LOCUS       sequence-346613-        3816 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway entry clone with codon-optimized ORF sequence for subcloning
            into custom expression vectors. Contains a STOP codon at the end of 
            the codon-optimized ORF sequence. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3816)
  AUTHORS   Superti-Furga G, Lackner D, Wiedmer T, Ingles-Prieto A, Barbosa B, 
            Girardi E, Goldmann U, Gurtl B, Klavins K, Klimek C, Lindinger S, 
            Lineiro-Retes E, Muller AC, Onstein S, Redinger G, Reil D, Sedlyarov
            V, Wolf G, Crawford M, Everley R, ... Steppan CM
  TITLE     The RESOLUTE consortium: unlocking SLC transporters for drug 
            discovery.
  JOURNAL   Nat Rev Drug Discov. 2020 Jul;19(7):429-430. doi: 
            10.1038/d41573-020-00056-6.
  PUBMED    32265506
REFERENCE   2  (bases 1 to 3816)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Rev Drug Discov"; date: "2020-07"; volume: "19"; issue: "7"; 
            pages: "429-430"
COMMENT     Sequence Label: pDONR221-SLC35F3_STOP
FEATURES             Location/Qualifiers
     source          1..3816
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..100
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1373..1472)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1489..1508)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1490..1508)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1513..1529)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1513..1529)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1631..1650)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             1642..2451
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1709..1728)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      2598..3186
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3087..3106
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3340..3357
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(3516..3543)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(3635..3721)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     3770..3792
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3784..3801
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3785..3801
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacacat tgatgagcaa
       61 tgctttttta taatgccaac tttgtacaaa aaagcaggct ccaccatgaa gaagcactcc
      121 gccagggtgg caccactgtc tgcctgcaat agccccgtgc tgaccctgac aaaggtggag
      181 ggagaggaga ggccaagaga ctctccagga cctgcagagg cacaggcacc tgcaggcgtg
      241 gaggccggcg gcagggcctc tcggagatgc tggacctgta gccgcgccca gctgaagaag
      301 atcttctggg gagtggcagt ggtgctgtgc gtgtgcagct cctgggcagg atccacccag
      361 ctggccaagc tgacattccg gaagtttgac gcccctttca ccctgacatg gtttgccacc
      421 aactggaatt ttctgttctt tccactgtac tatgtgggcc acgtgtgcaa gtccacagag
      481 aagcagtctg tgaagcagag gtaccgcgag tgctgtagat tctttggcga taacggcctg
      541 accctgaagg tgttctttac aaaggcagca cctttcggcg tgctgtggac cctgacaaac
      601 tacctgtatc tgcacgccat caagaagatc aataccacag acgtgtctgt gctgttctgc
      661 tgtaacaagg ccttcgtgtt tctgctgagc tggatcgtgc tgcgggatcg gttcatgggc
      721 gtgaggatcg tggcagcaat cctggcaatc gcaggcatcg tgatgatgac atacgccgac
      781 ggcttccaca gccactccgt gatcggaatc gccctggtgg tggcatctgc cagcatgtcc
      841 gccctgtata aggtgctgtt taagctgctg ctgggaagcg ccaagttcgg agaggccgcc
      901 ctgtttctgt ccatcctggg cgtgttcaat atcctgttta tcacctgcat ccccatcatc
      961 ctgtacttca caaaggtgga gtattggtct agctttgacg atatcccttg gggcaacctg
     1021 tgcggcttct ccgtgctgct gctgaccttc aacatcgtgc tgaattttgg catcgccgtg
     1081 acctacccaa cactgatgag cctgggcatc gtgctgtcca tccccgtgaa tgccgtgatc
     1141 gatcactata caagccagat cgtgttcaac ggcgtgcgcg tgatcgccat catcatcatc
     1201 ggcctgggct ttctgctgct gctgctgcca gaggagtggg acgtgtggct gatcaagctg
     1261 ctgaccaggc tgaaggtgcg caagaaggag gagccagcag agggagcagc agatctgtcc
     1321 tctggccctc agtccaagaa taggcgcgcc cggccatctt ttgccagata agacccagct
     1381 ttcttgtaca aagttggcat tataagaaag cattgcttat caatttgttg caacgaacag
     1441 gtcactatca gtcaaaataa aatcattatt tgccatccag ctgatatccc ctatagtgag
     1501 tcgtattaca tggtcatagc tgtttcctgg cagctctggc ccgtgtctca aaatctctga
     1561 tgttacattg cacaagataa aaatatatca tcatgaacaa taaaactgtc tgcttacata
     1621 aacagtaata caaggggtgt tatgagccat attcaacggg aaacgtcgag gccgcgatta
     1681 aattccaaca tggatgctga tttatatggg tataaatggg ctcgcgataa tgtcgggcaa
     1741 tcaggtgcga caatctatcg cttgtatggg aagcccgatg cgccagagtt gtttctgaaa
     1801 catggcaaag gtagcgttgc caatgatgtt acagatgaga tggtcagact aaactggctg
     1861 acggaattta tgcctcttcc gaccatcaag cattttatcc gtactcctga tgatgcatgg
     1921 ttactcacca ctgcgatccc cggaaaaaca gcattccagg tattagaaga atatcctgat
     1981 tcaggtgaaa atattgttga tgcgctggca gtgttcctgc gccggttgca ttcgattcct
     2041 gtttgtaatt gtccttttaa cagcgatcgc gtatttcgtc tcgctcaggc gcaatcacga
     2101 atgaataacg gtttggttga tgcgagtgat tttgatgacg agcgtaatgg ctggcctgtt
     2161 gaacaagtct ggaaagaaat gcataaactt ttgccattct caccggattc agtcgtcact
     2221 catggtgatt tctcacttga taaccttatt tttgacgagg ggaaattaat aggttgtatt
     2281 gatgttggac gagtcggaat cgcagaccga taccaggatc ttgccatcct atggaactgc
     2341 ctcggtgagt tttctccttc attacagaaa cggctttttc aaaaatatgg tattgataat
     2401 cctgatatga ataaattgca gtttcatttg atgctcgatg agtttttcta atcagaattg
     2461 gttaattggt tgtaacactg gcagagcatt acgctgactt gacgggacgg cgcaagctca
     2521 tgaccaaaat cccttaacgt gagttacgcg tcgttccact gagcgtcaga ccccgtagaa
     2581 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca
     2641 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt
     2701 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg
     2761 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc
     2821 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga
     2881 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc
     2941 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc
     3001 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca
     3061 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg
     3121 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta
     3181 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct
     3241 cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag
     3301 tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa
     3361 gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc
     3421 agctggcacg acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatacg
     3481 cgtaccgcta gccaggaaga gtttgtagaa acgcaaaaag gccatccgtc aggatggcct
     3541 tctgcttagt ttgatgcctg gcagtttatg gcgggcgtcc tgcccgccac cctccgggcc
     3601 gttgcttcac aacgttcaaa tccgctcccg gcggatttgt cctactcagg agagcgttca
     3661 ccgacaaaca acagataaaa cgaaaggccc agtcttccga ctgagccttt cgttttattt
     3721 gatgcctggc agttccctac tctcgcgtta acgctagcat ggatgttttc ccagtcacga
     3781 cgttgtaaaa cgacggccag tcttaagctc gggccc
//
