LOCUS       sequence-346582-        9252 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector expressing N-terminally tagged APEX2-FLAG-LGALS3.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9252)
  AUTHORS   Eapen VV, Swarup S, Hoyer MJ, Paulo JA, Harper JW
  TITLE     Quantitative proteomics reveals the selectivity of ubiquitin-binding
            autophagy receptors in the turnover of damaged lysosomes by 
            lysophagy.
  JOURNAL   eLife. 2021 Sep 29;10. pii: 72328. doi: 10.7554/eLife.72328.
  PUBMED    34585663
REFERENCE   2  (bases 1 to 9252)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "eLife."; 
            date: "2021-09-29"; volume: "10. pii: 72328. doi"; pages: " 
            10.7554/eLife.72328"
COMMENT     Sequence Label: pHAGE-FLAG-APEX2-LGALS3
FEATURES             Location/Qualifiers
     source          1..9252
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(22..39)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(193..781)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(273..292)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(952..1743,1744..1812))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1575..1594
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1813..1917)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     1985..2003
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     LTR             2122..2355
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     misc_feature    2430..3018
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             2536..2547
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     2945..2965
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3035..3634)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(3118..3138)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     3615..3634
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     misc_feature    3646..4198
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    3648..4215
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(3808..3827)
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     primer_bind     4037..4054
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     protein_bind    4228..4252
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(5003..5027)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(5043..5789)
                     /codon_start=1
                     /product="soybean ascorbate peroxidase, engineered to serve
                     as a monomeric tag for electron microscopy and live-cell 
                     proteomics (Lam et al., 2015)"
                     /label=APEX2
                     /note="more active than the original APEX due to the A134P 
                     mutation"
                     /translation="GKSYPTVSADYQDAVEKAKKKLRGFIAEKRCAPLMLRLAFHSAGT
                     FDKGTKTGGPFGTIKHPAELAHSANNGLDIAVRLLEPLKAEFPILSYADFYQLAGVVAV
                     EVTGGPKVPFHPGREDKPEPPPEGRLPDPTKGSDHLRDVFGKAMGLTDQDIVALSGGHT
                     IGAAHKERSGFEGPWTSNPLIFDNSYFTELLSGEKEGLLQLPSDKALLSDPVFRPLVDK
                     YAADEDAFFADYAEAHQKLSELGFADA"
     CDS             complement(5790..5813)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     primer_bind     complement(5901..5925)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        complement(5922..6125)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(5951..5971)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        6126..6429
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     misc_feature    6481..6598
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(6895..6939)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    7124..7357
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    7854..7979
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             8026..8659
