LOCUS       sequence-346586-       11773 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pNOC episomal plasmid harboring the dead version of humanized 
            fnCas12a gene sequence tagged to the KRAB domain and Nlux and crRNA 
            targeting the fluorescent gene tdtomato with spacer 2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11773)
  AUTHORS   Naduthodi MIS, Sudfeld C, Avitzigiannis EK, Trevisan N, van Lith E, 
            Alcaide Sancho J, D'Adamo S, Barbosa M, van der Oost J
  TITLE     Comprehensive Genome Engineering Toolbox for Microalgae 
            Nannochloropsis oceanica Based on CRISPR-Cas Systems.
  JOURNAL   ACS Synth Biol. 2021 Dec 17;10(12):3369-3378. doi: 
            10.1021/acssynbio.1c00329. Epub 2021 Nov 18.
  PUBMED    34793143
REFERENCE   2  (bases 1 to 11773)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.1c00329"; journalName: "ACS Synth Biol"; date: 
            "2021-12-17- 17"; volume: "10"; issue: "12"; pages: "3369-3378"
COMMENT     Sequence Label: pNOC_dfnCas12a-KRAB_crRNA Td2
FEATURES             Location/Qualifiers
     source          1..11773
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        312..416
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(417..485,486..1277)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(635..654)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1448..2036
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1937..1956
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     regulatory      3541..3550
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(4232..4252)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     regulatory      4257..4266
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(4268..4294)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             complement(4976..5170)
                     /codon_start=1
                     /product="Kruppel-associated box (KRAB) transcriptional 
                     repression domain from the human zinc finger protein ZNF10 
                     (Margolin et al., 1994)"
                     /label=KRAB
                     /translation="RTLVTFKDVFVDFTREEWKLLDTAQQIVYRNVMLENYKNLVSLGY
                     QLTKPDVILRLEKGEEPWLV"
     CDS             complement(9119..9139)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(9360..9371)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     ncRNA           10368..10404
                     /product="self-cleaving hammerhead ribozyme (Ferre-D'Amare 
                     and Scott, 2010)"
                     /label=hammerhead ribozyme
                     /ncRNA_class="hammerhead_ribozyme"
     ncRNA           10449..10516
                     /product="hepatitis delta virus self-cleaving ribozyme 
                     (Ferre-D'Amare and Scott, 2010)"
                     /label=HDV ribozyme
                     /ncRNA_class="ribozyme"
     misc_feature    join(11545..11773,1..275)
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
ORIGIN
        1 tagatataca ttcgattaac gataagtaaa atgtaaaatc acaggatttt cgtgtgtggt
       61 cttctacaca gacaagatga aacaattcgg cattaatacc tgagagcagg aagtacaaga
      121 taaaaggtag tatttgttgg cgatccccct agagtctttt acatcttcgg aaaacaaaaa
      181 ctattttttc tttaatttct ttttttactt tctattttta atttatatat ttatattaaa
      241 aaatttaaat tataattatt tttatagcac gtgatgaaaa ggacccaggt ggcacttttc
      301 ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc
      361 cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga
