LOCUS       sequence-346611-        4122 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway entry clone with codon-optimized ORF sequence for subcloning
            into custom expression vectors. Contains a STOP codon at the end of 
            the codon-optimized ORF sequence. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4122)
  AUTHORS   Superti-Furga G, Lackner D, Wiedmer T, Ingles-Prieto A, Barbosa B, 
            Girardi E, Goldmann U, Gurtl B, Klavins K, Klimek C, Lindinger S, 
            Lineiro-Retes E, Muller AC, Onstein S, Redinger G, Reil D, Sedlyarov
            V, Wolf G, Crawford M, Everley R, ... Steppan CM
  TITLE     The RESOLUTE consortium: unlocking SLC transporters for drug 
            discovery.
  JOURNAL   Nat Rev Drug Discov. 2020 Jul;19(7):429-430. doi: 
            10.1038/d41573-020-00056-6.
  PUBMED    32265506
REFERENCE   2  (bases 1 to 4122)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Rev Drug Discov"; date: "2020-07"; volume: "19"; issue: "7"; 
            pages: "429-430"
COMMENT     Sequence Label: pDONR221-SLC35F5_STOP
FEATURES             Location/Qualifiers
     source          1..4122
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..100
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1679..1778)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1795..1814)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1796..1814)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1819..1835)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1819..1835)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1937..1956)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             1948..2757
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2015..2034)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      2904..3492
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3393..3412
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3646..3663
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(3822..3849)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(3941..4027)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     4076..4098
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4090..4107
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4091..4107
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacacat tgatgagcaa
       61 tgctttttta taatgccaac tttgtacaaa aaagcaggct ccaccatggt gccacctcgg
      121 agacacaggg gagcaggcag acccggcgtg ctgagctcct ctccaccctt tagactgagg
      181 tccgccaagt tctctggaat cgccctggag gatctgaggc gcgccctgaa gacaaggctg
      241 cagatggtgt gcgtgttcgt gatgaaccgc atgaattctc agaacagcgg ctttacccag
      301 cggagaagga tggccctggg catcgtgatc ctgctgctgg tggacgtgat ctgggtggcc
      361 agctccgagc tgacctccta cgtgttcaca cagtataata agcccttctt ttccaccttt
      421 gccaagacat ctatgttcgt gctgtacctg ctgggcttta tcatctggaa gccttggaga
      481 cagcagtgca caagaggcct gaggggcaag cacgccgcct tctttgccga tgccgagggc
      541 tacttcgccg cctgtaccac agacaccaca atgaactcta gcctgagcga gcctctgtat
      601 gtgccagtga agtttcacga tctgcccagc gagaagcctg agtccaccaa tatcgacacc
      661 gagaagacac ctaagaagtc tcgcgtgcgg ttcagcaaca tcatggagat ccggcagctg
      721 ccatcctctc acgccctgga ggccaagctg tccagaatgt cttaccccgt gaaggagcag
      781 gagtctatcc tgaagacagt gggcaagctg accgccacac aggtggccaa gatcagcttc
      841 tttttctgtt tcgtgtggtt cctggccaat ctgagctatc aggaggccct gtccgatacc
      901 caggtggcca tcgtgaacat cctgagctcc acctccggcc tgtttacact gatcctggcc
      961 gccgtgttcc catccaattc tggcgacagg tttacactgt ccaagctgct ggccgtgatc
     1021 ctgtctatcg gaggagtggt gctggtgaac ctggcaggaa gcgagaagcc agcaggacgc
