LOCUS       sequence-346606-        5081 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of mCherry. Parton lab clone DFQ.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5081)
  AUTHORS   Lo HP, Lim YW, Xiong Z, Martel N, Ferguson C, Ariotti N, Giacomotto 
            J, Rae J, Floetenmeyer M, Moradi SV, Gao Y, Tillu VA, Xia D, Wang H,
            Rahnama S, Nixon SJ, Bastiani M, Day RD, Smith KA, Palpant NJ, 
            Johnston WA, Alexandrov K, Collins BM, Hall TE, Parton RG
  TITLE     Cavin4 interacts with Bin1 to promote T-tubule formation and 
            stability in developing skeletal muscle.
  JOURNAL   J Cell Biol. 2021 Dec 6;220(12). pii: 212693. doi: 
            10.1083/jcb.201905065. Epub 2021 Oct 11.
  PUBMED    34633413
REFERENCE   2  (bases 1 to 5081)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol."; date: "2021-12-6"; volume: "220(12). pii: 212693. doi"; 
            pages: " 10.1083/jcb.201905065. Epub 2021 Oct 11"
COMMENT     Sequence Label: mCherry
FEATURES             Location/Qualifiers
     source          1..5081
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        35..53
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     35..52
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     protein_bind    108..132
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             138..848
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(283..301)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     551..570
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     745..768
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     protein_bind    complement(850..874)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     polyA_signal    876..1010
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    complement(1073..1093)
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     polyA_signal    1133..1267
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1380..1400)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(1380..1398)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(1419..1435)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1419..1435)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1432..1454)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1443..1459
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1467..1473,1474..1491,1492..1497))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1512..1533
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1650..1667)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1821..2409)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1901..1920)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2580..3371,3372..3440))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3203..3222
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3441..3545)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(3571..4026)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3708..3729)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3920..3939
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        4097..5081
                     /label=CMV IE94 promoter
                     /note="enhancer/promoter region of simian cytomegalovirus 
                     major immediate early transcription unit IE94"
ORIGIN
        1 cgccattctg cctggggacg tcggagcaag cttgatttag gtgacactat agaatacaag
       61 ctacttgttc tttttgcagg atcccatcga ttcgaattca aggatcaaca agtttgtaca
      121 aaaaagcagg ctccaccatg gtgagcaagg gcgaggagga caacatggcc atcatcaagg
      181 agttcatgcg cttcaaggtg cacatggagg gctccgtgaa cggccacgag ttcgagatcg
      241 agggcgaggg cgagggccgc ccctacgagg gcacccagac cgccaagctg aaggtgacca
      301 agggcggccc cctgcccttc gcctgggaca tcctgtcccc tcagttcatg tacggctcca
      361 aggcctacgt gaagcacccc gccgacatcc ccgactactt gaagctgtcc ttccccgagg
      421 gcttcaagtg ggagcgcgtg atgaacttcg aggacggcgg cgtggtgacc gtgacccagg
      481 actcctccct gcaggacggc gagttcatct acaaggtgaa gctgcgcggc accaacttcc
      541 cctccgacgg ccccgtaatg cagaagaaga ccatgggctg ggaggcctcc tccgagcgga
      601 tgtaccccga ggacggcgcc ctgaagggcg agatcaagca gaggctgaag ctgaaggacg
      661 gcggccacta cgacgccgag gtcaagacca cctacaaggc caagaagccc gtgcagctgc
      721 ccggcgccta caacgtcaac atcaagctgg acatcacctc ccacaacgag gactacacca
      781 tcgtggaaca gtacgagcgc gccgagggcc gccactccac cggcggcatg gacgagctgt
      841 acaagtaaga cccagctttc ttgtacaaag tgggggatcc agacatgata agatacattg
      901 atgagtttgg acaaaccaca actagaatgc agtgaaaaaa atgctttatt tgtgaaattt
      961 gtgatgctat tgctttattt gtaaccatta taagctgcaa taaacaagtt aacaacaaca
     1021 attgcattca ttttatgttt caggttcagg gggaggtgtg ggaggttttt tccaacttta
     1081 ttatacatag ttgcactcga gcctctagaa ctatagtgag tcgtattacg tagatccaga
