LOCUS       sequence-346681-       14639 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  For making Rabies virus SAD B19 strain L cell line with hyPBase.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14639)
  AUTHORS   Jin L, Sullivan HA, Zhu M, Lavin TK, Matsuyama M, Fu X, Lea NE, Xu 
            R, Hou Y, Rutigliani L, Pruner M, Babcock KR, Ip JPK, Hu M, Daigle 
            TL, Zeng H, Sur M, Feng G, Wickersham IR
  TITLE     Long-term labeling and imaging of synaptically connected neuronal 
            networks in vivo using double-deletion-mutant rabies viruses.
  JOURNAL   Nat Neurosci. 2024 Jan 11. doi: 10.1038/s41593-023-01545-8.
  PUBMED    38212587
REFERENCE   2  (bases 1 to 14639)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Neurosci. 2024 Jan 11. doi: 10.1038/s41593-023-01545-8."
COMMENT     Sequence Label: pB-CAG-B19L-IRES-mCherry-WPRE-BGHpA
FEATURES             Location/Qualifiers
     source          1..14639
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(1..105)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(131..586)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(268..289)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     480..499
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     713..735
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     727..744
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     728..744
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     repeat_region   874..936
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     enhancer        1419..1722
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1725..2000
                     /label=chicken beta-actin promoter
     intron          2001..3009
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     2933..2955
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     3017..3036
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             4717..4728
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     misc_feature    9467..10018
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(9611..9628)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     9838..9857
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             10030..10740
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(10175..10193)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     10443..10462
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    10746..11334
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(10799..10819)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(11217..11228)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    11338..11562
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     repeat_region   12093..12127
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(12618..12634)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(12618..12634)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(12631..12653)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    12642..12658
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(12666..12672,12673..12690,12691..12696))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12711..12732
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(12849..12866)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(13020..13608)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(13100..13119)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(13779..14570,14571..14639))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     14402..14421
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
