LOCUS       sequence-346593-       10233 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector expressing N-terminally tagged APEX2-FLAG-OPTN.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10233)
  AUTHORS   Eapen VV, Swarup S, Hoyer MJ, Paulo JA, Harper JW
  TITLE     Quantitative proteomics reveals the selectivity of ubiquitin-binding
            autophagy receptors in the turnover of damaged lysosomes by 
            lysophagy.
  JOURNAL   eLife. 2021 Sep 29;10. pii: 72328. doi: 10.7554/eLife.72328.
  PUBMED    34585663
REFERENCE   2  (bases 1 to 10233)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "eLife."; 
            date: "2021-09-29"; volume: "10. pii: 72328. doi"; pages: " 
            10.7554/eLife.72328"
COMMENT     Sequence Label: pHAGE-FLAG-APEX2-OPTN
FEATURES             Location/Qualifiers
     source          1..10233
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2062..2179
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        2231..2534
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2535..2738
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2689..2709
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2735..2759
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             2847..2870
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             2871..3617
                     /codon_start=1
                     /product="soybean ascorbate peroxidase, engineered to serve
                     as a monomeric tag for electron microscopy and live-cell 
                     proteomics (Lam et al., 2015)"
                     /label=APEX2
                     /note="more active than the original APEX due to the A134P 
                     mutation"
                     /translation="GKSYPTVSADYQDAVEKAKKKLRGFIAEKRCAPLMLRLAFHSAGT
                     FDKGTKTGGPFGTIKHPAELAHSANNGLDIAVRLLEPLKAEFPILSYADFYQLAGVVAV
                     EVTGGPKVPFHPGREDKPEPPPEGRLPDPTKGSDHLRDVFGKAMGLTDQDIVALSGGHT
                     IGAAHKERSGFEGPWTSNPLIFDNSYFTELLSGEKEGLLQLPSDKALLSDPVFRPLVDK
                     YAADEDAFFADYAEAHQKLSELGFADA"
     protein_bind    3633..3657
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             3974..3985
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    complement(5389..5413)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    5426..5993
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    5443..5995
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5587..5604)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5814..5833
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             6007..6606
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6007..6026)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6503..6523
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    6623..7211
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6676..6696)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7094..7105)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7286..7519
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(7638..7656)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7724..7828
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7829..7897,7898..8689)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8047..8066)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8860..9448
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9349..9368
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9602..9619
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9859..9880
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9895..9900,9901..9918,9919..9925)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9933..9949
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9938..9960
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9957..9973
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9957..9973
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     10023..10042
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttaattcaca
     2041 aatggcagta ttcatccaca attttaaaag aaaagggggg attggggggt acagtgcagg
     2101 ggaaagaata gtagacataa tagcaacaga catacaaact aaagaattac aaaaacaaat
     2161 tacaaaaatt caaaattttc gggtttatta cagggacagc agagatccag tttggactag
     2221 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
     2281 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
     2341 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
     2401 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
     2461 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
     2521 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