     1081 gataccgtgg gaagcatctg gtccctggca ggagcaatgc tgtacgccgt gtatatcgtg
     1141 atgatcaagc ggaaggtgga cagagaggat aagctggaca tccctatgtt tttcggcttt
     1201 gtgggcctgt tcaatctgct gctgctgtgg ccaggctttt tcctgctgca ctacacaggc
     1261 ttcgaggact ttgagttccc caataaggtg gtgctgatgt gcatcatcat caacggcctg
     1321 atcggcaccg tgctgagcga gtttctgtgg ctgtggggct gtttcctgac ctctagcctg
     1381 atcggcacac tggccctgtc tctgaccatc cctctgagca tcatcgccga tatgtgcatg
     1441 cagaaggtgc agttcagctg gctgtttttc gccggcgcca tccccgtgtt cttcagcttc
     1501 ttcatcgtga ccctgctgtg ccactataac aattgggacc cagtgatggt gggcatccgc
     1561 cggatctttg ccttcatctg ccgcaagcac cggatccaga gagtgccaga ggattccgag
     1621 cagtgtgagt ctctgatcag catgcacagc gtgtcccagg aggacggagc atcttaagac
     1681 ccagctttct tgtacaaagt tggcattata agaaagcatt gcttatcaat ttgttgcaac
     1741 gaacaggtca ctatcagtca aaataaaatc attatttgcc atccagctga tatcccctat
     1801 agtgagtcgt attacatggt catagctgtt tcctggcagc tctggcccgt gtctcaaaat
     1861 ctctgatgtt acattgcaca agataaaaat atatcatcat gaacaataaa actgtctgct
     1921 tacataaaca gtaatacaag gggtgttatg agccatattc aacgggaaac gtcgaggccg
     1981 cgattaaatt ccaacatgga tgctgattta tatgggtata aatgggctcg cgataatgtc
     2041 gggcaatcag gtgcgacaat ctatcgcttg tatgggaagc ccgatgcgcc agagttgttt
     2101 ctgaaacatg gcaaaggtag cgttgccaat gatgttacag atgagatggt cagactaaac
     2161 tggctgacgg aatttatgcc tcttccgacc atcaagcatt ttatccgtac tcctgatgat
     2221 gcatggttac tcaccactgc gatccccgga aaaacagcat tccaggtatt agaagaatat
     2281 cctgattcag gtgaaaatat tgttgatgcg ctggcagtgt tcctgcgccg gttgcattcg
     2341 attcctgttt gtaattgtcc ttttaacagc gatcgcgtat ttcgtctcgc tcaggcgcaa
     2401 tcacgaatga ataacggttt ggttgatgcg agtgattttg atgacgagcg taatggctgg
     2461 cctgttgaac aagtctggaa agaaatgcat aaacttttgc cattctcacc ggattcagtc
     2521 gtcactcatg gtgatttctc acttgataac cttatttttg acgaggggaa attaataggt
     2581 tgtattgatg ttggacgagt cggaatcgca gaccgatacc aggatcttgc catcctatgg
     2641 aactgcctcg gtgagttttc tccttcatta cagaaacggc tttttcaaaa atatggtatt
     2701 gataatcctg atatgaataa attgcagttt catttgatgc tcgatgagtt tttctaatca
     2761 gaattggtta attggttgta acactggcag agcattacgc tgacttgacg ggacggcgca
     2821 agctcatgac caaaatccct taacgtgagt tacgcgtcgt tccactgagc gtcagacccc
     2881 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
     2941 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
     3001 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg
     3061 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
     3121 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
     3181 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
     3241 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga
     3301 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
     3361 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
     3421 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
     3481 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct
     3541 tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc
     3601 tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc
     3661 gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat
     3721 taatgcagct ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt
     3781 aatacgcgta ccgctagcca ggaagagttt gtagaaacgc aaaaaggcca tccgtcagga
     3841 tggccttctg cttagtttga tgcctggcag tttatggcgg gcgtcctgcc cgccaccctc
     3901 cgggccgttg cttcacaacg ttcaaatccg ctcccggcgg atttgtccta ctcaggagag
     3961 cgttcaccga caaacaacag ataaaacgaa aggcccagtc ttccgactga gcctttcgtt
     4021 ttatttgatg cctggcagtt ccctactctc gcgttaacgc tagcatggat gttttcccag
     4081 tcacgacgtt gtaaaacgac ggccagtctt aagctcgggc cc
//
