LOCUS       sequence-346678-        7243 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses Myc Mutant (delta leucine zipper) in Mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7243)
  AUTHORS   Tu WB, Shiah YJ, Lourenco C, Mullen PJ, Dingar D, Redel C, Tamachi 
            A, Ba-Alawi W, Aman A, Al-Awar R, Cescon DW, Haibe-Kains B, 
            Arrowsmith CH, Raught B, Boutros PC, Penn LZ
  TITLE     MYC Interacts with the G9a Histone Methyltransferase to Drive 
            Transcriptional Repression and Tumorigenesis.
  JOURNAL   Cancer Cell. 2018 Oct 8;34(4):579-595.e8. doi: 
            10.1016/j.ccell.2018.09.001.
  PUBMED    30300580
REFERENCE   2  (bases 1 to 7243)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ccell.2018.09"; journalName: "Cancer Cell"; date: 
            "2018-10-8- 8"; volume: "34"; issue: "4"; pages: "579-595.e8"
COMMENT     Sequence Label: pcDNA3.1 MYC dLZ
FEATURES             Location/Qualifiers
     source          1..7243
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     8..28
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     102..121
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        102..120
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1377..1403
                     /codon_start=1
                     /product="nuclear localization signal of human c-Myc 
                     proto-oncogene (Dang and Lee, 1988)"
                     /label=c-myc NLS
                     /translation="PAAKRVKLD"
     promoter        complement(2035..2053)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(2036..2053)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(2073..2090)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    2079..2303
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      2349..2777
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2436..2455)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2646..2667
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(2786..2806)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        2791..3120
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      2971..3106
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     3033..3052
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             3187..3981
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3241..3260)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     3851..3870
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    4155..4276
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4192..4211)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4246..4265
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(4325..4341)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4325..4341)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4338..4360)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4349..4365
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4373..4379,4380..4397,4398..4403))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4418..4439
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(4556..4573)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4727..5315)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4807..4826)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5486..6277,6278..6346))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6109..6128
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6347..6451)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(6526..6545)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        6717..7096
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        join(7097..7243,1..57)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
ORIGIN
        1 ccattgacgc aaatgggcgg taggcgtgta cggtgggagg tctatataag cagagctctc
       61 tggctaacta gagaacccac tgcttactgg cttatcgaaa ttaatacgac tcactatagg
      121 gagacccaag cttggtaccg agctcggatc cactagtaac ggccgccagt gtgctggaat
      181 tcggcccccc cccccttgcc gcatccacga aactttgccc atagcagcgg gcgggcactt
      241 tgcactggaa cttacaacac ccgagcaagg acgcgactct cccgacgcgg ggaggctatt
      301 ctgcccattt ggggacactt ccccgccgct gccaggaccc gcttctctga aaggctctcc
      361 ttgcagctgc ttagacgctg gatttttttc gggtagtgga aaaccagcct cccgcgacga
      421 tgcccctcaa cgttagcttc accaacagga actatgacct cgactacgac tcggtgcagc
      481 cgtatttcta ctgcgacgag gaggagaact tctaccagca gcagcagcag agcgagctgc
      541 agcccccggc gcccagcgag gatatctgga agaaattcga gctgctgccc accccgcccc
      601 tgtcccctag ccgccgctcc gggctctgct cgccctccta cgttgcggtc acacccttct
      661 cccttcgggg agacaacgac ggcggtggcg ggagcttctc cacggccgac cagctggaga
      721 tggtgaccga gctgctggga ggagacatgg tgaaccagag tttcatctgc gacccggacg
      781 acgagacctt catcaaaaac atcatcatcc aggactgtat gtggagcggc ttctcggccg
      841 ccgccaagct cgtctcagag aagctggcct cctaccaggc tgcgcgcaaa gacagcggca
      901 gcccgaaccc cgcccgcggc cacagcgtct gctccacctc cagcttgtac ctgcaggatc
      961 tgagcgccgc cgcctcagag tgcatcgacc cctcggtggt cttcccctac cctctcaacg
     1021 acagcagctc gcccaagtcc tgcgcctcgc aagactccag cgccttctct ccgtcctcgg
     1081 attctctgct ctcctcgacg gagtcctccc cgcagggcag ccccgagccc ctggtgctcc
     1141 atgaggagac accgcccacc accagcagcg actctgagga ggaacaagaa gatgaggaag
     1201 aaatcgatgt tgtttctgtg gaaaagaggc aggctcctgg caaaaggtca gagtctggat
     1261 caccttctgc tggaggccac agcaaacctc ctcacagccc actggtcctc aagaggtgcc
     1321 acgtctccac acatcagcac aactacgcag cgcctccctc cactcggaag gactatcctg
     1381 ctgccaagag ggtcaagttg gacagtgtca gagtcctgag acagatcagc aacaaccgaa
     1441 aatgcaccag ccccaggtcc tcggacaccg aggagaatgt caagaggcga acacacaacg
     1501 tcttggagcg ccagaggagg aacgagctaa aacggagctt ttttgccctg cgtgaccaga
     1561 tcccggagtt ggaaaacaat gaaaaggccc ccaaggtagt tatccttaaa aaagccacag
     1621 catacatcct gtccgtccaa gcagaggagc aaaagctcct acggaactct tgtgcgtaag
     1681 gaaaagtaag gaaaatgatt ccttctaaca gaaatgtcct gagcaatcac ctatgaactt
     1741 gtttcaaatg catgatcaaa tgcaacctca caaccttggc tgagtcttga gactgaaaga
     1801 tttagccata atgtaaactg cctcaaattg gactttgggc ataaaagaac ttttttatgc
     1861 ttaccatctt ttttttttct ttaacagatt tgtatttaag aattgttttt aaaaaatttt
     1921 aagatttaca caatgtttct ctgtaaatat tgccattaaa tgtaaataac ttccgaattc