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(8851..8870)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8920..8936)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8920..8936)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8933..8955)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8944..8960
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8968..8974,8975..8992,8993..8998))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9013..9034
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg
       61 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa
      121 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
      181 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
      241 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
      301 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
      361 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta
      421 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
      481 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
      541 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
      601 gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct
      661 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
      721 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
      781 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
      841 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa
      901 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg
      961 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg
     1021 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc
     1081 aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc
     1141 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa
     1201 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc
     1261 cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg
     1321 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc
     1381 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat
     1441 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg
     1501 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc
     1561 ggcgtcaaca cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg
     1621 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat
     1681 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg
     1741 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg
     1801 ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct
     1861 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac
     1921 atttccccga aaagtgccac ctgacgtcta agaaaccatt attatcatga cattaaccta
     1981 taaaaatagg cgtatcacga ggccctttcg tcttcaagaa tgatctagcc ctttccttaa
     2041 ttaacccggg cctctcactc tctgatattc atttctttgc aagttataaa tactgaataa
     2101 taagatgaca tgaactacta ctgctagaga ttttccacac tgactaaaag ggtctgaggg
     2161 atctctagtt accagagtca cacaacagac gggcacacac tacttgaagc actcaaggca
     2221 agctttattg aggcttaagc agtgggttcc ctagttagcc agagagctcc caggctcaga
     2281 tctggtctaa ccagagagac ccagtacaag caaaaagcag atcttgtctt cgttgggagt
     2341 gaattagccc ttccagtccc cccttttctt ttaaaaagtg gctaagatct acagctgcct
     2401 tgtaagtcat tggtcttaaa ggatctcagg cggggaggcg gcccaaaggg agatccgact
     2461 cgtctgaggg cgaaggcgaa gacgcggaag aggccgcaga gccggcagca ggccgcggga
     2521 aggaaggtcc gctggattga gggccgaagg gacgtagcag aaggacgtcc cgcgcagaat
     2581 ccaggtggca acacaggcga gcagccaagg aaaggacgat gatttccccg acaacaccac
     2641 ggaattgtca gtgcccaaca gccgagcccc tgtccagcag cgggcaaggc aggcggcgat
     2701 gagttccgcc gtggcaatag ggagggggaa agcgaaagtc ccggaaagga gctgacaggt
     2761 ggtggcaatg ccccaaccag tgggggttgc gtcagcaaac acagtgcaca ccacgccacg
     2821 ttgcctgaca acgggccaca actcctcata aagagacagc aaccaggatt tatacaagga
     2881 ggagaaaatg aaagccatac gggaagcaat agcatgatac aaaggcatta aagcagcgta
     2941 tccacatagc gtaaaaggag caacatagtt aagaatacca gtcaatcttt cacaaatttt
     3001 gtaatccaga ggttgattag gatccatcga tgcatcaggc accgggcttg cgggtcatgc
