LOCUS       sequence-346672-       11625 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Vector for overexpression of EGFP-tagged DFCP1 with IRES for 
            hygromycin selection. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11625)
  AUTHORS   Janssen AFJ, Korsten G, Nijenhuis W, Katrukha EA, Kapitein LC
  TITLE     Direct observation of aggregate-triggered selective autophagy in 
            human cells.
  JOURNAL   J Cell Sci. 2021 Oct 1;134(19). pii: 271972. doi: 
            10.1242/jcs.258824. Epub 2021 Oct 6.
  PUBMED    34447998
REFERENCE   2  (bases 1 to 11625)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Sci."; date: "2021-10-1"; volume: "134(19). pii: 271972. doi"; 
            pages: " 10.1242/jcs.258824. Epub 2021 Oct 6"
COMMENT     Sequence Label: pLVX-IRES-hygro-EGFP-DFCP1
FEATURES             Location/Qualifiers
     source          1..11625
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             32..665
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    712..837
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1334..1567
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1752..1796
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2059..2174
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     enhancer        2232..2535
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2536..2739
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2690..2710
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2736..2760
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      2840..2849
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(2846..2848,2849..2851,2852..3562)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(2846..2848,2849..2851,2852..3562)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2891..2912)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3152..3171)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3499..3520
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    join(5970..6544,6545..6547,6548..6556)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(6137..6154)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     6364..6383
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             6586..7605
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGYV
                     LRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPET
                     ELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQTV
                     MDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFGD
                     SQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGNF
                     DDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE"
     CDS             6586..7605
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGYV
                     LRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPET
                     ELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQTV
                     MDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFGD
                     SQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGNF
                     DDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE"
     misc_feature    7625..8213
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7678..7698)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8096..8107)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8420..9053
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(9181..9197)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9181..9197)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9194..9216)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9205..9221
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9229..9235,9236..9253,9254..9259))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9274..9295
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9412..9429)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9583..10171)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9663..9682)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10342..11133,11134..11202))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10965..10984
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11203..11307)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    11355..11489
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(11392..11411)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     11446..11465
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tttgttaaat atgtactaca aacttagtag ttggaagggc taattcactc ccaaagaaga
       61 caagatatcc ttgatctgtg gatctaccac acacaaggct acttccctga ttagcagaac
      121 tacacaccag ggccaggggt cagatatcca ctgacctttg gatggtgcta caagctagta