      421 gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt
      481 ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag
      541 tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag
      601 aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta
      661 ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg
      721 agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca
      781 gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag
      841 gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc
      901 gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg
      961 tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc
     1021 ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg
     1081 cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg
     1141 gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga
     1201 cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac
     1261 tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa
     1321 aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca
     1381 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag
     1441 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
     1501 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
     1561 ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc
     1621 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
     1681 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
     1741 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
     1801 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc
     1861 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca
     1921 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
     1981 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
     2041 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct
     2101 ttcctgcgtt atcccctgat tctgtggttt aaaccaggtc actggatttt ggttttagga
     2161 attagaaatt ttattgatag aagtatttta caaatacaaa tacatactaa gggtttctta
     2221 tatgctcaac acatgagcga aaccctataa gaaccctaat tcccttatct gggaactact
     2281 cacacattat tctggagaaa aatagagaga gatagatttg tagagagaga ctggtgattt
     2341 ttgcggactc cggtcggcat ctactactag ttcaagcgct ctcccacacg tagcccgaag
     2401 ggagaagttc tctgataccg accgccgtag gctgcccgtc ggaatccttc acaatcgcct
     2461 tgatcccagg gtggaggtcg agcaggacct gccggcaccg cccgcagggg ctcagaatcc
     2521 cgcggttctc gttcccgatg gcgacaatgc acgtcaggtt cccagccgcg gcagcagcgg
     2581 cggtccccag gaccaccagc tccgcgcaag gcccccccgt aaaatggtac acgttgaccc
     2641 ccgtgaagat ccgtccgtcg gacgacagag cagcggaagc caccgagtaa tcctcggaga
     2701 taggaatcga gttaatcgtc gccgtagccc tctcgatcag cgtgctctcc tcctgcgaga
     2761 ggggcatggg ccctcctgtg ttgatgcggg ctgagattgg tggtggtcta tcacgaatat
     2821 gtgtgagggg taagtgcggt gttttgcgtg agattttaga atattgcccc gccccggggc
     2881 aggccggcgt ggcggaacaa ccaggcacac gagcgcgaat ggtgataccg acggagtcaa
     2941 aactttgtga caagtagctg caccatgggc agtggtgagc tttcagacgt ggtatcactg
     3001 tccactagtt cacacacaga atgcgtgtcc aaaaggtcta gagccgtctc gcttgcgtct
     3061 ctccgtcgaa gaacagtgaa gaggctcgtc acgtcgacca gacgacggga ggctggtcac
     3121 catcgcagat gtctcccaca aagcagcacg gcaactccta ctccttcaca caatggaaga
     3181 aaaggtggtc tgatggttct cagtggaaaa gaacgatatc aggctgaaaa aaatgatctg
     3241 caggctccag attcctgaat cacgtcgact gtgacgaagc aaaccgcgtc gaacaacatc
     3301 ggtcatgcca acgggtctcg tctctcgagc ccttttggcg gcgactaaga agtatgaagc
     3361 ttcaggccgc aacgcgcgac acagcgtttt gtgtggtggg cctcggcatt gctctttgca
