LOCUS       sequence-346667-        8797 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Pges-1 DAF-16d/f unc-54 3' UTR C.elegans gut expression of isoforms 
            d/f of DAF-16.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8797)
  AUTHORS   Hung WL, Wang Y, Chitturi J, Zhen M
  TITLE     A Caenorhabditis elegans developmental decision requires insulin 
            signaling-mediated neuron-intestine communication.
  JOURNAL   Development. 2014 Apr;141(8):1767-79. doi: 10.1242/dev.103846. Epub 
            2014 Mar 26.
  PUBMED    24671950
REFERENCE   2  (bases 1 to 8797)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1242/dev.103846"; journalName: "Development"; date: "2014-04"; 
            volume: "141"; issue: "8"; pages: "1767-79"
COMMENT     Sequence Label: pJH3377
FEATURES             Location/Qualifiers
     source          1..8797
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     133..152
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     386..403
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    520..541
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(556..561,562..579,580..586)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    594..610
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     599..621
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     618..634
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     618..634
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6339..6356)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6339..6355)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6348..6370)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6568..7023
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6655..6674)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6865..6886
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7040..7059)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7159..7181
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(7219..7237)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7305..7409
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7410..7478,7479..8270)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7628..7647)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      join(8441..8797,1..232)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga
       61 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
      121 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
      181 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
      241 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc
      301 tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc
      361 tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc
      421 aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag
      481 gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt agctcactca
      541 ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg gaattgtgag
      601 cggataacaa tttcacacag gaaacagcta tgacatgatt acgaattcga atggccatgg
      661 gacgtcgacg catagcccgc agtcacttga aagcacattt aagatggaat caaatggtaa
      721 gaaagctcgt gtaatcagga gattttatcc ggggtatgcc gaggaatata tggtgcactc
      781 gcattagctg gcattttttg taattatcgg atttctacct ttttcttaat ctagtttgtt
      841 atcattgtcc atcatttaca accaaaaatt tgcgtcattt acaaccaaaa attgatctga
      901 caattatcat aattcggttt ttattttcag aagaaacgct ccgatgtgat cctatagtga
      961 atatactttt ccatcctctc tgaaataaac aattatgaag aatgcatcaa tttgcattcg
     1021 aaataactgc cgtattttgt ctattggtat ggctgcagta ccatttttct tcgcgtcttt
     1081 gcgcagcctt cgggcgctac caataaggct aagccggggt aagttatttc agaattctaa
     1141 tcaactaatc taagtctcat acatcattgt caagtgacgc atttttttaa tttctatgtt
     1201 aactcgactt taatattctt ttctggtttc accagaaaaa tgctggactg aaaaattgaa
     1261 tttcccgcga tttgggaaaa accgcagttt aagcttttgg catgaataca gtgaacacag
     1321 gaacaaaaaa ttgacttgtt attttgagtt tatattaaat gttaattttg catctcatat
     1381 tttcacaaaa cttgtttgct tgaaaaatgt gcaaataaca agtgaacata catcaaggtt
     1441 tatgcagatc agcactgatg caactgatag caaaactgat aagggtcaaa atttcagtgg
     1501 ccagcacaaa cacatacaca ttcgtagttc gttttgtgtt tcgtgtattt agcatttatt
     1561 tttcaattcc ggcaatttgc cgatttgccg gaaattttga tttttggcaa attgccagtt
     1621 tgccgtttgc cgaacatcaa tttgccgaaa ttttttagag ggatctttat aagacggaaa