     2581 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
     2641 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
     2701 gtaggcgtgt acggtgggag gtctatataa gcagagctcg tttagtgaac cgtcagatcg
     2761 cctggagacg ccatccacgc tgttttgacc tccatagaag acaccggcgg ccgcagatct
     2821 ctcgaggttg atctgccacc atgatggact acaaggatga cgacgataag ggaaagtctt
     2881 acccaactgt gagtgctgat taccaggacg ccgttgagaa ggcgaagaag aagctcagag
     2941 gcttcatcgc tgagaagaga tgcgctcctc taatgctccg tttggcattc cactctgctg
     3001 gaacctttga caagggcacg aagaccggtg gacccttcgg aaccatcaag caccctgccg
     3061 aactggctca cagcgctaac aacggtcttg acatcgctgt taggcttttg gagccactca
     3121 aggcggagtt ccctattttg agctacgccg atttctacca gttggctggc gttgttgccg
     3181 ttgaggtcac gggtggacct aaggttccat tccaccctgg aagagaggac aagcctgagc
     3241 caccaccaga gggtcgcttg cccgatccca ctaagggttc tgaccatttg agagatgtgt
     3301 ttggcaaagc tatggggctt actgaccaag atatcgttgc tctatctggg ggtcacacta
     3361 ttggagctgc acacaaggag cgttctggat ttgagggtcc ctggacctct aatcctctta
     3421 ttttcgacaa ctcatacttc acggagttgt tgagtggtga gaaggaaggt ctccttcagc
     3481 taccttctga caaggctctt ttgtctgacc ctgtattccg ccctctcgtt gacaaatatg
     3541 cagcggacga agatgccttc tttgctgatt acgctgaggc tcaccaaaag ctttccgagc
     3601 ttgggtttgc tgatgccgct cgaggttatc aaacaagttt gtacaaaaaa gttggcatgt
     3661 cccatcaacc tctcagctgc ctcactgaaa aggaggacag ccccagtgaa agcacaggaa
     3721 atggaccccc ccacctggcc cacccaaacc tggacacgtt taccccggag gagctgctgc
     3781 agcagatgaa agagctcctg accgagaacc accagctgaa agaagccatg aagctaaata
     3841 atcaagccat gaaagggaga tttgaggagc tttcggcctg gacagagaaa cagaaggaag
     3901 aacgccagtt ttttgagata cagagcaaag aagcaaaaga gcgtctaatg gccttgagtc
     3961 atgagaatga gaaattgaag gaagagcttg gaaaactaaa agggaaatca gaaaggtcat
     4021 ctgaggaccc cactgatgac tccaggcttc ccagggccga agcggagcag gaaaaggacc
     4081 agctcaggac ccaggtggtg aggctacaag cagagaaggc agacctgttg ggcatcgtgt
     4141 ctgaactgca gctcaagctg aactccagcg gctcctcaga agattccttt gttgaaatta
     4201 ggatggctga aggagaagca gaagggtcag taaaagaaat caagcatagt cctgggccca
     4261 cgagaacagt ctccactggc acggcattgt ctaaatatag gagcagatct gcagatgggg
     4321 ccaagaatta cttcgaacat gaggagttaa ctgtgagcca gctcctgctg tgcctaaggg
     4381 aagggaatca gaaggtggag agacttgaag ttgcactcaa ggaggccaaa gaaagagttt
     4441 cagattttga aaagaaaaca agtaatcgtt ctgagattga aacccagaca gaggggagca
     4501 cagagaaaga gaatgatgaa gagaaaggcc cggagactgt tggaagcgaa gtggaagcac
     4561 tgaacctcca ggtgacatct ctgtttaagg agcttcaaga ggctcataca aaactcagcg
     4621 aagctgagct aatgaagaag agacttcaag aaaagtgtca ggcccttgaa aggaaaaatt
     4681 ctgcaattcc atcagagttg aatgaaaagc aagagcttgt ttatactaac aaaaagttag
     4741 agctacaagt ggaaagcatg ctatcagaaa tcaaaatgga acaggctaaa acagaggatg
     4801 aaaagtccaa attaactgtg ctacagatga cacacaacaa gcttcttcaa gaacataata
     4861 atgcattgaa aacaattgag gaactaacaa gaaaagagtc agaaaaagtg gacagggcag
     4921 tgctgaagga actgagtgaa aaactggaac tggcagagaa ggctctggct tccaaacagc
     4981 tgcaaatgga tgaaatgaag caaaccattg ccaagcagga agaggacctg gaaaccatga
     5041 ccatcctcag ggctcagatg gaagtttact gttctgattt tcatgctgaa agagcagcga
     5101 gagagaaaat tcatgaggaa aaggagcaac tggcattgca gctggcagtt ctgctgaaag
     5161 agaatgatgc tttcgaagac ggaggcaggc agtccttgat ggagatgcag agtcgtcatg
     5221 gggcgagaac aagtgactct gaccagcagg cttaccttgt tcaaagagga gctgaggaca
     5281 gggactggcg gcaacagcgg aatattccga ttcattcctg ccccaagtgt ggagaggttc
     5341 tgcctgacat agacacgtta cagattcacg tgatggattg catcatttac ccaactttct
     5401 tgtacaaagt ggttcgataa cgaattccgc cccccccccc taacgttact ggccgaagcc
     5461 gcttggaata aggccggtgt gcgtttgtct atatgttatt ttccaccata ttgccgtctt
     5521 ttggcaatgt gagggcccgg aaacctggcc ctgtcttctt gacgagcatt cctaggggtc
     5581 tttcccctct cgccaaagga atgcaaggtc tgttgaatgt cgtgaaggaa gcagttcctc
     5641 tggaagcttc ttgaagacaa acaacgtctg tagcgaccct ttgcaggcag cggaaccccc
     5701 cacctggcga caggtgcctc tgcggccaaa agccacgtgt ataagataca cctgcaaagg
     5761 cggcacaacc ccagtgccac gttgtgagtt ggatagttgt ggaaagagtc aaatggctct
     5821 cctcaagcgt attcaacaag gggctgaagg atgcccagaa ggtaccccat tgtatgggat
     5881 ctgatctggg gcctcggtgc acatgcttta catgtgttta gtcgaggtta aaaaaacgtc
     5941 taggcccccc gaaccacggg gacgtggttt tcctttgaaa aacacgatga taatatggcc
     6001 acaaccatga ccgagtacaa gcccacggtg cgcctcgcca cccgcgacga cgtccccagg
     6061 gccgtacgca ccctcgccgc cgcgttcgcc gactaccccg ccacgcgcca caccgtcgat
     6121 ccggaccgcc acatcgagcg ggtcaccgag ctgcaagaac tcttcctcac gcgcgtcggg
     6181 ctcgacatcg gcaaggtgtg ggtcgcggac gacggcgccg cggtggcggt ctggaccacg
     6241 ccggagagcg tcgaagcggg ggcggtgttc gccgagatcg gcccgcgcat ggccgagttg