     3421 tggcccagcg tgattagtgc gtggatttta agcccgagac cgaaggattg cgacatgtgc
     3481 ctggctgtat aactcacgct tgctgctacg ctcgcctcct cctccatcca ctccatcgcg
     3541 gccgccatgg cctctagtgg atcagcttgc atgcctgcag gagacgatat cacctcttct
     3601 gtttccacga taaaaataga ctgctcattt cttcgtcgtc ttcatcgtct gctttttctg
     3661 cttcgcctct gtctggggtc tgaaaccact acacacacac acaacactcg tactcccact
     3721 ttcacaaaag cgtaagctca ccggcttttc ttacacgtac attttagtgg atcccatcac
     3781 gccactacca cgcccgcggg ggatggaacg gaggggagag agagaagggg gaagcatgga
     3841 tgaatgagac attgagggaa aggaggggag ggagcagtcc atcagggcgc tacctctctt
     3901 gtcccccaaa ccctgttgag ccgttcaaca tgtttcatgt ttcctccctc cccccttccc
     3961 tccctggcct ttccgcggag ccattcaagt gacgtctgga ccgcaccgta acaaaatcgt
     4021 ttctatgggg ggtttgtttg acaaccacgt cttcagcgtt tttaaaaaaa aaaagcgggc
     4081 aagccctctc accctcactc atgcccatcc tcctcctctc ctgcggaaca ttcttacaaa
     4141 aggcgtaact cgacgacaac tcaaagaacg acaaacatca atcccaaaaa aaaaatctct
     4201 actcgtctct cttggatctt tccaattgtc agaccttcct cttctttttc gggctagcca
     4261 ccatggacgc gtagtcgggc acgtcgtagg ggtatccggc caggatgcgc tcgcacaggc
     4321 gccagccggt taccccgttg atggtcacgc ggaacaacag cgagccatcc gggttgatca
     4381 gccgctcgtc aatgatctta ttgccgttcc acagggtgcc ggtcaccgtg atcttcttgc
     4441 cgtcgaacac ggcaatgccc tcgtaaggac ggccgaagta gtcaatcatg ttcggcgtca
     4501 caccgtcgat gaccagcgtc ccgtagtgca gtatcacctt gaagtggtga tcgtccacgg
     4561 ggtacacaac cttgaagatc ttctcgatct ggcccatttg gtccccgctc aggccctcgt
     4621 agggaatgat gacgtggatg tcgatcttca ggccgttctc gccgctcagg acgatgcgct
     4681 ggattggggt cacggagacg cccaggttct ggaaaagcga ggacacaccg ccctgctcca
     4741 gcacctggtc gaggttatag ccagctgtct gccgccagtc gcccacgaag tcctcgagag
     4801 taaacaccat gttaacggac ccgctgccgg acccccacaa gtcgttacgg gccataccct
     4861 ccatcttgat atcgcaagac tgcttgtcct taaaaatgct acgagaagag actgaagatt
     4921 taatttcaaa agctgtttca gaatcagggt gggtttcttg atgaatttcc ctctcaacta
     4981 gccagggttc ttcgcctttc tctaatctca gtatgacatc tggcttggtc agttgataac
     5041 caagggaaac taaatttttg tagttttcca acataacgtt cctgtaaaca atttgctggg
     5101 cagtgtcaag tagtttccac tcctcacgag taaagtccac gaaaacatcc ttaaatgtga
     5161 ccaacgttct gctccaggct gtcaaagact tcgcgtcgtt aacggacccg ctgccggacc
     5221 cgttatttct attctggaca aactcgaagt attcctcgtt cttaatgacc aggttcagct
     5281 tcttcccctc ctgattgttc ttgatccggc ccagcagcat cagtcccttc agcccgatgt
     5341 ggtaggcccc gttggcgtct gcatcctgag gcatattttt gggtgcctgt ctgctgtcga
     5401 agaagtttcc gttgacatca gccacggggc taatcagata gtccagttct gtcccggtct
     5461 ttgagttccg catctgcagg attgtattca gcactgaggt cagcttggcg aaaaatttct
     5521 tatcactctc gccacagatg gctgccttga tgcattcgcc atgtccatac tcgatagagt
     5581 agtctttcag cagcttttcc agctccttgg ttgggtacac ctccctagtg tcccaattgt
     5641 ggtttttatc ggaatttcga aagttgatca ggcgagatcc gaaggaggca atggtccatt
     5701 tccccttagc ggccttgtcg ccgaagttct tgtaatcgaa ggaaaactcg aagtacccct
     5761 tgtccagatt ataacagatc ttatcgaact tgctgaaaaa ttcctgggac ttgctcactg
     5821 actcatattt agggtacagc tggttgacaa agccggtcac ggggcagatc ttggaagtga
     5881 atccggctgg cacatagtag atgatgcctg tctgttttcc cattttcttg aaagtttcaa
     5941 agggagctgt cagctggtag gctctcagga cgcctccggt cttgtcgaac tcgttatctt
     6001 taaacaccag gtaattcagc ttttcgatca gcattttctc cagcttctga tagacctgtt
     6061 tttccacctt aaagcgcccc ctcttaaagc cgaagttcag atccgcgaac accacaatgg
     6121 cattgtattc gatgaccagc tttgcaatct catggaccac ctggctcaga tagccttcct
     6181 tcatctcctt aatgttgttg attttcttcc agtccttcct agctgagtct ctatccttct
