LOCUS       sequence-346594-       10869 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector expressing N-terminally tagged APEX2-FLAG-TAX1BP1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10869)
  AUTHORS   Eapen VV, Swarup S, Hoyer MJ, Paulo JA, Harper JW
  TITLE     Quantitative proteomics reveals the selectivity of ubiquitin-binding
            autophagy receptors in the turnover of damaged lysosomes by 
            lysophagy.
  JOURNAL   eLife. 2021 Sep 29;10. pii: 72328. doi: 10.7554/eLife.72328.
  PUBMED    34585663
REFERENCE   2  (bases 1 to 10869)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "eLife."; 
            date: "2021-09-29"; volume: "10. pii: 72328. doi"; pages: " 
            10.7554/eLife.72328"
COMMENT     Sequence Label: pHAGE-FLAG-APEX2-TAX1BP1
FEATURES             Location/Qualifiers
     source          1..10869
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2062..2179
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        2231..2534
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2535..2738
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2689..2709
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2735..2759
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             2847..2870
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             2871..3617
                     /codon_start=1
                     /product="soybean ascorbate peroxidase, engineered to serve
                     as a monomeric tag for electron microscopy and live-cell 
                     proteomics (Lam et al., 2015)"
                     /label=APEX2
                     /note="more active than the original APEX due to the A134P 
                     mutation"
                     /translation="GKSYPTVSADYQDAVEKAKKKLRGFIAEKRCAPLMLRLAFHSAGT
                     FDKGTKTGGPFGTIKHPAELAHSANNGLDIAVRLLEPLKAEFPILSYADFYQLAGVVAV
                     EVTGGPKVPFHPGREDKPEPPPEGRLPDPTKGSDHLRDVFGKAMGLTDQDIVALSGGHT
                     IGAAHKERSGFEGPWTSNPLIFDNSYFTELLSGEKEGLLQLPSDKALLSDPVFRPLVDK
                     YAADEDAFFADYAEAHQKLSELGFADA"
     protein_bind    3633..3657
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(6025..6049)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    6062..6629
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    6079..6631
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(6223..6240)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     6450..6469
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             6643..7242
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6643..6662)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     7139..7159
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    7259..7847
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7312..7332)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7730..7741)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7922..8155
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(8274..8292)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8360..8464
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8465..8533,8534..9325)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8683..8702)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9496..10084
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9985..10004
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10238..10255
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    10495..10516
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(10531..10536,10537..10554,10555..10561)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10569..10585
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10574..10596
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     10593..10609
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10593..10609
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     10659..10678
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttaattcaca
     2041 aatggcagta ttcatccaca attttaaaag aaaagggggg attggggggt acagtgcagg
     2101 ggaaagaata gtagacataa tagcaacaga catacaaact aaagaattac aaaaacaaat
     2161 tacaaaaatt caaaattttc gggtttatta cagggacagc agagatccag tttggactag
     2221 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
     2281 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
     2341 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
     2401 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
     2461 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
     2521 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
     2581 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
     2641 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
     2701 gtaggcgtgt acggtgggag gtctatataa gcagagctcg tttagtgaac cgtcagatcg
     2761 cctggagacg ccatccacgc tgttttgacc tccatagaag acaccggcgg ccgcagatct