       61 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
      121 agtgccacct aaattgtaag cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa
      181 atcagctcat tttttaacca ataggccgaa atcggcaaaa tcccttataa atcaaaagaa
      241 tagaccgaga tagggttgag tgttgttcca gtttggaaca agagtccact attaaagaac
      301 gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa
      361 ccatcaccct aatcaagttt tttggggtcg aggtgccgta aagcactaaa tcggaaccct
      421 aaagggagcc cccgatttag agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa
      481 gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc
      541 gtaaccacca cacccgccgc gcttaatgcg ccgctacagg gcgcgtccca ttcgccattc
      601 aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg
      661 gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca
      721 cgacgttgta aaacgacggc cagtgagcgc gcctcgttca ttcacgtttt tgaacccgtg
      781 gaggacgggc agactcgcgg tgcaaatgtg ttttacagcg tgatggagca gatgaagatg
      841 ctcgacacgc tgcagaacac gcagctagat taaccctaga aagataatca tattgtgacg
      901 tacgttaaag ataatcatgt gtaaaattga cgcatgtgtt ttatcggtct gtatatcgag
      961 gtttatttat taatttgaat agatattaag ttttattata tttacactta catactaata
     1021 ataaattcaa caaacaattt atttatgttt atttatttat taaaaaaaac aaaaactcaa
     1081 aatttcttct ataaagtaac aaaactttta tgagggacag ccccccccca aagcccccag
     1141 ggatgtaatt acgtccctcc cccgctaggg ggcagcagcg agccgcccgg ggctccgctc
     1201 cggtccggcg ctccccccgc atccccgagc cggcagcgtg cggggacagc ccgggcacgg
     1261 ggaaggtggc acgggatcgc tttcctctga acgcttctcg ctgctctttg agcctgcaga
     1321 cacctggggg gatacgggga aaaggcctcc acggccacta gttattaata gtaatcaatt
     1381 acggggtcat tagttcatag cccatatatg gagttccgcg ttacataact tacggtaaat
     1441 ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt
     1501 cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggacta tttacggtaa
     1561 actgcccact tggcagtaca tcaagtgtat catatgccaa gtacgccccc tattgacgtc
     1621 aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttatg ggactttcct
     1681 acttggcagt acatctacgt attagtcatc gctattacca tgggtcgagg tgagccccac
     1741 gttctgcttc actctcccca tctccccccc ctccccaccc ccaattttgt atttatttat
     1801 tttttaatta ttttgtgcag cgatgggggc gggggggggg ggggcgcgcg ccaggcgggg
     1861 cggggcgggg cgaggggcgg ggcggggcga ggcggagagg tgcggcggca gccaatcaga
     1921 gcggcgcgct ccgaaagttt ccttttatgg cgaggcggcg gcggcggcgg ccctataaaa
     1981 agcgaagcgc gcggcgggcg ggagtcgctg cgttgccttc gccccgtgcc ccgctccgcg
     2041 ccgcctcgcg ccgcccgccc cggctctgac tgaccgcgtt actcccacag gtgagcgggc
     2101 gggacggccc ttctcctccg ggctgtaatt agcgcttggt ttaatgacgg ctcgtttctt
     2161 ttctgtggct gcgtgaaagc cttaaagggc tccgggaggg ccctttgtgc gggggggagc
     2221 ggctcggggg gtgcgtgcgt gtgtgtgtgc gtggggagcg ccgcgtgcgg cccgcgctgc
     2281 ccggcggctg tgagcgctgc gggcgcggcg cggggctttg tgcgctccgc gtgtgcgcga
     2341 ggggagcgcg gccgggggcg gtgccccgcg gtgcgggggg gctgcgaggg gaacaaaggc
     2401 tgcgtgcggg gtgtgtgcgt gggggggtga gcagggggtg tgggcgcggc ggtcgggctg
     2461 taaccccccc ctgcaccccc ctccccgagt tgctgagcac ggcccggctt cgggtgcggg
     2521 gctccgtgcg gggcgtggcg cggggctcgc cgtgccgggc ggggggtggc ggcaggtggg
     2581 ggtgccgggc ggggcggggc cgcctcgggc cggggagggc tcgggggagg ggcgcggcgg
     2641 ccccggagcg ccggcggctg tcgaggcgcg gcgagccgca gccattgcct tttatggtaa
     2701 tcgtgcgaga gggcgcaggg acttcctttg tcccaaatct ggcggagccg aaatctggga
     2761 ggcgccgccg caccccctct agcgggcgcg ggcgaagcgg tgcggcgccg gcaggaagga
     2821 aatgggcggg gagggccttc gtgcgtcgcc gcgccgccgt ccccttctcc atctccagcc
     2881 tcggggctgc cgcaggggga cggctgcctt cgggggggac ggggcagggc ggggttcggc
