LOCUS       sequence-346579-       12073 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pNOC episomal plasmid harboring the humanized fnCas12a gene sequence
            tagged with Nlux and crRNA targeting the Nitrate reductase gene of 
            N. oceanica IMET1 with spacer 1 without the hammerhead ribozym.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12073)
  AUTHORS   Naduthodi MIS, Sudfeld C, Avitzigiannis EK, Trevisan N, van Lith E, 
            Alcaide Sancho J, D'Adamo S, Barbosa M, van der Oost J
  TITLE     Comprehensive Genome Engineering Toolbox for Microalgae 
            Nannochloropsis oceanica Based on CRISPR-Cas Systems.
  JOURNAL   ACS Synth Biol. 2021 Dec 17;10(12):3369-3378. doi: 
            10.1021/acssynbio.1c00329. Epub 2021 Nov 18.
  PUBMED    34793143
REFERENCE   2  (bases 1 to 12073)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.1c00329"; journalName: "ACS Synth Biol"; date: 
            "2021-12-17- 17"; volume: "10"; issue: "12"; pages: "3369-3378"
COMMENT     Sequence Label: pNOC_hfnCas12a_Nlux_(-HH)crRNA NR1
FEATURES             Location/Qualifiers
     source          1..12073
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..504
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
     ncRNA           complement(1533..1600)
                     /product="hepatitis delta virus self-cleaving ribozyme 
                     (Ferre-D'Amare and Scott, 2010)"
                     /label=HDV ribozyme
                     /ncRNA_class="ribozyme"
     CDS             2652..2663
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             2884..2904
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             7342..7368
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     regulatory      7370..7379
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             7384..7404
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     regulatory      8085..8094
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(8979..9353)
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     rep_origin      complement(10313..10901)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10393..10412)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(11072..11863,11864..11932))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11695..11714
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11933..12037)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 atcacgtgct ataaaaataa ttataattta aattttttaa tataaatata taaattaaaa
       61 atagaaagta aaaaaagaaa ttaaagaaaa aatagttttt gttttccgaa gatgtaaaag
      121 actctagggg gatcgccaac aaatactacc ttttatcttg tacttcctgc tctcaggtat
      181 taatgccgaa ttgtttcatc ttgtctgtgt agaagaccac acacgaaaat cctgtgattt
      241 tacattttac ttatcgttaa tcgaatgtat atctatttaa tctgcttttc ttgtctaata
      301 aatatatatg taaagtacgc tttttgttga aattttttaa acctttgttt attttttttt
      361 cttcattccg taactcttct accttcttta tttactttct aaaatccaaa tacaaaacat
      421 aaaaataaat aaacacagag taaattccca aattattcca tcattaaaag atacgaggcg
      481 cgtgtaagtt acaggcaagc gatccgtcta agaaaccggt gcaaagctga cgcccttttc
      541 gtcgtcgttg ctggtgttaa aattgcgcgt ttcttttttt tccgtctttg tcgtcttttg
      601 acttttttat cttcttttct gtcttctttt attcttctct tcttcttcat cactccctct
      661 tcatttttct tcttcttttt cctcgtctat tgtcctcggg cattaccagt tgtgctgctc
      721 acgtgcgcgc aagcggtgaa agggtgagag gatacacgga caacaatcat tgaaaattac
      781 gcttaaagcc ggtgcctgga aaagtccacg cgcagatggc ctttgcttgc cttgctgctg