     2821 ctcgaggttg atctgccacc atgatggact acaaggatga cgacgataag ggaaagtctt
     2881 acccaactgt gagtgctgat taccaggacg ccgttgagaa ggcgaagaag aagctcagag
     2941 gcttcatcgc tgagaagaga tgcgctcctc taatgctccg tttggcattc cactctgctg
     3001 gaacctttga caagggcacg aagaccggtg gacccttcgg aaccatcaag caccctgccg
     3061 aactggctca cagcgctaac aacggtcttg acatcgctgt taggcttttg gagccactca
     3121 aggcggagtt ccctattttg agctacgccg atttctacca gttggctggc gttgttgccg
     3181 ttgaggtcac gggtggacct aaggttccat tccaccctgg aagagaggac aagcctgagc
     3241 caccaccaga gggtcgcttg cccgatccca ctaagggttc tgaccatttg agagatgtgt
     3301 ttggcaaagc tatggggctt actgaccaag atatcgttgc tctatctggg ggtcacacta
     3361 ttggagctgc acacaaggag cgttctggat ttgagggtcc ctggacctct aatcctctta
     3421 ttttcgacaa ctcatacttc acggagttgt tgagtggtga gaaggaaggt ctccttcagc
     3481 taccttctga caaggctctt ttgtctgacc ctgtattccg ccctctcgtt gacaaatatg
     3541 cagcggacga agatgccttc tttgctgatt acgctgaggc tcaccaaaag ctttccgagc
     3601 ttgggtttgc tgatgccgct cgaggttatc aaacaagttt gtacaaaaaa gttggcatga
     3661 catcctttca agaagtccca ttgcagactt ccaactttgc ccatgtcatc tttcaaaatg
     3721 tggccaagag ttaccttcct aatgcacacc tggaatgtca ttacacctta actccatata
     3781 ttcatccaca tccaaaagat tgggttggta tattcaaggt tggatggagt actgctcgtg
     3841 attattacac gtttttatgg tcccctatgc ctgaacatta tgtggaagga tcaacagtca
     3901 attgtgtact agcattccaa ggatattacc ttccaaatga tgatggagaa ttttatcagt
     3961 tctgttacgt tacccataag ggtgaaattc gtggagcaag tacacctttc cagtttcgag
     4021 cttcttctcc agttgaagag ctgcttacta tggaagatga aggaaattct gacatgttag
     4081 tggtgaccac aaaagcaggc cttcttgagt tgaaaattga gaaaaccatg aaagaaaaag
     4141 aagaactgtt aaagttaatt gccgttctgg aaaaagaaac agcacaactt cgagaacaag
     4201 ttgggagaat ggaaagagaa cttaaccatg agaaagaaag atgtgaccaa ctgcaagcag
     4261 aacaaaaggg tcttactgaa gtaacacaaa gcttaaaaat ggaaaatgaa gagtttaaga
     4321 agaggttcag tgatgctaca tccaaagccc atcagcttga ggaagatatt gtgtcagtaa
     4381 cacataaagc aattgaaaaa gaaaccgaat tagacagttt aaaggacaaa ctcaagaagg
     4441 cacaacatga aagagaacaa cttgaatgtc agttgaagac agagaaggat gaaaaggaac
     4501 tttataaggt acatttgaag aatacagaaa tagaaaatac caagcttatg tcagaggtcc
     4561 agactttaaa aaatttagat gggaacaaag aaagcgtgat tactcatttc aaagaagaga
     4621 ttggcaggct gcagttatgt ttggctgaaa aggaaaatct gcaaagaact ttcctgctta
     4681 caacctcaag taaagaagat acttgttttt taaaggagca acttcgtaaa gcagaggaac
     4741 aggttcaggc aactcggcaa gaagttgtct ttctggctaa agaactcagt gatgctgtca
     4801 acgtacgaga cagaacgatg gcagacctgc atactgcacg cttggaaaac gagaaagtga
     4861 aaaagcagtt agctgatgca gtggcagaac ttaaactaaa tgctatgaaa aaagatcagg
     4921 acaagactga tacactggaa cacgaactaa gaagagaagt tgaagatctg aaactccgtc
     4981 ttcagatggc tgcagaccat tataaagaaa aatttaagga atgccaaagg ctccaaaaac
     5041 aaataaacaa actttcagat caatcagcta ataataataa tgtcttcaca aagaaaacgg
     5101 ggaatcagca gaaagtgaat gatgcttcag taaacacaga cccagccact tctgcctcta
     5161 ctgtagatgt aaagccatca ccttctgcag cagaggcaga ttttgacata gtaacaaagg
     5221 ggcaagtctg tgaaatgacc aaagaaattg ctgacaaaac agaaaagtat aataaatgta
     5281 aacaactctt gcaggatgag aaagcaaaat gcaataaata tgctgatgaa cttgcaaaaa
     5341 tggagctgaa atggaaagaa caagtgaaaa ttgctgaaaa tgtaaaactt gaactagctg
     5401 aagtacagga caattataaa gaacttaaaa ggagtctaga aaatccagca gaaaggaaaa
     5461 tggaaggtca gaattcccag agtcctcaat gtttcaaaac atgctcagag caaaatggtt
     5521 atgttctcac attgtcaaat gcacaaccag ttctgcaata tggtaatcct tatgcatctc
     5581 aggaaacaag agatggagca gatggtgctt tttacccaga tgaaatacaa aggccacctg
     5641 tcagagtccc ctcttgggga ctggaagaca atgttgtctg cagccagcct gctcgaaact
     5701 ttagtcggcc tgatggctta gaggactctg aggatagcaa agaagatgag aatgtgccta
     5761 ctgctcctga tcctccaagt caacatttac gtgggcatgg gacaggcttt tgctttgatt
     5821 ccagctttga tgttcacaag aagtgtcccc tctgtgagtt aatgtttcct cctaactatg
     5881 atcagagcaa atttgaagaa catgttgaaa gtcactggaa ggtgtgcccg atgtgcagcg
     5941 agcagttccc tcctgactat gaccagcagg tgtttgaaag gcatgtgcag acccattttg
     6001 atcagaatgt tctaaatttt gactacccaa ctttcttgta caaagtggtt cgataacgaa
     6061 ttccgccccc cccccctaac gttactggcc gaagccgctt ggaataaggc cggtgtgcgt
     6121 ttgtctatat gttattttcc accatattgc cgtcttttgg caatgtgagg gcccggaaac
     6181 ctggccctgt cttcttgacg agcattccta ggggtctttc ccctctcgcc aaaggaatgc
     6241 aaggtctgtt gaatgtcgtg aaggaagcag ttcctctgga agcttcttga agacaaacaa
     6301 cgtctgtagc gaccctttgc aggcagcgga accccccacc tggcgacagg tgcctctgcg
     6361 gccaaaagcc acgtgtataa gatacacctg caaaggcggc acaaccccag tgccacgttg
     6421 tgagttggat agttgtggaa agagtcaaat ggctctcctc aagcgtattc aacaaggggc
     6481 tgaaggatgc ccagaaggta ccccattgta tgggatctga tctggggcct cggtgcacat
     6541 gctttacatg tgtttagtcg aggttaaaaa aacgtctagg ccccccgaac cacggggacg
     6601 tggttttcct ttgaaaaaca cgatgataat atggccacaa ccatgaccga gtacaagccc
