LOCUS       sequence-346669-        5474 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Punc-25-gfp1-10 Expresses split GFP parts 1-10 specifically in C. 
            elegans GABAergic motor neurons (only shows active fluorescence when
            binds to GFP11).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5474)
  AUTHORS   Noma K, Goncharov A, Ellisman MH, Jin Y
  TITLE     Microtubule-dependent ribosome localization in C. elegans neurons.
  JOURNAL   Elife. 2017 Aug 2;6. pii: e26376. doi: 10.7554/eLife.26376.
  PUBMED    28767038
REFERENCE   2  (bases 1 to 5474)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2017-08-2"; volume: "6. pii: e26376. doi"; pages: " 
            10.7554/eLife.26376"
COMMENT     Sequence Label: pCZGY3195
FEATURES             Location/Qualifiers
     source          1..5474
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..22
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(37..42,43..60,61..67)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    75..91
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     80..102
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     99..115
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     99..115
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     protein_bind    1453..1477
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      1498..1507
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(1549..1570)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     protein_bind    complement(2211..2235)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(3158..3180)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     3280..3299
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(3377..3395)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        3463..3567
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3568..3636,3637..4428)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3786..3805)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4599..5187
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5088..5107
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5341..5358
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg
       61 tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct atgaccatga
      121 ttacgccaag cttgatatcg aattccgttt ttttttttgg aaatatcgat tttttccgag
      181 atatctaaaa ttgaaagaaa ttttcgattt tctgagaaaa atacgaaaaa atcgaaaaaa
      241 aaacccaaat ttcggttttt tcgtaaaaaa agtcgtaaaa atgtaatttt tttcctgaaa
      301 aatcggaatt ttttacaaaa tatcggaaaa tactcaaaaa aaagctgaaa atttcgattt
      361 tccggagaaa aaatcttttt aaaaaaatat ttttttttca gaaaataaga aaagccgaaa
      421 tttataacta tttctccgga aattcgaaat ttttaacgaa atatcggaat aatttttaga
      481 tttttcaagt ttgactttgc gagaaaaaaa tcggaaaaac ctcgattcga cgccgaaaaa
      541 tgctcctttt cgaaaagatt tttgaaattt cagaaaatcg acatgcaagc gcgctccacg
      601 gcgaaatgac aacgatgatc caccgccctc aaaaagttgg gtctcgttag gtatttggcg
      661 gtaaaactgg taaaactcca gttttgcctc caacgagacc caattttttg gggcggtggt
      721 ggagcgcgct tgcacaagct gaaagcattt ttctgcgact cgataatatt ttgaaaacct
      781 gtgtcaattc tcgaaattct ttttttaaaa aataatcccg agcttctctc agtcctcctc
      841 tatgaggatg ttcctttttt ttggtttttc aatttttttt aaaattccaa atttctgttg
      901 tgcaattcac ttccccccaa gaaatcccaa aaatccccag ttttccccaa aaatgttccg
      961 ttttcatgtg atttttcccc catttttaaa acattttttt gacttttttt taaaatgatt
     1021 attattattg ttttctattt catggccggt aaattatttt ttttctttct ttttttttgc
     1081 tctttttttt caagaatttt cgaattgttt gaagggctgc tcatctaatc ttttgtcatt
     1141 ttgttctgat gccatcattt ctgagaggac ctttgaagac tcgtcacgaa acgggagggg
     1201 ggctcaagtg agcattatta ttattattat tgtcgcaaaa agtttacccc gggctccccc
     1261 tggctcccct ctttgagcaa gggtttaagg gctcattttg atgacgaatt gctcattggg
     1321 attatagtca cgcccctctt ttggagcaac tacacaactg agccacagta atccttgggg
     1381 gcggggtcag taggaccccc tccggaatag ggaaaagctc agttcaccgc caaagatccc
     1441 cgggattgga tcacaagttt gtacaaaaaa gcaggctccg aattcgccct tagatctgcc
     1501 accatggtga gcaagggcga ggagctgttc accggggtgg tgcccatcct ggtcgagctg
     1561 gacggcgacg taaacggcca caagttcagc gtgcgcggcg agggcgaggg cgatgccacc
     1621 attggcaagc tgaccctgaa gttcatctgc accaccggca agctgcccgt gccctggccc
     1681 accctcgtga ccaccctgac ctacggcgtg cagtgcttca gccgctaccc cgaccacatg
     1741 aagcagcacg acttcttcaa gtccgccatg cccgaaggct acgtccagga gcgcaccatc
