LOCUS       sequence-346624-        7778 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression vector for E. coli producing target protein with 
            N-terminal Nanoluciferase fusion.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7778)
  AUTHORS   Sowa ST, Galera-Prat A, Wazir S, Alanen HI, Maksimainen MM, Lehtio L
  TITLE     A molecular toolbox for ADP-ribosyl binding proteins.
  JOURNAL   Cell Rep Methods. 2021 Dec 20;1(8):100121. doi: 
            10.1016/j.crmeth.2021.100121. Epub 2021 Nov 11.
  PUBMED    34786571
REFERENCE   2  (bases 1 to 7778)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep 
            Methods."; date: "2021-12-20"; volume: "1(8):100121. doi"; pages: " 
            10.1016/j.crmeth.2021.100121. Epub 2021 Nov 11"
COMMENT     Sequence Label: pNH-NanoLuc
FEATURES             Location/Qualifiers
     source          1..7778
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     RBS             12..34
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             45..62
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             66..578
                     /codon_start=1
                     /product="NanoLuc(R) luciferase"
                     /label=Nluc
                     /note="human codon-optimized"
                     /translation="MVFTLEDFVGDWRQTAGYNLDQVLEQGGVSSLFQNLGVSVTPIQR
                     IVLSGENGLKIDIHVIIPYEGLSGDQMGQIEKIFKVVYPVDDHHFKVILHYGTLVIDGV
                     TPNMIDYFGRPYEGIAVFDGKKITVTGTLWNGNKIIDERLINPDGSLLFRVTINGVTGW
                     RLCERILA"
     CDS             585..605
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     promoter        621..1066
                     /gene="sacR"
                     /label=sacB promoter
                     /note="sacB promoter and control region"
     CDS             join(1067..1153,1154..2488)
                     /codon_start=1
                     /gene="Bacillus subtilis sacB"
                     /product="secreted levansucrase that renders bacterial 
                     growth sensitive to sucrose"
                     /label=SacB
                     /note="negative selection marker"
                     /translation="MNIKKFAKQATVLTFTTALLAGGATQAFAKETNQKPYKETYGISH
                     ITRHDMLQIPEQQKNEKYKVPEFDSSTIKNISSAKGLDVWDSWPLQNTDGTVANYHGYH
                     IVFALAGDPKNADDTSIYMFYQKVGETSIDSWKNAGRVFKDSDKFDANDSILKDQTQEW
                     SGSATFTSDGKIRLFYTDFSGKHYGKQTLTTAQVNVSASDSSLNINGVEDYKSIFDGDG
                     KTYQNVQQFIDEGNYSSGDNHTLRDPHYVEDKGHKYLVFEANTGTEDGYQGEESLFNKA
                     YYGKSTSFFRQESQKLLQSDKKRTAELANGALGMIELNDDYTLKKVMKPLIASNTVTDE
                     IERANVFKMNGKWYLFTDSRGSKMTIDGITSNDIYMLGYVSNSLTGPYKPLNKTGLVLK
                     MDLDPNDVTFTYSHFAVPQAKGNNVVITSYMTNRGFYADKQSTFAPSFLLNIKGKKTSV
                     VKDSILEQGQLTVNK"
     CDS             2596..2613
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(2666..2684)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      2680..2727
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      2764..3219
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2851..2870)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3061..3082
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             complement(3311..4126)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(3311..4126)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     4034..4053
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     4118..4137
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      4248..4836
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4737..4756
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4990..5007
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    5022..5161
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(5087..5106)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5247..5269
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(5263..5454)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLQDHA
                     DELYRSCLARFGDDGENL"
     CDS             complement(6263..7345)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     7307..7326
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(7346..7423)
                     /gene="lacI"
                     /label=lacI promoter
     primer_bind     7629..7648
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     7732..7751
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        7732..7750
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    7751..7775
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
ORIGIN
        1 ctagaaataa ttttgtttaa ctttaagaag gagatataca tatgcaccat catcatcatc