     6661 acggtgcgcc tcgccacccg cgacgacgtc cccagggccg tacgcaccct cgccgccgcg
     6721 ttcgccgact accccgccac gcgccacacc gtcgatccgg accgccacat cgagcgggtc
     6781 accgagctgc aagaactctt cctcacgcgc gtcgggctcg acatcggcaa ggtgtgggtc
     6841 gcggacgacg gcgccgcggt ggcggtctgg accacgccgg agagcgtcga agcgggggcg
     6901 gtgttcgccg agatcggccc gcgcatggcc gagttgagcg gttcccggct ggccgcgcag
     6961 caacagatgg aaggcctcct ggcgccgcac cggcccaagg agcccgcgtg gttcctggcc
     7021 accgtcggcg tctcgcccga ccaccagggc aagggtctgg gcagcgccgt cgtgctcccc
     7081 ggagtggagg cggccgagcg cgccggggtg cccgccttcc tggagacctc cgcgccccgc
     7141 aacctcccct tctacgagcg gctcggcttc accgtcaccg ccgacgtcga ggtgcccgaa
     7201 ggaccgcgca cctggtgcat gacccgcaag cccggtgcct gatgcatcga tggatcctaa
     7261 tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc
     7321 ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat
     7381 ggctttcatt ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg
     7441 gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg
     7501 ttggggcatt gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat
     7561 tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt
     7621 gggcactgac aattccgtgg tgttgtcggg gaaatcatcg tcctttcctt ggctgctcgc
     7681 ctgtgttgcc acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa
     7741 tccagcggac cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtcttcg
     7801 ccttcgccct cagacgagtc ggatctccct ttgggccgcc tccccgcctg agatccttta
     7861 agaccaatga cttacaaggc agctgtagat cttagccact ttttaaaaga aaagggggga
     7921 ctggaagggc taattcactc ccaacgaaga caagatctgc tttttgcttg tactgggtct
     7981 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
     8041 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
     8101 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtagt
     8161 agttcatgtc atcttattat tcagtattta taacttgcaa agaaatgaat atcagagagt
     8221 gagaggcccg ggttaattaa ggaaagggct agatcattct tgaagacgaa agggcctcgt
     8281 gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg
     8341 cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa
     8401 tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa
     8461 gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct
     8521 tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg
     8581 tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg
     8641 ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt
     8701 atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga
     8761 cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga
     8821 attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac
     8881 gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg
     8941 ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac
     9001 gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct
     9061 agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct
     9121 gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg
     9181 gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat
     9241 ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg
     9301 tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat
     9361 tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct
     9421 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa
     9481 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
     9541 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
     9601 gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta
     9661 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
     9721 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
     9781 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
     9841 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc
     9901 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
     9961 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
    10021 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
    10081 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca
    10141 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg
    10201 agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc
    10261 ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag
    10321 caagctcatg gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct
    10381 gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg caaaaagctc
    10441 cccgtggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt
    10501 gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg ctcgtatgtt
    10561 gtgtggaatt gtgagcggat aacaatttca cacaggaaac agctatgaca tgattacgaa
    10621 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa
    10681 tgtatcttat catgtctgga tcaactggat aactcaagct aaccaaaatc atcccaaact
    10741 tcccacccca taccctatta ccactgccaa ttacctgtgg tttcatttac tctaaacctg
    10801 tgattcctct gaattatttt cattttaaag aaattgtatt tgttaaatat gtactacaaa
    10861 cttagtagt
//
