LOCUS       sequence-346615-       10707 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector expressing N-terminally tagged eGFP-TAX1BP1 A114Q.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10707)
  AUTHORS   Eapen VV, Swarup S, Hoyer MJ, Paulo JA, Harper JW
  TITLE     Quantitative proteomics reveals the selectivity of ubiquitin-binding
            autophagy receptors in the turnover of damaged lysosomes by 
            lysophagy.
  JOURNAL   eLife. 2021 Sep 29;10. pii: 72328. doi: 10.7554/eLife.72328.
  PUBMED    34585663
REFERENCE   2  (bases 1 to 10707)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "eLife."; 
            date: "2021-09-29"; volume: "10. pii: 72328. doi"; pages: " 
            10.7554/eLife.72328"
COMMENT     Sequence Label: pHAGE-eGFP-TAX1BP1 A114Q
FEATURES             Location/Qualifiers
     source          1..10707
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(91..590)
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(139..158)
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     primer_bind     532..555
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     protein_bind    609..633
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(3001..3025)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(join(3041..3751,3752..3754,3755..3757))
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             complement(join(3041..3751,3752..3754,3755..3757))
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3083..3104)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     3432..3451
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3691..3712
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      3754..3763
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(3822..3846)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        complement(3843..4046)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(3872..3892)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        4047..4350
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     misc_feature    4402..4519
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(4812..4856)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    5041..5274
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    5771..5896
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             5943..6576
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(6837..6853)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6837..6853)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6850..6872)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6861..6877
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6885..6891,6892..6909,6910..6915))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6930..6951
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7191..7208)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7362..7950)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7442..7461)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8121..8912,8913..8981))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8744..8763
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8982..9086)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     9154..9172
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     LTR             9291..9524
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     misc_feature    9599..10187
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             9705..9716
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     10114..10134
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     polyA_signal    10213..10334
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     10278..10297
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(join(10376..10707,1..67))
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
ORIGIN
        1 tgctgttgat tgtagccgtt gctctttcaa tgagggtgga ttcttcttga gacaaaggct
       61 tggccatggt ttaattaatg ggctgcaggt cgaaaggccc ggagatgagg aagaggagaa
      121 cagcgcggca gacgtgcgct tttgaagcgt gcagaatgcc gggcctccgg aggaccttcg
      181 ggcgcccgcc ccgcccctga gcccgcccct gagcccgccc ccggacccac cccttcccag
      241 cctctgagcc cagaaagcga aggagcaaag ctgctattgg ccgctgcccc aaaggcctac
      301 ccgcttccat tgctcagcgg tgctgtccat ctgcacgaga ctagtgagac gtgctacttc
