LOCUS       sequence-346673-        8804 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Low level expression of mKeima-PIM. Can be used for ratiometric 
            detection of inducible aggregate acidification after addition of 
            AP20187. mKeima fluorophore allows for simultaneous GFP-tagged 
            imaging..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8804)
  AUTHORS   Janssen AFJ, Korsten G, Nijenhuis W, Katrukha EA, Kapitein LC
  TITLE     Direct observation of aggregate-triggered selective autophagy in 
            human cells.
  JOURNAL   J Cell Sci. 2021 Oct 1;134(19). pii: 271972. doi: 
            10.1242/jcs.258824. Epub 2021 Oct 6.
  PUBMED    34447998
REFERENCE   2  (bases 1 to 8804)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Sci."; date: "2021-10-1"; volume: "134(19). pii: 271972. doi"; 
            pages: " 10.1242/jcs.258824. Epub 2021 Oct 6"
COMMENT     Sequence Label: pBA-mKeima-PIM
FEATURES             Location/Qualifiers
     source          1..8804
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     1065..1087
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     CDS             1166..1486
                     /codon_start=1
                     /product="F36V mutant of FK506-binding protein FKBP12"
                     /label=DmrB
                     /note="binds synthetic ligands that do not bind wild-type 
                     FKBP"
                     /translation="GVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKVDSSRDRNKP
                     FKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVELL
                     KLE"
     CDS             1493..1813
                     /codon_start=1
                     /product="F36V mutant of FK506-binding protein FKBP12"
                     /label=DmrB
                     /note="binds synthetic ligands that do not bind wild-type 
                     FKBP"
                     /translation="GVQVETISPGDGRTFPKRGQTCVEHYTGMLEDGKKVDSSRDRNKP
                     FKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDCAYGATGHPGIIPPHTTLVFDVELL
                     KLE"
     CDS             join(1829..1831,1832..2491)
                     /codon_start=1
                     /product="monomeric Keima-Red fluorescent protein, also 
                     known as mKeima"
                     /label=mKeima-Red
                     /translation="VSVIAKQMTYKVYMSGTVNGHYFEVEGDGKGKPYEGEQTVKLTVT
                     KGGPLPFAWDILSPQLQYGSIPFTKYPEDIPDYFKQSFPEGYTWERSMNFEDGAVCTVS
                     NDSSIQGNCFIYNVKISGENFPPNGPVMQKKTQGWEPSTERLFARDGMLIGNDYMALKL
                     EGGGHYLCEFKSTYKAKKPVRMPGRHEIDRKLDVTSHNRDYTSVEQCEIAIARHSLLG"
     CDS             3185..3505
                     /codon_start=1
                     /product="human FK506-binding protein FKBP12"
                     /label=FKBP
                     /translation="GVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKFDSSRDRNKP
                     FKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVELL
                     KLE"
     CDS             3512..3832
                     /codon_start=1
                     /product="human FK506-binding protein FKBP12"
                     /label=FKBP
                     /note="synthetic gene with alternative codons"
                     /translation="GVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKFDSSRDRNKP
                     FKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVELL
                     KLE"
     CDS             3512..3832
                     /codon_start=1
                     /product="human FK506-binding protein FKBP12"
                     /label=FKBP
                     /translation="GVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKFDSSRDRNKP
                     FKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVELL
                     KLE"
     polyA_signal    4124..4258
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4161..4180)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4215..4234