      181 ccagttgagc cagataaggt agaagaggcc aataaaggag agaacaccag cttgttacac
      241 cctgtgagcc tgcatgggat ggatgacccg gagagagaag tgttagagtg gaggtttgac
      301 agccgcctag catttcatca cgtggcccga gagctgcatc cggagtactt caagaactgc
      361 tgatatcgag cttgctacaa gggactttcc gctggggact ttccagggag gcgtggcctg
      421 ggcgggactg gggagtggcg agccctcaga tcctgcatat aagcagctgc tttttgcctg
      481 tactgggtct ctctggttag accagatctg agcctgggag ctctctggct aactagggaa
      541 cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct
      601 gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc
      661 tagcagtggc gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga
      721 cgcaggactc ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt
      781 acgccaaaaa ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt
      841 attaagcggg ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag
      901 aaaaaatata aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt
      961 aatcctggcc tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca
     1021 tcccttcaga caggatcaga agaacttaga tcattatata atacagtagc aaccctctat
     1081 tgtgtgcatc aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa
     1141 gagcaaaaca aaagtaagac caccgcacag caagcggccg gccgctgatc ttcagacctg
     1201 gaggaggaga tatgagggac aattggagaa gtgaattata taaatataaa gtagtaaaaa
     1261 ttgaaccatt aggagtagca cccaccaagg caaagagaag agtggtgcag agagaaaaaa
     1321 gagcagtggg aataggagct ttgttccttg ggttcttggg agcagcagga agcactatgg
     1381 gcgcagcgtc aatgacgctg acggtacagg ccagacaatt attgtctggt atagtgcagc
     1441 agcagaacaa tttgctgagg gctattgagg cgcaacagca tctgttgcaa ctcacagtct
     1501 ggggcatcaa gcagctccag gcaagaatcc tggctgtgga aagataccta aaggatcaac
     1561 agctcctggg gatttggggt tgctctggaa aactcatttg caccactgct gtgccttgga
     1621 atgctagttg gagtaataaa tctctggaac agatttggaa tcacacgacc tggatggagt
     1681 gggacagaga aattaacaat tacacaagct taatacactc cttaattgaa gaatcgcaaa
     1741 accagcaaga aaagaatgaa caagaattat tggaattaga taaatgggca agtttgtgga
     1801 attggtttaa cataacaaat tggctgtggt atataaaatt attcataatg atagtaggag
     1861 gcttggtagg tttaagaata gtttttgctg tactttctat agtgaataga gttaggcagg
     1921 gatattcacc attatcgttt cagacccacc tcccaacccc gaggggaccc gacaggcccg
     1981 aaggaataga agaagaaggt ggagagagag acagagacag atccattcga ttagtgaacg
     2041 gatctcgacg gtatcgcctt taaaagaaaa ggggggattg gggggtacag tgcaggggaa
     2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca
     2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagttta tcgataagct
     2221 tgggagttcc gcgttacata acttacggta aatggcccgc ctggctgacc gcccaacgac
     2281 ccccgcccat tgacgtcaat aatgacgtat gttcccatag taacgccaat agggactttc
     2341 cattgacgtc aatgggtgga gtatttacgg taaactgccc acttggcagt acatcaagtg
     2401 tatcatatgc caagtacgcc ccctattgac gtcaatgacg gtaaatggcc cgcctggcat
     2461 tatgcccagt acatgacctt atgggacttt cctacttggc agtacatcta cgtattagtc
     2521 atcgctatta ccatggtgat gcggttttgg cagtacatca atgggcgtgg atagcggttt
     2581 gactcacggg gatttccaag tctccacccc attgacgtca atgggagttt gttttggcac
     2641 caaaatcaac gggactttcc aaaatgtcgt aacaactccg ccccattgac gcaaatgggc
     2701 ggtaggcgtg tacggtggga ggtctatata agcagagctc gtttagtgaa ccgtcagatc
     2761 gcctggagac gccatccacg ctgttttgac ctccatagaa gacaccgact ctactagagg
     2821 atctatttcc ggtgaattcg ccaccatggt gagcaagggc gaggagctgt tcaccggggt
     2881 ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc cacaagttca gcgtgtccgg
     2941 cgagggcgag ggcgatgcca cctacggcaa gctgaccctg aagttcatct gcaccaccgg
     3001 caagctgccc gtgccctggc ccaccctcgt gaccaccctg acctacggcg tgcagtgctt
     3061 cagccgctac cccgaccaca tgaagcagca cgacttcttc aagtccgcca tgcccgaagg
     3121 ctacgtccag gagcgcacca tcttcttcaa ggacgacggc aactacaaga cccgcgccga
     3181 ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa
     3241 ggaggacggc aacatcctgg ggcacaagct ggagtacaac tacaacagcc acaacgtcta
     3301 tatcatggcc gacaagcaga agaacggcat caaggtgaac ttcaagatcc gccacaacat
     3361 cgaggacggc agcgtgcagc tcgccgacca ctaccagcag aacaccccca tcggcgacgg
     3421 ccccgtgctg ctgcccgaca accactacct gagcacccag tccgccctga gcaaagaccc
     3481 caacgagaag cgcgatcaca tggtcctgct ggagttcgtg accgccgccg ggatcactct
     3541 cggcatggac gagctgtaca agggcggatc cggcggaggg agcggagggg gctcactcga
     3601 gagtgcccag acttccccag cagagaaggg cctgaatccg gggctgatgt gccaggaaag
     3661 ttacgcttgc agcgggactg atgaagctat ctttgagtgt gatgagtgct gcagtctgca
     3721 gtgtctccgc tgcgaggagg agctccatcg gcaggagcgc ctgagaaacc atgagcggat
     3781 aagactcaaa cctggccatg tcccttactg tgacctctgc aagggtctca gtgggcattt
     3841 accaggtgtt aggcagaggg caatagtgag gtgccagacc tgcaaaatta acttgtgcct