     6301 agcggttccc ggctggccgc gcagcaacag atggaaggcc tcctggcgcc gcaccggccc
     6361 aaggagcccg cgtggttcct ggccaccgtc ggcgtctcgc ccgaccacca gggcaagggt
     6421 ctgggcagcg ccgtcgtgct ccccggagtg gaggcggccg agcgcgccgg ggtgcccgcc
     6481 ttcctggaga cctccgcgcc ccgcaacctc cccttctacg agcggctcgg cttcaccgtc
     6541 accgccgacg tcgaggtgcc cgaaggaccg cgcacctggt gcatgacccg caagcccggt
     6601 gcctgatgca tcgatggatc ctaatcaacc tctggattac aaaatttgtg aaagattgac
     6661 tggtattctt aactatgttg ctccttttac gctatgtgga tacgctgctt taatgccttt
     6721 gtatcatgct attgcttccc gtatggcttt cattttctcc tccttgtata aatcctggtt
     6781 gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt
     6841 gtttgctgac gcaaccccca ctggttgggg cattgccacc acctgtcagc tcctttccgg
     6901 gactttcgct ttccccctcc ctattgccac ggcggaactc atcgccgcct gccttgcccg
     6961 ctgctggaca ggggctcggc tgttgggcac tgacaattcc gtggtgttgt cggggaaatc
     7021 atcgtccttt ccttggctgc tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt
     7081 ctgctacgtc ccttcggccc tcaatccagc ggaccttcct tcccgcggcc tgctgccggc
     7141 tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg agtcggatct ccctttgggc
     7201 cgcctccccg cctgagatcc tttaagacca atgacttaca aggcagctgt agatcttagc
     7261 cactttttaa aagaaaaggg gggactggaa gggctaattc actcccaacg aagacaagat
     7321 ctgctttttg cttgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct
     7381 ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta
     7441 gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca
     7501 gtgtggaaaa tctctagcag tagtagttca tgtcatctta ttattcagta tttataactt
     7561 gcaaagaaat gaatatcaga gagtgagagg cccgggttaa ttaaggaaag ggctagatca
     7621 ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat
     7681 aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg
     7741 tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat
     7801 gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat
     7861 tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt
     7921 aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag
     7981 cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa
     8041 agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg
     8101 ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct
     8161 tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac
     8221 tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca
     8281 caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat
     8341 accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact
     8401 attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc
     8461 ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga
     8521 taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg
     8581 taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg
     8641 aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca
     8701 agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta
     8761 ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca
     8821 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
     8881 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
     8941 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
     9001 tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
     9061 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
     9121 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
     9181 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
     9241 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
     9301 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
     9361 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
     9421 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct
     9481 ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga
     9541 taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg
     9601 cagcgagtca gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc
     9661 gcgttggccg attcattaat gcagcaagct catggctgac taattttttt tatttatgca
     9721 gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga
     9781 ggcctaggct tttgcaaaaa gctccccgtg gcacgacagg tttcccgact ggaaagcggg
     9841 cagtgagcgc aacgcaatta atgtgagtta gctcactcat taggcacccc aggctttaca
     9901 ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc ggataacaat ttcacacagg
     9961 aaacagctat gacatgatta cgaatttcac aaataaagca tttttttcac tgcattctag
    10021 ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggatcaact ggataactca
    10081 agctaaccaa aatcatccca aacttcccac cccataccct attaccactg ccaattacct
    10141 gtggtttcat ttactctaaa cctgtgattc ctctgaatta ttttcatttt aaagaaattg
    10201 tatttgttaa atatgtacta caaacttagt agt
//
