LOCUS       sequence-346609-       10059 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector expressing N-terminally tagged eGFP-OPTN E50K.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10059)
  AUTHORS   Eapen VV, Swarup S, Hoyer MJ, Paulo JA, Harper JW
  TITLE     Quantitative proteomics reveals the selectivity of ubiquitin-binding
            autophagy receptors in the turnover of damaged lysosomes by 
            lysophagy.
  JOURNAL   eLife. 2021 Sep 29;10. pii: 72328. doi: 10.7554/eLife.72328.
  PUBMED    34585663
REFERENCE   2  (bases 1 to 10059)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "eLife."; 
            date: "2021-09-29"; volume: "10. pii: 72328. doi"; pages: " 
            10.7554/eLife.72328"
COMMENT     Sequence Label: pHAGE-eGFP-OPTN E50K
FEATURES             Location/Qualifiers
     source          1..10059
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2058..2175
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        2227..2530
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2531..2734
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2685..2705
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2731..2755
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      2814..2823
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(2820..2822,2823..2825,2826..3536)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(2820..2822,2823..2825,2826..3536)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2865..2886)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3126..3145)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3473..3494
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    3552..3576
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             3893..3904
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    complement(5308..5332)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        5351..5838
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(5386..5409)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     5771..5790
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             5862..6260
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     polyA_signal    6302..6423
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6339..6358)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     misc_feature    6449..7037
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6502..6522)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6920..6931)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7112..7345
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(7464..7482)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7550..7654
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7655..7723,7724..8515)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7873..7892)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8686..9274
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9175..9194
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9428..9445
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9685..9706
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9721..9726,9727..9744,9745..9751)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9759..9775
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9764..9786
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9783..9799
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9783..9799
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg
     2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa
     2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca
     2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactagtgga
     2221 gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg
     2281 cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg
     2341 acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca
     2401 tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc
     2461 ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc
     2521 tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc
     2581 acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa
     2641 tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag
     2701 gcgtgtacgg tgggaggtct atataagcag agctcgttta gtgaaccgtc agatcgcctg
     2761 gagacgccat ccacgctgtt ttgacctcca tagaagacac cggcggccga tctgccacca
     2821 tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc catcctggtc gagctggacg
     2881 gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg
     2941 gcaagctgac cctgaagttc atctgcacca ccggcaagct gcccgtgccc tggcccaccc
     3001 tcgtgaccac cctgacctac ggcgtgcagt gcttcagccg ctaccccgac cacatgaagc
     3061 agcacgactt cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc accatcttct
     3121 tcaaggacga cggcaactac aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg
     3181 tgaaccgcat cgagctgaag ggcatcgact tcaaggagga cggcaacatc ctggggcaca
     3241 agctggagta caactacaac agccacaacg tctatatcat ggccgacaag cagaagaacg
     3301 gcatcaaggt gaacttcaag atccgccaca acatcgagga cggcagcgtg cagctcgccg
     3361 accactacca gcagaacacc cccatcggcg acggccccgt gctgctgccc gacaaccact
     3421 acctgagcac ccagtccgcc ctgagcaaag accccaacga gaagcgcgat cacatggtcc
     3481 tgctggagtt cgtgaccgcc gccgggatca ctctcggcat ggacgagctg tacaaggctc
     3541 gaggttatca aacaagtttg tacaaaaaag ttggcatgtc ccatcaacct ctcagctgcc
     3601 tcactgaaaa ggaggacagc cccagtgaaa gcacaggaaa tggacccccc cacctggccc
     3661 acccaaacct ggacacgttt accccggagg agctgctgca gcagatgaaa gagctcctga
     3721 ccaagaacca ccagctgaaa gaagccatga agctaaataa tcaagccatg aaagggagat
     3781 ttgaggagct ttcggcctgg acagagaaac agaaggaaga acgccagttt tttgagatac
     3841 agagcaaaga agcaaaagag cgtctaatgg ccttgagtca tgagaatgag aaattgaagg
     3901 aagagcttgg aaaactaaaa gggaaatcag aaaggtcatc tgaggacccc actgatgact
     3961 ccaggcttcc cagggccgaa gcggagcagg aaaaggacca gctcaggacc caggtggtga
     4021 ggctacaagc agagaaggca gacctgttgg gcatcgtgtc tgaactgcag ctcaagctga
     4081 actccagcgg ctcctcagaa gattcctttg ttgaaattag gatggctgaa ggagaagcag
     4141 aagggtcagt aaaagaaatc aagcatagtc ctgggcccac gagaacagtc tccactggca
     4201 cggcattgtc taaatatagg agcagatctg cagatggggc caagaattac ttcgaacatg
     4261 aggagttaac tgtgagccag ctcctgctgt gcctaaggga agggaatcag aaggtggaga
     4321 gacttgaagt tgcactcaag gaggccaaag aaagagtttc agattttgaa aagaaaacaa
     4381 gtaatcgttc tgagattgaa acccagacag aggggagcac agagaaagag aatgatgaag
     4441 agaaaggccc ggagactgtt ggaagcgaag tggaagcact gaacctccag gtgacatctc
     4501 tgtttaagga gcttcaagag gctcatacaa aactcagcga agctgagcta atgaagaaga
