LOCUS       sequence-346585-        9450 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector expressing N-terminally tagged APEX2-FLAG-LGALS8.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9450)
  AUTHORS   Eapen VV, Swarup S, Hoyer MJ, Paulo JA, Harper JW
  TITLE     Quantitative proteomics reveals the selectivity of ubiquitin-binding
            autophagy receptors in the turnover of damaged lysosomes by 
            lysophagy.
  JOURNAL   eLife. 2021 Sep 29;10. pii: 72328. doi: 10.7554/eLife.72328.
  PUBMED    34585663
REFERENCE   2  (bases 1 to 9450)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "eLife."; 
            date: "2021-09-29"; volume: "10. pii: 72328. doi"; pages: " 
            10.7554/eLife.72328"
COMMENT     Sequence Label: pHAGE-FLAG-APEX2-LGALS8
FEATURES             Location/Qualifiers
     source          1..9450
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2062..2179
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        2231..2534
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2535..2738
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2689..2709
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2735..2759
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             2847..2870
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             2871..3617
                     /codon_start=1
                     /product="soybean ascorbate peroxidase, engineered to serve
                     as a monomeric tag for electron microscopy and live-cell 
                     proteomics (Lam et al., 2015)"
                     /label=APEX2
                     /note="more active than the original APEX due to the A134P 
                     mutation"
                     /translation="GKSYPTVSADYQDAVEKAKKKLRGFIAEKRCAPLMLRLAFHSAGT
                     FDKGTKTGGPFGTIKHPAELAHSANNGLDIAVRLLEPLKAEFPILSYADFYQLAGVVAV
                     EVTGGPKVPFHPGREDKPEPPPEGRLPDPTKGSDHLRDVFGKAMGLTDQDIVALSGGHT
                     IGAAHKERSGFEGPWTSNPLIFDNSYFTELLSGEKEGLLQLPSDKALLSDPVFRPLVDK
                     YAADEDAFFADYAEAHQKLSELGFADA"
     protein_bind    3633..3657
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(4606..4630)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    4643..5210
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    4660..5212
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(4804..4821)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5031..5050
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             5224..5823
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5224..5243)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5720..5740
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    5840..6428
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5893..5913)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6311..6322)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6503..6736
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(6855..6873)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6941..7045
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7046..7114,7115..7906)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7264..7283)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8077..8665
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8566..8585
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8819..8836
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9076..9097
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9112..9117,9118..9135,9136..9142)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9150..9166
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9155..9177
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9174..9190
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9174..9190
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     9240..9259
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttaattcaca
     2041 aatggcagta ttcatccaca attttaaaag aaaagggggg attggggggt acagtgcagg
     2101 ggaaagaata gtagacataa tagcaacaga catacaaact aaagaattac aaaaacaaat
     2161 tacaaaaatt caaaattttc gggtttatta cagggacagc agagatccag tttggactag
     2221 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
     2281 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