     1801 agcttcaagg acgacggcaa atacaagacc cgcgccgtgg tgaagttcga gggcgacacc
     1861 ctggtgaacc gcatcgagct gaagggcacc gacttcaagg aggacggcaa catcctgggg
     1921 cacaagctgg agtacaactt taacagccac aacgtctata tcaccgccga caagcagaag
     1981 aacggcatca aggcgaactt caccgtgcgc cacaacgtgg aggacggcag cgtgcagctc
     2041 gccgaccact accagcagaa cacccccatc ggcgacggcc ccgtgctgct gcccgacaac
     2101 cactacctga gcacccagac cgtgctgagc aaagacccca acgagaagga gctcggatct
     2161 ggatctggat ctggatctgg atcttctgca ggctagcaag ggcgaattcg acccagcttt
     2221 cttgtacaaa gtggtgatcc aaaggaccca aaggtatgtt tcgaatgata ctaacataac
     2281 atagaacatt ttcaggagga cccttggcta gcgtcgacgg taccatggta ttgatatctg
     2341 agctccgcat cggccgctgt catcagatcg ccatctcgcg cccgtgcctc tgacttctaa
     2401 gtccaattac tcttcaacat ccctacatgc tctttctccc tgtgctccca ccccctattt
     2461 ttgttattat caaaaaactt ctcttaattt ctttgttttt tagcttcttt taagtcacct
     2521 ctaacaatga aattgtgtag attcaaaaat agaattaatt cgtaataaaa agtcgaaaaa
     2581 aattgtgctc cctcccccca ttaataataa ttctatccca aaatctacac aatgttctgt
     2641 gtacacttct tatgtttttt acttctgata aatttttttg aaacatcata gaaaaaaccg
     2701 cacacaaaat accttatcat atgttacgtt tcagtttatg accgcaattt ttatttcttc
     2761 gcacgtctgg gcctctcatg acgtcaaatc atgctcatcg tgaaaaagtt ttggagtatt
     2821 tttggaattt ttcaatcaag tgaaagttta tgaaattaat tttcctgctt ttgctttttg
     2881 gggtttcccc tattgtttgt caagatttcg aggacggcgt ttttcttgct aaaatcacaa
     2941 gtattgatga gcacgatgca agaaagatcg gaagaaggtt tgggtttgag gctcagtgga
     3001 aggtgagtag aagttgataa tttgaaagtg gagtagtgtc tatggggttt ttgccttaaa
     3061 tgacagaata cattcccaat ataccaaaca taactgtttc tactagtcgg ccgtacgggc
     3121 cctttcgtct cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg
     3181 agacggtcac agcttgtctg taagcggatg ccgggagcag acaagcccgt cagggcgcgt
     3241 cagcgggtgt tggcgggtgt cggggctggc ttaactatgc ggcatcagag cagattgtac
     3301 tgagagtgca ccatatgcgg tgtgaaatac cgcacagatg cgtaaggaga aaataccgca
     3361 tcaggcggcc ttaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata
     3421 atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt
     3481 ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg
     3541 cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt
     3601 cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta
     3661 aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc
     3721 ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa
     3781 gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc
     3841 cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt
     3901 acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact
     3961 gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac
     4021 aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata
     4081 ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta
     4141 ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg
     4201 gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat
     4261 aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt
     4321 aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga
     4381 aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa
     4441 gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag
     4501 gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac
     4561 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc
     4621 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat
     4681 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat
     4741 actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct
     4801 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt
     4861 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg
     4921 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta
     4981 cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg
     5041 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg
     5101 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc
     5161 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg
     5221 gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat
     5281 aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc
     5341 agcgagtcag tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg
     5401 cgttggccga ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt
     5461 gagcgcaacg caat
//
