LOCUS       sequence-346592-        9840 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector expressing N-terminally tagged 
            APEX2-FLAG-CALCOCO2/NDP52.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9840)
  AUTHORS   Eapen VV, Swarup S, Hoyer MJ, Paulo JA, Harper JW
  TITLE     Quantitative proteomics reveals the selectivity of ubiquitin-binding
            autophagy receptors in the turnover of damaged lysosomes by 
            lysophagy.
  JOURNAL   eLife. 2021 Sep 29;10. pii: 72328. doi: 10.7554/eLife.72328.
  PUBMED    34585663
REFERENCE   2  (bases 1 to 9840)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "eLife."; 
            date: "2021-09-29"; volume: "10. pii: 72328. doi"; pages: " 
            10.7554/eLife.72328"
COMMENT     Sequence Label: pHAGE-FLAG-APEX2-CALCOCO2
FEATURES             Location/Qualifiers
     source          1..9840
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(13..30)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(184..772)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(264..283)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(943..1734,1735..1803))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1566..1585
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1804..1908)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     1976..1994
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     LTR             2113..2346
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     misc_feature    2421..3009
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             2527..2538
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     2936..2956
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3026..3625)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(3109..3129)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     3606..3625
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     misc_feature    3637..4189
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    3639..4206
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(3799..3818)
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     primer_bind     4028..4045
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     protein_bind    4219..4243
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(5582..5606)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(5622..6368)
                     /codon_start=1
                     /product="soybean ascorbate peroxidase, engineered to serve
                     as a monomeric tag for electron microscopy and live-cell 
                     proteomics (Lam et al., 2015)"
                     /label=APEX2
                     /note="more active than the original APEX due to the A134P 
                     mutation"
                     /translation="GKSYPTVSADYQDAVEKAKKKLRGFIAEKRCAPLMLRLAFHSAGT
                     FDKGTKTGGPFGTIKHPAELAHSANNGLDIAVRLLEPLKAEFPILSYADFYQLAGVVAV
                     EVTGGPKVPFHPGREDKPEPPPEGRLPDPTKGSDHLRDVFGKAMGLTDQDIVALSGGHT
                     IGAAHKERSGFEGPWTSNPLIFDNSYFTELLSGEKEGLLQLPSDKALLSDPVFRPLVDK
                     YAADEDAFFADYAEAHQKLSELGFADA"
     CDS             complement(6369..6392)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     primer_bind     complement(6480..6504)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        complement(6501..6704)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(6530..6550)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        6705..7008
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     misc_feature    7060..7177
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(7474..7518)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    7703..7936
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    8433..8558
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             8605..9238
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(9430..9449)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(9499..9515)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9499..9515)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9512..9534)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9523..9539