     1681 cacttaaaat tgtgcttttt tggaattttt ttcaatgttt caatagaatt agcttgcatt
     1741 taaaattaga tgtaggaacg tttataggac gcttacaatt ttgccgatta aaatcgaagt
     1801 tctgaacttt tcaaaaaatg tgcaaaattt tttttgacaa atttgacaaa tcggccggtt
     1861 tgtcgattgt ccggaatttt tcagtttcgg cgatttaccg gtttgccgga aaacatcgtt
     1921 tgccacccac acacacataa agagacgaaa caacacacca tcttcaaaaa atttgtgaat
     1981 cttcatttcc acgtggtttg cagggtttcc catccacccc actctatctc tccaagttga
     2041 attttggcaa aattgaccac acaccgatca aaaactattg tgtgtgtgtg tatttgtgtc
     2101 ttcaataatt cctgtctgtg tctcgcacta tctattgact acaagaacgt gatcgcagct
     2161 ctacgaaacc tttactttat tttagaaata ttttttcatg ctattagcaa aaaaaaagtt
     2221 tttttttgtc taagtacact taaaattttc caaaattttt aagatttttc aaaatgtgcg
     2281 atcaaagatc tgacaaaatg ccaaaatttt gaagaggaaa tttagtcggc ttccaaaatt
     2341 ataagtggca gaaattgagc aattgtcact ttttgacagt aaatagaaaa ttttttgaaa
     2401 aattttgaaa cgctttattt tgttacttgg tcattttggc accataggag tagtttttaa
     2461 cattttcccc actggcgcta cttcatttta aacttttcca aagattttaa tttttaattt
     2521 taaaaaattt catgtaattc gtctgcgtct tacagtttca ggcttcaaaa atctattaca
     2581 tatcttatct ttgaattcag gcttatgcaa gcgtggaact gtcgtgaggt atggcgccgc
     2641 atacgcaaac accttgcaca cacatcgaga actaccgtat actcgcagtt taaatttcaa
     2701 attttcgaaa tttttttcca atcatttttc cctatttttc ttcatttcgc tgctaaaaat
     2761 agttgttttg aacccgattt tgtgcgaaat cgtggaagtt tacacagctt ttacagttga
     2821 aaatcaaaaa agcgcgcttt tttcgtctaa tttgcccagt tttcagctcg attcgccgct
     2881 accatctgac atcacactgc acaatctcga accggcaagg cctgattccg gaatgagttt
     2941 ttccactgat tttgacggta aattcgtgat ttaaagttgt aaattccaat tttatttgtt
     3001 tttttttcag atgatttctt caatctcgac ctccatcaac aagagcgttc ggcttctttt
     3061 ggcggagtaa cccagtattc tcaacaattt cttcgcgaaa aatgctcgtt ctctccgtat
     3121 ttccacacat ctttagagac tgttgacagc ggaagaacta gcctatacgg gagcaatgag
     3181 caatgtggac agctcggcgg agcatcttca aacgggtcga cagcaatgct tcatactcca
     3241 gatggaagca attctcatca gacatcgttt ccttcggaaa tgtccgaatc gccagacgat
     3301 accgtatcgg gaaaaaagac aacgaccaga cggaacgctt ggggaaatat gtcatatgct
     3361 gaacttatca ctacagccat tatggctagt ccagagaaac ggttaactct tgcacaagtt
     3421 tacgaatgga tggtccagaa tgttccatac ttcagggata agggagattc gaacagttca
     3481 gctggatgga agaactcgat ccgtcacaat ctgtctcttc attctcgttt catgcgaatt
     3541 cagaatgaag gagccggaaa gagctcgtgg tgggttatta atccagatgc aaagccagga
     3601 aggaatccac ggcgtacacg tgaacgatcc aatactattg agacgactac aaaggctcaa
     3661 ctcgaaaaat ctcgccgcgg agccaagaag aggataaagg agagagcatt gatgggctcc
     3721 cttcactcga cacttaatgg aaattcgatt gccggatcga ttcaaacgat ttctcacgat
     3781 ttgtatgatg atgattcaat gcaaggagca tttgataacg ttccatcgtc tttccggtta
     3841 gtaatttagt tttattttta ttttttgaat tttccaaatt tccaaatttt ttcagtcccc
     3901 gaactcaatc gaacctctcg attcctggat cgtcgtctcg tgtttctcca gctattggaa
     3961 gtgatatcta tgatgatcta gaattcccat catcggttgg cgaatcggtt ccagcaattc
     4021 caagtgatat tgttgataga actgatcaaa tgcgtatcga tgcaactact catattggtg
     4081 gagttcagat taagcaggag tcgaagccga ttaaggtgag aaatgagaat ttgcaatttt
     4141 caggaaaatt tgaaaatttt cttgtggtta gtacaagtga gctccttttt agacaattaa
     4201 aaaatttgaa ttttacttaa attcaatatt tttaattctg aatttagttt caaggtcccc
     4261 taaaatatta gtaacatgca aatcatttta tattttctca caccgaaaat tagaattttc
     4321 ttttttttta agtgaaaaaa ctgagtaata tatagaattt tcagacggaa ccaattgctc
     4381 caccaccatc ataccacgag ttgaacagtg tccgtggatc gtgtgctcag aatccacttc
     4441 ttcgaaatcc aattgtgcca agcactaact tcaagccgtt agtttttttt tgaacagaag
     4501 ttaattaatc tatcgtaatt tcacaagatt ttgggtcccg gcgctcaaaa atgttttttt
     4561 taaattttta tgttaaaaat agttttaaat cagttttgaa agtgtaaatt ttggcttaaa
     4621 attattttaa acagaaaatt ggaaataaaa cgaagaaata aacttttttg cgcgccgaga
     4681 cccatctgaa taaatgcgtg cgcctttaag aaacctgtga aattactata tatacaaaat
     4741 tctgagaatg cgtattacgc aacaaatttg acgcgcaaaa tatctcgtag cgaaaactac
     4801 agtaatcctt aaaatgagta ctgtagcgct tgcgtcgatt tacgggctcg attttcgtga
     4861 attaatttat tttcgaaaag tgacagcgat attacattta actttgttct ttctaatcat
     4921 tttctcattt ttgtgaattt ttaaaaataa tttttcgatt aataaaggat tgccgtaaat
     4981 cgacacatgg tgctacagta ggcatttaaa ggattactgt agttttcgct acgagatatt
     5041 tcgcgcgtca aatatgttgc gtaatacgca ttctcagaat tttatgatcc cataatatat
     5101 atttcttctt taattcaata tataataatt atccaaaatt tgttcagaat gccactaccg