     4561 gacttcaaga aaagtgtcag gcccttgaaa ggaaaaattc tgcaattcca tcagagttga
     4621 atgaaaagca agagcttgtt tatactaaca aaaagttaga gctacaagtg gaaagcatgc
     4681 tatcagaaat caaaatggaa caggctaaaa cagaggatga aaagtccaaa ttaactgtgc
     4741 tacagatgac acacaacaag cttcttcaag aacataataa tgcattgaaa acaattgagg
     4801 aactaacaag aaaagagtca gaaaaagtgg acagggcagt gctgaaggaa ctgagtgaaa
     4861 aactggaact ggcagagaag gctctggctt ccaaacagct gcaaatggat gaaatgaagc
     4921 aaaccattgc caagcaggaa gaggacctgg aaaccatgac catcctcagg gctcagatgg
     4981 aagtttactg ttctgatttt catgctgaaa gagcagcgag agagaaaatt catgaggaaa
     5041 aggagcaact ggcattgcag ctggcagttc tgctgaaaga gaatgatgct ttcgaagacg
     5101 gaggcaggca gtccttgatg gagatgcaga gtcgtcatgg ggcgagaaca agtgactctg
     5161 accagcaggc ttaccttgtt caaagaggag ctgaggacag ggactggcgg caacagcgga
     5221 atattccgat tcattcctgc cccaagtgtg gagaggttct gcctgacata gacacgttac
     5281 agattcacgt gatggattgc atcatttacc caactttctt gtacaaagtg gttcgataac
     5341 gaattctacc gggtagggga ggcgcttttc ccaaggcagt ctggagcatg cgctttagca
     5401 gccccgctgg gcacttggcg ctacacaagt ggcctctggc ctcgcacaca ttccacatcc
     5461 accggtaggc gccaaccggc tccgttcttt ggtggcccct tcgcgccacc ttctactcct
     5521 cccctagtca ggaagttccc ccccgccccg cagctcgcgt cgtgcaggac gtgacaaatg
     5581 gaagtagcac gtctcactag tctcgtgcag atggacagca ccgctgagca atggaagcgg
     5641 gtaggccttt ggggcagcgg ccaatagcag ctttgctcct tcgctttctg ggctcagagg
     5701 ctgggaaggg gtgggtccgg gggcgggctc aggggcgggg cgggcgcccg aaggtcctcc
     5761 ggaggcccgg cattctgcac gcttcaaaag cgcacgtctg ccgcgctgtt ctcctcttcc
     5821 tcatctccgg gcctttcgac ctgcagccca ttaattaaac catggccaag cctttgtctc
     5881 aagaagaatc caccctcatt gaaagagcaa cggctacaat caacagcatc cccatctctg
     5941 aagactacag cgtcgccagc gcagctctct ctagcgacgg ccgcatcttc actggtgtca
     6001 atgtatatca ttttactggg ggaccttgtg cagaactcgt ggtgctgggc actgctgctg
     6061 ctgcggcagc tggcaacctg acttgtatcg tcgcgatcgg aaatgagaac aggggcatct
     6121 tgagcccctg cggacggtgc cgacaggtgc ttctcgatct gcatcctggg atcaaagcca
     6181 tagtgaagga cagtgatgga cagccgacgg cagttgggat tcgtgaattg ctgccctctg
     6241 gttatgtgtg ggagggctaa gcacttcgtg gccgaggagc aggactgaca ctcgacctcg
     6301 aaacttgttt attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac
     6361 aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc
     6421 ttatcatgtc tatgcatcga tagatcctaa tcaacctctg gattacaaaa tttgtgaaag
     6481 attgactggt attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat
     6541 gcctttgtat catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc
     6601 ctggttgctg tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg
     6661 cactgtgttt gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct
     6721 ttccgggact ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct
     6781 tgcccgctgc tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg
     6841 gaaatcatcg tcctttcctt ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac
     6901 gtccttctgc tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct
     6961 gccggctctg cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc ggatctccct
     7021 ttgggccgcc tccccgcctg agatccttta agaccaatga cttacaaggc agctgtagat
     7081 cttagccact ttttaaaaga aaagggggga ctggaagggc taattcactc ccaacgaaga
     7141 caagatctgc tttttgcttg tactgggtct ctctggttag accagatctg agcctgggag
     7201 ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt
     7261 caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt
     7321 tagtcagtgt ggaaaatctc tagcagtagt agttcatgtc atcttattat tcagtattta
     7381 taacttgcaa agaaatgaat atcagagagt gagaggcccg ggttaattaa ggaaagggct
     7441 agatcattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat
     7501 gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc
     7561 tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg
     7621 ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc
     7681 ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt
     7741 gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct
     7801 caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac
     7861 ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact
     7921 cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa
     7981 gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga
     8041 taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt
     8101 tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga
     8161 agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg
     8221 caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat
     8281 ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat
     8341 tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc
     8401 agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga
     8461 tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc
     8521 agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag
     8581 gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc
     8641 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt
     8701 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt
     8761 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat
     8821 accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc
     8881 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa
     8941 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg
     9001 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag
     9061 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag
     9121 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa
     9181 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt
     9241 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg
     9301 gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc
     9361 tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac
     9421 cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct
     9481 ccccgcgcgt tggccgattc attaatgcag caagctcatg gctgactaat tttttttatt
     9541 tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt
     9601 tttggaggcc taggcttttg caaaaagctc cccgtggcac gacaggtttc ccgactggaa
     9661 agcgggcagt gagcgcaacg caattaatgt gagttagctc actcattagg caccccaggc
     9721 tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca
     9781 cacaggaaac agctatgaca tgattacgaa tttcacaaat aaagcatttt tttcactgca
     9841 ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgga tcaactggat
     9901 aactcaagct aaccaaaatc atcccaaact tcccacccca taccctatta ccactgccaa
     9961 ttacctgtgg tttcatttac tctaaacctg tgattcctct gaattatttt cattttaaag
    10021 aaattgtatt tgttaaatat gtactacaaa cttagtagt
//