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9547..9553,9554..9571,9572..9577))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9592..9613
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt
       61 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
      121 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
      181 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
      241 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
      301 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
      361 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
      421 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
      481 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
      541 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
      601 gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt
      661 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
      721 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
      781 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
      841 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt
      901 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag
      961 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt
     1021 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc
     1081 gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc
     1141 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg
     1201 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac
     1261 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg
     1321 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc
     1381 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact
     1441 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc
     1501 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaac
     1561 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc
     1621 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac
     1681 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa
     1741 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact
     1801 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg
     1861 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg
     1921 aaaagtgcca cctgacgtct aagaaaccat tattatcatg acattaacct ataaaaatag
     1981 gcgtatcacg aggccctttc gtcttcaaga atgatctagc cctttcctta attaacccgg
     2041 gcctctcact ctctgatatt catttctttg caagttataa atactgaata ataagatgac
     2101 atgaactact actgctagag attttccaca ctgactaaaa gggtctgagg gatctctagt
     2161 taccagagtc acacaacaga cgggcacaca ctacttgaag cactcaaggc aagctttatt
     2221 gaggcttaag cagtgggttc cctagttagc cagagagctc ccaggctcag atctggtcta
     2281 accagagaga cccagtacaa gcaaaaagca gatcttgtct tcgttgggag tgaattagcc
     2341 cttccagtcc ccccttttct tttaaaaagt ggctaagatc tacagctgcc ttgtaagtca
     2401 ttggtcttaa aggatctcag gcggggaggc ggcccaaagg gagatccgac tcgtctgagg
     2461 gcgaaggcga agacgcggaa gaggccgcag agccggcagc aggccgcggg aaggaaggtc
     2521 cgctggattg agggccgaag ggacgtagca gaaggacgtc ccgcgcagaa tccaggtggc
     2581 aacacaggcg agcagccaag gaaaggacga tgatttcccc gacaacacca cggaattgtc
     2641 agtgcccaac agccgagccc ctgtccagca gcgggcaagg caggcggcga tgagttccgc
     2701 cgtggcaata gggaggggga aagcgaaagt cccggaaagg agctgacagg tggtggcaat
     2761 gccccaacca gtgggggttg cgtcagcaaa cacagtgcac accacgccac gttgcctgac
     2821 aacgggccac aactcctcat aaagagacag caaccaggat ttatacaagg aggagaaaat
     2881 gaaagccata cgggaagcaa tagcatgata caaaggcatt aaagcagcgt atccacatag
     2941 cgtaaaagga gcaacatagt taagaatacc agtcaatctt tcacaaattt tgtaatccag
     3001 aggttgatta ggatccatcg atgcatcagg caccgggctt gcgggtcatg caccaggtgc
     3061 gcggtccttc gggcacctcg acgtcggcgg tgacggtgaa gccgagccgc tcgtagaagg
     3121 ggaggttgcg gggcgcggag gtctccagga aggcgggcac cccggcgcgc tcggccgcct
     3181 ccactccggg gagcacgacg gcgctgccca gacccttgcc ctggtggtcg ggcgagacgc
     3241 cgacggtggc caggaaccac gcgggctcct tgggccggtg cggcgccagg aggccttcca
     3301 tctgttgctg cgcggccagc cgggaaccgc tcaactcggc catgcgcggg ccgatctcgg
