LOCUS       sequence-346674-        7716 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Prgef-1-gfp1-10 Expresses split GFP parts 1-10 in all C. elegans 
            neurons (only shows active fluorescence when binds to GFP11).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7716)
  AUTHORS   Noma K, Goncharov A, Ellisman MH, Jin Y
  TITLE     Microtubule-dependent ribosome localization in C. elegans neurons.
  JOURNAL   Elife. 2017 Aug 2;6. pii: e26376. doi: 10.7554/eLife.26376.
  PUBMED    28767038
REFERENCE   2  (bases 1 to 7716)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2017-08-2"; volume: "6. pii: e26376. doi"; pages: " 
            10.7554/eLife.26376"
COMMENT     Sequence Label: pCZGY3196
FEATURES             Location/Qualifiers
     source          1..7716
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..22
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(37..42,43..60,61..67)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    75..91
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     80..102
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     99..115
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     99..115
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     protein_bind    3770..3794
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      3815..3824
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(3866..3887)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     protein_bind    complement(4534..4558)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(5400..5422)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     5522..5541
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(5619..5637)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5705..5809
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5810..5878,5879..6670)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6028..6047)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6841..7429
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7330..7349
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7583..7600
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg
       61 tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct atgaccatga
      121 ttacgccaag cttgcatgcc tgcaggtcga ctctagcccc cacacacatc gctgccggaa
      181 tagatgatcc catcgtagtt cgataatctc gtgacactcg tttccgatac ccccttatat
      241 cagcacacat tcagtcatca taccacaaaa tgttgtgata gtgataggca aaactaccgt
      301 atttcctcta ttaaccttgc atgcaagact aattttcgat taacccgtag gggtgcaaga
      361 ctaatagaga ctgcaagact attagaggct gaaatactaa ttttcgtatg ctcaataatt
      421 ttggaaattg gcctattttt tgtagaaact tgataccgtt taaacaagga aaaatacaca
      481 ctttttaata tttcatcaat aatttgaacg attttgtgat tttaagttca atttgcccaa
      541 aaaaagacaa tttttctgac gttaccgcaa agtaatgcag gtcaggcaaa aagagcggtg
      601 caaaaatata agagactgca atactaatag aggaaatacg gtaattgaat tttagtgaaa
      661 cttgcgacag ttttcctcat ttttttgtta ttccggcata cgagtgtggc atacgagtga
      721 ggtcatcttt tgtttcttcc gtttcttcat cgcttttgaa aaaaaatgtt gaagaaaact
      781 tcgtagccga tagccggata atgtttggca cggcgttcca acatatatca ttggaaattt
      841 tttaagattt ttgcgaaaaa tcacattctt cacgatgaga acacgttatt gaaggaatat
      901 aaatcaagaa taacatatag ttatatttct ctattacttt aacgttaaat atgagcaaat
      961 ttgagcattt tgattgcgat gaaaagcaga atcgagtcaa ctgaaatccg ttcaaaaacc
     1021 taagctcggt tcgcccgaaa gtcgattttt ccaatagccg aacagcactg cttctttatt
     1081 ttccaggtat ttttggggtg taagaattat atcttctttg tttgggtatt tacgtttccg
     1141 aattcccttt cttgatctgt cgctattttt cagaaaccag aacagtttat cctttttcat
     1201 gagaacctga atagctcaaa aacccgttta atttcttttc atcatctcat ttgaaacttt
     1261 cctagtcttc ttaatgattt cctggctcct cctacatttg tatctcaaga tgcatacact
     1321 ttatttccgc tccaattcga atcatctact tctctttttt cttcaacttt ctactttttt