     2341 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
     2401 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
     2461 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
     2521 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
     2581 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
     2641 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
     2701 gtaggcgtgt acggtgggag gtctatataa gcagagctcg tttagtgaac cgtcagatcg
     2761 cctggagacg ccatccacgc tgttttgacc tccatagaag acaccggcgg ccgcagatct
     2821 ctcgaggttg atctgccacc atgatggact acaaggatga cgacgataag ggaaagtctt
     2881 acccaactgt gagtgctgat taccaggacg ccgttgagaa ggcgaagaag aagctcagag
     2941 gcttcatcgc tgagaagaga tgcgctcctc taatgctccg tttggcattc cactctgctg
     3001 gaacctttga caagggcacg aagaccggtg gacccttcgg aaccatcaag caccctgccg
     3061 aactggctca cagcgctaac aacggtcttg acatcgctgt taggcttttg gagccactca
     3121 aggcggagtt ccctattttg agctacgccg atttctacca gttggctggc gttgttgccg
     3181 ttgaggtcac gggtggacct aaggttccat tccaccctgg aagagaggac aagcctgagc
     3241 caccaccaga gggtcgcttg cccgatccca ctaagggttc tgaccatttg agagatgtgt
     3301 ttggcaaagc tatggggctt actgaccaag atatcgttgc tctatctggg ggtcacacta
     3361 ttggagctgc acacaaggag cgttctggat ttgagggtcc ctggacctct aatcctctta
     3421 ttttcgacaa ctcatacttc acggagttgt tgagtggtga gaaggaaggt ctccttcagc
     3481 taccttctga caaggctctt ttgtctgacc ctgtattccg ccctctcgtt gacaaatatg
     3541 cagcggacga agatgccttc tttgctgatt acgctgaggc tcaccaaaag ctttccgagc
     3601 ttgggtttgc tgatgccgct cgaggttatc aaacaagttt gtacaaaaaa gttggcatgt
     3661 tgtccttaaa caacctacag aatatcatct ataacccggt aatcccgttt gttggcacca
     3721 ttcctgatca gctggatcct ggaactttga ttgtgatacg tgggcatgtt cctagtgacg
     3781 cagacagatt ccaggtggat ctgcagaatg gcagcagcat gaaacctcga gccgatgtgg
     3841 cctttcattt caatcctcgt ttcaaaaggg ccggctgcat tgtttgcaat actttgataa
     3901 atgaaaaatg gggacgggaa gagatcacct atgacacgcc tttcaaaaga gaaaagtctt
     3961 ttgagatcgt gattatggtg ctgaaggaca aattccaggt ggctgtaaat ggaaaacata
     4021 ctctgctcta tggccacagg atcggcccag agaaaataga cactctgggc atttatggca
     4081 aagtgaatat tcactcaatt ggttttagct tcagctcgga cttacaaagt acccaagcat
     4141 ctagtctgga actgacagag ataagtagag aaaatgttcc aaagtctggc acgccccagc
     4201 ttaggctgcc attcgctgca aggttgaaca cccccatggg ccctggacga actgtcgtcg
     4261 ttaaaggaga agtgaatgca aatgccaaaa gctttaatgt tgacctacta gcaggaaaat
     4321 caaaggatat tgctctacac ttgaacccac gcctgaatat taaagcattt gtaagaaatt
     4381 cttttcttca ggagtcctgg ggagaagaag agagaaatat tacctctttc ccatttagtc
     4441 ctgggatgta ctttgagatg ataatttatt gtgatgttag agaattcaag gttgcagtaa
     4501 atggcgtaca cagcctggag tacaaacaca gatttaaaga gctcagcagt attgacacgc
     4561 tggaaattaa tggagacatc cacttactgg aagtaaggag ctggtaccca actttcttgt
     4621 acaaagtggt tcgataacga attccgcccc ccccccctaa cgttactggc cgaagccgct
     4681 tggaataagg ccggtgtgcg tttgtctata tgttattttc caccatattg ccgtcttttg
     4741 gcaatgtgag ggcccggaaa cctggccctg tcttcttgac gagcattcct aggggtcttt
     4801 cccctctcgc caaaggaatg caaggtctgt tgaatgtcgt gaaggaagca gttcctctgg
     4861 aagcttcttg aagacaaaca acgtctgtag cgaccctttg caggcagcgg aaccccccac
     4921 ctggcgacag gtgcctctgc ggccaaaagc cacgtgtata agatacacct gcaaaggcgg
     4981 cacaacccca gtgccacgtt gtgagttgga tagttgtgga aagagtcaaa tggctctcct
     5041 caagcgtatt caacaagggg ctgaaggatg cccagaaggt accccattgt atgggatctg
     5101 atctggggcc tcggtgcaca tgctttacat gtgtttagtc gaggttaaaa aaacgtctag
     5161 gccccccgaa ccacggggac gtggttttcc tttgaaaaac acgatgataa tatggccaca
     5221 accatgaccg agtacaagcc cacggtgcgc ctcgccaccc gcgacgacgt ccccagggcc
     5281 gtacgcaccc tcgccgccgc gttcgccgac taccccgcca cgcgccacac cgtcgatccg
     5341 gaccgccaca tcgagcgggt caccgagctg caagaactct tcctcacgcg cgtcgggctc
     5401 gacatcggca aggtgtgggt cgcggacgac ggcgccgcgg tggcggtctg gaccacgccg
     5461 gagagcgtcg aagcgggggc ggtgttcgcc gagatcggcc cgcgcatggc cgagttgagc
     5521 ggttcccggc tggccgcgca gcaacagatg gaaggcctcc tggcgccgca ccggcccaag
     5581 gagcccgcgt ggttcctggc caccgtcggc gtctcgcccg accaccaggg caagggtctg
     5641 ggcagcgccg tcgtgctccc cggagtggag gcggccgagc gcgccggggt gcccgccttc
     5701 ctggagacct ccgcgccccg caacctcccc ttctacgagc ggctcggctt caccgtcacc
