaattaaaaa
    11041 tagaaagtaa aaaaagaaat taaagaaaaa atagtttttg ttttccgaag atgtaaaaga
    11101 ctctaggggg atcgccaaca aatactacct tttatcttgt acttcctgct ctcaggtatt
    11161 aatgccgaat tgtttcatct tgtctgtgta gaagaccaca cacgaaaatc ctgtgatttt
    11221 acattttact tatcgttaat cgaatgtata tctatttaat ctgcttttct tgtctaataa
    11281 atatatatgt aaagtacgct ttttgttgaa attttttaaa cctttgttta tttttttttc
    11341 ttcattccgt aactcttcta ccttctttat ttac
//
