LOCUS       sequence-346685-        6800 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses Myc Mutant (delta Myc BoxIIb) tagged with His and V5 in 
            Mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6800)
  AUTHORS   Tu WB, Shiah YJ, Lourenco C, Mullen PJ, Dingar D, Redel C, Tamachi 
            A, Ba-Alawi W, Aman A, Al-Awar R, Cescon DW, Haibe-Kains B, 
            Arrowsmith CH, Raught B, Boutros PC, Penn LZ
  TITLE     MYC Interacts with the G9a Histone Methyltransferase to Drive 
            Transcriptional Repression and Tumorigenesis.
  JOURNAL   Cancer Cell. 2018 Oct 8;34(4):579-595.e8. doi: 
            10.1016/j.ccell.2018.09.001.
  PUBMED    30300580
REFERENCE   2  (bases 1 to 6800)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ccell.2018.09"; journalName: "Cancer Cell"; date: 
            "2018-10-8- 8"; volume: "34"; issue: "4"; pages: "579-595.e8"
COMMENT     Sequence Label: pcDNA3.1 His-V5 Myc dMBIIb
FEATURES             Location/Qualifiers
     source          1..6800
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(join(524..1315,1316..1384))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1147..1166
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1385..1489)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(1564..1583)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        1755..2134
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2135..2338
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2289..2309
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2383..2402
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2383..2401
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             3357..3383
                     /codon_start=1
                     /product="nuclear localization signal of human c-Myc 
                     proto-oncogene (Dang and Lee, 1988)"
                     /label=c-myc NLS
                     /translation="PAAKRVKLD"
     CDS             3627..3656
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             3741..3782
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             3792..3809
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     promoter        complement(3873..3891)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(3874..3891)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(3911..3928)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3917..4141
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4187..4615
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4274..4293)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4484..4505
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4624..4644)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4629..4958
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4809..4944
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     4871..4890
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             5025..5819
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5079..5098)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5689..5708
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    5993..6114
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6030..6049)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6084..6103
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6163..6179)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6163..6179)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6176..6198)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6187..6203
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6211..6217,6218..6235,6236..6241))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6256..6277
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6394..6411)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(join(6565..6800,1..353))
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6645..6664)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg
       61 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca
      121 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt
      181 ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag
      241 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg
      301 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc
      361 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt
      421 tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt
      481 ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca
      541 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg
      601 tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac
      661 cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg
      721 ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc
      781 gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta
      841 caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac
      901 gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc
      961 ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac
     1021 tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact
     1081 caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa
     1141 tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt
     1201 cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca
     1261 ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa
     1321 aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac
     1381 tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg
     1441 gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc
     1501 gaaaagtgcc acctgacgtc gacggatcgg gagatctccc gatcccctat ggtgcactct
     1561 cagtacaatc tgctctgatg ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt
     1621 ggaggtcgct gagtagtgcg cgagcaaaat ttaagctaca acaaggcaag gcttgaccga
     1681 caattgcatg aagaatctgc ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc
     1741 cagatatacg cgttgacatt gattattgac tagttattaa tagtaatcaa ttacggggtc
     1801 attagttcat agcccatata tggagttccg cgttacataa cttacggtaa atggcccgcc