     3361 cgaacaccgc ccccgcttcg acgctctccg gcgtggtcca gaccgccacc gcggcgccgt
     3421 cgtccgcgac ccacaccttg ccgatgtcga gcccgacgcg cgtgaggaag agttcttgca
     3481 gctcggtgac ccgctcgatg tggcggtccg gatcgacggt gtggcgcgtg gcggggtagt
     3541 cggcgaacgc ggcggcgagg gtgcgtacgg ccctggggac gtcgtcgcgg gtggcgaggc
     3601 gcaccgtggg cttgtactcg gtcatggttg tggccatatt atcatcgtgt ttttcaaagg
     3661 aaaaccacgt ccccgtggtt cggggggcct agacgttttt ttaacctcga ctaaacacat
     3721 gtaaagcatg tgcaccgagg ccccagatca gatcccatac aatggggtac cttctgggca
     3781 tccttcagcc ccttgttgaa tacgcttgag gagagccatt tgactctttc cacaactatc
     3841 caactcacaa cgtggcactg gggttgtgcc gcctttgcag gtgtatctta tacacgtggc
     3901 ttttggccgc agaggcacct gtcgccaggt ggggggttcc gctgcctgca aagggtcgct
     3961 acagacgttg tttgtcttca agaagcttcc agaggaactg cttccttcac gacattcaac
     4021 agaccttgca ttcctttggc gagaggggaa agacccctag gaatgctcgt caagaagaca
     4081 gggccaggtt tccgggccct cacattgcca aaagacggca atatggtgga aaataacata
     4141 tagacaaacg cacaccggcc ttattccaag cggcttcggc cagtaacgtt aggggggggg
     4201 ggcggaattc gttatcgaac cactttgtac aagaaagttg ggcagagaga gtggcagaac
     4261 acgtggtctt caaagatctg cttctctgta gctgggaaga tcttgtcaca aattggacaa
     4321 ttgaaacaaa ggggctgcat ctgctgttgc tccaaggtgt gatcacaaat atcatctgct
     4381 ttgcagatag ggcatttctt gatggagagc gggctggggg aagaactttc ttggatacca
     4441 gaatatgggt ttccataggc aagtcctgga ttttgtcttg cgcctccttc atctgaagta
     4501 ggtacttgat acggcaaaga attaaaatcc agacccatgt aactgagcaa tctgctgttc
     4561 tccctcttca aaagatcatt ttctccttcc aatctctctt tctgtctctg cagagcatta
     4621 catataattt cgttctcact cagtcttttt gacaggtcaa agttttcatc cattaattcc
     4681 tgttgtttct tcattgcagt agtttcattc tgcttcattt gctccactgt ctgctcgagc
     4741 ttcttctggt ccttcctctg ttcagctaaa ctgagaaaga ggtggtcatt ttcctttttc
     4801 agctgctcta actgctctgt cttatcttga tctccctgaa caagcttctc catttctttc
     4861 tcttgagttg acagctgggc ctgaagctga tccactctga ttcccatctt ctcattttct
     4921 gaggacatct tctggttttg ttctttcagt tgaagcagct ctgtctccca atagtccttc
     4981 tgttctttca ctttcagttc caacttctta ttgatgctct gtagggtttc tagctcctcc
     5041 tgcttctttt ggagctcagc ctgcatgtct gagttctgct tctggaggct gatacagctg
     5101 tccttcagct cctggttttc tttgcaaagc tccttgttgt gctgctcaat ctcttccacc
     5161 tctccctgag tggtaacaac caggatgtct tcctcatttt ctggacggaa ttggaaagga
     5221 atacttgctc cccggaccac accatcctca tccacatagc agaactggta atactcatca
     5281 tccttgggca ggtagtaagc tttgaattgg acttcctgct gtttagctga tttgttgttt
     5341 aggtcaatgg gcaaagtaac ccacatgaag gtgtaatact cacgggttgt cttccacccc
     5401 actctaaaga tgccaatcca atcctttcga cgagggatga aatgctgggt gaaggtataa
     5461 tgacatgtga cgtcccctcc agggatgtag aacttctcca cactgttaaa gatgacctga
     5521 gagaaatgac agtgatccag caagacagct gatgtggggg gatctttgat ggtctcctcc
     5581 atgccaactt ttttgtacaa acttgtttga taacctcgag cggcatcagc aaacccaagc
     5641 tcggaaagct tttggtgagc ctcagcgtaa tcagcaaaga aggcatcttc gtccgctgca
     5701 tatttgtcaa cgagagggcg gaatacaggg tcagacaaaa gagccttgtc agaaggtagc
     5761 tgaaggagac cttccttctc accactcaac aactccgtga agtatgagtt gtcgaaaata
     5821 agaggattag aggtccaggg accctcaaat ccagaacgct ccttgtgtgc agctccaata
     5881 gtgtgacccc cagatagagc aacgatatct tggtcagtaa gccccatagc tttgccaaac
     5941 acatctctca aatggtcaga acccttagtg ggatcgggca agcgaccctc tggtggtggc
     6001 tcaggcttgt cctctcttcc agggtggaat ggaaccttag gtccacccgt gacctcaacg
     6061 gcaacaacgc cagccaactg gtagaaatcg gcgtagctca aaatagggaa ctccgccttg
     6121 agtggctcca aaagcctaac agcgatgtca agaccgttgt tagcgctgtg agccagttcg
     6181 gcagggtgct tgatggttcc gaagggtcca ccggtcttcg tgcccttgtc aaaggttcca
     6241 gcagagtgga atgccaaacg gagcattaga ggagcgcatc tcttctcagc gatgaagcct
     6301 ctgagcttct tcttcgcctt ctcaacggcg tcctggtaat cagcactcac agttgggtaa
     6361 gactttccct tatcgtcgtc atccttgtag tccatcatgg tggcagatca acctcgagag
     6421 atctgcggcc gccggtgtct tctatggagg tcaaaacagc gtggatggcg tctccaggcg
     6481 atctgacggt tcactaaacg agctctgctt atatagacct cccaccgtac acgcctaccg
     6541 cccatttgcg tcaatggggc ggagttgtta cgacattttg gaaagtcccg ttgattttgg
