LOCUS       sequence-346668-        7230 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Pdpy-30 DAF-16a unc-54 3' UTR C.elegans pan-tissue expression of 
            isoform a of DAF-16 fused to GFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7230)
  AUTHORS   Hung WL, Wang Y, Chitturi J, Zhen M
  TITLE     A Caenorhabditis elegans developmental decision requires insulin 
            signaling-mediated neuron-intestine communication.
  JOURNAL   Development. 2014 Apr;141(8):1767-79. doi: 10.1242/dev.103846. Epub 
            2014 Mar 26.
  PUBMED    24671950
REFERENCE   2  (bases 1 to 7230)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1242/dev.103846"; journalName: "Development"; date: "2014-04"; 
            volume: "141"; issue: "8"; pages: "1767-79"
COMMENT     Sequence Label: pJH3043
FEATURES             Location/Qualifiers
     source          1..7230
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     116..135
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     369..386
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    503..524
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(539..544,545..562,563..569)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    577..593
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     582..604
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     601..617
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     601..617
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4755..4772)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4755..4771)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4764..4786)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4984..5439
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5071..5090)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5281..5302
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5456..5475)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5575..5597
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(5635..5653)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5721..5825
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5826..5894,5895..6686)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6044..6063)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      join(6857..7230,1..215)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca
       61 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
      121 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
      181 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac
      241 ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt
      301 ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga
      361 ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc aaaccgcctc
      421 tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag
      481 cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt
      541 tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca
      601 caggaaacag ctatgacatg attacgaatt cgaatggcca tgggacgtcg acgcggccgc
      661 tctagacacg cgtgtaacgt acaacataaa tgtttgacgt caaccagatg tagtacaata
      721 aaaaatatat tcagattacc tagaaaatta cttcggtcat gaccaccaat tttttgattt
      781 ttcgattttc gaaaccgaca tcgttgaaga acattcaggt cataattttt tatttgaaaa
      841 ataatgtttt ctatgtatta attcggttta gtcgatcgaa tatgtacagt tgagaattac
      901 tagagtacgg tgcgcaagta tgcaaacttg cgtaacggtg tttacacatt tgtcacgttt
      961 ctctgcgtct cttcgtctca acaatgcttg cactccgtca cactttcgaa ttgtcggccg
     1021 atatcggtat ttagataaat tcagtgctct cctttttttc tccattttac tttcatcaat
     1081 cttttttaaa aatcaatatt cagcaaacta ttgaaaaatt aattcaaaaa acacgactgg
     1141 tcggaagtta tttgcataat caatagaaat ttcgttttcc gctcgctgtc agtgttttgt
     1201 cctgtttgta tttcccagtg cacttttcga gtggctcaag tttttatcta ttttcctgca
     1261 aacttgcata tattgttttc ttccagaagc ctttgatatc tcaataattt tatacttgtt
     1321 tcagggatcc ctatgatgga gatgctggta gatcagggaa ctgatgcatc gtcatccgcc
     1381 tccacgtcca cctcatctgt ttcgagattc ggagcggaca cgttcatgaa tacaccggat
     1441 gatgtgatga tgaatgatga tatggaaccg attcctcgtg atcggtgcaa tacgtggcca
     1501 atgcgtaggc cgcaactcga accaccactc aactcgagtc ccattattca tgaacaaatt
     1561 cctgaagaag atgctgacct atacgggagc aatgagcaat gtggacagct cggcggagca
     1621 tcttcaaacg ggtcgacagc aatgcttcat actccagatg gaagcaattc tcatcagaca
     1681 tcgtttcctt cggaaatgtc cgaatcgcca gacgataccg tatcgggaaa aaagacaacg
     1741 accagacgga acgcttgggg aaatatgtca tatgctgaac ttatcactac agccattatg
     1801 gctagtccag agaaacggtt aactcttgca caagtttacg aatggatggt ccagaatgtt
     1861 ccatacttca gggataaggg agattcgaac agttcagctg gatggaagaa ctcgatccgt
     1921 cacaatctgt ctcttcattc tcgtttcatg cgaattcaga atgaaggagc cggaaagagc
     1981 tcgtggtggg ttattaatcc agatgcaaag ccaggaagga atccacggcg tacacgtgaa
     2041 cgatccaata ctattgagac gactacaaag gctcaactcg aaaaatctcg ccgcggagcc
     2101 aagaagagga taaaggagag agcattgatg ggctcccttc actcgacact taatggaaat
     2161 tcgattgccg gatcgattca aacgatttct cacgatttgt atgatgatga ttcaatgcaa
     2221 ggagcatttg ataacgttcc atcgtctttc cggttagtaa tttagtttta tttttatttt
     2281 ttgaattttc caaatttcca aattttttca gtccccgaac tcaatcgaac ctctcgattc
     2341 ctggatcgtc gtctcgtgtt tctccagcta ttggaagtga tatctatgat gatctagaat
     2401 tcccatcatc ggttggcgaa tcggttccag caattccaag tgatattgtt gatagaactg
     2461 atcaaatgcg tatcgatgca actactcata ttggtggagt tcagattaag caggagtcga
     2521 agccgattaa ggtgagaaat gagaatttgc aattttcagg aaaatttgaa aattttcttg
     2581 tggttagtac aagtgagctc ctttttagac aattaaaaaa tttgaatttt acttaaattc