     2941 ttctggcgtg tgaccggcgg ctctagagcc tctgctaacc atgttcatgc cttcttcttt
     3001 ttcctacagc tcctgggcaa cgtgctggtt attgtgctgt ctcatcattt tggcaaagaa
     3061 ttcgccgcca ccatgctcga tcctggagag gtctatgatg accctattga cccaatcgag
     3121 ttagaggctg aacccagagg aacccccatt gtccccaaca tcttgaggaa ctctgactac
     3181 aatctcaact ctcctttgat agaagatcct gctagactaa tgttagaatg gttaaaaaca
     3241 gggaatagac cttatcggat gactctaaca gacaattgct ccaggtcttt cagagttttg
     3301 aaagattatt tcaagaaggt agatttgggt tctctcaagg tgggcggaat ggctgcacag
     3361 tcaatgattt ctctctggtt atatggtgcc cactctgaat ccaacaggag ccggagatgt
     3421 ataacagact tggcccattt ctattccaag tcgtccccca tagagaagct gttgaatctc
     3481 acgctaggaa atagagggct gagaatcccc ccagagggag tgttaagttg ccttgagagg
     3541 gttgattatg ataatgcatt tggaaggtat cttgccaaca cgtattcctc ttacttgttc
     3601 ttccatgtaa tcaccttata catgaacgcc ctagactggg atgaagaaaa gaccatccta
     3661 gcattatgga aagatttaac ctcagtggac atcgggaagg acttggtaaa gttcaaagac
     3721 caaatatggg gactgctgat cgtgacaaag gactttgttt actcccaaag ttccaattgt
     3781 ctttttgaca gaaactacac acttatgcta aaagatcttt tcttgtctcg cttcaactcc
     3841 ttaatggtct tgctctctcc cccagagccc cgatactcag atgacttgat atctcaacta
     3901 tgccagctgt acattgctgg ggatcaagtc ttgtctatgt gtggaaactc cggctatgaa
     3961 gtcatcaaaa tattggagcc atatgtcgtg aatagtttag tccagagagc agaaaagttt
     4021 aggcctctca ttcattcctt gggagacttt cctgtattta taaaagacaa ggtaagtcaa
     4081 cttgaagaga cgttcggtcc ctgtgcaaga aggttcttta gggctctgga tcaattcgac
     4141 aacatacatg acttggtttt tgtgtttggc tgttacaggc attgggggca cccatatata
     4201 gattatcgaa agggtctgtc aaaactatat gatcaggttc accttaaaaa aatgatagat
     4261 aagtcctacc aggagtgctt agcaagcgac ctagccagga ggatccttag atggggtttt
     4321 gataagtact ccaagtggta tctggattca agattcctag cccgagacca ccccttgact
     4381 ccttatatca aaacccaaac atggccaccc aaacatattg tagacttggt gggggataca
     4441 tggcacaagc tcccgatcac gcagatcttt gagattcctg aatcaatgga tccgtcagaa
     4501 atattggatg acaaatcaca ttctttcacc agaacgagac tagcttcttg gctgtcagaa
     4561 aaccgagggg ggcctgttcc tagcgaaaaa gttattatca cggccctgtc taagccgcct
     4621 gtcaatcccc gagagtttct gaggtctata gacctcggag gattgccaga tgaagacttg
     4681 ataattggcc tcaagccaaa ggaacgggaa ttgaagattg aaggtcgatt ctttgctcta
     4741 atgtcatgga atctaagatt gtattttgtc atcactgaaa aactcttggc caactacatc
     4801 ttgccacttt ttgacgcgct gactatgaca gacaacctga acaaggtgtt taaaaagctg
     4861 atcgacaggg tcaccgggca agggcttttg gactattcaa gggtcacata tgcatttcac
     4921 ctggactatg aaaagtggaa caaccatcaa agattagagt caacagagga tgtattttct
     4981 gtcctagatc aagtgtttgg attgaagaga gtgttttcta gaacacacga gttttttcaa
     5041 aaggcctgga tctattattc agacagatca gacctcatcg ggttacggga ggatcaaata
     5101 tactgcttag atgcgtccaa cggcccaacc tgttggaatg gccaggatgg cgggctagaa
     5161 ggcttacggc agaagggctg gagtctagtc agcttattga tgatagatag agaatctcaa
     5221 atcaggaaca caagaaccaa aatactagct caaggagaca accaggtttt atgtccgaca
     5281 tacatgttgt cgccagggct atctcaagag gggctcctct atgaattgga gagaatatca
     5341 aggaatgcac tttcgatata cagagccgtc gaggaagggg catctaagct agggctgatc
     5401 atcaagaaag aagagaccat gtgtagttat gacttcctca tctatggaaa aacccctttg
     5461 tttagaggta acatattggt gcctgagtcc aaaagatggg ccagagtctc ttgcgtctct
     5521 aatgaccaaa tagtcaacct cgccaatata atgtcgacag tgtccaccaa tgcgctaaca
     5581 gtggcacaac actctcaatc tttgatcaaa ccgatgaggg attttctgct catgtcagta
     5641 caggcagtct ttcactacct gctatttagc ccaatcttaa agggaagagt ttacaagatt
     5701 ctgagcgctg aaggggagag ctttctccta gccatgtcaa ggataatcta tctagatcct
     5761 tctttgggag ggatatctgg aatgtccctc ggaagattcc atatacgaca gttctcagac