     1141 catgataaga tacattgatg agtttggaca aaccacaact agaatgcagt gaaaaaaatg
     1201 ctttatttgt gaaatttgtg atgctattgc tttatttgta accattataa gctgcaataa
     1261 acaagttaac aacaacaatt gcattcattt tatgtttcag gttcaggggg aggtgtggga
     1321 ggttttttaa ttcgcggccg cggcgccaat gcattgggcc cggtacccag cttttgttcc
     1381 ctttagtgag ggttaattgc gcgcttggcg taatcatggt catagctgtt tcctgtgtga
     1441 aattgttatc cgctcacaat tccacacaac atacgagccg ggagcataaa gtgtaaagcc
     1501 tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc
     1561 cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc
     1621 ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt
     1681 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca
     1741 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa
     1801 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat
     1861 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
     1921 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
     1981 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt
     2041 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac
     2101 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg
     2161 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca
     2221 gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc
     2281 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa
     2341 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa
     2401 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac
     2461 tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta
     2521 aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt
     2581 taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata
     2641 gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc
     2701 agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac
     2761 cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag
     2821 tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac
     2881 gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc
     2941 agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg
     3001 gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc
     3061 atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct
     3121 gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc
     3181 tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc
     3241 atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc
     3301 agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc
     3361 gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca
     3421 cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt
     3481 tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt
     3541 ccgcgcacat ttccccgaaa agtgccacct aaattgtaag cgttaatatt ttgttaaaat
     3601 tcgcgttaaa tttttgttaa atcagctcat tttttaacca ataggccgaa atcggcaaaa
     3661 tcccttataa atcaaaagaa tagaccgaga tagggttgag tgttgttcca gtttggaaca
     3721 agagtccact attaaagaac gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg
     3781 gcgatggccc actacgtgaa ccatcaccct aatcaagttt tttggggtcg aggtgccgta
     3841 aagcactaaa tcggaaccct aaagggagcc cccgatttag agcttgacgg ggaaagccgg
     3901 cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa
     3961 gtgtagcggt cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg ccgctacagg
     4021 gcgcgtccca ttcgccattc aggctgcgca actgttggga agggcgatcg gtgcgggcct
     4081 cttcgctatt acgccagtcg accgccaatt caatatggcg tatatggact catgccaatt
     4141 caatatggtg gatctggacc tgtgccaatt caatatggcg tatatggact cgtgccaatt
     4201 caatatggtg gatctggacc ccagccaatt caatatggcg gacttggcac catgccaatt
     4261 caatatggcg gacctggcac tgtgccaact ggggaggggt ctacttggca cggtgccaag
     4321 tttgaggagg ggtcttggcc ctgtgccaag tccgccatat tgaattggca tggtgccaat
     4381 aatggcggcc atattggcta tatgccagga tcaatatata ggcaatatcc aatatggccc
     4441 tatgccaata tggctattgg ccaggttcaa tactatgtat tggccctatg ccatatagta
     4501 ttccatatat gggttttcct attgacgtag atagcccctc ccaatgggcg gtcccatata
     4561 ccatatatgg ggcttcctaa taccgcccat agccactccc ccattgacgt caatggtctc
     4621 tatatatggt ctttcctatt gacgtcatat gggcggtcct attgacgtat atggcgcctc
     4681 ccccattgac gtcaattacg gtaaatggcc cgcctggctc aatgcccatt gacgtcaata
     4741 ggaccaccca ccattgacgt caatgggatg gctcattgcc cattcatatc cgttctcacg
     4801 ccccctattg acgtcaatga cggtaaatgg cccacttggc agtacatcaa tatctattaa
     4861 tagtaacttg gcaagtacat tactattgga agtacgccag ggtacattgg cagtactccc
     4921 attgacgtca atggcggtaa atggcccgcg atggctgcca agtacatccc cattgacgtc
     4981 aatggggagg ggcaatgacg caaatgggcg ttccattgac gtaaatgggc ggtaggcgtg
     5041 cctaatggga ggtctatata agcaatgctc gtttagggaa c
//
