LOCUS       sequence-346717-        9423 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Constitutive expression of a gRNA-tRNA array for macsGESTALT 
            editing.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9423)
  AUTHORS   Simeonov KP, Byrns CN, Clark ML, Norgard RJ, Martin B, Stanger BZ, 
            Shendure J, McKenna A, Lengner CJ
  TITLE     Single-cell lineage tracing of metastatic cancer reveals selection 
            of hybrid EMT states.
  JOURNAL   Cancer Cell. 2021 Aug 9;39(8):1150-1162.e9. doi: 
            10.1016/j.ccell.2021.05.005. Epub 2021 Jun 10.
  PUBMED    34115987
REFERENCE   2  (bases 1 to 9423)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ccell.2021.05.005"; journalName: "Cancer Cell"; date: 
            "2021-08-9- 9"; volume: "39"; issue: "8"; pages: "1150-1162.e9"
COMMENT     Sequence Label: pUltra-U6-gRNAs1-5
FEATURES             Location/Qualifiers
     source          1..9423
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             236..413
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    457..582
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1075..1308
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1493..1537
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1835..1952
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2008..2256
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     2008..2028
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     2179..2198
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        2278..2353
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     misc_RNA        2452..2527
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     misc_RNA        2626..2701
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     misc_RNA        2800..2875
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     misc_RNA        2974..3049
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        3113..4324
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     4161..4180
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     regulatory      4357..4366
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(4363..4365,4366..4368,4369..5079)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(4363..4365,4366..4368,4369..5079)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4408..4429)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4669..4688)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5016..5037
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             5089..5145
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             5167..5220
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     misc_feature    5264..5852
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5317..5337)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5735..5746)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5943..6176
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6248..6369
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6285..6304)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6339..6358
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6409..6544
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6564..6583)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6565..6583)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6593..6610)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6593..6609)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6602..6624)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6754..7209
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6841..6860)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7051..7072
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7238..7342
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7343..7411,7412..8203)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7561..7580)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8374..8962
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8863..8882
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9116..9133
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9250..9271
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9286..9291,9292..9309,9310..9316)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9324..9340
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9329..9351
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9348..9364
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9348..9364
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     9383..9403
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        9385..9403
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 agcttaatgt agtcttatgc aatactcttg tagtcttgca acatggtaac gatgagttag
       61 caacatgcct tacaaggaga gaaaaagcac cgtgcatgcc gattggtgga agtaaggtgg
      121 tacgatcgtg ccttattagg aaggcaacag acgggtctga catggattgg acgaaccact
      181 gaattgccgc attgcagaga tattgtattt aagtgcctag ctcgatacaa taaacgggtc
      241 tctctggtta gaccagatct gagcctggct ctctggctaa ctagggaacc cactgcttaa
      301 gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc
      361 tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc
      421 ccgaacaggg acctgaaagc gaaagggaaa ccagagctct ctcgacgcag gactcggctt
