LOCUS       sequence-346671-       10200 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Vector for overexpression of EGFP-tagged Stx17 with IRES for 
            hygromycin selection. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10200)
  AUTHORS   Janssen AFJ, Korsten G, Nijenhuis W, Katrukha EA, Kapitein LC
  TITLE     Direct observation of aggregate-triggered selective autophagy in 
            human cells.
  JOURNAL   J Cell Sci. 2021 Oct 1;134(19). pii: 271972. doi: 
            10.1242/jcs.258824. Epub 2021 Oct 6.
  PUBMED    34447998
REFERENCE   2  (bases 1 to 10200)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Sci."; date: "2021-10-1"; volume: "134(19). pii: 271972. doi"; 
            pages: " 10.1242/jcs.258824. Epub 2021 Oct 6"
COMMENT     Sequence Label: pLVX-IRES-hygro-EGFP-Stx17
FEATURES             Location/Qualifiers
     source          1..10200
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             17..650
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    697..822
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1319..1552
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1737..1781
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2044..2159
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     enhancer        2217..2520
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2521..2724
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2675..2695
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2721..2745
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      2825..2834
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(2831..2833,2834..2836,2837..3547)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(2831..2833,2834..2836,2837..3547)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2876..2897)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3137..3156)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3484..3505
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    join(4530..5104,5105..5107,5108..5116)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(4697..4714)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     4924..4943
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             5146..6165
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGYV
                     LRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPET
                     ELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQTV
                     MDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFGD
                     SQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGNF
                     DDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE"
     CDS             5146..6165
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGYV
                     LRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPET
                     ELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQTV
                     MDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFGD
                     SQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGNF
                     DDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE"
     misc_feature    6185..6773
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6238..6258)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6656..6667)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6980..7613
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(7741..7757)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7741..7757)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7754..7776)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7765..7781
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7789..7795,7796..7813,7814..7819))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7834..7855
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7972..7989)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8143..8731)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8223..8242)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8902..9693,9694..9762))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9525..9544
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9763..9867)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    9915..10049
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9952..9971)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10006..10025
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 ctacaaactt agtagttgga agggctaatt cactcccaaa gaagacaaga tatccttgat
       61 ctgtggatct accacacaca aggctacttc cctgattagc agaactacac accagggcca
      121 ggggtcagat atccactgac ctttggatgg tgctacaagc tagtaccagt tgagccagat
      181 aaggtagaag aggccaataa aggagagaac accagcttgt tacaccctgt gagcctgcat
      241 gggatggatg acccggagag agaagtgtta gagtggaggt ttgacagccg cctagcattt
      301 catcacgtgg cccgagagct gcatccggag tacttcaaga actgctgata tcgagcttgc
      361 tacaagggac tttccgctgg ggactttcca gggaggcgtg gcctgggcgg gactggggag
      421 tggcgagccc tcagatcctg catataagca gctgcttttt gcctgtactg ggtctctctg
      481 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
      541 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
      601 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg
      661 aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt
      721 gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg
      781 actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga
      841 attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta
      901 aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta
      961 gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga
     1021 tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg
     1081 atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt
     1141 aagaccaccg cacagcaagc ggccggccgc tgatcttcag acctggagga ggagatatga
     1201 gggacaattg gagaagtgaa ttatataaat ataaagtagt aaaaattgaa ccattaggag
     1261 tagcacccac caaggcaaag agaagagtgg tgcagagaga aaaaagagca gtgggaatag
     1321 gagctttgtt ccttgggttc ttgggagcag caggaagcac tatgggcgca gcgtcaatga
     1381 cgctgacggt acaggccaga caattattgt ctggtatagt gcagcagcag aacaatttgc
     1441 tgagggctat tgaggcgcaa cagcatctgt tgcaactcac agtctggggc atcaagcagc
     1501 tccaggcaag aatcctggct gtggaaagat acctaaagga tcaacagctc ctggggattt
     1561 ggggttgctc tggaaaactc atttgcacca ctgctgtgcc ttggaatgct agttggagta
     1621 ataaatctct ggaacagatt tggaatcaca cgacctggat ggagtgggac agagaaatta
     1681 acaattacac aagcttaata cactccttaa ttgaagaatc gcaaaaccag caagaaaaga
     1741 atgaacaaga attattggaa ttagataaat gggcaagttt gtggaattgg tttaacataa
     1801 caaattggct gtggtatata aaattattca taatgatagt aggaggcttg gtaggtttaa
     1861 gaatagtttt tgctgtactt tctatagtga atagagttag gcagggatat tcaccattat
     1921 cgtttcagac ccacctccca accccgaggg gacccgacag gcccgaagga atagaagaag
     1981 aaggtggaga gagagacaga gacagatcca ttcgattagt gaacggatct cgacggtatc
     2041 gcctttaaaa gaaaaggggg gattgggggg tacagtgcag gggaaagaat agtagacata
     2101 atagcaacag acatacaaac taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt
     2161 cgggtttatt acagggacag cagagatcca gtttatcgat aagcttggga gttccgcgtt
     2221 acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg cccattgacg
     2281 tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg acgtcaatgg
     2341 gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca tatgccaagt
     2401 acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc ccagtacatg
     2461 accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc tattaccatg
     2521 gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc acggggattt
     2581 ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac
     2641 tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg
     2701 tgggaggtct atataagcag agctcgttta gtgaaccgtc agatcgcctg gagacgccat
     2761 ccacgctgtt ttgacctcca tagaagacac cgactctact agaggatcta tttccggtga
     2821 attcgccacc atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt
     2881 cgagctggac ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg gcgagggcga
     2941 tgccacctac ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc
     3001 ctggcccacc ctcgtgacca ccctgaccta cggcgtgcag tgcttcagcc gctaccccga
     3061 ccacatgaag cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg
     3121 caccatcttc ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg
     3181 cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat
     3241 cctggggcac aagctggagt acaactacaa cagccacaac gtctatatca tggccgacaa
     3301 gcagaagaac ggcatcaagg tgaacttcaa gatccgccac aacatcgagg acggcagcgt
     3361 gcagctcgcc gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc
     3421 cgacaaccac tacctgagca cccagtccgc cctgagcaaa gaccccaacg agaagcgcga
     3481 tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc actctcggca tggacgagct
     3541 gtacaagggc ggatccggcg gagggagcgg agggggctca ctcgagtctg aagatgaaga
     3601 aaaagtgaaa ttacgccgtc ttgaaccagc tatccagaaa ttcattaaga tagtaatccc
     3661 aacagacctg gaaaggttaa gaaagcacca gataaatatt gagaagtatc aaaggtgcag
     3721 aatctgggac aagttgcatg aagagcatat caatgcagga cgtacagttc agcaactccg
     3781 atccaatatc cgagaaattg agaaactttg tttgaaagtc cgaaaggatg acctagtact
     3841 tctgaagaga atgatagatc ctgttaaaga agaagcatca gcagcaacag cagaatttct
     3901 ccaactccat ttggaatctg tagaagaact taagaagcaa tttaatgatg aagaaacttt
     3961 gctacagcct cctttgacca gatccatgac tgttggtgga gcatttcata ctactgaagc
     4021 tgaagctagt tctcagagtt tgactcagat atatgcctta cctgaaattc ctcaagatca
     4081 aaatgctgca gaatcgtggg aaaccttaga agcggactta attgaactta gccaactggt
     4141 cactgacttc tctctcctag tgaattctca gcaggagaag attgacagca ttgcagacca
     4201 tgtcaacagt gctgctgtga atgttgaaga gggaaccaaa aacttaggga aggctgcaaa
     4261 atacaagctg gcagctctgc ctgtggcagg tgcactcatc gggggaatgg tagggggtcc
     4321 tattggcctc cttgcaggct tcaaagtggc aggaattgca gctgcacttg gtggtggggt
     4381 gttgggcttc acaggtggaa aattgataca aagaaagaaa cagaaaatga tggagaagct
     4441 cacttccagc tgtccagatc ttcccagcca aactgacaag aaatgcagtt aaactagttc
     4501 tagagcggcc gcggatctgg ccaattccgc ccctctccct cccccccccc taacgttact
     4561 ggccgaagcc gcttggaata aggccggtgt gcgtttgtct atatgttatt ttccaccata
     4621 ttgccgtctt ttggcaatgt gagggcccgg aaacctggcc ctgtcttctt gacgagcatt
     4681 cctaggggtc tttcccctct cgccaaagga atgcaaggtc tgttgaatgt cgtgaaggaa
     4741 gcagttcctc tggaagcttc ttgaagacaa acaacgtctg tagcgaccct ttgcaggcag
     4801 cggaaccccc cacctggcga caggtgcctc tgcggccaaa agccacgtgt ataagataca
     4861 cctgcaaagg cggcacaacc ccagtgccac gttgtgagtt ggatagttgt ggaaagagtc
     4921 aaatggctct cctcaagcgt attcaacaag gggctgaagg atgcccagaa ggtaccccat
     4981 tgtatgggat ctgatctggg gcctcggtgc acatgcttta catgtgttta gtcgaggtta
     5041 aaaaaacgtc taggcccccc gaaccacggg gacgtggttt tcctttgaaa aacacgatga
     5101 taagcttgcc acaacccggg atcctctaga atggatagat ccggaaagcc tgaactcacc
     5161 gcgacgtctg tcgagaagtt tctgatcgaa aagttcgaca gcgtctccga cctgatgcag
     5221 ctctcggagg gcgaagaatc tcgtgctttc agcttcgatg taggagggcg tggatatgtc
     5281 ctgcgggtaa atagctgcgc cgatggtttc tacaaagatc gttatgttta tcggcacttt
     5341 gcatcggccg cgctcccgat tccggaagtg cttgacattg gggaatttag cgagagcctg
