LOCUS       sequence-346665-        6295 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Punc-25-mcherry::rab-3 Expresses Red-tagged synapses specifically in
            C. elegans GABAergic motor neurons.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6295)
  AUTHORS   Noma K, Goncharov A, Ellisman MH, Jin Y
  TITLE     Microtubule-dependent ribosome localization in C. elegans neurons.
  JOURNAL   Elife. 2017 Aug 2;6. pii: e26376. doi: 10.7554/eLife.26376.
  PUBMED    28767038
REFERENCE   2  (bases 1 to 6295)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2017-08-2"; volume: "6. pii: e26376. doi"; pages: " 
            10.7554/eLife.26376"
COMMENT     Sequence Label: pCZGY405
FEATURES             Location/Qualifiers
     source          1..6295
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..22
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(37..42,43..60,61..67)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    75..91
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     80..102
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     99..115
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     99..115
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     protein_bind    1453..1477
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(3032..3056)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(3979..4001)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     4101..4120
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(4198..4216)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4284..4388
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4389..4457,4458..5249)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4607..4626)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5420..6008
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5909..5928
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6162..6179
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg
       61 tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct atgaccatga
      121 ttacgccaag cttgatatcg aattccgttt ttttttttgg aaatatcgat tttttccgag
      181 atatctaaaa ttgaaagaaa ttttcgattt tctgagaaaa atacgaaaaa atcgaaaaaa
      241 aaacccaaat ttcggttttt tcgtaaaaaa agtcgtaaaa atgtaatttt tttcctgaaa
      301 aatcggaatt ttttacaaaa tatcggaaaa tactcaaaaa aaagctgaaa atttcgattt
      361 tccggagaaa aaatcttttt aaaaaaatat ttttttttca gaaaataaga aaagccgaaa
      421 tttataacta tttctccgga aattcgaaat ttttaacgaa atatcggaat aatttttaga
      481 tttttcaagt ttgactttgc gagaaaaaaa tcggaaaaac ctcgattcga cgccgaaaaa
      541 tgctcctttt cgaaaagatt tttgaaattt cagaaaatcg acatgcaagc gcgctccacg
      601 gcgaaatgac aacgatgatc caccgccctc aaaaagttgg gtctcgttag gtatttggcg
      661 gtaaaactgg taaaactcca gttttgcctc caacgagacc caattttttg gggcggtggt
      721 ggagcgcgct tgcacaagct gaaagcattt ttctgcgact cgataatatt ttgaaaacct
      781 gtgtcaattc tcgaaattct ttttttaaaa aataatcccg agcttctctc agtcctcctc
      841 tatgaggatg ttcctttttt ttggtttttc aatttttttt aaaattccaa atttctgttg
      901 tgcaattcac ttccccccaa gaaatcccaa aaatccccag ttttccccaa aaatgttccg
      961 ttttcatgtg atttttcccc catttttaaa acattttttt gacttttttt taaaatgatt
     1021 attattattg ttttctattt catggccggt aaattatttt ttttctttct ttttttttgc
     1081 tctttttttt caagaatttt cgaattgttt gaagggctgc tcatctaatc ttttgtcatt
     1141 ttgttctgat gccatcattt ctgagaggac ctttgaagac tcgtcacgaa acgggagggg
     1201 ggctcaagtg agcattatta ttattattat tgtcgcaaaa agtttacccc gggctccccc
     1261 tggctcccct ctttgagcaa gggtttaagg gctcattttg atgacgaatt gctcattggg
     1321 attatagtca cgcccctctt ttggagcaac tacacaactg agccacagta atccttgggg
     1381 gcggggtcag taggaccccc tccggaatag ggaaaagctc agttcaccgc caaagatccc
     1441 cgggattgga tcacaagttt gtacaaaaaa gcaggctccg aattcgccct tatggtctca
     1501 aagggtgaag aagataacat ggcaattatt aaagagttta tgcgtttcaa ggtgcatatg
     1561 gagggatctg tcaatgggca tgagtttgaa attgaaggtg aaggagaagg ccgaccatat
     1621 gagggaacac aaaccgcaaa actaaaggta agtttaaaca tatatatact aactaaccct
     1681 gattatttaa attttcaggt aactaaaggc ggaccattac cattcgcctg ggacatcctc
     1741 tctccacagt tcatgtatgg aagtaaagct tatgttaaac atccggcaga tataccagat
     1801 tatttgaaac tttcattccc ggagggtttt aagtgggaac gcgtaatgaa ttttgaagac
     1861 ggaggagttg ttacagtgac gcaagactca aggtaagttt aaacagttcg gtactaacta
     1921 accatacata tttaaatttt cagcctccaa gatggagaat ttatttataa agtcaaactt
     1981 cgaggaacga atttcccctc ggatggacct gttatgcaga agaagactat gggatgggaa
     2041 gcttcaagtg aaagaatgta ccctgaagac ggtgctctta agggagagat taaacaacgt
     2101 cttaaattga aagatggagg acattacgat gctgaggtaa gtttaaacat gattttacta
     2161 actaactaat ctgatttaaa ttttcaggtg aagacaactt acaaagccaa aaaaccagtt
     2221 cagctgccag gagcgtacaa tgttaatatt aaactggata tcacctccca caacgaggat