      841 ctgcagctgc tgctgctgtc gcctgtgatg gtaatggttg ttgttgtggt cctgcttgct
      901 tgctagtttg cattgcgttt gcggttgcct tatgaccgac tccgtgtgat tgatccttaa
      961 acatatttta tcattgatgt ggattgtgtg acgacggcaa aaaagaatta ctttacacat
     1021 gactatcaca cctgctcccc tcccttctcc tttcaatcgg tcaacttccc aaccgctcct
     1081 cgaaaatatt tcagttgaat cctctactac gtacccctta cttaaattta ctttctctct
     1141 ccccatcaat gcgccgttgt tctgaagttg tattattgat gggagatgat tggcactgca
     1201 ccagacctgg ccaaaagcca ccacaggtgg ccacatactc ttgcatgggt atactctacc
     1261 gaggcaaaga ttaaagtgca cgtcccctgc ccctctctct ccctacttcc ttcagtctca
     1321 gtggttccgt gcccgttcag cactacatcg ccctgcccgc tgttccttct caaacagcca
     1381 cccatcccaa cccacataca cagaaacaac ctttcctaca cattttccca agtcttctct
     1441 tcactattct acactaatac accaccgtga tatgccggtt atctagatgt cctttctcct
     1501 ttctcatcct ttctttccca tggtacccag gagtcccatt cgccatgccg aagcatgttg
     1561 cccagccggc gccagcgagg aggctgggac catgccggcc caggacgcga ttgtcgtgga
     1621 agtggatcta caacagtaga aattatttaa agttcttaga cgaagagcca aggcctgctc
     1681 ttcatcgatt ctgtggatgg agggagggaa ggaggggggg agtgagtgag tcgagagaac
     1741 gacgactggg gcaaagagag gatgacgcca ctgcaacaaa agacagggaa gaatagaggc
     1801 tgttggaaaa caggaacaca gtgaagaata gaggctgcag ggtaacaggc acatttatcg
     1861 cagagaggcg ggcggcaggg gcgatacgta aagagacccc taaacttctc cgcgtcatcc
     1921 gttcacgcta cctccttccc tccctttact cccattgatc accaaggcag cgcccgcagc
     1981 caccagcagc caccgtcctc agtgtcacag ctagacgcgt ccccatcagg cagcagacca
     2041 ccagaatatg cgctttcgct tcccatcata tccctccctc ctccccttct attcctcatc
     2101 atgcatcatt tctttctcct tgctgcatgc ccctcttctt accttggcta agatctaccg
     2161 gaggaagaag tcttcaacga tagataaatg cctgtatcgt gatggttgtt tgaatggtgg
     2221 aagtgagcca ggtattgaga acgaggggcg caagggtgcg tggaagaagg cagggagagg
     2281 cgaggcgaag gagaagggca tgtggtggga acgaagcctc cacaccacag ccacccatgc
     2341 ttttctctgt tcgagttccc gggttctact ctcacacact acacgtgcac agaaaacatt
     2401 cacgcacaac agagatacag agggatccgt cgagatggtc tacacgtcct aatgtcccct
     2461 gctttcgaag cccagagcca tcgctttctg tttttatcca ttcgcaacac atcctgttct
     2521 cgcgacctct cctcttcctg gccaccatcg cttctccgtg ccatgccctg tacatccttc
     2581 cctccctcgt tgtctaccac actaatcatg cgctctgcag cagacactcg gctactattt
     2641 gtgtcgtacg gattgagggg agggagggga agggaggcaa gaagtaaatc ggtggtcaac
     2701 aacagacgcg gtctccccac cctccctcat tcctttcccc tcctataatg agcgaagaaa
     2761 gccatggaca ccgagtcacc cactctccta atccaccacc accagggcac taacggactc
     2821 acgctcacca aactcggtaa caatatccac tgcacacaga tacacacgca accctcgagc
     2881 atgcccaaga aaaagcggaa ggtgagcatc taccaggagt tcgtcaacaa gtattcactg
     2941 agtaagacac tgcggttcga gctgatccca cagggcaaga cactggagaa catcaaggcc
     3001 cgaggcctga ttctggacga tgagaagcgg gcaaaagact ataagaaagc caagcagatc
     3061 attgataaat accaccagtt ctttatcgag gaaattctga gctccgtgtg catcagtgag
     3121 gatctgctgc agaattactc agacgtgtac ttcaagctga agaagagcga cgatgacaac
     3181 ctgcagaagg acttcaagtc cgccaaggac accatcaaga aacagattag cgagtacatc
     3241 aaggactccg aaaagtttaa aaatctgttc aaccagaatc tgatcgatgc taagaaaggc
     3301 caggagtccg acctgatcct gtggctgaaa cagtctaagg acaatgggat tgaactgttc
     3361 aaggctaact ccgatatcac tgatattgac gaggcactgg aaatcatcaa gagcttcaag
     3421 ggatggacca catactttaa aggcttccac gagaaccgca agaacgtgta ctccagcaac
     3481 gacattccta cctccatcat ctaccgaatc gtcgatgaca atctgccaaa gttcctggag
     3541 aacaaggcca aatatgaatc tctgaaggac aaagctcccg aggcaattaa ttacgaacag
