LOCUS       sequence-346610-        4671 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway entry clone with codon-optimized ORF sequence for subcloning
            into custom expression vectors. Contains a STOP codon at the end of 
            the codon-optimized ORF sequence. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4671)
  AUTHORS   Superti-Furga G, Lackner D, Wiedmer T, Ingles-Prieto A, Barbosa B, 
            Girardi E, Goldmann U, Gurtl B, Klavins K, Klimek C, Lindinger S, 
            Lineiro-Retes E, Muller AC, Onstein S, Redinger G, Reil D, Sedlyarov
            V, Wolf G, Crawford M, Everley R, ... Steppan CM
  TITLE     The RESOLUTE consortium: unlocking SLC transporters for drug 
            discovery.
  JOURNAL   Nat Rev Drug Discov. 2020 Jul;19(7):429-430. doi: 
            10.1038/d41573-020-00056-6.
  PUBMED    32265506
REFERENCE   2  (bases 1 to 4671)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Rev Drug Discov"; date: "2020-07"; volume: "19"; issue: "7"; 
            pages: "429-430"
COMMENT     Sequence Label: pDONR221-SLC44A2_STOP
FEATURES             Location/Qualifiers
     source          1..4671
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..100
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2228..2327)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2344..2363)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2345..2363)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2368..2384)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2368..2384)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2486..2505)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             2497..3306
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2564..2583)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      3453..4041
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3942..3961
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4195..4212
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(4371..4398)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(4490..4576)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     4625..4647
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4639..4656
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4640..4656
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacacat tgatgagcaa
       61 tgctttttta taatgccaac tttgtacaaa aaagcaggct ccaccatggg cgacgagcgg
      121 ccccactact atggcaagca cggcaccccc cagaagtacg accccacctt caagggccca
      181 atctataaca gaggctgcac cgatatcatc tgctgcgtgt tcctgctgct ggcaatcgtg
      241 ggatacgtgg cagtgggaat catcgcatgg acccacggcg accccagaaa agtgatctac
      301 cctacagata gccgcggcga gttctgtggc cagaagggca ccaagaacga gaataagcca
      361 tacctgttct acttcaacat cgtgaagtgc gcctcccccc tggtgctgct ggagtttcag
      421 tgtccaaccc cccagatctg cgtggagaag tgtcccgaca ggtacctgac atatctgaat
      481 gcccggagct ccagagattt cgagtactat aagcagtttt gcgtgcctgg cttcaagaac
      541 aataagggag tggcagaggt gctgcaggac ggcgattgcc ctgccgtgct gatcccctct
      601 aagcctctgg cccggagatg ttttccagcc atccacgcct acaagggcgt gctgatggtg
      661 ggcaacgaga ccacatatga ggacggccac ggcagccgga agaacatcac cgatctggtg
      721 gagggcgcca agaaggccaa tggcgtgctg gaggcaaggc agctggcaat gcgcatcttc
      781 gaggactaca cagtgtcctg gtattggatc atcatcggcc tggtcatcgc catggccatg
      841 tctctgctgt ttatcatcct gctgagattc ctggccggca tcatggtgtg ggtcatgatc
      901 atcatggtca tcctggtgct gggctacggc atctttcact gctacatgga gtatagcagg
      961 ctgaggggag aggcaggatc tgacgtgagc ctggtggatc tgggctttca gaccgatttc
     1021 cgggtgtacc tgcacctgag acagacatgg ctggccttta tgatcatcct gtccatcctg
     1081 gaagtgatca tcatcctgct gctgatcttc ctgcggaaga gaatcctgat cgccatcgcc
     1141 ctgatcaagg aggcatccag ggcagtggga tacgtgatgt gctctctgct gtatcctctg
     1201 gtgaccttct ttctgctgtg cctgtgcatc gcctactggg cctctaccgc cgtgtttctg
     1261 agcacatcca acgaggccgt gtataagatc tttgacgata gcccttgccc attcaccgcc
     1321 aagacatgta atcctgagac attcccatct agcaacgaga gcaggcagtg cccaaatgcc