     2281 tacactatcg ttgagcaata tgaaagagct gaagggcggc actcgacagg tggcatggat
     2341 gaattgtata aggctagcat ggcggctggc ggacaacctc aaggcgctac accgggacaa
     2401 cccgatcaga actttaacta catgttcaag ctcctgataa tcggaaattc atcagttgga
     2461 aaaacatcat ccctcttccg ttactgtgat gattcattca cttctgcctt cgtctctact
     2521 gtcggaatcg atttcaaagt gaaaactgtg ttccgtggag acaaacgagt caaacttcaa
     2581 atctgggata ccgccggaca ggagaggtac cgtaccatca ccaccgccta ctatcgtgga
     2641 gcaatgggat tcattctgat gtatgacatc actaatgaag agtcttttaa tagtgttcag
     2701 gattggtgca ctcaaatcaa gacatactca tgggaaaatg ctcaagttgt tttggttgga
     2761 aataaatgtg atatggactc tgaaagagtt gtatctatgg ataggggacg ccaacttgct
     2821 gatcaacttg gtttggaatt cttcgaaaca tcagccaagg agaacattaa tgtaaaggca
     2881 gtttttgaga agttggtgga gattatttgt gataagatgg cagagagttt ggataaggac
     2941 ccacagcaac agccaaaagg acagaagctc ggagcgaatc cgacccaaaa gcctgctcaa
     3001 cagcaatgca attgctaaaa gggcgaattc gacccagctt tcttgtacaa agtggtgatc
     3061 caaaggaccc aaaggtatgt ttcgaatgat actaacataa catagaacat tttcaggagg
     3121 acccttggct agcgtcgacg gtaccatggt attgatatct gagctccgca tcggccgctg
     3181 tcatcagatc gccatctcgc gcccgtgcct ctgacttcta agtccaatta ctcttcaaca
     3241 tccctacatg ctctttctcc ctgtgctccc accccctatt tttgttatta tcaaaaaact
     3301 tctcttaatt tctttgtttt ttagcttctt ttaagtcacc tctaacaatg aaattgtgta
     3361 gattcaaaaa tagaattaat tcgtaataaa aagtcgaaaa aaattgtgct ccctcccccc
     3421 attaataata attctatccc aaaatctaca caatgttctg tgtacacttc ttatgttttt
     3481 tacttctgat aaattttttt gaaacatcat agaaaaaacc gcacacaaaa taccttatca
     3541 tatgttacgt ttcagtttat gaccgcaatt tttatttctt cgcacgtctg ggcctctcat
     3601 gacgtcaaat catgctcatc gtgaaaaagt tttggagtat ttttggaatt tttcaatcaa
     3661 gtgaaagttt atgaaattaa ttttcctgct tttgcttttt ggggtttccc ctattgtttg
     3721 tcaagatttc gaggacggcg tttttcttgc taaaatcaca agtattgatg agcacgatgc
     3781 aagaaagatc ggaagaaggt ttgggtttga ggctcagtgg aaggtgagta gaagttgata
     3841 atttgaaagt ggagtagtgt ctatggggtt tttgccttaa atgacagaat acattcccaa
     3901 tataccaaac ataactgttt ctactagtcg gccgtacggg ccctttcgtc tcgcgcgttt
     3961 cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca cagcttgtct
     4021 gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg
     4081 tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc accatatgcg
     4141 gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcggc cttaagggcc
     4201 tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag
     4261 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
     4321 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
     4381 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt
     4441 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt
     4501 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt
     4561 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg
     4621 tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga
     4681 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa
     4741 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga
     4801 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa
     4861 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca
     4921 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta
     4981 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac
     5041 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc
     5101 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag
     5161 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga
     5221 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt
     5281 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
     5341 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
     5401 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
     5461 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
     5521 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc
     5581 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
     5641 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
     5701 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
     5761 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
     5821 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
     5881 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
     5941 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
     6001 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
     6061 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
     6121 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
     6181 aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat
     6241 gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaat
//