     3901 ggagtgccag aagaggactc attctggggg taacaaaagg agacaccctg ttactgtgta
     3961 caatgtcagt aatctccagg agtcactgga ggcagaagag atggatgagg agaccaagag
     4021 gaagaagatg actgagaagg ttgtgagttt cctcctagta gacgaaaatg aagaaattca
     4081 ggtaacaaat gaagaagact ttattagaaa attggactgc aaacctgatc agcatctgaa
     4141 agtggtttcc atttttggaa atactggtga tggaaagtct catactctca accacacttt
     4201 cttttatggt cgtgaagtct ttaaaacctc cccgacccag gagtcctgca ctgtgggagt
     4261 gtgggcagcc tatgacccag ttcacaaagt agcagtgatc gatacggaag ggctcctggg
     4321 ggccaccgtg aatctaagcc agagaacacg gctgctgctt aaggtcctgg ccatctcaga
     4381 cctcgtcatc tatcgaactc atgcagaccg gctgcataac gacctcttca aattccttgg
     4441 ggatgcctca gaagcttatc tgaagcactt caccaaggag ctcaaggcca ccactgctcg
     4501 ctgtggcctg gatgtccctt tatccacact gggccctgca gttatcatct tccatgagac
     4561 cgtgcacacc cagctactgg gctctgatca tccctcagag gtgccagaga agctcatcca
     4621 ggaccggttc cggaagctgg gccgtttccc tgaagccttt agttccattc actacaaggg
     4681 aacgaggact tacaaccctc ccacggactt ttctgggctt cggcgtgctt tggagcagct
     4741 actagagaat aacaccaccc gttctccccg gcacccggga gtcatcttca aagccctgaa
     4801 ggcactaagt gaccgcttca gcggtgagat ccccgatgac cagatggcgc acagctcctt
     4861 ttttccagat gagtatttca cctgctcctc cttgtgcctc agctgtgggg ttggatgtaa
     4921 gaaaagcatg aatcatggga aggaaggagt gcctcatgaa gccaagagcc gctgcagata
     4981 ctcccaccag tatgacaacc gagtgtatac ctgcaaggcc tgctatgaga gaggcgagga
     5041 agtcagtgta gtgcccaaaa catctgcttc cactgactcc ccctggatgg gtctcgcaaa
     5101 atatgcctgg tctgggtatg tgatcgaatg tcctaactgt ggcgtggtct atcgtagtcg
     5161 gcagtactgg tttggaaacc aagatcctgt ggatacggtg gtgcggacag agattgtgca
     5221 tgtgtggcct ggaactgatg ggtttctgaa ggacaacaac aatgctgccc agcgcctgtt
     5281 ggacgggatg aacttcatgg ctcagtcggt gtccgagctt agccttggac ccaccaaggc
     5341 tgtgacttcc tggctgacag accagatcgc ccctgcctac tggaggccca actcccagat
     5401 tctgagctgc aacaagtgtg cgacgtcctt taaagataac gacactaagc atcactgccg
     5461 agcctgtggg gagggcttct gtgacagctg ttcatcaaag actcggccag tgcctgagcg
     5521 gggctggggc cctgcgccag tgcgggtctg tgacaactgc tacgaagcca ggaacgtcca
     5581 gttagctgtt accgaggcac aagtggacga tgaaggtgga acgctcattg ctcggaaggt
     5641 gggcgaggcc gtgcagaaca ctctgggagc cgtggtgaca gccattgaca taccactagg
     5701 tctggtaaag gacgcggcca ggcctgcgta ctgggtgcct gaccacgaaa tcctccactg
     5761 ccacaactgc cggaaggagt tcagcatcaa gctctccaag caccactgcc gggcctgcgg
     5821 acagggcttc tgtgatgagt gctcccatga ccgccgggct gttccttctc gtggctggga
     5881 ccatcccgtc cgagtctgct tcaactgcaa taaaaagccc ggtgaccttt aaactagttc
     5941 tagagcggcc gcggatctgg ccaattccgc ccctctccct cccccccccc taacgttact
     6001 ggccgaagcc gcttggaata aggccggtgt gcgtttgtct atatgttatt ttccaccata
     6061 ttgccgtctt ttggcaatgt gagggcccgg aaacctggcc ctgtcttctt gacgagcatt
     6121 cctaggggtc tttcccctct cgccaaagga atgcaaggtc tgttgaatgt cgtgaaggaa
     6181 gcagttcctc tggaagcttc ttgaagacaa acaacgtctg tagcgaccct ttgcaggcag
     6241 cggaaccccc cacctggcga caggtgcctc tgcggccaaa agccacgtgt ataagataca
     6301 cctgcaaagg cggcacaacc ccagtgccac gttgtgagtt ggatagttgt ggaaagagtc
     6361 aaatggctct cctcaagcgt attcaacaag gggctgaagg atgcccagaa ggtaccccat
     6421 tgtatgggat ctgatctggg gcctcggtgc acatgcttta catgtgttta gtcgaggtta
     6481 aaaaaacgtc taggcccccc gaaccacggg gacgtggttt tcctttgaaa aacacgatga
     6541 taagcttgcc acaacccggg atcctctaga atggatagat ccggaaagcc tgaactcacc
     6601 gcgacgtctg tcgagaagtt tctgatcgaa aagttcgaca gcgtctccga cctgatgcag
     6661 ctctcggagg gcgaagaatc tcgtgctttc agcttcgatg taggagggcg tggatatgtc
     6721 ctgcgggtaa atagctgcgc cgatggtttc tacaaagatc gttatgttta tcggcacttt
     6781 gcatcggccg cgctcccgat tccggaagtg cttgacattg gggaatttag cgagagcctg
     6841 acctattgca tctcccgccg tgcacagggt gtcacgttgc aagacctgcc tgaaaccgaa
     6901 ctgcccgctg ttctgcagcc ggtcgcggag gccatggatg cgatcgctgc ggccgatctt
     6961 agccagacga gcgggttcgg cccattcgga ccgcaaggaa tcggtcaata cactacatgg
     7021 cgtgatttca tatgcgcgat tgctgatccc catgtgtatc actggcaaac tgtgatggac
     7081 gacaccgtca gtgcgtccgt cgcgcaggct ctcgatgagc tgatgctttg ggccgaggac
     7141 tgccccgaag tccggcacct cgtgcacgcg gatttcggct ccaacaatgt cctgacggac
     7201 aatggccgca taacagcggt cattgactgg agcgaggcga tgttcgggga ttcccaatac
     7261 gaggtcgcca acatcttctt ctggaggccg tggttggctt gtatggagca gcagacgcgc
     7321 tacttcgagc ggaggcatcc ggagcttgca ggatcgccgc ggctccgggc gtatatgctc
     7381 cgcattggtc ttgaccaact ctatcagagc ttggttgacg gcaatttcga tgatgcagct
     7441 tgggcgcagg gtcgatgcga cgcaatcgtc cgatccggag ccgggactgt cgggcgtaca
     7501 caaatcgccc gcagaagcgc ggccgtctgg accgatggct gtgtagaagt actcgccgat