     5821 cctgtctctg aagggttatc cttctggaga gagatctggt taagctccca agagtcctgg
     5881 attcacgcgt tgtgtcaaga ggctggaaac ccagatcttg gagagagaac actcgagagc
     5941 ttcactcgcc ttctagaaga tccgaccacc ttaaatatca gaggaggggc cagtcctacc
     6001 attctactca aggatgcaat cagaaaggct ttatatgacg aggtggacaa ggtggaaaat
     6061 tcagagtttc gagaggcaat cctgttgtcc aagacccata gagataattt tatactcttc
     6121 ttaatatctg ttgagcctct gtttcctcga tttctcagtg agctattcag ttcgtctttt
     6181 ttgggaatcc ccgagtcaat cattggattg atacaaaact cccgaacgat aagaaggcag
     6241 tttagaaaga gtctctcaaa aactttagaa gaatccttct acaactcaga gatccacggg
     6301 attagtcgga tgacccagac acctcagagg gttggggggg tgtggccttg ctcttcagag
     6361 agggcagatc tacttaggga gatctcttgg ggaagaaaag tggtaggcac gacagttcct
     6421 cacccttctg agatgttggg attacttccc aagtcctcta tttcttgcac ttgtggagca
     6481 acaggaggag gcaatcctag agtttctgta tcagtactcc cgtcctttga tcagtcattt
     6541 ttttcacgag gccccctaaa gggatacttg ggctcgtcca cctctatgtc gacccagcta
     6601 ttccatgcat gggaaaaagt cactaatgtt catgtggtga agagagctct atcgttaaaa
     6661 gaatctataa actggttcat tactagagat tccaacttgg ctcaagctct aattaggaac
     6721 attatgtctc tgacaggccc tgatttccct ctagaggagg cccctgtctt caaaaggacg
     6781 gggtcagcct tgcataggtt caagtctgcc agatacagcg aaggagggta ttcttctgtc
     6841 tgcccgaacc tcctctctca tatttctgtt agtacagaca ccatgtctga tttgacccaa
     6901 gacgggaaga actacgattt catgttccag ccattgatgc tttatgcaca gacatggaca
     6961 tcagagctgg tacagagaga cacaaggcta agagactcta cgtttcattg gcacctccga
     7021 tgcaacaggt gtgtgagacc cattgacgac gtgaccctgg agacctctca gatcttcgag
     7081 tttccggatg tgtcgaaaag aatatccaga atggtttctg gggctgtgcc tcacttccag
     7141 aggcttcccg atatccgtct gagaccagga gattttgaat ctctaagcgg tagagaaaag
     7201 tctcaccata tcggatcagc tcaggggctc ttatactcaa tcttagtggc aattcacgac
     7261 tcaggataca atgatggaac catcttccct gtcaacatat acggcaaggt ttcccctaga
     7321 gactatttga gagggctcgc aaggggagta ttgataggat cctcgatttg cttcttgaca
     7381 agaatgacaa atatcaatat taatagacct cttgaattgg tctcaggggt aatctcatat
     7441 attctcctga ggctagataa ccatccctcc ttgtacataa tgctcagaga accgtctctt
     7501 agaggagaga tattttctat ccctcagaaa atccccgccg cttatccaac cactatgaaa
     7561 gaaggcaaca gatcaatctt gtgttatctc caacatgtgc tacgctatga gcgagagata
     7621 atcacggcgt ctccagagaa tgactggcta tggatctttt cagactttag aagtgccaaa
     7681 atgacgtacc tatccctcat tacttaccag tctcatcttc tactccagag ggttgagaga
     7741 aacctatcta agagtatgag agataacctg cgacaattga gttctttgat gaggcaggtg
     7801 ctgggcgggc acggagaaga taccttagag tcagacgaca acattcaacg actgctaaaa
     7861 gactctttac gaaggacaag atgggtggat caagaggtgc gccatgcagc tagaaccatg
     7921 actggagatt acagccccaa caagaaggtg tcccgtaagg taggatgttc agaatgggtc
     7981 tgctctgctc aacaggttgc agtctctacc tcagcaaacc cggcccctgt ctcggagctt
     8041 gacataaggg ccctctctaa gaggttccag aaccctttga tctcgggctt gagagtggtt
     8101 cagtgggcaa ccggtgctca ttataagctt aagcctattc tagatgatct caatgttttc
     8161 ccatctctct gccttgtagt tggggacggg tcagggggga tatcaagggc agtcctcaac
     8221 atgtttccag atgccaagct tgtgttcaac agtcttttag aggtgaatga cctgatggct
     8281 tccggaacac atccactgcc tccttcagca atcatgaggg gaggaaatga tatcgtctcc
     8341 agagtgatag atcttgactc aatctgggaa aaaccgtccg acttgagaaa cttggcaacc
     8401 tggaaatact tccagtcagt ccaaaagcag gtcaacatgt cctatgacct cattatttgc
     8461 gatgcagaag ttactgacat tgcatctatc aaccggatca ccctgttaat gtccgatttt
     8521 gcattgtcta tagatggacc actctatttg gtcttcaaaa cttatgggac tatgctagta
     8581 aatccaaact acaaggctat tcaacacctg tcaagagcgt tcccctcggt cacagggttt