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(4272..4288)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4272..4288)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4285..4307)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4296..4312
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4320..4326,4327..4344,4345..4350))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4365..4386
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(4503..4520)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4674..5262)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4754..4773)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5433..6224,6225..6293))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6056..6075
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6294..6398)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     6466..6484
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(6522..6544)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     6644..6663
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6857..6879
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     6871..6888
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     6872..6888
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 ggggcggggc gaggcggaga ggtgcggcgg cagccaatca gagcggcgcg ctccgaaagt
       61 ttccttttat ggcgaggcgg cggcggcggc ggccctataa aaagcgaagc gcgcggcggg
      121 cgggagtcgc tgcgcgctgc cttcgccccg tgccccgctc cgccgccgcc tcgcgccgcc
      181 cgccccggct ctgactgacc gcgttactcc cacaggtgag cgggcgggac ggcccttctc
      241 ctccgggctg taattagcgc ttggtttaat gacggctcgt ttcttttctg tggctgcgtg
      301 aaagccttaa agggctccgg gagggccctt tgtgcggggg ggagcggctc ggggggtgcg
      361 tgcgtgtgtg tgtgcgtggg gagcgccgcg tgcggctccg cgctgcccgg cggctgtgag
      421 cgctgcgggc gcggcgcggg gctttgtgcg ctccgcagtg tgcgcgaggg gagcgcggcc
      481 gggggcggtg ccccgcggtg cggggggggc tgcgagggga acaaaggctg cgtgcggggt
      541 gtgtgcgtgg gggggtgagc agggggtgtg ggcgcggcgg tcgggctgta acccccccct
      601 gcacccccct ccccgagttg ctgagcacgg cccggcttcg ggtgcggggc tccgtgcggg
      661 gcgtggcgcg gggctcgccg tgccgggcgg ggggtggcgg caggtggggg tgccgggcgg
      721 ggcggggccg cctcgggccg gggagggctc gggggagggg cgcggcggcc cccggagcgc
      781 cggcggctgt cgaggcgcgg cgagccgcag ccattgcctt ttatggtaat cgtgcgagag
      841 ggcgcaggga cttcctttgt cccaaatctg tgcggagccg aaatctggga ggcgccgccg
      901 caccccctct agcgggcgcg gggcgaagcg gtgcggcgcc ggcaggaagg aaatgggcgg
      961 ggagggcctt cgtgcgtcgc cgcgccgccg tccccttctc catctccagc ctcggggctg
     1021 tccgcagggg gacggctgcc ttcggggggg acggggcagg gcggggttcg gcttctggcg
     1081 tgtgaccggc ggggtttata tcttcccttc tctgttcctc cgcagccccc aagctagcgc
     1141 taagcttgcc accatgcgct ctagaggcgt ccaagtcgaa accattagtc ccggcgatgg
     1201 cagaacattt cctaaaaggg gacaaacatg tgtcgtccat tatacaggca tgttggagga
     1261 cggcaaaaag gtggacagta gtagagatcg caataaacct ttcaaattca tgttgggaaa
     1321 acaagaagtc attaggggat gggaggaggg cgtggctcaa atgtccgtcg gccaacgcgc
     1381 taagctcacc atcagccccg actacgcata cggcgctacc ggacatcccg gaattattcc
     1441 ccctcacgct accttggtgt ttgacgtcga actgttgaag ctcgagacta gaggagtgca
     1501 ggtggagact atctccccag gagacgggcg caccttcccc aagcgcggcc agacctgcgt
     1561 ggagcactac accgggatgc ttgaagatgg aaagaaagtt gattcctccc gggacagaaa
     1621 caagcccttt aagtttatgc taggcaagca ggaggtgatc cgaggctggg aagaaggggt
     1681 tgcccagatg agtgtgggtc agagagccaa actgactata tctccagatt gtgcctatgg
     1741 tgccactggg cacccaggca tcatcccacc acataccact ctcgtcttcg atgtggagct
     1801 tctaaaactg gaaactagtg aaccggtagt gagtgtgatc gctaaacaaa tgacctacaa
     1861 ggtttatatg tcaggcacgg tcaatggaca ctactttgag gtcgaaggcg atggaaaagg
     1921 aaagccttac gagggagagc agacagtaaa gctcactgtc accaagggtg gacctctgcc
     1981 atttgcttgg gatattttat caccacagct tcagtacgga agcataccat tcaccaagta
     2041 ccctgaagac atccctgatt atttcaagca gtcattccct gagggatata catgggagag
     2101 gagcatgaac tttgaagatg gtgcagtgtg tactgtcagc aatgattcca gcatccaagg