      481 gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg
      541 actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga
      601 attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta
      661 aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta
      721 gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga
      781 tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg
      841 atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt
      901 aagaccaccg cacagcaagc ggccgctgat cttcagacct ggaggaggag atatgaggga
      961 caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc
     1021 acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc
     1081 tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct
     1141 gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag
     1201 ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca
     1261 ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg
     1321 ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa
     1381 atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatcggcac tgcgtgcgcc
     1801 aattctgcag acaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg
     1861 ggtacagtgc aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat
     1921 tacaaaaaca aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagatc
     1981 cagtttggtt aatggcgcgc cggtaccgag ggcctatttc ccatgattcc ttcatatttg
     2041 catatacgat acaaggctgt tagagagata attggaatta atttgactgt aaacacaaag
     2101 atattagtac aaaatacgtg acgtagaaag taataatttc ttgggtagtt tgcagtttta
     2161 aaattatgtt ttaaaatgga ctatcatatg cttaccgtaa cttgaaagta tttcgatttc
     2221 ttggctttat atatcttgtg gaaaggacga aacaccgtta gagcctggct ggtctccgtt
     2281 ttagagctag aaatagcaag ttaaaataag gctagtccgt tatcaacttg aaaaagtggc
     2341 accgagtcgg tgctaacaaa gcaccagtgg tctagtggta gaatagtacc ctgccacggt
     2401 acagacccgg gttcgattcc cggctggtgc acttaatgag caattcctct cgttttagag
     2461 ctagaaatag caagttaaaa taaggctagt ccgttatcaa cttgaaaaag tggcaccgag
     2521 tcggtgctaa caaagcacca gtggtctagt ggtagaatag taccctgcca cggtacagac
     2581 ccgggttcga ttcccggctg gtgcaagtat tgctatctag gataagtttt agagctagaa
     2641 atagcaagtt aaaataaggc tagtccgtta tcaacttgaa aaagtggcac cgagtcggtg
     2701 ctaacaaagc accagtggtc tagtggtaga atagtaccct gccacggtac agacccgggt
     2761 tcgattcccg gctggtgcag gtcctgcaag gattattatg ttttagagct agaaatagca
     2821 agttaaaata aggctagtcc gttatcaact tgaaaaagtg gcaccgagtc ggtgctaaca
     2881 aagcaccagt ggtctagtgg tagaatagta ccctgccacg gtacagaccc gggttcgatt
     2941 cccggctggt gcattataat caactgcctc ttcgttttag agctagaaat agcaagttaa
     3001 aataaggcta gtccgttatc aacttgaaaa agtggcaccg agtcggtgct tttttgccta
     3061 cctggagcct gagagttgtt cgatatcatt aacccgtgtc ggctccagat ctggcctccg
     3121 cgccgggttt tggcgcctcc cgcgggcgcc cccctcctca cggcgagcgc tgccacgtca
     3181 gacgaagggc gcagcgagcg tcctgatcct tccgcccgga cgctcaggac agcggcccgc
     3241 tgctcataag actcggcctt agaaccccag tatcagcaga aggacatttt aggacgggac
     3301 ttgggtgact ctagggcact ggttttcttt ccagagagcg gaacaggcga ggaaaagtag
     3361 tcccttctcg gcgattctgc ggagggatct ccgtggggcg gtgaacgccg atgattatat
     3421 aaggacgcgc cgggtgtggc acagctagtt ccgtcgcagc cgggatttgg gtcgcggttc
     3481 ttgtttgtgg atcgctgtga tcgtcacttg gtgagtagcg ggctgctggg ctggccgggg
     3541 ctttcgtggc cgccgggccg ctcggtggga cggaagcgtg tggagagacc gccaagggct
     3601 gtagtctggg tccgcgagca aggttgccct gaactggggg ttggggggag cgcagcaaaa
     3661 tggcggctgt tcccgagtct tgaatggaag acgcttgtga ggcgggctgt gaggtcgttg
     3721 aaacaaggtg gggggcatgg tgggcggcaa gaacccaagg tcttgaggcc ttcgctaatg
     3781 cgggaaagct cttattcggg tgagatgggc tggggcacca tctggggacc ctgacgtgaa
     3841 gtttgtcact gactggagaa ctcggtttgt cgtctgttgc gggggcggca gttatggcgg
     3901 tgccgttggg cagtgcaccc gtacctttgg gagcgcgcgc cctcgtcgtg tcgtgacgtc
     3961 acccgttctg ttggcttata atgcagggtg gggccacctg ccggtaggtg tgcggtaggc
     4021 ttttctccgt cgcaggacgc agggttcggg cctagggtag gctctcctga atcgacaggc
     4081 gccggacctc tggtgagggg agggataagt gaggcgtcag tttctttggt cggttttatg
     4141 tacctatctt cttaagtagc tgaagctccg gttttgaact atgcgctcgg ggttggcgag
     4201 tgtgttttgt gaagtttttt aggcaccttt tgaaatgtaa tcatttgggt caatatgtaa
     4261 ttttcagtgt tagactagta aattgtccgc taaattctgg ccgtttttgg cttttttgtt
     4321 agacgaagct tgggctgcag gtccgatcca ccggtcgcca ccatggtgag caagggcgag
     4381 gagctgttca ccggggtggt gcccatcctg gtcgagctgg acggcgacgt aaacggccac
     4441 aagttcagcg tgtccggcga gggcgagggc gatgccacct acggcaagct gaccctgaag
     4501 ttcatctgca ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac caccctgacc
     4561 tacggcgtgc agtgcttcag ccgctacccc gaccacatga agcagcacga cttcttcaag
     4621 tccgccatgc ccgaaggcta cgtccaggag cgcaccatct tcttcaagga cgacggcaac
     4681 tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg catcgagctg
     4741 aagggcatcg acttcaagga ggacggcaac atcctggggc acaagctgga gtacaactac
     4801 aacagccaca acgtctatat catggccgac aagcagaaga acggcatcaa ggtgaacttc
     4861 aagatccgcc acaacatcga ggacggcagc gtgcagctcg ccgaccacta ccagcagaac
     4921 acccccatcg gcgacggccc cgtgctgctg cccgacaacc actacctgag cacccagtcc
     4981 gccctgagca aagaccccaa cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc
     5041 gccgccggga tcactctcgg catggacgag ctgtacaagg gatctggagc aacaaacttc
     5101 tcactactca aacaagcagg tgacgtggag gagaatcccg ggccttctag aggatccggc
     5161 tccggcgagg gcaggggaag tctactaaca tgcggggacg tggaggaaaa tcccggccca
     5221 gctagcgtgt gatcagaatt cgttccggag tcgtcgactc gacaatcaac ctctggatta
     5281 caaaatttgt gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg
     5341 atacgctgct ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc
     5401 ctccttgtat aaatcctggt tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca
     5461 acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc actggttggg gcattgccac
     5521 cacctgtcag ctcctttccg ggactttcgc tttccccctc cctattgcca cggcggaact
     5581 catcgccgcc tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc
     5641 cgtggtgttg tcggggaagc tgacgtcctt tccatggctg ctcgcctgtg ttgccacctg
     5701 gattctgcgc gggacgtcct tctgctacgt cccttcggcc ctcaatccag cggaccttcc
     5761 ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcat cttcgccttc gccctcagac
     5821 gagtcggatc tccctttggg ccgcctcccc gcctggaatt aattcgagct cggtaccttt
     5881 aagaccaatg acttacaagg cagctgtaga tcttagccac tttttaaaag aaaagggggg
     5941 actggaaggg ctacgtaact cccaacgaag acaagatctg ctttttgctt gtactgggtc
     6001 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct
     6061 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga
     6121 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtag
     6181 tagttcatgt catcttatta ttcagtattt ataacttgca aagaaatgaa tatcagagag
     6241 tgagaggaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa
     6301 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa
     6361 tgtatcttat catgtctggc tctagctatc ccgcccctaa ctccgcccat cccgccccta
     6421 actccgccca gttccgccca ttctccgccc catggctgac taattttttt tatttatgca
     6481 gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga
     6541 ggcctaggga cgtacccaat tcgccctata gtgagtcgta ttacgcgcgc tcactggccg
     6601 tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat cgccttgcag
     6661 cacatccccc tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc
     6721 aacagttgcg cagcctgaat ggcgaatggc gcgacgcgcc ctgtagcggc gcattaagcg
     6781 cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg
     6841 ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc
     6901 taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa
     6961 aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc
     7021 ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac
     7081 tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt tcggcctatt
     7141 ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa atattaacgt
     7201 ttacaatttc ccaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt
     7261 tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca
     7321 ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt
     7381 ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga
     7441 tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa
     7501 gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct
     7561 gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat
     7621 acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga
     7681 tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc
     7741 caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat
     7801 gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa
     7861 cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac
     7921 tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa
     7981 agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc
     8041 tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc
     8101 ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag
     8161 acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta
     8221 ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa
     8281 gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc
     8341 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat
     8401 ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga
     8461 gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt
     8521 ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata
     8581 cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac
     8641 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg
     8701 ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg
     8761 tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag
     8821 cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct
     8881 ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc
     8941 aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt
     9001 ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg
     9061 tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga
     9121 gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg
     9181 gccgattcat taatgcagct ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg
     9241 caacgcaatt aatgtgagtt agctcactca ttaggcaccc caggctttac actttatgct
     9301 tccggctcgt atgttgtgtg gaattgtgag cggataacaa tttcacacag gaaacagcta
     9361 tgaccatgat tacgccaagc gcgcaattaa ccctcactaa agggaacaaa agctggagct
     9421 gca
//