     1861 tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt
     1921 aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca
     1981 cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg tcaatgacgg
     2041 taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc ctacttggca
     2101 gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc agtacatcaa
     2161 tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa
     2221 tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta acaactccgc
     2281 cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa gcagagctct
     2341 ctggctaact agagaaccca ctgcttactg gcttatcgaa attaatacga ctcactatag
     2401 ggagacccaa gcttggtacc gagctcggat ccaccatgcc cctcaacgtt agcttcacca
     2461 acaggaacta tgacctcgac tacgactcgg tgcagccgta tttctactgc gacgaggagg
     2521 agaacttcta ccagcagcag cagcagagcg agctgcagcc cccggcgccc agcgaggata
     2581 tctggaagaa attcgagctg ctgcccaccc cgcccctgtc ccctagccgc cgctccgggc
     2641 tctgctcgcc ctcctacgtt gcggtcacac ccttctccct tcggggagac aacgacggcg
     2701 gtggcgggag cttctccacg gccgaccagc tggagatggt gaccgagctg ctgggaggag
     2761 acatggtgaa ccagagtttc atctgcgacc cggacgacga gaccttcatc aaaaacatca
     2821 tcatccagga ctgtatgtgg agcggcttct cggccgccgc caagctcgtc tcagagaagc
     2881 tggcctccta ccaggctgcg cgcaaagaca gcggcagccc gaaccccgcc cgcggccaca
     2941 gcgtctgctc cacctccagc ttgtacctgc aggatctgag cgccgccgcc tcagagtgca
     3001 tcgacccctc ggtggtcttc ccctaccctc tcaacgacag cagctcgccc aagtcctgcg
     3061 cctcgcaaga ctccagcgcc ttctctccgt cctcggattc tctgctctcc tcgacggagt
     3121 cctccccgca gggcagcccc gagcccctgg tgctccatga ggagacaccg cccaccacca
     3181 gcagcgactc tgaggaggaa caagaagatc aggctcctgg caaaaggtca gagtctggat
     3241 caccttctgc tggaggccac agcaaacctc ctcacagccc actggtcctc aagaggtgcc
     3301 acgtctccac acatcagcac aactacgcag cgcctccctc cactcggaag gactatcctg
     3361 ctgccaagag ggtcaagttg gacagtgtca gagtcctgag acagatcagc aacaaccgaa
     3421 aatgcaccag ccccaggtcc tcggacaccg aggagaatgt caagaggcga acacacaacg
     3481 tcttggagcg ccagaggagg aacgagctaa aacggagctt ttttgccctg cgtgaccaga
     3541 tcccggagtt ggaaaacaat gaaaaggccc ccaaggtagt tatccttaaa aaagccacag
     3601 catacatcct gtccgtccaa gcagaggagc aaaagctcat ttctgaagag gacttgttgc
     3661 ggaaacgacg agaacagttg aaacacaaac ttgaacagct acggaactct tgtgcgggtc
     3721 tagagggccc gcggttcgaa ggtaagccta tccctaaccc tctcctcggt ctcgattcta
     3781 cgcgtaccgg tcatcatcac catcaccatt gagaattctg cagatatcca tcacactggc
     3841 ggccgctcga gcatgcatct agagggccct attctatagt gtcacctaaa tgctagagct
     3901 cgctgatcag cctcgactgt gccttctagt tgccagccat ctgttgtttg cccctccccc
     3961 gtgccttcct tgaccctgga aggtgccact cccactgtcc tttcctaata aaatgaggaa
     4021 attgcatcgc attgtctgag taggtgtcat tctattctgg ggggtggggt ggggcaggac
     4081 agcaaggggg aggattggga agacaatagc aggcatgctg gggatgcggt gggctctatg
     4141 gcttctgagg cggaaagaac cagctggggc tctagggggt atccccacgc gccctgtagc
     4201 ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc
     4261 gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt
     4321 ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac
     4381 ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag
     4441 acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa
     4501 actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg
     4561 atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattaattc
     4621 tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg ctccccagca ggcagaagta
     4681 tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag
     4741 caggcagaag tatgcaaagc atgcatctca attagtcagc aaccatagtc ccgcccctaa
     4801 ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc catggctgac
     4861 taattttttt tatttatgca gaggccgagg ccgcctctgc ctctgagcta ttccagaagt
     4921 agtgaggagg cttttttgga ggcctaggct tttgcaaaaa gctcccggga gcttgtatat
     4981 ccattttcgg atctgatcaa gagacaggat gaggatcgtt tcgcatgatt gaacaagatg
     5041 gattgcacgc aggttctccg gccgcttggg tggagaggct attcggctat gactgggcac
     5101 aacagacaat cggctgctct gatgccgccg tgttccggct gtcagcgcag gggcgcccgg
     5161 ttctttttgt caagaccgac ctgtccggtg ccctgaatga actgcaggac gaggcagcgc
     5221 ggctatcgtg gctggccacg acgggcgttc cttgcgcagc tgtgctcgac gttgtcactg
     5281 aagcgggaag ggactggctg ctattgggcg aagtgccggg gcaggatctc ctgtcatctc
     5341 accttgctcc tgccgagaaa gtatccatca tggctgatgc aatgcggcgg ctgcatacgc
     5401 ttgatccggc tacctgccca ttcgaccacc aagcgaaaca tcgcatcgag cgagcacgta
     5461 ctcggatgga agccggtctt gtcgatcagg atgatctgga cgaagagcat caggggctcg
     5521 cgccagccga actgttcgcc aggctcaagg cgcgcatgcc cgacggcgag gatctcgtcg
     5581 tgacccatgg cgatgcctgc ttgccgaata tcatggtgga aaatggccgc ttttctggat
     5641 tcatcgactg tggccggctg ggtgtggcgg accgctatca ggacatagcg ttggctaccc
     5701 gtgatattgc tgaagagctt ggcggcgaat gggctgaccg cttcctcgtg ctttacggta
     5761 tcgccgctcc cgattcgcag cgcatcgcct tctatcgcct tcttgacgag ttcttctgag
     5821 cgggactctg gggttcgaaa tgaccgacca agcgacgccc aacctgccat cacgagattt
     5881 cgattccacc gccgccttct atgaaaggtt gggcttcgga atcgttttcc gggacgccgg
     5941 ctggatgatc ctccagcgcg gggatctcat gctggagttc ttcgcccacc ccaacttgtt
     6001 tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc
     6061 atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt
     6121 ctgtataccg tcgacctcta gctagagctt ggcgtaatca tggtcatagc tgtttcctgt
     6181 gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa
     6241 agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc
     6301 tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag
     6361 aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt
     6421 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga
     6481 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg
     6541 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa
     6601 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt
     6661 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct
     6721 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct
     6781 cagttcggtg taggtcgttc
//