     3061 accaggtgcg cggtccttcg ggcacctcga cgtcggcggt gacggtgaag ccgagccgct
     3121 cgtagaaggg gaggttgcgg ggcgcggagg tctccaggaa ggcgggcacc ccggcgcgct
     3181 cggccgcctc cactccgggg agcacgacgg cgctgcccag acccttgccc tggtggtcgg
     3241 gcgagacgcc gacggtggcc aggaaccacg cgggctcctt gggccggtgc ggcgccagga
     3301 ggccttccat ctgttgctgc gcggccagcc gggaaccgct caactcggcc atgcgcgggc
     3361 cgatctcggc gaacaccgcc cccgcttcga cgctctccgg cgtggtccag accgccaccg
     3421 cggcgccgtc gtccgcgacc cacaccttgc cgatgtcgag cccgacgcgc gtgaggaaga
     3481 gttcttgcag ctcggtgacc cgctcgatgt ggcggtccgg atcgacggtg tggcgcgtgg
     3541 cggggtagtc ggcgaacgcg gcggcgaggg tgcgtacggc cctggggacg tcgtcgcggg
     3601 tggcgaggcg caccgtgggc ttgtactcgg tcatggttgt ggccatatta tcatcgtgtt
     3661 tttcaaagga aaaccacgtc cccgtggttc ggggggccta gacgtttttt taacctcgac
     3721 taaacacatg taaagcatgt gcaccgaggc cccagatcag atcccataca atggggtacc
     3781 ttctgggcat ccttcagccc cttgttgaat acgcttgagg agagccattt gactctttcc
     3841 acaactatcc aactcacaac gtggcactgg ggttgtgccg cctttgcagg tgtatcttat
     3901 acacgtggct tttggccgca gaggcacctg tcgccaggtg gggggttccg ctgcctgcaa
     3961 agggtcgcta cagacgttgt ttgtcttcaa gaagcttcca gaggaactgc ttccttcacg
     4021 acattcaaca gaccttgcat tcctttggcg agaggggaaa gacccctagg aatgctcgtc
     4081 aagaagacag ggccaggttt ccgggccctc acattgccaa aagacggcaa tatggtggaa
     4141 aataacatat agacaaacgc acaccggcct tattccaagc ggcttcggcc agtaacgtta
     4201 gggggggggg gcggaattcg ttatcgaacc actttgtaca agaaagttgg gtatatcatg
     4261 gtatatgaag cactggtgag gtctatgtca ccagaaattc ccagtttgct gatttcattg
     4321 agttttttaa cccgatgatt gtactgcaac aagtgagcat cattcactgc aaccttgaag
     4381 tggtcaggtt caaccagtac ttgtattttg aatggtttcc cactttcaaa tgggaaaacc
     4441 gactgtcttt cttcccttcc ccagttatta tccagctttg tattgcaaac aatgactctc
     4501 ctgttgttct cattgaagcg tgggttaaag tggaaggcaa catcattccc tctttggaaa
     4561 tctaaagcaa ttctgtttgc attgggcttc accgtgccca gaattgttat cagcatgcga
     4621 ggcaccactc ccccaggcaa aggcaggtta taaggcacaa tcagtggccc agcaggggcg
     4681 ccataggggc cagtggcagg gtaggctccg ggggcacttg gctgtccaga agatgggtag
     4741 gccccagggc cgctgggtgg ccctgggtag actccaggtg caggtgctcc gggataagct
     4801 ccaggtgctc catggtaggc gcctggaggt gcctgtccag gataagcccc tgggggtgcc
     4861 tgcccggggt aggccccagg ataggaagcc cctgggtagc cccctgcccc agcaggctgg
     4921 ttcccccatg cgccaggcca tccttgaggg tttgggtttc cagacccaga taacgcatca
     4981 tggagcgaaa aattgtctgc catgccaact tttttgtaca aacttgtttg ataacctcga
     5041 gcggcatcag caaacccaag ctcggaaagc ttttggtgag cctcagcgta atcagcaaag
     5101 aaggcatctt cgtccgctgc atatttgtca acgagagggc ggaatacagg gtcagacaaa
     5161 agagccttgt cagaaggtag ctgaaggaga ccttccttct caccactcaa caactccgtg
     5221 aagtatgagt tgtcgaaaat aagaggatta gaggtccagg gaccctcaaa tccagaacgc
     5281 tccttgtgtg cagctccaat agtgtgaccc ccagatagag caacgatatc ttggtcagta
     5341 agccccatag ctttgccaaa cacatctctc aaatggtcag aacccttagt gggatcgggc
     5401 aagcgaccct ctggtggtgg ctcaggcttg tcctctcttc cagggtggaa tggaacctta
     5461 ggtccacccg tgacctcaac ggcaacaacg ccagccaact ggtagaaatc ggcgtagctc
     5521 aaaataggga actccgcctt gagtggctcc aaaagcctaa cagcgatgtc aagaccgttg
     5581 ttagcgctgt gagccagttc ggcagggtgc ttgatggttc cgaagggtcc accggtcttc
     5641 gtgcccttgt caaaggttcc agcagagtgg aatgccaaac ggagcattag aggagcgcat
     5701 ctcttctcag cgatgaagcc tctgagcttc ttcttcgcct tctcaacggc gtcctggtaa
     5761 tcagcactca cagttgggta agactttccc ttatcgtcgt catccttgta gtccatcatg
     5821 gtggcagatc aacctcgaga gatctgcggc cgccggtgtc ttctatggag gtcaaaacag
     5881 cgtggatggc gtctccaggc gatctgacgg ttcactaaac gagctctgct tatatagacc
     5941 tcccaccgta cacgcctacc gcccatttgc gtcaatgggg cggagttgtt acgacatttt
     6001 ggaaagtccc gttgattttg gtgccaaaac aaactcccat tgacgtcaat ggggtggaga
     6061 cttggaaatc cccgtgagtc aaaccgctat ccacgcccat tgatgtactg ccaaaaccgc