      361 catttgtcac gtcctgcacg acgcgagctg cggggcgggg gggaacttcc tgactagggg
      421 aggagtagaa ggtggcgcga aggggccacc aaagaacgga gccggttggc gcctaccggt
      481 ggatgtggaa tgtgtgcgag gccagaggcc acttgtgtag cgccaagtgc ccagcggggc
      541 tgctaaagcg catgctccag actgccttgg gaaaagcgcc tcccctaccc ggtagaattc
      601 gttatcgaac cactttgtac aagaaagttg ggtagtcaaa atttagaaca ttctgatcaa
      661 aatgggtctg cacatgcctt tcaaacacct gctggtcata gtcaggaggg aactgctcgc
      721 tgcacatcgg gcacaccttc cagtgacttt caacatgttc ttcaaatttg ctctgatcat
      781 agttaggagg aaacattaac tcacagaggg gacacttctt gtgaacatca aagctggaat
      841 caaagcaaaa gcctgtccca tgcccacgta aatgttgact tggaggatca ggagcagtag
      901 gcacattctc atcttctttg ctatcctcag agtcctctaa gccatcaggc cgactaaagt
      961 ttcgagcagg ctggctgcag acaacattgt cttccagtcc ccaagagggg actctgacag
     1021 gtggcctttg tatttcatct gggtaaaaag caccatctgc tccatctctt gtttcctgag
     1081 atgcataagg attaccatat tgcagaactg gttgtgcatt tgacaatgtg agaacataac
     1141 cattttgctc tgagcatgtt ttgaaacatt gaggactctg ggaattctga ccttccattt
     1201 tcctttctgc tggattttct agactccttt taagttcttt ataattgtcc tgtacttcag
     1261 ctagttcaag ttttacattt tcagcaattt tcacttgttc tttccatttc agctccattt
     1321 ttgcaagttc atcagcatat ttattgcatt ttgctttctc atcctgcaag agttgtttac
     1381 atttattata cttttctgtt ttgtcagcaa tttctttggt catttcacag acttgcccct
     1441 ttgttactat gtcaaaatct gcctctgctg cagaaggtga tggctttaca tctacagtag
     1501 aggcagaagt ggctgggtct gtgtttactg aagcatcatt cactttctgc tgattccccg
     1561 ttttctttgt gaagacatta ttattattag ctgattgatc tgaaagtttg tttatttgtt
     1621 tttggagcct ttggcattcc ttaaattttt ctttataatg gtctgcagcc atctgaagac
     1681 ggagtttcag atcttcaact tctcttctta gttcgtgttc cagtgtatca gtcttgtcct
     1741 gatctttttt catagcattt agtttaagtt ctgccactgc atcagctaac tgctttttca
     1801 ctttctcgtt ttccaagcgt gcagtatgca ggtctgccat cgttctgtct cgtacgttga
     1861 cagcatcact gagttcttta gccagaaaga caacttcttg ccgagttgcc tgaacctgtt
     1921 cctctgcttt acgaagttgc tcctttaaaa aacaagtatc ttctttactt gaggttgtaa
     1981 gcaggaaagt tctttgcaga ttttcctttt cagccaaaca taactgcagc ctgccaatct
     2041 cttctttgaa atgagtaatc acgctttctt tgttcccatc taaatttttt aaagtctgga
     2101 cctctgacat aagcttggta ttttctattt ctgtattctt caaatgtacc ttataaagtt
     2161 ccttttcatc cttctctgtc ttcaactgac attcaagttg ttctctttca tgttgtgcct
     2221 tcttgagttt gtcctttaaa ctgtctaatt cggtttcttt ttcaattgct ttatgtgtta
     2281 ctgacacaat atcttcctca agctgatggg ctttggatgt agcatcactg aacctcttct
     2341 taaactcttc attttccatt tttaagcttt gtgttacttc agtaagaccc ttttgttctg
     2401 cttgcagttg gtcacatctt tctttctcat ggttaagttc tctttccatt ctcccaactt
     2461 gttctcgaag ttgtgctgtt tctttttcca gaacggcaat taactttaac agttcttctt
     2521 tttctttcat ggttttctca attttcaact caagaaggcc tgcttttgtg gtcaccacta
     2581 acatgtcaga atttccttca tcttccatag taagcagctc ttcaactgga gaagaagctc
     2641 gaaactggaa aggtgtactc tgtccacgaa tttcaccctt atgggtaacg taacagaact
     2701 gataaaattc tccatcatca tttggaaggt aatatccttg gaatgctagt acacaattga
     2761 ctgttgatcc ttccacataa tgttcaggca taggggacca taaaaacgtg taataatcac
     2821 gagcagtact ccatccaacc ttgaatatac caacccaatc ttttggatgt ggatgaatat
     2881 atggagttaa ggtgtaatga cattccaggt gtgcattagg aaggtaactc ttggccacat
     2941 tttgaaagat gacatgggca aagttggaag tctgcaatgg gacttcttga aaggatgtca
     3001 tgccaacttt tttgtacaaa cttgtttgat aacctcgagc cttgtacagc tcgtccatgc
     3061 cgagagtgat cccggcggcg gtcacgaact ccagcaggac catgtgatcg cgcttctcgt
     3121 tggggtcttt gctcagggcg gactgggtgc tcaggtagtg gttgtcgggc agcagcacgg
     3181 ggccgtcgcc gatgggggtg ttctgctggt agtggtcggc gagctgcacg ctgccgtcct
     3241 cgatgttgtg gcggatcttg aagttcacct tgatgccgtt cttctgcttg tcggccatga
     3301 tatagacgtt gtggctgttg tagttgtact ccagcttgtg ccccaggatg ttgccgtcct
     3361 ccttgaagtc gatgcccttc agctcgatgc ggttcaccag ggtgtcgccc tcgaacttca
     3421 cctcggcgcg ggtcttgtag ttgccgtcgt ccttgaagaa gatggtgcgc tcctggacgt
     3481 agccttcggg catggcggac ttgaagaagt cgtgctgctt catgtggtcg gggtagcggc
     3541 tgaagcactg cacgccgtag gtcagggtgg tcacgagggt gggccagggc acgggcagct
     3601 tgccggtggt gcagatgaac ttcagggtca gcttgccgta ggtggcatcg ccctcgccct
     3661 cgccggacac gctgaacttg tggccgttta cgtcgccgtc cagctcgacc aggatgggca