     6241 cgattgcagc cagtttatcg tggtagttgg ttttcatccg gtcattgcca atgatgttga
     6301 atgtatcctg cttaatgata ttccctttgc catccaccag ggtatagtag gccagatgcc
     6361 gctctcctcg ggcaatgctc aggatgtgca catcgtttgc tttttccttc agcagcagat
     6421 tgatctcgtc gttgaacttg ttagcgccgc ttgacttgaa gttaatggtg attggacaat
     6481 ggaaaaagaa cttatcttcg gtgaaccgct tgtccttaat cagatcgtat tcgaacacgc
     6541 tctctttctt aggattgtcc ttgttcttgt tagcgatggc ctccttagct gggtgagtga
     6601 ttttcttagg aatagactgc ttccggtaaa acagttcggc ctctccgttc agtttataga
     6661 ccacgtcctg cagattcctc tcatcgaaca gggccttcca gtacagtgta tgcaggtttg
     6721 gtctgccctt gctgtatgct gaaaaatctt tgttatagat ctggaacagg tacagctttc
     6781 cctgattgac cacgctgtcg atgtaacttt cagaaatgtt ctcaaaagtc agcttatacc
     6841 cctgattttc cacctcgcgg tagaactcgt cgatagagtt atatctctgt gtgtcggaaa
     6901 accggaagcc aaaatccttc cattcagggt gtttggagat gctctgcttg tagaagtcaa
     6961 taaacttcct gcaatcctcg atgttgaact caaatttctc atatcccttc tgggggctcc
     7021 cgttcttagt atgtgttgag tgatttctaa tccgcaggat gtcctcggat gggttgtaga
     7081 atttgatact cttggcgctg aagaacacct taggcagcat cttatttgcg ccgggcagca
     7141 gcttatacac gattttcttg tatccttccc ctttgttctc cttaatggct ttgtcatcga
     7201 agatcttatt gtttttctta ttcatcactc ccagatagta cttgtcatcc ttgatgaaca
     7261 ggatagctgt gttgtcaggc tccttgttct tatcccaccc gttggccagg gtactatttt
     7321 cgaagttcag tttaaacttc tcgtcagagt aaggcttctg tgtgatgtag ttcctaatct
     7381 tgttatacag ggggacaatg tttgccagct cgaagtaaca ttcctcgaac accaggtaga
     7441 agtgttcgtc tttatccagg atattagcct tatcctctga ctgactaata tggaagattt
     7501 tcagcttgtg cagcagattg ttggtctggt ccagcagatc cttgatggct ttcacgtcat
     7561 cttctgctga agcctgcagc aggtctttct tgccctggtt ctggtactta atactgatct
     7621 gagccagatt gtctttgttc tgggcgatct catcaaaaat catggggatg gctgcgaagt
     7681 tggccaggat ttcctcaaag cggcactgtt tgtcaatatc cctgtgcttg ttgaactcct
     7741 ccagtgccag cttaattgtt tccagactca gatattttgc cttctcggtt ttcttagcga
     7801 tcagttcctg ctctttctta gagggattat ccaggttctt tggagcgatc tgctgtgtaa
     7861 tgtactccag gacggcggtc ccaatcacgg aatagtcatc aaacacctgc tgtgacaggt
     7921 ctgtcagact cttatcgttt ttgaagtaga tcttactcag gtccagcttc tgggctttca
     7981 ggtcatcgaa cagcagactc agagtttcct taatagattt ttcctccact gtcttgaaag
     8041 cggcgatctg ctcataaaag ctctgcatgg tagtgaccac gtctgagtca tcttccagtt
     8101 tatcaatgac aaaagacttg gactcggtat cactcaggat ctgtttgaac agcacagaca
     8161 tcttgtattt cttcagagtt ttgtcgttga tctgctggct atacagattg atgtattcgt
     8221 taattccctt gcgcttggta ttctccccgt tcacaaactt cccgccaatg atggtattga
     8281 atttagtaat gccggactgg ttcaggtaat tgttgaaatt ggcgatttca aacacctcgt
     8341 ccagggaaaa gaccctctgg ttcacctcgc tagtcttata gtcgatatcg aatgtcagtt
     8401 cctcagccag atctttcttg atctgttcgt aattaattgc ctcgggagct ttgtccttca
     8461 gagattcata tttggccttg ttctccagga actttggcag attgtcatcg acgattcggt
     8521 agatgatgga ggtaggaatg tcgttgctgg agtacacgtt cttgcggttc tcgtggaagc
     8581 ctttaaagta tgtggtccat cccttgaagc tcttgatgat ttccagtgcc tcgtcaatat
     8641 cagtgatatc ggagttagcc ttgaacagtt caatcccatt gtccttagac tgtttcagcc
     8701 acaggatcag gtcggactcc tggcctttct tagcatcgat cagattctgg ttgaacagat
     8761 ttttaaactt ttcggagtcc ttgatgtact cgctaatctg tttcttgatg gtgtccttgg
     8821 cggacttgaa gtccttctgc aggttgtcat cgtcgctctt cttcagcttg aagtacacgt
     8881 ctgagtaatt ctgcagcaga tcctcactga tgcacacgga gctcagaatt tcctcgataa
     8941 agaactggtg gtatttatca atgatctgct tggctttctt atagtctttt gcccgcttct