     6601 tgccaaaaca aactcccatt gacgtcaatg gggtggagac ttggaaatcc ccgtgagtca
     6661 aaccgctatc cacgcccatt gatgtactgc caaaaccgca tcaccatggt aatagcgatg
     6721 actaatacgt agatgtactg ccaagtagga aagtcccata aggtcatgta ctgggcataa
     6781 tgccaggcgg gccatttacc gtcattgacg tcaatagggg gcgtacttgg catatgatac
     6841 acttgatgta ctgccaagtg ggcagtttac cgtaaatact ccacccattg acgtcaatgg
     6901 aaagtcccta ttggcgttac tatgggaaca tacgtcatta ttgacgtcaa tgggcggggg
     6961 tcgttgggcg gtcagccagg cgggccattt accgtaagtt atgtaacgcg gaactccact
     7021 agtccaaact ggatctctgc tgtccctgta ataaacccga aaattttgaa tttttgtaat
     7081 ttgtttttgt aattctttag tttgtatgtc tgttgctatt atgtctacta ttctttcccc
     7141 tgcactgtac cccccaatcc ccccttttct tttaaaattg tggatgaata ctgccatttg
     7201 tgaattaatt cggcgatacc gtcgagatcc gttcactaat cgaatggatc tgtctctgtc
     7261 tctctctcca ccttcttctt ctattccttc gggcctgtcg ggtcccctcg gggttgggag
     7321 gtgggtctga aacgataatg gtgaatatcc ctgcctaact ctattcacta tagaaagtac
     7381 agcaaaaact attcttaaac ctaccaagcc tcctactatc attatgaata attttatata
     7441 ccacagccaa tttgttatgt taaaccaatt ccacaaactt gcccatttat ctaattccaa
     7501 taattcttgt tcattctttt cttgctggtt ttgcgattct tcaattaagg agtgtattaa
     7561 gcttgtgtaa ttgttaattt ctctgtccca ctccatccag gtcgtgtgat tccaaatctg
     7621 ttccagagat ttattactcc aactagcatt ccaaggcaca gcagtggtgc aaatgagttt
     7681 tccagagcaa ccccaaatcc ccaggagctg ttgatccttt aggtatcttt ccacagccag
     7741 gattcttgcc tggagctgct tgatgcccca gactgtgagt tgcaacagat gctgttgcgc
     7801 ctcaatagcc ctcagcaaat tgttctgctg ctgcactata ccagacaata attgtctggc
     7861 ctgtaccgtc agcgtcattg acgctgcgcc catagtgctt cctgctgctc ccaagaaccc
     7921 aaggaacaaa gctcctattc ccactgctct tttttctctc tgcaccactc ttctctttgc
     7981 cttggtgggt gctactccta atggttcaat ttttactact ttatatttat ataattcact
     8041 tctccaattg tccctcatat ctcctcctcc aggtctgaag atcagcggcc ggccgcttgc
     8101 tgtgcggtgg tcttactttt gttttgctct tcctctatct tgtctaaagc ttccttggtg
     8161 tcttttatct ctatcctttg atgcacacaa tagagggttg ctactgtatt atataatgat
     8221 ctaagttctt ctgatcctgt ctgaagggat ggttgtagct gtcccagtat ttgtctacag
     8281 ccttctgatg tttctaacag gccaggatta actgcgaatc gttctagctc cctgcttgcc
     8341 catactatat gttttaattt atattttttc tttccccctg gccttaaccg aattttttcc
     8401 catcgcgatc taattctccc ccgcttaata ctgacgctct cgcacccatc tctctccttc
     8461 tagcctccgc tagtcaaaat ttttggcgta ctcaccagtc gccgcccctc gcctcttgcc
     8521 gtgcgcgctt cagcaagccg agtcctgcgt cgagagagct cctctggttt ccctttcgct
     8581 ttcaagtccc tgttcgggcg ccactgctag agattttcca cactgactaa aagggtctga
     8641 gggatctcta gttaccagag tcacacaaca gacgggcaca cactacttga agcactcaag
     8701 gcaagcttta ttgaggctta agcagtgggt tccctagtta gccagagagc tcccaggctc
     8761 agatctggtc taaccagaga gacccagtac aggcaaaaag cagctgctta tatgcaggat
     8821 ctgagggctc gccactcccc agtcccgccc aggccacgcc tccctggaaa gtccccagcg
     8881 gaaagtccct tgtagcaagc tcgatatcag cagttcttga agtactccgg atgcagctct
     8941 cgggccacgt gatgaaatgc taggcggctg tcaaacctcc actctaacac ttctctctcc
     9001 gggtcatcca tcccatgcag gctcacaggg tgtaacaagc tggtgttctc tcctttattg
     9061 gcctcttcta ccttatctgg ctcaactggt actagcttgt agcaccatcc aaaggtcagt
     9121 ggatatctga cccctggccc tggtgtgtag ttctgctaat cagggaagta gccttgtgtg
     9181 tggtagatcc acagatcaag gatatcttgt cttctttggg agtgaattag cccttccaac
     9241 tactaagttt gtagtacata tttaacaaat acaatttctt taaaatgaaa ataattcaga
     9301 ggaatcacag gtttagagta aatgaaacca caggtaattg gcagtggtaa tagggtatgg
     9361 ggtgggaagt ttgggatgat tttggttagc ttgagttatc cagttgatcc agacatgata
     9421 agatacattg atgagtttgg acaaaccaca actagaatgc agtgaaaaaa atgctttatt
     9481 tgtgaaattc gtaatcatgt catagctgtt tcctgtgtga aattgttatc cgctcacaat
     9541 tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag
     9601 ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg
     9661 ccacggggag ctttttgcaa aagcctaggc ctccaaaaaa gcctcctcac tacttctgga
     9721 atagctcaga ggccgaggcg gcctcggcct ctgcataaat aaaaaaaatt agtcagccat
     9781 gagcttgctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg
//