     6121 atcaccatgg taatagcgat gactaatacg tagatgtact gccaagtagg aaagtcccat
     6181 aaggtcatgt actgggcata atgccaggcg ggccatttac cgtcattgac gtcaataggg
     6241 ggcgtacttg gcatatgata cacttgatgt actgccaagt gggcagttta ccgtaaatac
     6301 tccacccatt gacgtcaatg gaaagtccct attggcgtta ctatgggaac atacgtcatt
     6361 attgacgtca atgggcgggg gtcgttgggc ggtcagccag gcgggccatt taccgtaagt
     6421 tatgtaacgc ggaactccac tagtccaaac tggatctctg ctgtccctgt aataaacccg
     6481 aaaattttga atttttgtaa tttgtttttg taattcttta gtttgtatgt ctgttgctat
     6541 tatgtctact attctttccc ctgcactgta ccccccaatc cccccttttc ttttaaaatt
     6601 gtggatgaat actgccattt gtgaattaat tcggcgatac cgtcgagatc cgttcactaa
     6661 tcgaatggat ctgtctctgt ctctctctcc accttcttct tctattcctt cgggcctgtc
     6721 gggtcccctc ggggttggga ggtgggtctg aaacgataat ggtgaatatc cctgcctaac
     6781 tctattcact atagaaagta cagcaaaaac tattcttaaa cctaccaagc ctcctactat
     6841 cattatgaat aattttatat accacagcca atttgttatg ttaaaccaat tccacaaact
     6901 tgcccattta tctaattcca ataattcttg ttcattcttt tcttgctggt tttgcgattc
     6961 ttcaattaag gagtgtatta agcttgtgta attgttaatt tctctgtccc actccatcca
     7021 ggtcgtgtga ttccaaatct gttccagaga tttattactc caactagcat tccaaggcac
     7081 agcagtggtg caaatgagtt ttccagagca accccaaatc cccaggagct gttgatcctt
     7141 taggtatctt tccacagcca ggattcttgc ctggagctgc ttgatgcccc agactgtgag
     7201 ttgcaacaga tgctgttgcg cctcaatagc cctcagcaaa ttgttctgct gctgcactat
     7261 accagacaat aattgtctgg cctgtaccgt cagcgtcatt gacgctgcgc ccatagtgct
     7321 tcctgctgct cccaagaacc caaggaacaa agctcctatt cccactgctc ttttttctct
     7381 ctgcaccact cttctctttg ccttggtggg tgctactcct aatggttcaa tttttactac
     7441 tttatattta tataattcac ttctccaatt gtccctcata tctcctcctc caggtctgaa
     7501 gatcagcggc cggccgcttg ctgtgcggtg gtcttacttt tgttttgctc ttcctctatc
     7561 ttgtctaaag cttccttggt gtcttttatc tctatccttt gatgcacaca atagagggtt
     7621 gctactgtat tatataatga tctaagttct tctgatcctg tctgaaggga tggttgtagc
     7681 tgtcccagta tttgtctaca gccttctgat gtttctaaca ggccaggatt aactgcgaat
     7741 cgttctagct ccctgcttgc ccatactata tgttttaatt tatatttttt ctttccccct
     7801 ggccttaacc gaattttttc ccatcgcgat ctaattctcc cccgcttaat actgacgctc
     7861 tcgcacccat ctctctcctt ctagcctccg ctagtcaaaa tttttggcgt actcaccagt
     7921 cgccgcccct cgcctcttgc cgtgcgcgct tcagcaagcc gagtcctgcg tcgagagagc
     7981 tcctctggtt tccctttcgc tttcaagtcc ctgttcgggc gccactgcta gagattttcc
     8041 acactgacta aaagggtctg agggatctct agttaccaga gtcacacaac agacgggcac
     8101 acactacttg aagcactcaa ggcaagcttt attgaggctt aagcagtggg ttccctagtt
     8161 agccagagag ctcccaggct cagatctggt ctaaccagag agacccagta caggcaaaaa
     8221 gcagctgctt atatgcagga tctgagggct cgccactccc cagtcccgcc caggccacgc
     8281 ctccctggaa agtccccagc ggaaagtccc ttgtagcaag ctcgatatca gcagttcttg
     8341 aagtactccg gatgcagctc tcgggccacg tgatgaaatg ctaggcggct gtcaaacctc
     8401 cactctaaca cttctctctc cgggtcatcc atcccatgca ggctcacagg gtgtaacaag
     8461 ctggtgttct ctcctttatt ggcctcttct accttatctg gctcaactgg tactagcttg
     8521 tagcaccatc caaaggtcag tggatatctg acccctggcc ctggtgtgta gttctgctaa
     8581 tcagggaagt agccttgtgt gtggtagatc cacagatcaa ggatatcttg tcttctttgg
     8641 gagtgaatta gcccttccaa ctactaagtt tgtagtacat atttaacaaa tacaatttct
     8701 ttaaaatgaa aataattcag aggaatcaca ggtttagagt aaatgaaacc acaggtaatt
     8761 ggcagtggta atagggtatg gggtgggaag tttgggatga ttttggttag cttgagttat
     8821 ccagttgatc cagacatgat aagatacatt gatgagtttg gacaaaccac aactagaatg
     8881 cagtgaaaaa aatgctttat ttgtgaaatt cgtaatcatg tcatagctgt ttcctgtgtg
     8941 aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc
     9001 ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt
     9061 ccagtcggga aacctgtcgt gccacgggga gctttttgca aaagcctagg cctccaaaaa
     9121 agcctcctca ctacttctgg aatagctcag aggccgaggc ggcctcggcc tctgcataaa
     9181 taaaaaaaat tagtcagcca tgagcttgct gcattaatga atcggccaac gcgcggggag
     9241 aggcggtttg cg
//