     9001 catcgtccag aatcaggcct cgggccttga tgttctccag tgtcttgccc tgtgggatca
     9061 gctcgaaccg cagtgtctta ctcagtgaat acttgttgac gaactcctgg tagatgctca
     9121 ccttccgctt tttcttgggc atgctcgagg gttgcgtgtg tatctgtgtg cagtggatat
     9181 tgttaccgag tttggtgagc gtgagtccgt tagtgccctg gtggtggtgg attaggagag
     9241 tgggtgactc ggtgtccatg gctttcttcg ctcattatag gaggggaaag gaatgaggga
     9301 gggtggggag acagcgtctg ttgttgacca ccgatttact tcttgcctcc cttcccctcc
     9361 ctcccctcaa tccgtacgac acaaatagta gccgagtgtc tgctgcagag cgcatgatta
     9421 gtgtggtaga caacgaggga gggaaggatg tacagggcat ggcacggaga agcgatggtg
     9481 gccaggaaga ggagaggtcg cgagaacagg atgtgttgcg aatggataaa aacagaaagc
     9541 gatggctctg ggcttcgaaa gcaggggaca ttaggacgtg tagaccatct cgacggatcc
     9601 ctctgtatct ctgttgtgcg tgaatgtttt ctgtgcacgt gtagtgtgtg agagtagaac
     9661 ccgggaactc gaacagagaa aagcatgggt ggctgtggtg tggaggcttc gttcccacca
     9721 catgcccttc tccttcgcct cgcctctccc tgccttcttc cacgcaccct tgcgcccctc
     9781 gttctcaata cctggctcac ttccaccatt caaacaacca tcacgataca ggcatttatc
     9841 tatcgttgaa gacttcttcc tccggtagat cttagccaag gtaagaagag gggcatgcag
     9901 caaggagaaa gaaatgatgc atgatgagga atagaagggg aggagggagg gatatgatgg
     9961 gaagcgaaag cgcatattct ggtggtctgc tgcctgatgg ggacgcgtct agctgtgaca
    10021 ctgaggacgg tggctgctgg tggctgcggg cgctgccttg gtgatcaatg ggagtaaagg
    10081 gagggaagga ggtagcgtga acggatgacg cggagaagtt taggggtctc tttacgtatc
    10141 gcccctgccg cccgcctctc tgcgataaat gtgcctgtta ccctgcagcc tctattcttc
    10201 actgtgttcc tgttttccaa cagcctctat tcttccctgt cttttgttgc agtggcgtca
    10261 tcctctcttt gccccagtcg tcgttctctc gactcactca ctccccccct ccttccctcc
    10321 ctccatccac agaatcgatg aagagcaggc cttggctctt cgaaattctg atgagtccgt
    10381 gaggacgaaa cgagtaagct cgtcaatttc tactgttgta gatatgacgg ccatgttgtt
    10441 gtcctcgggg ccggcatggt cccagcctcc tcgctggcgc cggctgggca acatgcttcg
    10501 gcatggcgaa tgggactcct gggtaccatg ggaaagaaag gatgagaaag gagaaaggac
    10561 atctagataa ccggcatatc acggtggtgt attagtgtag aatagtgaag agaagacttg
    10621 ggaaaatgtg taggaaaggt tgtttctgtg tatgtgggtt gggatgggtg gctgtttgag
    10681 aaggaacagc gggcagggcg atgtagtgct gaacgggcac ggaaccactg agactgaagg
    10741 aagtagggag agagaggggc aggggacgtg cactttaatc tttgcctcgg tagagtatac
    10801 ccatgcaaga gtatgtggcc acctgtggtg gcttttggcc aggtctggtg cagtgccaat
    10861 catctcccat caataataca acttcagaac aacggcgcat tgatggggag agagaaagta
    10921 aatttaagta aggggtacgt agtagaggat tcaactgaaa tattttcgag gagcggttgg
    10981 gaagttgacc gattgaaagg agaagggagg ggagcaggtg tgatagtcat gtgtaaagta
    11041 attctttttt gccgtcgtca cacaatccac atcaatgata aaatatgttt aaggatcaat
    11101 cacacggagt cggtcataag gcaaccgcaa acgcaatgca aactagcaag caagcaggac
    11161 cacaacaaca accattacca tcacaggcga cagcagcagc agctgcagca gcagcaaggc
    11221 aagcaaaggc catctgcgcg tggacttttc caggcaccgg ctttaagcgt aattttcaat
    11281 gattgttgtc cgtgtatcct ctcacccttt caccgcttgc gcgcacgtga gcagcacaac
    11341 tggtaatgcc cgaggacaat agacgaggaa aaagaagaag aaaaatgaag agggagtgat
    11401 gaagaagaag agaagaataa aagaagacag aaaagaagat aaaaaagtca aaagacgaca
    11461 aagacggaaa aaaaagaaac gcgcaatttt aacaccagca acgacgacga aaagggcgtc
    11521 agctttgcac cggtttctta gacggatcgc ttgcctgtaa cttacacgcg cctcgtatct
    11581 tttaatgatg gaataatttg ggaatttact ctgtgtttat ttatttttat gttttgtatt
    11641 tggattttag aaagtaaata aagaaggtag aagagttacg gaatgaagaa aaaaaaataa
    11701 acaaaggttt aaaaaatttc aacaaaaagc gtactttaca tatatattta ttagacaaga
    11761 aaagcagatt aaa
//