     1381 ccattctttt ctttcaaatg ttcgacgtct tcaagtgact gctcttcatt ctcctcattt
     1441 ttctcgaatt tcattcttgt ctcttttttg ctcaaaaatg gaaaatgaaa gtgaagtgag
     1501 atttggacgg cgggacacgg gggcagtaga agcagcaaaa aggagagaaa gaggacacaa
     1561 ataagaagaa cgaattcaaa aataagcgga gaggagctat ttccgtcaat tctacctccc
     1621 caatcttcat caattcggct caattgaatg acgtcacagg gagataaacc gtttggatga
     1681 gcgccgtcca tcactgacgc catcccgttt gggacaagaa aagagaaaaa agagcacaaa
     1741 gtttttggtg acggatcttg tcaatcatat gaaagttgtt ctgattgatt gtcagttttt
     1801 ttcctacttt tttggattct atccacttct gaacttgtga caagtttcaa actttctgaa
     1861 tcattttcta tgcattttcc tggaattctt tttatgtaaa atatgaaata gaatgttttt
     1921 gaattcaagt tctgcttttt tcttcttttt tgttctgttc gggcttgggt atgctttttt
     1981 taaaaattat tttgcacatc gaccaataag tgcgcaactt ataaaattaa tttatttttg
     2041 ttaatttttg aaatacttgt attgctttaa gtgatctgac ctcgccctga gctttccacg
     2101 tagttatcaa atacaaatcc tccaagggta acgtacctat attactgatc tttataataa
     2161 ctttatcacc tgtccagttc cagaggaatt ctgttaagct tataggcaca gaaggagtca
     2221 tttctgctgg tttttaatga tccaaatctt tatttcaagt aaaaaactga acacttgcga
     2281 ataaaactat cagattaacc attcaccaaa aatgtgtttg aatctaaaac tttctcagta
     2341 ttccaaatat agaaataaat aaccacgaca ttgctaaaat ctgtctgaat tgtgtactcc
     2401 ttaccgtgaa gtaataatgg atataatgaa tcgtttgaaa tgaatgatca gcacattttt
     2461 ggtgaaagat cacaaataag gaataagcga cggaaaataa aacgattatt tcggatcaaa
     2521 aatttgttga agatcatata cactcgagac caagattatt ctagacaatt ttcaaattgg
     2581 cttctttgtt tgcaaatctt cataataatc tgtgaagttt ggaaatttga atttttaatc
     2641 ttttttcata gattatagtt tttatttctt tgcaaaacta tattaaaaac cgatgcattg
     2701 ttttagggaa attaatgagc cttttgttca acactaaaaa caataaaact agaattttgg
     2761 cttcatcatt tgaccttttt taagttcgaa aacttttctc gtatttctga accgccaatt
     2821 ttttcacaca tctctagact tttggtgccc gttccagaaa gttaagtaat tgctattcta
     2881 agaaagttct caacattgtt ttttagttct gattgaattc tgatgttcca ggaatatatt
     2941 ttaaatttaa tattctttgc actatttcta tactaactaa ataataaata gtctaagtat
     3001 gttcaatgag aacaaaaaag ctctatctat atttttctat cctatttcat ttatatcctt
     3061 ttcattttga actcaccagc tcctcttctt cttcttgtcc atatacttct taaaagtctc
     3121 gaacttctct ctctcactct ccatcaattt ctttatggct accgcttgcg ctctgccgct
     3181 tccgaagaaa gaaggcgttg gcagagctct caattctatt ttttctcgcc gtaggctatc
     3241 gatttctcag cttctcttct ctcttcctca ctccaccacc accacttcgg gcttcttctt
     3301 ttcttcattt tcgtcttctt cttcatcctc ttcttttttt ttcagttccc cctcactcct
     3361 ccccttatat gcgcgtgcga gaggggtgca aaagcagcgc gcatccgaga attgaaacga
     3421 gaaaacggag acgcagcagc agttcgtcct cagaaaaata gccagtaaaa agagaaaaag
     3481 atagagagag acctatcgca ttttattttc aaattgcaat tcctgcaatt catgtgtgcg
     3541 tgtgcccatt ttcaattctt cccgttattt ttcagaccat taccaacgtt ctttctagat
     3601 cttgagacaa tcttccttct gcagaatcaa gggcgaattc cagcacactg gcggccgtta
     3661 ctagaggatc cccgggattg gccaaaggac ccaaaggtat gtttcgaatg atactaacat
     3721 aacatagaac attttcagga ggacccttgg ctagcgtcga cggtacatca caagtttgta
     3781 caaaaaagca ggctccgaat tcgcccttag atctgccacc atggtgagca agggcgagga
     3841 gctgttcacc ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa acggccacaa
     3901 gttcagcgtg cgcggcgagg gcgagggcga tgccaccatt ggcaagctga ccctgaagtt
     3961 catctgcacc accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca ccctgaccta
     4021 cggcgtgcag tgcttcagcc gctaccccga ccacatgaag cagcacgact tcttcaagtc
     4081 cgccatgccc gaaggctacg tccaggagcg caccatcagc ttcaaggacg acggcaaata
     4141 caagacccgc gccgtggtga agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa
     4201 gggcaccgac ttcaaggagg acggcaacat cctggggcac aagctggagt acaactttaa
     4261 cagccacaac gtctatatca ccgccgacaa gcagaagaac ggcatcaagg cgaacttcac
     4321 cgtgcgccac aacgtggagg acggcagcgt gcagctcgcc gaccactacc agcagaacac