     3721 ccaccccggt gaacagctcc tcgcccttgc tcaccatggt ggcagatcgg ccgccggtgt
     3781 cttctatgga ggtcaaaaca gcgtggatgg cgtctccagg cgatctgacg gttcactaaa
     3841 cgagctctgc ttatatagac ctcccaccgt acacgcctac cgcccatttg cgtcaatggg
     3901 gcggagttgt tacgacattt tggaaagtcc cgttgatttt ggtgccaaaa caaactccca
     3961 ttgacgtcaa tggggtggag acttggaaat ccccgtgagt caaaccgcta tccacgccca
     4021 ttgatgtact gccaaaaccg catcaccatg gtaatagcga tgactaatac gtagatgtac
     4081 tgccaagtag gaaagtccca taaggtcatg tactgggcat aatgccaggc gggccattta
     4141 ccgtcattga cgtcaatagg gggcgtactt ggcatatgat acacttgatg tactgccaag
     4201 tgggcagttt accgtaaata ctccacccat tgacgtcaat ggaaagtccc tattggcgtt
     4261 actatgggaa catacgtcat tattgacgtc aatgggcggg ggtcgttggg cggtcagcca
     4321 ggcgggccat ttaccgtaag ttatgtaacg cggaactcca ctagtccaaa ctggatctct
     4381 gctgtccctg taataaaccc gaaaattttg aatttttgta atttgttttt gtaattcttt
     4441 agtttgtatg tctgttgcta ttatgtctac tattctttcc cctgcactgt accccccaat
     4501 cccccctttt cttttaaaat tgtggatgaa tactgccatt tgtgaattcg gcgataccgt
     4561 cgagatccgt tcactaatcg aatggatctg tctctgtctc tctctccacc ttcttcttct
     4621 attccttcgg gcctgtcggg tcccctcggg gttgggaggt gggtctgaaa cgataatggt
     4681 gaatatccct gcctaactct attcactata gaaagtacag caaaaactat tcttaaacct
     4741 accaagcctc ctactatcat tatgaataat tttatatacc acagccaatt tgttatgtta
     4801 aaccaattcc acaaacttgc ccatttatct aattccaata attcttgttc attcttttct
     4861 tgctggtttt gcgattcttc aattaaggag tgtattaagc ttgtgtaatt gttaatttct
     4921 ctgtcccact ccatccaggt cgtgtgattc caaatctgtt ccagagattt attactccaa
     4981 ctagcattcc aaggcacagc agtggtgcaa atgagttttc cagagcaacc ccaaatcccc
     5041 aggagctgtt gatcctttag gtatctttcc acagccagga ttcttgcctg gagctgcttg
     5101 atgccccaga ctgtgagttg caacagatgc tgttgcgcct caatagccct cagcaaattg
     5161 ttctgctgct gcactatacc agacaataat tgtctggcct gtaccgtcag cgtcattgac
     5221 gctgcgccca tagtgcttcc tgctgctccc aagaacccaa ggaacaaagc tcctattccc
     5281 actgctcttt tttctctctg caccactctt ctctttgcct tggtgggtgc tactcctaat
     5341 ggttcaattt ttactacttt atatttatat aattcacttc tccaattgtc cctcatatct
     5401 cctcctccag gtctgaagat cagcggccgg ccgcttgctg tgcggtggtc ttacttttgt
     5461 tttgctcttc ctctatcttg tctaaagctt ccttggtgtc ttttatctct atcctttgat
     5521 gcacacaata gagggttgct actgtattat ataatgatct aagttcttct gatcctgtct
     5581 gaagggatgg ttgtagctgt cccagtattt gtctacagcc ttctgatgtt tctaacaggc
     5641 caggattaac tgcgaatcgt tctagctccc tgcttgccca tactatatgt tttaatttat
     5701 attttttctt tccccctggc cttaaccgaa ttttttccca tcgcgatcta attctccccc
     5761 gcttaatact gacgctctcg cacccatctc tctccttcta gcctccgcta gtcaaaattt
     5821 ttggcgtact caccagtcgc cgcccctcgc ctcttgccgt gcgcgcttca gcaagccgag
     5881 tcctgcgtcg agagagctcc tctggtttcc ctttcgcttt caagtccctg ttcgggcgcc
     5941 actgctagag attttccaca ctgactaaaa gggtctgagg gatctctagt taccagagtc
     6001 acacaacaga cgggcacaca ctacttgaag cactcaaggc aagctttatt gaggcttaag
     6061 cagtgggttc cctagttagc cagagagctc ccaggctcag atctggtcta accagagaga
     6121 cccagtacag gcaaaaagca gctgcttata tgcaggatct gagggctcgc cactccccag
     6181 tcccgcccag gccacgcctc cctggaaagt ccccagcgga aagtcccttg tagcaagctc
     6241 gatatcagca gttcttgaag tactccggat gcagctctcg ggccacgtga tgaaatgcta
     6301 ggcggctgtc aaacctccac tctaacactt ctctctccgg gtcatccatc ccatgcaggc
     6361 tcacagggtg taacaagctg gtgttctctc ctttattggc ctcttctacc ttatctggct
     6421 caactggtac tagcttgtag caccatccaa aggtcagtgg atatctgacc cctggccctg
     6481 gtgtgtagtt ctgctaatca gggaagtagc cttgtgtgtg gtagatccac agatcaagga
     6541 tatcttgtct tctttgggag tgaattagcc cttccaacta ctaagtttgt agtacatatt
     6601 taacaaatac aatttcttta aaatgaaaat aattcagagg aatcacaggt ttagagtaaa
     6661 tgaaaccaca ggtaattggc agtggtaata gggtatgggg tgggaagttt gggatgattt
     6721 tggttagctt gagttatcca gttgatccag acatgataag atacattgat gagtttggac
     6781 aaaccacaac tagaatgcag tgaaaaaaat gctttatttg tgaaattcgt aatcatgtca
     6841 tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga
     6901 agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg
     6961 cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc acggggagct ttttgcaaaa
     7021 gcctaggcct ccaaaaaagc ctcctcacta cttctggaat agctcagagg ccgaggcggc
