LOCUS       sequence-346596-       10071 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector expressing N-terminally tagged eGFP-OPTN D474N.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10071)
  AUTHORS   Eapen VV, Swarup S, Hoyer MJ, Paulo JA, Harper JW
  TITLE     Quantitative proteomics reveals the selectivity of ubiquitin-binding
            autophagy receptors in the turnover of damaged lysosomes by 
            lysophagy.
  JOURNAL   eLife. 2021 Sep 29;10. pii: 72328. doi: 10.7554/eLife.72328.
  PUBMED    34585663
REFERENCE   2  (bases 1 to 10071)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "eLife."; 
            date: "2021-09-29"; volume: "10. pii: 72328. doi"; pages: " 
            10.7554/eLife.72328"
COMMENT     Sequence Label: pHAGE-eGFP-OPTN D474N
FEATURES             Location/Qualifiers
     source          1..10071
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2058..2175
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        2227..2530
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2531..2734
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2685..2705
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2731..2755
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      2814..2823
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(2820..2822,2823..2825,2826..3536)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(2820..2822,2823..2825,2826..3536)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2865..2886)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3126..3145)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3473..3494
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    3552..3576
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             3893..3904
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    complement(5308..5332)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        5351..5850
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(5386..5409)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     5783..5802
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             5874..6272
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     polyA_signal    6314..6435
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6351..6370)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     misc_feature    6461..7049
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6514..6534)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6932..6943)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7124..7357
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(7476..7494)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7562..7666
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7667..7735,7736..8527)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7885..7904)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8698..9286
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9187..9206
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9440..9457
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9697..9718
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9733..9738,9739..9756,9757..9763)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9771..9787
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9776..9798
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9795..9811
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9795..9811
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg
     2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa
     2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca
     2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactagtgga
     2221 gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg
     2281 cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg
     2341 acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca
     2401 tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc
     2461 ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc
     2521 tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc
     2581 acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa
     2641 tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag
     2701 gcgtgtacgg tgggaggtct atataagcag agctcgttta gtgaaccgtc agatcgcctg
     2761 gagacgccat ccacgctgtt ttgacctcca tagaagacac cggcggccga tctgccacca
     2821 tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc catcctggtc gagctggacg
     2881 gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg
     2941 gcaagctgac cctgaagttc atctgcacca ccggcaagct gcccgtgccc tggcccaccc
     3001 tcgtgaccac cctgacctac ggcgtgcagt gcttcagccg ctaccccgac cacatgaagc
     3061 agcacgactt cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc accatcttct
     3121 tcaaggacga cggcaactac aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg
     3181 tgaaccgcat cgagctgaag ggcatcgact tcaaggagga cggcaacatc ctggggcaca
     3241 agctggagta caactacaac agccacaacg tctatatcat ggccgacaag cagaagaacg
     3301 gcatcaaggt gaacttcaag atccgccaca acatcgagga cggcagcgtg cagctcgccg
     3361 accactacca gcagaacacc cccatcggcg acggccccgt gctgctgccc gacaaccact
     3421 acctgagcac ccagtccgcc ctgagcaaag accccaacga gaagcgcgat cacatggtcc
     3481 tgctggagtt cgtgaccgcc gccgggatca ctctcggcat ggacgagctg tacaaggctc
     3541 gaggttatca aacaagtttg tacaaaaaag ttggcatgtc ccatcaacct ctcagctgcc
     3601 tcactgaaaa ggaggacagc cccagtgaaa gcacaggaaa tggacccccc cacctggccc
     3661 acccaaacct ggacacgttt accccggagg agctgctgca gcagatgaaa gagctcctga
     3721 ccgagaacca ccagctgaaa gaagccatga agctaaataa tcaagccatg aaagggagat
     3781 ttgaggagct ttcggcctgg acagagaaac agaaggaaga acgccagttt tttgagatac
     3841 agagcaaaga agcaaaagag cgtctaatgg ccttgagtca tgagaatgag aaattgaagg
     3901 aagagcttgg aaaactaaaa gggaaatcag aaaggtcatc tgaggacccc actgatgact
     3961 ccaggcttcc cagggccgaa gcggagcagg aaaaggacca gctcaggacc caggtggtga
     4021 ggctacaagc agagaaggca gacctgttgg gcatcgtgtc tgaactgcag ctcaagctga
     4081 actccagcgg ctcctcagaa gattcctttg ttgaaattag gatggctgaa ggagaagcag
     4141 aagggtcagt aaaagaaatc aagcatagtc ctgggcccac gagaacagtc tccactggca
     4201 cggcattgtc taaatatagg agcagatctg cagatggggc caagaattac ttcgaacatg
     4261 aggagttaac tgtgagccag ctcctgctgt gcctaaggga agggaatcag aaggtggaga
     4321 gacttgaagt tgcactcaag gaggccaaag aaagagtttc agattttgaa aagaaaacaa
     4381 gtaatcgttc tgagattgaa acccagacag aggggagcac agagaaagag aatgatgaag
     4441 agaaaggccc ggagactgtt ggaagcgaag tggaagcact gaacctccag gtgacatctc
     4501 tgtttaagga gcttcaagag gctcatacaa aactcagcga agctgagcta atgaagaaga