     3601 atcaagaaag atctggctga ggaactgaca ttcgatatcg actataagac tagcgaggtg
     3661 aaccagaggg tcttttccct ggacgaggtg tttgaaatcg ccaatttcaa caattacctg
     3721 aaccagtccg gcattactaa attcaatacc atcattggcg ggaagtttgt gaacggggag
     3781 aataccaagc gcaagggaat taacgaatac atcaatctgt atagccagca gatcaacgac
     3841 aaaactctga agaaatacaa gatgtctgtg ctgttcaaac agatcctgag tgataccgag
     3901 tccaagtctt ttgtcattga taaactggaa gatgactcag acgtggtcac taccatgcag
     3961 agcttttatg agcagatcgc cgctttcaag acagtggagg aaaaatctat taaggaaact
     4021 ctgagtctgc tgttcgatga cctgaaagcc cagaagctgg acctgagtaa gatctacttc
     4081 aaaaacgata agagtctgac agacctgtca cagcaggtgt ttgatgacta ttccgtgatt
     4141 gggaccgccg tcctggagta cattacacag cagatcgctc caaagaacct ggataatccc
     4201 tctaagaaag agcaggaact gatcgctaag aaaaccgaga aggcaaaata tctgagtctg
     4261 gaaacaatta agctggcact ggaggagttc aacaagcaca gggatattga caaacagtgc
     4321 cgctttgagg aaatcctggc caacttcgca gccatcccca tgatttttga tgagatcgcc
     4381 cagaacaaag acaatctggc tcagatcagt attaagtacc agaaccaggg caagaaagac
     4441 ctgctgcagg cttcagcaga agatgacgtg aaagccatca aggatctgct ggaccagacc
     4501 aacaatctgc tgcacaagct gaaaatcttc catattagtc agtcagagga taaggctaat
     4561 atcctggata aagacgaaca cttctacctg gtgttcgagg aatgttactt cgagctggca
     4621 aacattgtcc ccctgtataa caagattagg aactacatca cacagaagcc ttactctgac
     4681 gagaagttta aactgaactt cgaaaatagt accctggcca acgggtggga taagaacaag
     4741 gagcctgaca acacagctat cctgttcatc aaggatgaca agtactatct gggagtgatg
     4801 aataagaaaa acaataagat cttcgatgac aaagccatta aggagaacaa aggggaagga
     4861 tacaagaaaa tcgtgtataa gctgctgccc ggcgcaaata agatgctgcc taaggtgttc
     4921 ttcagcgcca agagtatcaa attctacaac ccatccgagg acatcctgcg gattagaaat
     4981 cactcaacac atactaagaa cgggagcccc cagaagggat atgagaaatt tgagttcaac
     5041 atcgaggatt gcaggaagtt tattgacttc tacaagcaga gcatctccaa acaccctgaa
     5101 tggaaggatt ttggcttccg gttttccgac acacagagat ataactctat cgacgagttc
     5161 taccgcgagg tggaaaatca ggggtataag ctgacttttg agaacatttc tgaaagttac
     5221 atcgacagcg tggtcaatca gggaaagctg tacctgttcc agatctataa caaagatttt
     5281 tcagcataca gcaagggcag accaaacctg catacactgt actggaaggc cctgttcgat
     5341 gagaggaatc tgcaggacgt ggtctataaa ctgaacggag aggccgaact gttttaccgg
     5401 aagcagtcta ttcctaagaa aatcactcac ccagctaagg aggccatcgc taacaagaac
     5461 aaggacaatc ctaagaaaga gagcgtgttc gaatacgatc tgattaagga caagcggttc
     5521 accgaagata agttcttttt ccattgtcca atcaccatta acttcaagtc aagcggcgct
     5581 aacaagttca acgacgagat caatctgctg ctgaaggaaa aagcaaacga tgtgcacatc
     5641 ctgagcattg accgaggaga gcggcatctg gcctactata ccctggtgga tggcaaaggg
     5701 aatatcatta agcaggatac attcaacatc attggcaatg accggatgaa aaccaactac
     5761 cacgataaac tggctgcaat cgagaaggat agagactcag ctaggaagga ctggaagaaa
     5821 atcaacaaca ttaaggagat gaaggaaggc tatctgagcc aggtggtcca tgagattgca
     5881 aagctggtca tcgaatacaa tgccattgtg gtgttcgagg atctgaactt cggctttaag
     5941 agggggcgct ttaaggtgga aaaacaggtc tatcagaagc tggagaaaat gctgatcgaa
     6001 aagctgaatt acctggtgtt taaagataac gagttcgaca agaccggagg cgtcctgaga
     6061 gcctaccagc tgacagctcc ctttgaaact ttcaagaaaa tgggaaaaca gacaggcatc
     6121 atctactatg tgccagccgg attcacttcc aagatctgcc ccgtgaccgg ctttgtcaac
     6181 cagctgtacc ctaaatatga gtcagtgagc aagtcccagg aatttttcag caagttcgat
     6241 aagatctgtt ataatctgga caaggggtac ttcgagtttt ccttcgatta caagaacttc
     6301 ggcgacaagg ccgctaaggg gaaatggacc attgcctcct tcggatctcg cctgatcaac