     7561 agtggaaacc gacgccccag cactcgtccg agggcaaagg aataggtcga cacgcgtctg
     7621 gaacaatcaa cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt
     7681 tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc
     7741 ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga
     7801 gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc
     7861 cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct
     7921 ccctattgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg
     7981 gctgttgggc actgacaatt ccgtggtgtt gtcggggaag ctgacgtcct ttccatggct
     8041 gctcgcctgt gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc
     8101 cctcaatcca gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg
     8161 tcttcgcctt cgccctcaga cgagtcggat ctccctttgg gccgcctccc cgcctggaat
     8221 taattctgca gtcgagacct agaaaaacat ggagcaatca caagtagcaa tacagcagct
     8281 accaatgctg attgtgcctg gctagaagca caagaggagg aggaggtggg ttttccagtc
     8341 acacctcagg tacctttaag accaatgact tacaaggcag ctgtagatct tagccacttt
     8401 ttaaaagaaa agaggggact ggaagggcta attcactccc aacgaagaca agatatcctt
     8461 gatctgtgga tctaccacac acaaggctac ttccctgatt agcagaacta cacaccaggg
     8521 ccaggggtca gatatccact gacctttgga tggtgctaca agctagtacc agttgagcca
     8581 gataaggtag aagaggccaa taaaggagag aacaccagct tgttacaccc tgtgagcctg
     8641 catgggatgg atgacccgga gagagaagtg ttagagtgga ggtttgacag ccgcctagca
     8701 tttcatcacg tggcccgaga gctgcatccg gagtacttca agaactgctg atatcgagct
     8761 tgctacaagg gactttccgc tggggacttt ccagggaggc gtggcctggg cgggactggg
     8821 gagtggcgag ccctcagatc ctgcatataa gcagctgctt tttgcctgta ctgggtctct
     8881 ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa
     8941 gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc
     9001 tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtagtag
     9061 ttcatgtcat cttattattc agtatttata acttgcaaag aaatgaatat cagagagtga
     9121 gaggccttga cattgctagc gtttaccgtc gacctctagc tagagcttgg cgtaatcatg
     9181 gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc
     9241 cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc
     9301 gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat
     9361 cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac
     9421 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt
     9481 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
     9541 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
     9601 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
     9661 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
     9721 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
     9781 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
     9841 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
     9901 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
     9961 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
    10021 aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
    10081 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
    10141 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
    10201 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag
    10261 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata
    10321 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat
    10381 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg
    10441 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc
    10501 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc
    10561 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc
    10621 gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc
    10681 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc
    10741 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa
    10801 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat
    10861 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata
    10921 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca
    10981 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag
    11041 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc
    11101 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc
    11161 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata
    11221 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta
    11281 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcga
    11341 cggatcggga gatcaacttg tttattgcag cttataatgg ttacaaataa agcaatagca
    11401 tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac
    11461 tcatcaatgt atcttatcat gtctggatca actggataac tcaagctaac caaaatcatc
    11521 ccaaacttcc caccccatac cctattacca ctgccaatta cctgtggttt catttactct
    11581 aaacctgtga ttcctctgaa ttattttcat tttaaagaaa ttgta
//