     4561 gacttcaaga aaagtgtcag gcccttgaaa ggaaaaattc tgcaattcca tcagagttga
     4621 atgaaaagca agagcttgtt tatactaaca aaaagttaga gctacaagtg gaaagcatgc
     4681 tatcagaaat caaaatggaa caggctaaaa cagaggatga aaagtccaaa ttaactgtgc
     4741 tacagatgac acacaacaag cttcttcaag aacataataa tgcattgaaa acaattgagg
     4801 aactaacaag aaaagagtca gaaaaagtgg acagggcagt gctgaaggaa ctgagtgaaa
     4861 aactggaact ggcagagaag gctctggctt ccaaacagct gcaaatggat gaaatgaagc
     4921 aaaccattgc caagcaggaa gaggacctgg aaaccatgac catcctcagg gctcagatgg
     4981 aagtttactg ttctaatttt catgctgaaa gagcagcgag agagaaaatt catgaggaaa
     5041 aggagcaact ggcattgcag ctggcagttc tgctgaaaga gaatgatgct ttcgaagacg
     5101 gaggcaggca gtccttgatg gagatgcaga gtcgtcatgg ggcgagaaca agtgactctg
     5161 accagcaggc ttaccttgtt caaagaggag ctgaggacag ggactggcgg caacagcgga
     5221 atattccgat tcattcctgc cccaagtgtg gagaggttct gcctgacata gacacgttac
     5281 agattcacgt gatggattgc atcatttacc caactttctt gtacaaagtg gttcgataac
     5341 gaattctacc gggtagggga ggcgcttttc ccaaggcagt ctggagcatg cgctttagca
     5401 gccccgctgg gcacttggcg ctacacaagt ggcctctggc ctcgcacaca ttccacatcc
     5461 accggtaggc gccaaccggc tccgttcttt ggtggcccct tcgcgccacc ttctactcct
     5521 cccctagtca ggaagttccc ccccgccccg cagctcgcgt cgtgcaggac gtgacaaatg
     5581 gaagtagcac gtctcactag tctcgtgcag atggacagca ccgctgagca atggaagcgg
     5641 gtaggccttt ggggcagcgg ccaatagcag ctttgctcct tcgctttctg ggctcagagg
     5701 ctgggaaggg gtgggtccgg gggcgggctc aggggcgggc tcaggggcgg ggcgggcgcc
     5761 cgaaggtcct ccggaggccc ggcattctgc acgcttcaaa agcgcacgtc tgccgcgctg
     5821 ttctcctctt cctcatctcc gggcctttcg acctgcagcc cattaattaa accatggcca
     5881 agcctttgtc tcaagaagaa tccaccctca ttgaaagagc aacggctaca atcaacagca
     5941 tccccatctc tgaagactac agcgtcgcca gcgcagctct ctctagcgac ggccgcatct
     6001 tcactggtgt caatgtatat cattttactg ggggaccttg tgcagaactc gtggtgctgg
     6061 gcactgctgc tgctgcggca gctggcaacc tgacttgtat cgtcgcgatc ggaaatgaga
     6121 acaggggcat cttgagcccc tgcggacggt gccgacaggt gcttctcgat ctgcatcctg
     6181 ggatcaaagc catagtgaag gacagtgatg gacagccgac ggcagttggg attcgtgaat
     6241 tgctgccctc tggttatgtg tgggagggct aagcacttcg tggccgagga gcaggactga
     6301 cactcgacct cgaaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat
     6361 cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact
     6421 catcaatgta tcttatcatg tctatgcatc gatagatcct aatcaacctc tggattacaa
     6481 aatttgtgaa agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata
     6541 cgctgcttta atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc
     6601 cttgtataaa tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg
     6661 tggcgtggtg tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac
     6721 ctgtcagctc ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat
     6781 cgccgcctgc cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt
     6841 ggtgttgtcg gggaaatcat cgtcctttcc ttggctgctc gcctgtgttg ccacctggat
     6901 tctgcgcggg acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc
     6961 ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag
     7021 tcggatctcc ctttgggccg cctccccgcc tgagatcctt taagaccaat gacttacaag
     7081 gcagctgtag atcttagcca ctttttaaaa gaaaaggggg gactggaagg gctaattcac
     7141 tcccaacgaa gacaagatct gctttttgct tgtactgggt ctctctggtt agaccagatc
     7201 tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg
     7261 ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc
     7321 ctcagaccct tttagtcagt gtggaaaatc tctagcagta gtagttcatg tcatcttatt
     7381 attcagtatt tataacttgc aaagaaatga atatcagaga gtgagaggcc cgggttaatt
     7441 aaggaaaggg ctagatcatt cttgaagacg aaagggcctc gtgatacgcc tatttttata
     7501 ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt
     7561 gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag
     7621 acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca
     7681 tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc
     7741 agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat
     7801 cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc
     7861 aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg
     7921 gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc
     7981 agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat
     8041 aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga
     8101 gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc
     8161 ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc
     8221 aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt
     8281 aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc
     8341 tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc
     8401 agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca
     8461 ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca
     8521 ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt
     8581 ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta
     8641 acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg
     8701 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
     8761 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag
     8821 cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa
     8881 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc
     8941 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc
     9001 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta
     9061 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag
     9121 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct
     9181 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga
     9241 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc
     9301 ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt
     9361 atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg
     9421 cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg
     9481 caaaccgcct ctccccgcgc gttggccgat tcattaatgc agcaagctca tggctgacta
     9541 atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt ccagaagtag
     9601 tgaggaggct tttttggagg cctaggcttt tgcaaaaagc tccccgtggc acgacaggtt
     9661 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta
     9721 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg
     9781 ataacaattt cacacaggaa acagctatga catgattacg aatttcacaa ataaagcatt
     9841 tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg
     9901 gatcaactgg ataactcaag ctaaccaaaa tcatcccaaa cttcccaccc cataccctat
     9961 taccactgcc aattacctgt ggtttcattt actctaaacc tgtgattcct ctgaattatt
    10021 ttcattttaa agaaattgta tttgttaaat atgtactaca aacttagtag t
//