     6361 tttcgaaatt ccgataaaaa ccacaattgg gacactaggg aggtgtaccc aaccaaggag
     6421 ctggaaaagc tgctgaaaga ctactctatc gagtatggac atggcgaatg catcaaggca
     6481 gccatctgtg gcgagagtga taagaaattt ttcgccaagc tgacctcagt gctgaataca
     6541 atcctgcaga tgcggaactc aaagaccggg acagaactgg actatctgat tagccccgtg
     6601 gctgatgtca acggaaactt cttcgacagc agacaggcac ccaaaaatat gcctcaggat
     6661 gcagacgcca acggggccta ccacatcggg ctgaagggac tgatgctgct gggccggatc
     6721 aagaacaatc aggaggggaa gaagctgaac ctggtcatta agaacgagga atacttcgag
     6781 tttgtccaga atagaaataa cgggtccggc agcgggtccg ttaacatggt gtttactctc
     6841 gaggacttcg tgggcgactg gcggcagaca gctggctata acctcgacca ggtgctggag
     6901 cagggcggtg tgtcctcgct tttccagaac ctgggcgtct ccgtgacccc aatccagcgc
     6961 atcgtcctga gcggcgagaa cggcctgaag atcgacatcc acgtcatcat tccctacgag
     7021 ggcctgagcg gggaccaaat gggccagatc gagaagatct tcaaggttgt gtaccccgtg
     7081 gacgatcacc acttcaaggt gatactgcac tacgggacgc tggtcatcga cggtgtgacg
     7141 ccgaacatga ttgactactt cggccgtcct tacgagggca ttgccgtgtt cgacggcaag
     7201 aagatcacgg tgaccggcac cctgtggaac ggcaataaga tcattgacga gcggctgatc
     7261 aacccggatg gctcgctgtt gttccgcgtg accatcaacg gggtaaccgg ctggcgcctg
     7321 tgcgagcgca tcctggccgg atacccctac gacgtgcccg actacgcgtc catggtggct
     7381 agcccgaaaa agaagaggaa ggtctgacaa ttggaaagat ccaagagaga cgagtagaga
     7441 tttttttttt gggattgatg tttgtcgttc tttgagttgt cgtcgagtta cgccttttgt
     7501 aagaatgttc cgcaggagag gaggaggatg ggcatgagtg agggtgagag ggcttgcccg
     7561 cttttttttt taaaaacgct gaagacgtgg ttgtcaaaca aaccccccat agaaacgatt
     7621 ttgttacggt gcggtccaga cgtcacttga atggctccgc ggaaaggcca gggagggaag
     7681 gggggaggga ggaaacatga aacatgttga acggctcaac agggtttggg ggacaagaga
     7741 ggtagcgccc tgatggactg ctccctcccc tcctttccct caatgtctca ttcatccatg
     7801 cttccccctt ctctctctcc cctccgttcc atcccccgcg ggcgtggtag tggcgtgatg
     7861 ggatccacta aaatgtacgt gtaagaaaag ccggtgagct tacgcttttg tgaaagtggg
     7921 agtacgagtg ttgtgtgtgt gtgtagtggt ttcagacccc agacagaggc gaagcagaaa
     7981 aagcagacga tgaagacgac gaagaaatga gcagtctatt tttatcgtgg aaacagaaga
     8041 ggtgatatcg tctcctgcag gcatgcaagc tgatccacta gaggccatgg cggccgcgag
     8101 ggtggacttg agagtgtgga agtgagtgag ggtagaagat agagtaagga tggcagacaa
     8161 gatgacgata aagaagacgt ggaagagagg gaaatacacg tacaaaggaa gaaaacaaga
     8221 aaaggaaaaa gaggaaaaaa taaacgcagg cggaattact cactgttgaa atggtctctc
     8281 gtcctgacac ggaagttgta cgaacaacac gcatgcacac aacgtactct tcttctgtgt
     8341 tctaaataaa gactatttgc taagggaggg tagtggcgat ggtggcgtac agtgacttaa
     8401 tggggaggag caaatcaatg atgccttttt tggcattttt ctattgtcta tctgcggtac
     8461 tacaattcgt tgttatacag tcacggatgg aaggaagaag cagcagtaga gtaggggata
     8521 aaccaatgag ataagggagc tgatgtaacg agaccaggac gcctgaaatt aaagatagaa
     8581 aaaccacaag tagagccagg agcgatgacc atcttccatt tcgtttcttc acaacagtgc
     8641 ttttgctttc ttcgcttcta ctctctcctt actggctacc atgacattgt tcacttcttt
     8701 catttctcct tccctctctc acacacatcc caccccttcg atttccttct gcccttcttc
     8761 cctcggggtc tagtcttatt ttgattgaac cgatgcgtga attggatcga gtgacttgta
     8821 tgacgggcac acacacacat gacgacgaca ggtgaggact tgaaggacct aaggcatgac
     8881 acctaaaagg aaggaaggcc gaaagacgac ggcagaagtg gggatctctg aaaccagatc
     8941 ccttctcctc ttcactccct cctcctgatc cctggccatc agtcctgctc ctcggccacg
     9001 aagtgcacgc agttgccggc cgggtcgcgc agggcgaact cccgccccca cggctgctcg