     4381 ccccatcggc gacggccccg tgctgctgcc cgacaaccac tacctgagca cccagaccgt
     4441 gctgagcaaa gaccccaacg agaaggagct cggatctgga tctggatctg gatctggatc
     4501 tggatcttct gcaggctagc aagggcgaat tcgacccagc tttcttgtac aaagtggtga
     4561 tgtaccatgg tattgatatc tgagctccgc atcggccgct gtcatcagat cgccatctcg
     4621 cgcccgtgcc tctgacttct aagtccaatt actcttcaac atccctacat gctctttctc
     4681 cctgtgctcc caccccctat ttttgttatt atcaaaaaac ttctcttaat ttctttgttt
     4741 tttagcttct tttaagtcac ctctaacaat gaaattgtgt agattcaaaa atagaattaa
     4801 ttcgtaataa aaagtcgaaa aaaattgtgc tccctccccc cattaataat aattctatcc
     4861 caaaatctac acaatgttct gtgtacactt cttatgtttt ttacttctga taaatttttt
     4921 tgaaacatca tagaaaaaac cgcacacaaa ataccttatc atatgttacg tttcagttta
     4981 tgaccgcaat ttttatttct tcgcacgtct gggcctctca tgacgtcaaa tcatgctcat
     5041 cgtgaaaaag ttttggagta tttttggaat ttttcaatca agtgaaagtt tatgaaatta
     5101 attttcctgc ttttgctttt tggggtttcc cctattgttt gtcaagattt cgaggacggc
     5161 gtttttcttg ctaaaatcac aagtattgat gagcacgatg caagaaagat cggaagaagg
     5221 tttgggtttg aggctcagtg gaaggtgagt agaagttgat aatttgaaag tggagtagtg
     5281 tctatggggt ttttgcctta aatgacagaa tacattccca atataccaaa cataactgtt
     5341 tctactagtc ggccgtacgg gccctttcgt ctcgcgcgtt tcggtgatga cggtgaaaac
     5401 ctctgacaca tgcagctccc ggagacggtc acagcttgtc tgtaagcgga tgccgggagc
     5461 agacaagccc gtcagggcgc gtcagcgggt gttggcgggt gtcggggctg gcttaactat
     5521 gcggcatcag agcagattgt actgagagtg caccatatgc ggtgtgaaat accgcacaga
     5581 tgcgtaagga gaaaataccg catcaggcgg ccttaagggc ctcgtgatac gcctattttt
     5641 ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa
     5701 tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat
     5761 gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca
     5821 acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca
     5881 cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta
     5941 catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt
     6001 tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc
     6061 cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc
     6121 accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc
     6181 cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa
     6241 ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga
     6301 accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat
     6361 ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca
     6421 attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc
     6481 ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat
     6541 tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag
     6601 tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa
     6661 gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca
     6721 tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc
     6781 ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc
     6841 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
     6901 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
     6961 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
     7021 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
     7081 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
     7141 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
     7201 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
     7261 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
     7321 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
     7381 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
     7441 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
     7501 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
     7561 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
     7621 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
     7681 tcccgactgg aaagcgggca gtgagcgcaa cgcaat
//