     1381 cgctgtcagt tcgcctttta cggaggagag tccggatatc acagggccct gctgggactg
     1441 cagatcttca acgcctttat gttcttttgg ctggccaatt ttgtgctggc cctgggacag
     1501 gtgaccctgg caggagcctt cgccagctac tattgggccc tgcgcaagcc tgacgatctg
     1561 ccagccttcc ccctgttttc tgccttcggc agggccctga ggtaccacac aggaagcctg
     1621 gccttcggcg ccctgatcct ggccatcgtg cagatcatca gagtgatcct ggagtatctg
     1681 gatcagagac tgaaggccgc cgagaacaag tttgccaagt gcctgatgac ctgtctgaag
     1741 tgctgtttct ggtgcctgga gaagtttatc aagttcctga acaggaatgc ctacatcatg
     1801 atcgccatct atggcaccaa cttctgtaca tccgcccgca atgccttctt tctgctgatg
     1861 cggaatatca tcagagtggc cgtgctggac aaggtgaccg atttcctgtt tctgctgggc
     1921 aagctgctga tcgtgggctc tgtgggcatc ctggccttct ttttctttac ccacaggatc
     1981 cgcatcgtgc aggacacagc cccccctctg aactactatt gggtgccaat cctgaccgtg
     2041 atcgtgggca gctacctgat cgcccacggc ttcttttccg tgtatggcat gtgcgtggat
     2101 acactgttcc tgtgcttcct ggaggacctg gagcggaatg atggctccgc cgagagaccc
     2161 tacttcatgt cctctaccct gaagaagctg ctgaacaaga caaataagaa ggccgccgag
     2221 tcttaagacc cagctttctt gtacaaagtt ggcattataa gaaagcattg cttatcaatt
     2281 tgttgcaacg aacaggtcac tatcagtcaa aataaaatca ttatttgcca tccagctgat
     2341 atcccctata gtgagtcgta ttacatggtc atagctgttt cctggcagct ctggcccgtg
     2401 tctcaaaatc tctgatgtta cattgcacaa gataaaaata tatcatcatg aacaataaaa
     2461 ctgtctgctt acataaacag taatacaagg ggtgttatga gccatattca acgggaaacg
     2521 tcgaggccgc gattaaattc caacatggat gctgatttat atgggtataa atgggctcgc
     2581 gataatgtcg ggcaatcagg tgcgacaatc tatcgcttgt atgggaagcc cgatgcgcca
     2641 gagttgtttc tgaaacatgg caaaggtagc gttgccaatg atgttacaga tgagatggtc
     2701 agactaaact ggctgacgga atttatgcct cttccgacca tcaagcattt tatccgtact
     2761 cctgatgatg catggttact caccactgcg atccccggaa aaacagcatt ccaggtatta
     2821 gaagaatatc ctgattcagg tgaaaatatt gttgatgcgc tggcagtgtt cctgcgccgg
     2881 ttgcattcga ttcctgtttg taattgtcct tttaacagcg atcgcgtatt tcgtctcgct
     2941 caggcgcaat cacgaatgaa taacggtttg gttgatgcga gtgattttga tgacgagcgt
     3001 aatggctggc ctgttgaaca agtctggaaa gaaatgcata aacttttgcc attctcaccg
     3061 gattcagtcg tcactcatgg tgatttctca cttgataacc ttatttttga cgaggggaaa
     3121 ttaataggtt gtattgatgt tggacgagtc ggaatcgcag accgatacca ggatcttgcc
     3181 atcctatgga actgcctcgg tgagttttct ccttcattac agaaacggct ttttcaaaaa
     3241 tatggtattg ataatcctga tatgaataaa ttgcagtttc atttgatgct cgatgagttt
     3301 ttctaatcag aattggttaa ttggttgtaa cactggcaga gcattacgct gacttgacgg
     3361 gacggcgcaa gctcatgacc aaaatccctt aacgtgagtt acgcgtcgtt ccactgagcg
     3421 tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc
     3481 tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag
     3541 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt
     3601 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac
     3661 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc
     3721 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt
     3781 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt
     3841 gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc
     3901 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt
     3961 tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca
     4021 ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt
     4081 tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt
     4141 attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag
     4201 tcagtgagcg aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg
     4261 ccgattcatt aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc
     4321 aacgcaatta atacgcgtac cgctagccag gaagagtttg tagaaacgca aaaaggccat
     4381 ccgtcaggat ggccttctgc ttagtttgat gcctggcagt ttatggcggg cgtcctgccc
     4441 gccaccctcc gggccgttgc ttcacaacgt tcaaatccgc tcccggcgga tttgtcctac
     4501 tcaggagagc gttcaccgac aaacaacaga taaaacgaaa ggcccagtct tccgactgag
     4561 cctttcgttt tatttgatgc ctggcagttc cctactctcg cgttaacgct agcatggatg
     4621 ttttcccagt cacgacgttg taaaacgacg gccagtctta agctcgggcc c
//