     8641 atcacccaag taacttcgtc tttttcatct gagctctacc tccgattctc caaacgaggg
     8701 aagtttttca gagatgctga gtacttgacc tcttccaccc ttcgagaaat gagccttgtg
     8761 ttattcaatt gtagcagccc caagagtgag atgcagagag ctcgttcctt gaactatcag
     8821 gatcttgtga gaggatttcc tgaagaaatc atatcaaatc cttacaatga gatgatcata
     8881 actctgattg acagtgatgt agaatctttt ctagtccaca agatggttga tgatcttgag
     8941 ttacagaggg gaactctgtc taaagtggct atcattatag ccatcatgat agttttctcc
     9001 aacagagtct tcaacgtttc caaaccccta actgacccct cgttctatcc accgtctgat
     9061 cccaaaatcc tgaggcactt caacatatgt tgcagtacta tgatgtatct atctactgct
     9121 ttaggtgacg tccctagctt cgcaagactt cacgacctgt ataacagacc tataacttat
     9181 tacttcagaa agcaagtcat tcgagggaac gtttatctat cttggagttg gtccaacgac
     9241 acctcagtgt tcaaaagggt agcctgtaat tctagcctga gtctgtcatc tcactggatc
     9301 aggttgattt acaagatagt gaagactacc agactcgttg gcagcatcaa ggatctatcc
     9361 agagaagtgg aaagacacct tcataggtac aacaggtgga tcaccctaga ggatatcaga
     9421 tctagatcat ccctactaga ctacagttgc ctgtgagcgg ccgctaacgt tactggccga
     9481 agccgcttgg aataaggccg gtgtgcgttt gtctatatgt tattttccac catattgccg
     9541 tcttttggca atgtgagggc ccggaaacct ggccctgtct tcttgacgag cattcctagg
     9601 ggtctttccc ctctcgccaa aggaatgcaa ggtctgttga atgtcgtgaa ggaagcagtt
     9661 cctctggaag cttcttgaag acaaacaacg tctgtagcga ccctttgcag gcagcggaac
     9721 cccccacctg gcgacaggtg cctctgcggc caaaagccac gtgtataaga tacacctgca
     9781 aaggcggcac aaccccagtg ccacgttgtg agttggatag ttgtggaaag agtcaaatgg
     9841 ctctcctcaa gcgtattcaa caaggggctg aaggatgccc agaaggtacc ccattgtatg
     9901 ggatctgatc tggggcctcg gtgcacatgc tttacatgtg tttagtcgag gttaaaaaac
     9961 gtctaggccc cccgaaccac ggggacgtgg ttttcctttg aaaaacacga tgataatatg
    10021 gccacaacca tggtgagcaa gggcgaggag gataacatgg ccatcatcaa ggagttcatg
    10081 cgcttcaagg tgcacatgga gggctccgtg aacggccacg agttcgagat cgagggcgag
    10141 ggcgagggcc gcccctacga gggcacccag accgccaagc tgaaggtgac caagggtggc
    10201 cccctgccct tcgcctggga catcctgtcc cctcagttca tgtacggctc caaggcctac
    10261 gtgaagcacc ccgccgacat ccccgactac ttgaagctgt ccttccccga gggcttcaag
    10321 tgggagcgcg tgatgaactt cgaggacggc ggcgtggtga ccgtgaccca ggactcctcc
    10381 ctgcaggacg gcgagttcat ctacaaggtg aagctgcgcg gcaccaactt cccctccgac
    10441 ggccccgtaa tgcagaagaa gaccatgggc tgggaggcct cctccgagcg gatgtacccc
    10501 gaggacggcg ccctgaaggg cgagatcaag cagaggctga agctgaagga cggcggccac
    10561 tacgacgctg aggtcaagac cacctacaag gccaagaagc ccgtgcagct gcccggcgcc
    10621 tacaacgtca acatcaagtt ggacatcacc tcccacaacg aggactacac catcgtggaa
    10681 cagtacgaac gcgccgaggg ccgccactct accggcggca tggacgagct gtacaagtaa
    10741 gatccaatca acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg
    10801 ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt
    10861 cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct ctttatgagg
    10921 agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc
    10981 ccactggttg gggcattgcc accacctgtc agctcctttc cgggactttc gctttccccc
    11041 tccctattgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg acaggggctc
    11101 ggctgttggg cactgacaat tccgtggtgt tgtcggggaa gctgacgtcc tttccatggc
    11161 tgctcgcctg tgttgccacc tggattctgc gcgggacgtc cttctgctac gtcccttcgg
    11221 ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc
    11281 gtcttcgcct tcgccctcag acgagtcgga tctccctttg ggccgcctcc ccgcctgctg
    11341 tgccttctag ttgccagcca tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg
    11401 aaggtgccac tcccactgtc ctttcctaat aaaatgagga aattgcatcg cattgtctga
    11461 gtaggtgtca ttctattctg gggggtgggg tggggcagga cagcaagggg gaggattggg