       61 attctatggt cttcacactc gaagatttcg ttggggactg gcgacagaca gccggctaca
      121 acctggacca agtccttgaa cagggaggtg tgtccagttt gtttcagaat ctcggggtgt
      181 ccgtaactcc gatccaaagg attgtcctga gcggtgaaaa tgggctgaag atcgacatcc
      241 atgtcatcat cccgtatgaa ggtctgagcg gcgaccaaat gggccagatc gaaaaaattt
      301 ttaaggtggt gtaccctgtg gacgatcatc actttaaggt gatcctgcac tatggcacac
      361 tggtaatcga cggggttacg ccgaacatga tcgactattt cggacggccg tatgaaggca
      421 tcgccgtgtt cgacggcaaa aagatcactg taacagggac cctgtggaac ggcaacaaaa
      481 ttatcgacga gcgcctgatc aaccccgacg gctccctgct gttccgagta accatcaacg
      541 gagtgaccgg ctggcggctg tgcgaacgca ttctggcggg taccgagaac ttgtacttcc
      601 aatccatgga gaccgacgtc cacatatacc tgccgttcac tattatttag tgaaatgaga
      661 tattatgata ttttctgaat tgtgattaaa aaggcaactt tatgcccatg caacagaaac
      721 tataaaaaat acagagaatg aaaagaaaca gatagatttt ttagttcttt aggcccgtag
      781 tctgcaaatc cttttatgat tttctatcaa acaaaagagg aaaatagacc agttgcaatc
      841 caaacgagag tctaatagaa tgaggtcgaa aagtaaatcg cgcgggtttg ttactgataa
      901 agcaggcaag acctaaaatg tgtaaagggc aaagtgtata ctttggcgtc accccttaca
      961 tattttaggt ctttttttat tgtgcgtaac taacttgcca tcttcaaaca ggagggctgg
     1021 aagaagcaga ccgctaacac agtacataaa aaaggagaca tgaacgatga acatcaaaaa
     1081 gtttgcaaaa caagcaacag tattaacctt tactaccgca ctgctggcag gaggcgcaac
     1141 tcaagcgttt gcgaaagaaa cgaaccaaaa gccatataag gaaacatacg gcatttccca
     1201 tattacacgc catgatatgc tgcaaatccc tgaacagcaa aaaaatgaaa aatataaagt
     1261 tcctgagttc gattcgtcca caattaaaaa tatctcttct gcaaaaggcc tggacgtttg
     1321 ggacagctgg ccattacaaa acactgacgg cactgtcgca aactatcacg gctaccacat
     1381 cgtctttgca ttagccggag atcctaaaaa tgcggatgac acatcgattt acatgttcta
     1441 tcaaaaagtc ggcgaaactt ctattgacag ctggaaaaac gctggccgcg tctttaaaga
     1501 cagcgacaaa ttcgatgcaa atgattctat cctaaaagac caaacacaag aatggtcagg
     1561 ttcagccaca tttacatctg acggaaaaat ccgtttattc tacactgatt tctccggtaa
     1621 acattacggc aaacaaacac tgacaactgc acaagttaac gtatcagcat cagacagctc
     1681 tttgaacatc aacggtgtag aggattataa atcaatcttt gacggtgacg gaaaaacgta
     1741 tcaaaatgta cagcagttca tcgatgaagg caactacagc tcaggcgaca accatacgct
     1801 gagagatcct cactacgtag aagataaagg ccacaaatac ttagtatttg aagcaaacac
     1861 tggaactgaa gatggctacc aaggcgaaga atctttattt aacaaagcat actatggcaa
     1921 aagcacatca ttcttccgtc aagaaagtca aaaacttctg caaagcgata aaaaacgcac
     1981 ggctgagtta gcaaacggcg ctctcggtat gattgagcta aacgatgatt acacactgaa
     2041 aaaagtgatg aaaccgctga ttgcatctaa cacagtaaca gatgaaattg aacgcgcgaa
     2101 cgtctttaaa atgaacggca aatggtacct gttcactgac tcccgcggat caaaaatgac
     2161 gattgacggc attacgtcta acgatattta catgcttggt tatgtttcta attctttaac
     2221 tggcccatac aagccgctga acaaaactgg ccttgtgtta aaaatggatc ttgatcctaa
     2281 cgatgtaacc tttacttact cacacttcgc tgtacctcaa gcgaaaggaa acaatgtcgt
     2341 gattacaagc tatatgacaa acagaggatt ctacgcagac aaacaatcaa cgtttgcgcc
     2401 tagcttcctg ctgaacatca aaggcaagaa aacatctgtt gtcaaagaca gcatccttga
     2461 acaaggacaa ttaacagtta acaaataaaa acgcaaaaga aaatgccgat atcctattgg
     2521 cattgacggt ctccagtaaa ggtggatacg gatccgaatt cgagctccgt cgacaagctt
     2581 gcggccgcac tcgagcacca ccaccaccac cactgagatc cggctgctaa caaagcccga
     2641 aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc
     2701 tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg attggcgaat
     2761 gggacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga
     2821 ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg
     2881 ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat
     2941 ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg
     3001 ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata
     3061 gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt
     3121 tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat
     3181 ttaacgcgaa ttttaacaaa atattaacgc ttacaattta ggtggcactt ttcggggaaa
     3241 tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat
     3301 gaattaattc ttagaaaaac tcatcgagca tcaaatgaaa ctgcaattta ttcatatcag
     3361 gattatcaat accatatttt tgaaaaagcc gtttctgtaa tgaaggagaa aactcaccga
     3421 ggcagttcca taggatggca agatcctggt atcggtctgc gattccgact cgtccaacat
     3481 caatacaacc tattaatttc ccctcgtcaa aaataaggtt atcaagtgag aaatcaccat
     3541 gagtgacgac tgaatccggt gagaatggca aaagtttatg catttctttc cagacttgtt
     3601 caacaggcca gccattacgc tcgtcatcaa aatcactcgc atcaaccaaa ccgttattca
     3661 ttcgtgattg cgcctgagcg agacgaaata cgcgatcgct gttaaaagga caattacaaa
     3721 caggaatcga atgcaaccgg cgcaggaaca ctgccagcgc atcaacaata ttttcacctg
     3781 aatcaggata ttcttctaat acctggaatg ctgttttccc ggggatcgca gtggtgagta