     2161 caactgtttc atctacaatg tcaaaatctc tggtgagaac tttcctccca atggacctgt
     2221 tatgcagaag aagacacagg gctgggaacc cagcactgag cgtctctttg cacgagatgg
     2281 aatgctgata ggaaacgatt atatggctct gaagttggaa ggaggtggtc actatttgtg
     2341 tgaatttaaa tctacttaca aggcaaagaa gcctgtgagg atgccagggc gccacgagat
     2401 tgaccgcaaa ctggatgtaa ccagtcacaa cagggattac acatctgttg agcagtgtga
     2461 aatagccatt gcacgccact ctttgctcgg tggcgcgcct cgctcacgcg gcgttcaggt
     2521 ggagactata agcccggggg atggtcgcac ttttcctaaa cgaggccaga cgtgcgtagt
     2581 tcactatacc ggcatgcttg aagacggcaa gaaggtggat agctctaggg accggaataa
     2641 gcccttcaag ttcatgcttg gtaagcaaga agtgatccgg ggttgggaag agggggttgc
     2701 ccaaatgtca gtcggacaac gggcgaagtt gactatttct cctgattacg cttatggtgc
     2761 gacgggacat cctggcatca taccgcctca cgccactctt gtctttgatg tggaactttt
     2821 gaagctggaa acgcgagggg ttcaagttga gaccatttca ccgggcgatg gcagaacatt
     2881 cccgaaaagg ggacaaacgt gtgtagtaca ctatacgggc atgctcgaag acgggaaaaa
     2941 ggttgactca agtagagatc ggaacaagcc tttcaagttc atgttgggga aacaagaggt
     3001 aatacgcggg tgggaggagg gagtcgctca gatgtctgta ggccaaaggg cgaagctcac
     3061 catatcacca gattacgctt acggggcgac gggccatccc ggaataatac ctccgcacgc
     3121 gacacttgtt tttgatgttg aattgcttaa gttggagacc agcagatctg tcgaccgctc
     3181 tagaggagtg caggtggaaa ccatctcccc aggagacggg cgcaccttcc ccaagcgcgg
     3241 ccagacctgc gtggtgcact acaccgggat gcttgaagat ggaaagaaat ttgactcctc
     3301 ccgggacaga aacaagccct ttaagtttat gctaggcaag caggaggtga tccgaggctg
     3361 ggaagaaggg gttgcccaga tgagtgtggg tcagagagcc aaactgacta tatctccaga
     3421 ttatgcctat ggtgccactg ggcacccagg catcatccca ccacatgcca ctctcgtctt
     3481 cgatgtggag cttctaaaac tggaaactag aggcgtgcag gtcgagacca tcagccccgg
     3541 cgacggccgc acctttccca agagaggcca gacttgcgtg gtccactaca ccggcatgct
     3601 ggaggacggc aagaagttcg acagcagccg cgaccgcaac aagcccttca agttcatgct
     3661 gggcaaacag gaagtgatcc gcggctggga ggaaggcgtg gctcagatga gcgtggggca
     3721 gcgggccaag ctgaccatca gccccgacta tgcctacggc gccaccggcc accccggcat
     3781 catccccccc cacgccaccc tggtgttcga cgtggagctg ctgaagctgg agactagtta
     3841 ggaattcagg atccagtcga ctagtacttc tcgagctctg tacatgtccg cggtcgcgac
     3901 gtacgcgtat cgatggcgcc agctgcaggc ggccgccata tgcatcctag gcctattaat
     3961 attccggagt atacgtagcc ggctaacgtt aacaaccggt acctctagat aagtaatgat
     4021 cataatcagc catatcacat ctgtagaggt tttacttgct ttaaaaaacc tcccacacct
     4081 ccccctgaac ctgaaacata aaatgaatgc aattgttgtt gttaacttgt ttattgcagc
     4141 ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc
     4201 actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggatcaa
     4261 ttcgtaatca tgtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac
     4321 aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc
     4381 acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg
     4441 cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct
     4501 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac
     4561 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga
     4621 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
     4681 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
     4741 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
     4801 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
     4861 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
     4921 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
     4981 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
     5041 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
     5101 ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
     5161 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
     5221 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
     5281 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga
     5341 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc
     5401 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct
     5461 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata
     5521 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca
     5581 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga
     5641 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga
     5701 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg
     5761 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga
     5821 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt
     5881 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct
     5941 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca
     6001 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat
     6061 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga
     6121 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc
     6181 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg
     6241 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc
     6301 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt
     6361 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca
     6421 cctgacgtct aagaaaccat tattatcatg acattaacct ataaaaatag gcgtatcacg
     6481 aggccctttc gtctcgcgcg tttcggtgat gacggtgaaa acctctgaca catgcagctc
     6541 ccggagacgg tcacagcttg tctgtaagcg gatgccggga gcagacaagc ccgtcagggc
     6601 gcgtcagcgg gtgttggcgg gtgtcggggc tggcttaact atgcggcatc agagcagatt
     6661 gtactgagag tgcaccatat gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac
     6721 cgcatcaggc gccattcgcc attcaggctg cgcaactgtt gggaagggcg atcggtgcgg
     6781 gcctcttcgc tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg attaagttgg
     6841 gtaacgccag ggttttccca gtcacgacgt tgtaaaacga cggccagtgc caagttggga
     6901 tctttgcatt ggcccacggc tctcaggatg gggatgctcc ccttcagcac ccggttcccc
     6961 ttggaaactg atggtcctgg ctctgtggca tggcagtggc actgtgagga gccccctacc
     7021 agcagcacac agtggggttg gcactgccac gctccggatg ccgcgctctg atccaacccc
     7081 ataatcaagg gaacccgaat tgccccatca ttgcccccac cacccccatc ctgccgggcc
     7141 ctcacacccc acgctgcctt ctggtgacat tccccagccc aaacccacgg cttcatggct
     7201 accgcggggc atttcccatt gccgccccat tatcagctct gcacacctcc cgctgtaccc
     7261 atgcctcgtg gctgcccttc tttgacgtat aatcttctaa ttaatacccg gccttgtcaa
     7321 agtggagcac aaacgttaat taattcccca gcaggcaggt aattaacagt gtgactccct
     7381 ttttgctgcg agtggggctg atacagagag atgtggcact atggagccca cggggtcctg
     7441 gcactgggtg cccacggagg tccccatgtg ctgcagtgtc accgcctccg aggagacagt
     7501 attgtccctg cggtgtccct gcagctcagc tctgtccaca gggccacctc cagtttggag
     7561 gggacacaat gcagccccga tgcaacccat cctcgcagca tcccagggac aaagacccca
     7621 ctgcaagacc gcacacaggg ctgggtcccg ctcccctaat atctacagtg cttcttgcat
     7681 ggccccttaa tcaatgcagt taatcagcat gcgctcatgc accgctctgg agctgcaaag
     7741 cccctcgcag cgctgctcac caacaccgcg caccgccccg gcccagcctg cagcacgcgc
     7801 tgcaaacagg aaagaaacaa aatattgccc aaatgtaggc aaaggcattc ggctgccttg
     7861 acctccgccg ggccgggccc tgcctgactc agctccttac tcagcgctcg cttcctccct
     7921 ccggctgcca ccgccgcagc gcacaccctg acaaagagtg gcccttaacg ggctctgagg
     7981 tgcacccagc agtgcactca gcagtccaag ggccggcctg gaggtttgca ccgctacgtg
     8041 ctgacattag cattgaactt ggccctgggt agtgctgcag gccgggcggg gtgggtgtag
     8101 agagtgcagc gcgcgttgca cccggtgccc cttcccctcc cttgcatccc agcaggctgc
     8161 accccagcac caggcccgtg catgcatgct cctggtgtta ttgcagcctg gtgcatgcat
     8221 gcgtcttgat ggtgcagcgc tgtgcatgca tcctccttgg tgtgtagcag tctagtgcat
     8281 gcatacccct cggtgttatt gctgctctgt gcacgcacgc tcattgtatc acttcatccc
     8341 agtgcatgca ctcacactgg agcgattgct gctcggtgca cgcacactca ttgtatcact
     8401 gcagctcagt ggctgcacgc acaccggtgt tattgctgct cggtgcgtgc atgcacatca
     8461 gtgtcgctgc agctcagtgc atgcacgctc attgcccatc gctatccctg cctctcctgc
     8521 tggcgctccc cgggaggtga cttcaagggg accgcaggac cacctcgggg gtggggggag
     8581 ggctgcacac gcggaccccg ctccccctcc ccaacaaagc actgtggaat caaaaagggg
     8641 ggagggggga tggaggggcg cgtcacaccc ccgccccaca ccctcacctc gaggtgagcc
     8701 ccacgttctg cttcactctc cccatctccc ccccctcccc acccccaatt ttgtatttat
     8761 ttatttttta attattttgt gcagcgatgg gggcgggggg gggg
//