    11521 aagacaatag caggcatgct ggggatgcgg tgggctctat ggagatccgc ggcccattgg
    11581 tatggctttt tccccgtatc cccccaggtg tctgcaggct caaagagcag cgagaagcgt
    11641 tcagaggaaa gcgatcccgt gccaccttcc ccgtgcccgg gctgtccccg cacgctgccg
    11701 gctcggggat gcggggggag cgccggaccg gagcggagcc ccgggcggct cgctgctgcc
    11761 ccctagcggg ggagggacgt aattacatcc ctgggggctt tggggggggg ctgtccctga
    11821 tatctataac aagaaaatat atatataata agttatcacg taagtagaac atgaaataac
    11881 aatataatta tcgtatgagt taaatcttaa aagtcacgta aaagataatc atgcgtcatt
    11941 ttgactcacg cggtcgttat agttcaaaat cagtgacact taccgcattg acaagcacgc
    12001 ctcacgggag ctccaagcgg cgactgagat gtcctaaatg cacagcgacg gattcgcgct
    12061 atttagaaag agagagcaat atttcaagaa tgcatgcgtc aattttacgc agactatctt
    12121 tctagggtta atctagctgc atcaggatca tatcgtcggg tcttttttcc ggctcagtca
    12181 tcgcccaagc tggcgctatc tgggcatcgg ggaggaagaa gcccgtgcct tttcccgcga
    12241 ggttgaagcg gcatggaaag agtttgccga ggatgactgc tgctgcattg acgttgagcg
    12301 aaaacgcacg tttaccatga tgattcggga aggtgtggcc atgcacgcct ttaacggtga
    12361 actgttcgtt caggccacct gggataccag ttcgtcgcgg cttttccgga cacagttccg
    12421 gatggtcagc ccgaagcgca tcagcaaccc gaacaatacc ggcgacagcc ggaactgccg
    12481 tgccggtgtg cagattaatg acagcggtgc ggcgctggga tattacgtca gcgaggacgg
    12541 gtatcctggc tggatgccgc agaaatggac atggataccc cgtgagttac ccggcgggcg
    12601 cgcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt
    12661 ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc
    12721 taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc
    12781 cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct
    12841 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca
    12901 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac
    12961 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
    13021 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
    13081 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
    13141 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
    13201 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
    13261 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
    13321 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
    13381 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
    13441 aactacggct acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc
    13501 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
    13561 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg
    13621 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc
    13681 atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa
    13741 tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag
    13801 gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg
    13861 tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga
    13921 gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag
    13981 cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa
    14041 gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc
    14101 atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca
    14161 aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg
    14221 atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat
    14281 aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc
    14341 aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg
    14401 gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg
    14461 gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt
    14521 gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca
    14581 ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcat
//