     7081 ctcggcctct gcataaataa aaaaaattag tcagccatga gcttgctgca ttaatgaatc
     7141 ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact
     7201 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta
     7261 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag
     7321 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
     7381 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
     7441 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
     7501 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc
     7561 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
     7621 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
     7681 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
     7741 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
     7801 agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
     7861 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
     7921 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct
     7981 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg
     8041 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat
     8101 gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc
     8161 tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg
     8221 gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct
     8281 ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca
     8341 actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg
     8401 ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg
     8461 tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc
     8521 cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag
     8581 ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg
     8641 ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag
     8701 tgtatgcggc gaccgagttg ctcttgcccg gcgtcaacac gggataatac cgcgccacat
     8761 agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg
     8821 atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca
     8881 gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca
     8941 aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat
     9001 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag
     9061 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa
     9121 gaaaccatta ttatcatgac attaacctat aaaaataggc gtatcacgag gccctttcgt
     9181 cttcaagaat gatctagccc tttccttaat taacccgggc ctctcactct ctgatattca
     9241 tttctttgca agttataaat actgaataat aagatgacat gaactactac tgctagagat
     9301 tttccacact gactaaaagg gtctgaggga tctctagtta ccagagtcac acaacagacg
     9361 ggcacacact acttgaagca ctcaaggcaa gctttattga ggcttaagca gtgggttccc
     9421 tagttagcca gagagctccc aggctcagat ctggtctaac cagagagacc cagtacaagc
     9481 aaaaagcaga tcttgtcttc gttgggagtg aattagccct tccagtcccc ccttttcttt
     9541 taaaaagtgg ctaagatcta cagctgcctt gtaagtcatt ggtcttaaag gatctcaggc
     9601 ggggaggcgg cccaaaggga gatccgactc gtctgagggc gaaggcgaag acgcggaaga
     9661 ggccgcagag ccggcagcag gccgcgggaa ggaaggtccg ctggattgag ggccgaaggg
     9721 acgtagcaga aggacgtccc gcgcagaatc caggtggcaa cacaggcgag cagccaagga
     9781 aaggacgatg atttccccga caacaccacg gaattgtcag tgcccaacag ccgagcccct
     9841 gtccagcagc gggcaaggca ggcggcgatg agttccgccg tggcaatagg gagggggaaa
     9901 gcgaaagtcc cggaaaggag ctgacaggtg gtggcaatgc cccaaccagt gggggttgcg
     9961 tcagcaaaca cagtgcacac cacgccacgt tgcctgacaa cgggccacaa ctcctcataa
    10021 agagacagca accaggattt atacaaggag gagaaaatga aagccatacg ggaagcaata
    10081 gcatgataca aaggcattaa agcagcgtat ccacatagcg taaaaggagc aacatagtta
    10141 agaataccag tcaatctttc acaaattttg taatccagag gttgattagg atctatcgat
    10201 gcatagacat gataagatac attgatgagt ttggacaaac cacaactaga atgcagtgaa
    10261 aaaaatgctt tatttgtgaa atttgtgatg ctattgcttt atttgtaacc attataagct
    10321 gcaataaaca agtttcgagg tcgagtgtca gtcctgctcc tcggccacga agtgcttagc
    10381 cctcccacac ataaccagag ggcagcaatt cacgaatccc aactgccgtc ggctgtccat
    10441 cactgtcctt cactatggct ttgatcccag gatgcagatc gagaagcacc tgtcggcacc
    10501 gtccgcaggg gctcaagatg cccctgttct catttccgat cgcgacgata caagtcaggt
    10561 tgccagctgc cgcagcagca gcagtgccca gcaccacgag ttctgcacaa ggtcccccag
    10621 taaaatgata tacattgaca ccagtgaaga tgcggccgtc gctagagaga gctgcgctgg
    10681 cgacgctgta gtcttcagag atgggga
//