     1981 tgcagatatc catcacactg gcggccgctc gagcatgcat ctagagggcc ctattctata
     2041 gtgtcaccta aatgctagag ctcgctgatc agcctcgact gtgccttcta gttgccagcc
     2101 atctgttgtt tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt
     2161 cctttcctaa taaaatgagg aaattgcatc gcattgtctg agtaggtgtc attctattct
     2221 ggggggtggg gtggggcagg acagcaaggg ggaggattgg gaagacaata gcaggcatgc
     2281 tggggatgcg gtgggctcta tggcttctga ggcggaaaga accagctggg gctctagggg
     2341 gtatccccac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag
     2401 cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt
     2461 tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt
     2521 ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg
     2581 tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt
     2641 taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt
     2701 tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca
     2761 aaaatttaac gcgaattaat tctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca
     2821 ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc aaccaggtgt
     2881 ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca
     2941 gcaaccatag tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc
     3001 cattctccgc cccatggctg actaattttt tttatttatg cagaggccga ggccgcctct
     3061 gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa
     3121 aagctcccgg gagcttgtat atccattttc ggatctgatc aagagacagg atgaggatcg
     3181 tttcgcatga ttgaacaaga tggattgcac gcaggttctc cggccgcttg ggtggagagg
     3241 ctattcggct atgactgggc acaacagaca atcggctgct ctgatgccgc cgtgttccgg
     3301 ctgtcagcgc aggggcgccc ggttcttttt gtcaagaccg acctgtccgg tgccctgaat
     3361 gaactgcagg acgaggcagc gcggctatcg tggctggcca cgacgggcgt tccttgcgca
     3421 gctgtgctcg acgttgtcac tgaagcggga agggactggc tgctattggg cgaagtgccg
     3481 gggcaggatc tcctgtcatc tcaccttgct cctgccgaga aagtatccat catggctgat
     3541 gcaatgcggc ggctgcatac gcttgatccg gctacctgcc cattcgacca ccaagcgaaa
     3601 catcgcatcg agcgagcacg tactcggatg gaagccggtc ttgtcgatca ggatgatctg
     3661 gacgaagagc atcaggggct cgcgccagcc gaactgttcg ccaggctcaa ggcgcgcatg
     3721 cccgacggcg aggatctcgt cgtgacccat ggcgatgcct gcttgccgaa tatcatggtg
     3781 gaaaatggcc gcttttctgg attcatcgac tgtggccggc tgggtgtggc ggaccgctat
     3841 caggacatag cgttggctac ccgtgatatt gctgaagagc ttggcggcga atgggctgac
     3901 cgcttcctcg tgctttacgg tatcgccgct cccgattcgc agcgcatcgc cttctatcgc
     3961 cttcttgacg agttcttctg agcgggactc tggggttcga aatgaccgac caagcgacgc
     4021 ccaacctgcc atcacgagat ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg
     4081 gaatcgtttt ccgggacgcc ggctggatga tcctccagcg cggggatctc atgctggagt
     4141 tcttcgccca ccccaacttg tttattgcag cttataatgg ttacaaataa agcaatagca
     4201 tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac
     4261 tcatcaatgt atcttatcat gtctgtatac cgtcgacctc tagctagagc ttggcgtaat
     4321 catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac
     4381 gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa
     4441 ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat
     4501 gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc
     4561 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg
     4621 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag
     4681 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc
     4741 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
     4801 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
     4861 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
     4921 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
     4981 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
     5041 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
     5101 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
     5161 ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
     5221 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca
     5281 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg
     5341 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa
     5401 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta
     5461 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag
     5521 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga
     5581 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac
     5641 cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc
     5701 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta
     5761 gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac
     5821 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat
     5881 gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa
     5941 gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg
     6001 tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag
     6061 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc
     6121 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct
     6181 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat
     6241 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg
     6301 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc
     6361 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta
     6421 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg
     6481 tcgacggatc gggagatctc ccgatcccct atggtgcact ctcagtacaa tctgctctga
     6541 tgccgcatag ttaagccagt atctgctccc tgcttgtgtg ttggaggtcg ctgagtagtg
     6601 cgcgagcaaa atttaagcta caacaaggca aggcttgacc gacaattgca tgaagaatct
     6661 gcttagggtt aggcgttttg cgctgcttcg cgatgtacgg gccagatata cgcgttgaca
     6721 ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc atagcccata
     6781 tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac cgcccaacga
     6841 cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt
     6901 ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag tacatcaagt
     6961 gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca
     7021 ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct acgtattagt
     7081 catcgctatt accatggtga tgcggttttg gcagtacatc aatgggcgtg gatagcggtt
     7141 tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt tgttttggca
     7201 ccaaaatcaa cgggactttc caaaatgtcg taacaactcc gcc
//