     5761 gccgacgtcg aggtgcccga aggaccgcgc acctggtgca tgacccgcaa gcccggtgcc
     5821 tgatgcatcg atggatccta atcaacctct ggattacaaa atttgtgaaa gattgactgg
     5881 tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta
     5941 tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct
     6001 gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt
     6061 tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac
     6121 tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg
     6181 ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaaatcatc
     6241 gtcctttcct tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg
     6301 ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct
     6361 gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt cggatctccc tttgggccgc
     6421 ctccccgcct gagatccttt aagaccaatg acttacaagg cagctgtaga tcttagccac
     6481 tttttaaaag aaaagggggg actggaaggg ctaattcact cccaacgaag acaagatctg
     6541 ctttttgctt gtactgggtc tctctggtta gaccagatct gagcctggga gctctctggc
     6601 taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg
     6661 tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg
     6721 tggaaaatct ctagcagtag tagttcatgt catcttatta ttcagtattt ataacttgca
     6781 aagaaatgaa tatcagagag tgagaggccc gggttaatta aggaaagggc tagatcattc
     6841 ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat
     6901 ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt
     6961 atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct
     7021 tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc
     7081 cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa
     7141 agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg
     7201 taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt
     7261 tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg
     7321 catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac
     7381 ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc
     7441 ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa
     7501 catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc
     7561 aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt
     7621 aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga
     7681 taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa
     7741 atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa
     7801 gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa
     7861 tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt
     7921 ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt
     7981 gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg
     8041 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt
     8101 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca
     8161 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac
     8221 tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac
     8281 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct
     8341 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg
     8401 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca
     8461 gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt
     8521 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta
     8581 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc
     8641 gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc
     8701 cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa
     8761 ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag
     8821 cgagtcagtg agcgaggaag cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg
     8881 ttggccgatt cattaatgca gcaagctcat ggctgactaa ttttttttat ttatgcagag
     8941 gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc
     9001 ctaggctttt gcaaaaagct ccccgtggca cgacaggttt cccgactgga aagcgggcag
     9061 tgagcgcaac gcaattaatg tgagttagct cactcattag gcaccccagg ctttacactt
     9121 tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc acacaggaaa
     9181 cagctatgac atgattacga atttcacaaa taaagcattt ttttcactgc attctagttg
     9241 tggtttgtcc aaactcatca atgtatctta tcatgtctgg atcaactgga taactcaagc
     9301 taaccaaaat catcccaaac ttcccacccc ataccctatt accactgcca attacctgtg
     9361 gtttcattta ctctaaacct gtgattcctc tgaattattt tcattttaaa gaaattgtat
     9421 ttgttaaata tgtactacaa acttagtagt
//