     2641 aatattttta attctgaatt tagtttcaag gtcccctaaa atattagtaa catgcaaatc
     2701 attttatatt ttctcacacc gaaaattaga attttctttt tttttaagtg aaaaaactga
     2761 gtaatatata gaattttcag acggaaccaa ttgctccacc accatcatac cacgagttga
     2821 acagtgtccg tggatcgtgt gctcagaatc cacttcttcg aaatccaatt gtgccaagca
     2881 ctaacttcaa gccgttagtt tttttttgaa cagaagttaa ttaatctatc gtaatttcac
     2941 aagattttgg gtcccggcgc tcaaaaatgt tttttttaaa tttttatgtt aaaaatagtt
     3001 ttaaatcagt tttgaaagtg taaattttgg cttaaaatta ttttaaacag aaaattggaa
     3061 ataaaacgaa gaaataaact tttttgcgcg ccgagaccca tctgaataaa tgcgtgcgcc
     3121 tttaagaaac ctgtgaaatt actatatata caaaattctg agaatgcgta ttacgcaaca
     3181 aatttgacgc gcaaaatatc tcgtagcgaa aactacagta atccttaaaa tgagtactgt
     3241 agcgcttgcg tcgatttacg ggctcgattt tcgtgaatta atttattttc gaaaagtgac
     3301 agcgatatta catttaactt tgttctttct aatcattttc tcatttttgt gaatttttaa
     3361 aaataatttt tcgattaata aaggattgcc gtaaatcgac acatggtgct acagtaggca
     3421 tttaaaggat tactgtagtt ttcgctacga gatatttcgc gcgtcaaata tgttgcgtaa
     3481 tacgcattct cagaatttta tgatcccata atatatattt cttctttaat tcaatatata
     3541 ataattatcc aaaatttgtt cagaatgcca ctaccgggtg cctatggaaa ctatcaaaat
     3601 ggtggaataa ctccaatcaa ttggctatca acatccagct catctccact gcctggaatt
     3661 caatcgtgtg gaattgtagc tgcacagcat actgtcgctt cttcatcggc tcttccaatt
     3721 gatttggaaa atctgacact tcccgatcag ccactgatgg atactatgga tgttgatgca
     3781 ttgatcagac atgagctgag tcaagctgga gggcagcagc atattcattt tgatttgtaa
     3841 ctgcaggaat tcgatatgca gaagctgatc tcagaggagg acctgcttca gaagcttatc
     3901 tcagaggagg accttcgacc tcgagggggg gcccggtacc atggtattga tatctgagct
     3961 ccgcatcggc cgctgtcatc agatcgccat ctcgcgcccg tgcctctgac ttctaagtcc
     4021 aattactctt caacatccct acatgctctt tctccctgtg ctcccacccc ctatttttgt
     4081 tattatcaaa aaacttctct taatttcttt gttttttagc ttcttttaag tcacctctaa
     4141 caatgaaatt gtgtagattc aaaaatagaa ttaattcgta ataaaaagtc gaaaaaaatt
     4201 gtgctccctc cccccattaa taataattct atcccaaaat ctacacaatg ttctgtgtac
     4261 acttcttatg ttttttactt ctgataaatt tttttgaaac atcatagaaa aaaccgcaca
     4321 caaaatacct tatcatatgt tacgtttcag tttatgaccg caatttttat ttcttcgcac
     4381 gtctgggcct ctcatgacgt caaatcatgc tcatcgtgaa aaagttttgg agtatttttg
     4441 gaatttttca atcaagtgaa agtttatgaa attaattttc ctgcttttgc tttttggggt
     4501 ttcccctatt gtttgtcaag atttcgagga cggcgttttt cttgctaaaa tcacaagtat
     4561 tgatgagcac gatgcaagaa agatcggaag aaggtttggg tttgaggctc agtggaaggt
     4621 gagtagaagt tgataatttg aaagtggagt agtgtctatg gggtttttgc cttaaatgac
     4681 agaatacatt cccaatatac caaacataac tgtttctact agtggccacg tgggccgtgc
     4741 accttaagct tggcactggc cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt
     4801 acccaactta atcgccttgc agcacatccc cctttcgcca gctggcgtaa tagcgaagag
     4861 gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgaatg gcgcctgatg
     4921 cggtattttc tccttacgca tctgtgcggt atttcacacc gcatacgtca aagcaaccat
     4981 agtacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga
     5041 ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg
     5101 ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat
     5161 ttagtgcttt acggcacctc gaccccaaaa aacttgattt gggtgatggt tcacgtagtg
     5221 ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata
     5281 gtggactctt gttccaaact ggaacaacac tcaaccctat ctcgggctat tcttttgatt
     5341 tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat
     5401 ttaacgcgaa ttttaacaaa atattaacgt ttacaatttt atggtgcact ctcagtacaa
     5461 tctgctctga tgccgcatag ttaagccagc cccgacaccc gccaacaccc gctgacgcgc
     5521 cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga
     5581 gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgagacga aagggcctcg
     5641 tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg
     5701 gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa
     5761 atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga
     5821 agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc
     5881 ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg
     5941 gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc
     6001 gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat
     6061 tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg
     6121 acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag
     6181 aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa
     6241 cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc
     6301 gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca
     6361 cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc
     6421 tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc
     6481 tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg
     6541 ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta
     6601 tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag
     6661 gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga
     6721 ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc
     6781 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
     6841 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
     6901 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
     6961 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt
     7021 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
     7081 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
     7141 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
     7201 gcttggagcg aacgacctac accgaactga
//