     3841 accatgcatc atcaggagta cggataaaat gcttgatggt cggaagaggc ataaattccg
     3901 tcagccagtt tagtctgacc atctcatctg taacatcatt ggcaacgcta cctttgccat
     3961 gtttcagaaa caactctggc gcatcgggct tcccatacaa tcgatagatt gtcgcacctg
     4021 attgcccgac attatcgcga gcccatttat acccatataa atcagcatcc atgttggaat
     4081 ttaatcgcgg cctagagcaa gacgtttccc gttgaatatg gctcataaca ccccttgtat
     4141 tactgtttat gtaagcagac agttttattg ttcatgacca aaatccctta acgtgagttt
     4201 tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt
     4261 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt
     4321 ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag
     4381 ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta
     4441 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat
     4501 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg
     4561 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg
     4621 agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac
     4681 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga
     4741 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt
     4801 ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta
     4861 cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat
     4921 tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg
     4981 accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc
     5041 cttacgcatc tgtgcggtat ttcacaccgc aatggtgcac tctcagtaca atctgctctg
     5101 atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc
     5161 gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc
     5221 cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc
     5281 atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcct gaagcgattc
     5341 acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt
     5401 ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc
     5461 ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat
     5521 gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa
     5581 acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg
     5641 cttcgttaat acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat
     5701 ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa
     5761 accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca
     5821 cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag
     5881 ccgggtcctc aacgacagga gcacgatcat gcgcacccgt ggggccgcca tgccggcgat
     5941 aatggcctgc ttctcgccga aacgtttggt ggcgggacca gtgacgaagg cttgagcgag
     6001 ggcgtgcaag attccgaata ccgcaagcga caggccgatc atcgtcgcgc tccagcgaaa
     6061 gcggtcctcg ccgaaaatga cccagagcgc tgccggcacc tgtcctacga gttgcatgat
     6121 aaagaagaca gtcataagtg cggcgacgat agtcatgccc cgcgcccacc ggaaggagct
     6181 gactgggttg aaggctctca agggcatcgg tcgagatccc ggtgcctaat gagtgagcta
     6241 acttacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca
     6301 gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgccaggg
     6361 tggtttttct tttcaccagt gagacgggca acagctgatt gcccttcacc gcctggccct
     6421 gagagagttg cagcaagcgg tccacgctgg tttgccccag caggcgaaaa tcctgtttga
     6481 tggtggttaa cggcgggata taacatgagc tgtcttcggt atcgtcgtat cccactaccg
     6541 agatatccgc accaacgcgc agcccggact cggtaatggc gcgcattgcg cccagcgcca
     6601 tctgatcgtt ggcaaccagc atcgcagtgg gaacgatgcc ctcattcagc atttgcatgg
     6661 tttgttgaaa accggacatg gcactccagt cgccttcccg ttccgctatc ggctgaattt
     6721 gattgcgagt gagatattta tgccagccag ccagacgcag acgcgccgag acagaactta
     6781 atgggcccgc taacagcgcg atttgctggt gacccaatgc gaccagatgc tccacgccca
     6841 gtcgcgtacc gtcttcatgg gagaaaataa tactgttgat gggtgtctgg tcagagacat
     6901 caagaaataa cgccggaaca ttagtgcagg cagcttccac agcaatggca tcctggtcat
     6961 ccagcggata gttaatgatc agcccactga cgcgttgcgc gagaagattg tgcaccgccg
     7021 ctttacaggc ttcgacgccg cttcgttcta ccatcgacac caccacgctg gcacccagtt
     7081 gatcggcgcg agatttaatc gccgcgacaa tttgcgacgg cgcgtgcagg gccagactgg
     7141 aggtggcaac gccaatcagc aacgactgtt tgcccgccag ttgttgtgcc acgcggttgg
     7201 gaatgtaatt cagctccgcc atcgccgctt ccactttttc ccgcgttttc gcagaaacgt
     7261 ggctggcctg gttcaccacg cgggaaacgg tctgataaga gacaccggca tactctgcga
     7321 catcgtataa cgttactggt ttcacattca ccaccctgaa ttgactctct tccgggcgct
     7381 atcatgccat accgcgaaag gttttgcgcc attcgatggt gtccgggatc tcgacgctct
     7441 cccttatgcg actcctgcat taggaagcag cccagtagta ggttgaggcc gttgagcacc
     7501 gccgccgcaa ggaatggtgc atgcaaggag atggcgccca acagtccccc ggccacgggg
     7561 cctgccacca tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct
     7621 tccccatcgg tgatgtcggc gatataggcg ccagcaaccg cacctgtggc gccggtgatg
     7681 ccggccacga tgcgtccggc gtagaggatc gagatctcga tcccgcgaaa ttaatacgac
     7741 tcactatagg ggaattgtga gcggataaca attcccct
//