     5401 acctattgca tctcccgccg tgcacagggt gtcacgttgc aagacctgcc tgaaaccgaa
     5461 ctgcccgctg ttctgcagcc ggtcgcggag gccatggatg cgatcgctgc ggccgatctt
     5521 agccagacga gcgggttcgg cccattcgga ccgcaaggaa tcggtcaata cactacatgg
     5581 cgtgatttca tatgcgcgat tgctgatccc catgtgtatc actggcaaac tgtgatggac
     5641 gacaccgtca gtgcgtccgt cgcgcaggct ctcgatgagc tgatgctttg ggccgaggac
     5701 tgccccgaag tccggcacct cgtgcacgcg gatttcggct ccaacaatgt cctgacggac
     5761 aatggccgca taacagcggt cattgactgg agcgaggcga tgttcgggga ttcccaatac
     5821 gaggtcgcca acatcttctt ctggaggccg tggttggctt gtatggagca gcagacgcgc
     5881 tacttcgagc ggaggcatcc ggagcttgca ggatcgccgc ggctccgggc gtatatgctc
     5941 cgcattggtc ttgaccaact ctatcagagc ttggttgacg gcaatttcga tgatgcagct
     6001 tgggcgcagg gtcgatgcga cgcaatcgtc cgatccggag ccgggactgt cgggcgtaca
     6061 caaatcgccc gcagaagcgc ggccgtctgg accgatggct gtgtagaagt actcgccgat
     6121 agtggaaacc gacgccccag cactcgtccg agggcaaagg aataggtcga cacgcgtctg
     6181 gaacaatcaa cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt
     6241 tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc
     6301 ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga
     6361 gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc
     6421 cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct
     6481 ccctattgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg
     6541 gctgttgggc actgacaatt ccgtggtgtt gtcggggaag ctgacgtcct ttccatggct
     6601 gctcgcctgt gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc
     6661 cctcaatcca gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg
     6721 tcttcgcctt cgccctcaga cgagtcggat ctccctttgg gccgcctccc cgcctggaat
     6781 taattctgca gtcgagacct agaaaaacat ggagcaatca caagtagcaa tacagcagct
     6841 accaatgctg attgtgcctg gctagaagca caagaggagg aggaggtggg ttttccagtc
     6901 acacctcagg tacctttaag accaatgact tacaaggcag ctgtagatct tagccacttt
     6961 ttaaaagaaa agaggggact ggaagggcta attcactccc aacgaagaca agatatcctt
     7021 gatctgtgga tctaccacac acaaggctac ttccctgatt agcagaacta cacaccaggg
     7081 ccaggggtca gatatccact gacctttgga tggtgctaca agctagtacc agttgagcca
     7141 gataaggtag aagaggccaa taaaggagag aacaccagct tgttacaccc tgtgagcctg
     7201 catgggatgg atgacccgga gagagaagtg ttagagtgga ggtttgacag ccgcctagca
     7261 tttcatcacg tggcccgaga gctgcatccg gagtacttca agaactgctg atatcgagct
     7321 tgctacaagg gactttccgc tggggacttt ccagggaggc gtggcctggg cgggactggg
     7381 gagtggcgag ccctcagatc ctgcatataa gcagctgctt tttgcctgta ctgggtctct
     7441 ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa
     7501 gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc
     7561 tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtagtag
     7621 ttcatgtcat cttattattc agtatttata acttgcaaag aaatgaatat cagagagtga
     7681 gaggccttga cattgctagc gtttaccgtc gacctctagc tagagcttgg cgtaatcatg
     7741 gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc
     7801 cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc
     7861 gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat
     7921 cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac
     7981 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt
     8041 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
     8101 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
     8161 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
     8221 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
     8281 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
     8341 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
     8401 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
     8461 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
     8521 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
     8581 aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
     8641 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
     8701 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
     8761 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag
     8821 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata
     8881 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat
     8941 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg
     9001 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc
     9061 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc
     9121 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc
     9181 gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc
     9241 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc
     9301 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa
     9361 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat
     9421 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata
     9481 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca
     9541 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag
     9601 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc
     9661 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc
     9721 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata
     9781 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta
     9841 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcga
     9901 cggatcggga gatcaacttg tttattgcag cttataatgg ttacaaataa agcaatagca
     9961 tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac
    10021 tcatcaatgt atcttatcat gtctggatca actggataac tcaagctaac caaaatcatc
    10081 ccaaacttcc caccccatac cctattacca ctgccaatta cctgtggttt catttactct
    10141 aaacctgtga ttcctctgaa ttattttcat tttaaagaaa ttgtatttgt taaatatgta
//