     5161 ggtgcctatg gaaactatca aaatggtgga ataactccaa tcaattggct atcaacatcc
     5221 agctcatctc cactgcctgg aattcaatcg tgtggaattg tagctgcaca gcatactgtc
     5281 gcttcttcat cggctcttcc aattgatttg gaaaatctga cacttcccga tcagccactg
     5341 atggatacta tggatgttga tgcattgatc agacatgagc tgagtcaagc tggagggcag
     5401 cagcatattc attttgattt gtaactgcag gaattcgata tgcagaagct gatctcagag
     5461 gaggacctgc ttcagaagct tatctcagag gaggaccttc gacctcgagg gggggcccgg
     5521 taccatggta ttgatatctg agctccgcat cggccgctgt catcagatcg ccatctcgcg
     5581 cccgtgcctc tgacttctaa gtccaattac tcttcaacat ccctacatgc tctttctccc
     5641 tgtgctccca ccccctattt ttgttattat caaaaaactt ctcttaattt ctttgttttt
     5701 tagcttcttt taagtcacct ctaacaatga aattgtgtag attcaaaaat agaattaatt
     5761 cgtaataaaa agtcgaaaaa aattgtgctc cctcccccca ttaataataa ttctatccca
     5821 aaatctacac aatgttctgt gtacacttct tatgtttttt acttctgata aatttttttg
     5881 aaacatcata gaaaaaaccg cacacaaaat accttatcat atgttacgtt tcagtttatg
     5941 accgcaattt ttatttcttc gcacgtctgg gcctctcatg acgtcaaatc atgctcatcg
     6001 tgaaaaagtt ttggagtatt tttggaattt ttcaatcaag tgaaagttta tgaaattaat
     6061 tttcctgctt ttgctttttg gggtttcccc tattgtttgt caagatttcg aggacggcgt
     6121 ttttcttgct aaaatcacaa gtattgatga gcacgatgca agaaagatcg gaagaaggtt
     6181 tgggtttgag gctcagtgga aggtgagtag aagttgataa tttgaaagtg gagtagtgtc
     6241 tatggggttt ttgccttaaa tgacagaata cattcccaat ataccaaaca taactgtttc
     6301 tactagtggc cacgtgggcc gtgcacctta agcttggcac tggccgtcgt tttacaacgt
     6361 cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca tccccctttc
     6421 gccagctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc
     6481 ctgaatggcg aatggcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca
     6541 caccgcatac gtcaaagcaa ccatagtacg cgccctgtag cggcgcatta agcgcggcgg
     6601 gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt
     6661 tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc
     6721 gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg
     6781 atttgggtga tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga
     6841 cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc
     6901 ctatctcggg ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa
     6961 aaaatgagct gatttaacaa aaatttaacg cgaattttaa caaaatatta acgtttacaa
     7021 ttttatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagccccgac
     7081 acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca
     7141 gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga
     7201 aacgcgcgag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa
     7261 taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt
     7321 gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa
     7381 tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta
     7441 ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag
     7501 taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca
     7561 gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta
     7621 aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc
     7681 gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc
     7741 ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca
     7801 ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc
     7861 acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca
     7921 taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac
     7981 tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg
     8041 cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg
     8101 ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg
     8161 gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac
     8221 gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc
     8281 aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct
     8341 aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc
     8401 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc
     8461 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg
     8521 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
     8581 atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
     8641 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
     8701 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa
     8761 cggggggttc gtgcacacag cccagcttgg agcgaac
//