     9061 ccgatctcgg tcatggccgg cccggaggcg tcccggaagt tcgtggacac gacctccgac
     9121 cactcggcgt acagctcgtc caggccgcgc acccacaccc aggccagggt gttgtccggc
     9181 accacctggt cctggaccgc gctgatgaac agggtcacgt cgtcccggac cacaccggcg
     9241 aagtcgtcct ccacgaagtc ccgggagaac ccgagccggt cggtccagaa ctcgaccgct
     9301 ccggcgacgt cgcgcgcggt gagcaccgga acggcactgg tcaacttggc catctgctcg
     9361 gaggggagga tgaagggaag gagggagggg ggaggggcat ttatgacaat tagaacttca
     9421 gaggtctact ccttgtgtgt aacgaggtga acgacgggtg aaagtgttcg cctgacagga
     9481 ctggtcgaag atcgggggga gggggctacc tctatctctg agggcatctt tctgataagg
     9541 tgccttcaac cgcaaccccg aaaacccctg ccccccacca caccatggac acatgcacgc
     9601 acgacaaata gacagagcga tgccaacagg attggttgta tagtatggtt ctggtaagat
     9661 agagggatta aatgccctgc tccaggccgt gaggggcgaa ctggcgcgct tgtgcttctc
     9721 cttctgtact taatctacat ccctgacctt gaactggatc ttgacaaata tagatccgaa
     9781 aacccactgc cgcacgaact taccataatt taaaaagagg ttttagaggc gaagagtgga
     9841 gacttctgtg tgaggtttgt gaaaaggtaa gaaggagggg acaggagatc atgaatctat
     9901 atcttaatta agcacttctg ctgttttgaa tggggttaga gcacaagtgg gatgcctgga
     9961 ctttcagggc atgcaagcac acactacgac cacaacagaa gtcaaaaggg tgtagataga
    10021 gtttggcctc ttgtctgatg ggcattcacc cggcagtatc ctagttaaaa caaagacaca
    10081 tgaagagata taaagatcat cgcgtgtgcc ctctagacgg gcgggctata gaggaaagga
    10141 caaaagaccg ccaaaccgac gcaggatgcg gacaggttca gtgcaccaag gaggaggaaa
    10201 cgcccaacca cgaggtccaa ggctggcaga gaaataaacg accccgttta aacatgtgag
    10261 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata
    10321 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
    10381 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
    10441 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
    10501 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
    10561 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
    10621 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga
    10681 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg
    10741 gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa
    10801 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg
    10861 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt
    10921 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat
    10981 tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct
    11041 aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta
    11101 tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa
    11161 ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac
    11221 gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa
    11281 gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag
    11341 taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg
    11401 tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag
    11461 ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg
    11521 tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc
    11581 ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat
    11641 tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata
    11701 ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa
    11761 aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca
    11821 actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc
    11881 aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc
    11941 tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg
    12001 aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac
    12061 ctgggtcctt ttc
//
